Efeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11225 |
Resumo: | Selenium is an element that can modulate the dopaminergic neurotransmission. Studies show that diphenyl diselenide, an organic compound of selenium, has antioxidant activity improves depressive-like behavior and reduce the activity of the enzyme monoamine oxidase (MAO). However, there are few studies concerning about possible alterations of diphenyl diselenide in dopaminergic system. Thus, the purpose of the present study was to evaluate the effects of acute and sub-chronic treatment of diphenyl diselenide on amphetamine-induced behavioral and biochemical alterations in mice. In the acute treatment, the mice were treated with diphenyl diselenide (5 and 10 mg/kg, s.c.) or vehicle (10% Tween 80, s.c.) 30 min before administration of amphetamine (1.25 mg/kg, i.p.). After 25 min, locomotor activity was assessed with an open field and, also, the time of stereotypy and immobility was assessed in a glass cage. Sub-chronic treatment was conducted with seven administrations of diphenyl diselenide (5 and 10 mg/kg, s.c.), or its vehicle being one administration per day. On the eighth day, amphetamine (1.25 mg/kg, i.p.) was administered and the behavioral tests were conducted after 25 min. In both treatments ex vivo tests were performed: isoform activity MAO-A and MAO-B, and measurement of total protein and non-protein thiol levels, oxidation of diclorofluorescein. Amphetamine increased the number of crossing and rearing in the open field test and diphenyl diselenide prevented only the increase in the number of crossings when acutely administered to mice. Furthermore, amphetamine increased the time of immobility and stereotypy in mice. Diphenyl diselenide did not prevent these effects. By contrary, at 10 mg/kg, sub-chronic administration of diphenyl diselenide increased per se the time of immobility and stereotypy. It was also found a positive correlation between immobility and stereotypy in acute and sub-chronic treatment with diphenyl diselenide. It was also detected a decrease in brain MAO-B activity caused by sub-chronic treatment with diphenyl diselenide either alone or in combination of amphetamine. Any change was detected in oxidative stress parameters. In conclusion, sub-chronic administration of diphenyl diselenide can promote a behavioral sensitization that seems to be, at least in part, dependent of MAO-B inhibition. |
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2015-02-262015-02-262012-09-19FIGUEIRA, Fernanda Hernandes. Effect of diphenyl diselenide on behavioural and biochemical alterations induced by amphetamine in mice. 2012. 54 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/11225Selenium is an element that can modulate the dopaminergic neurotransmission. Studies show that diphenyl diselenide, an organic compound of selenium, has antioxidant activity improves depressive-like behavior and reduce the activity of the enzyme monoamine oxidase (MAO). However, there are few studies concerning about possible alterations of diphenyl diselenide in dopaminergic system. Thus, the purpose of the present study was to evaluate the effects of acute and sub-chronic treatment of diphenyl diselenide on amphetamine-induced behavioral and biochemical alterations in mice. In the acute treatment, the mice were treated with diphenyl diselenide (5 and 10 mg/kg, s.c.) or vehicle (10% Tween 80, s.c.) 30 min before administration of amphetamine (1.25 mg/kg, i.p.). After 25 min, locomotor activity was assessed with an open field and, also, the time of stereotypy and immobility was assessed in a glass cage. Sub-chronic treatment was conducted with seven administrations of diphenyl diselenide (5 and 10 mg/kg, s.c.), or its vehicle being one administration per day. On the eighth day, amphetamine (1.25 mg/kg, i.p.) was administered and the behavioral tests were conducted after 25 min. In both treatments ex vivo tests were performed: isoform activity MAO-A and MAO-B, and measurement of total protein and non-protein thiol levels, oxidation of diclorofluorescein. Amphetamine increased the number of crossing and rearing in the open field test and diphenyl diselenide prevented only the increase in the number of crossings when acutely administered to mice. Furthermore, amphetamine increased the time of immobility and stereotypy in mice. Diphenyl diselenide did not prevent these effects. By contrary, at 10 mg/kg, sub-chronic administration of diphenyl diselenide increased per se the time of immobility and stereotypy. It was also found a positive correlation between immobility and stereotypy in acute and sub-chronic treatment with diphenyl diselenide. It was also detected a decrease in brain MAO-B activity caused by sub-chronic treatment with diphenyl diselenide either alone or in combination of amphetamine. Any change was detected in oxidative stress parameters. In conclusion, sub-chronic administration of diphenyl diselenide can promote a behavioral sensitization that seems to be, at least in part, dependent of MAO-B inhibition.O selênio é um elemento químico capaz importante para o funcionamento celular, envolvido também na modulação do sistema dopaminérgico. Estudos mostram que o disseleneto de difenila, um composto orgânico de selênio, possui atividade antioxidante, melhora o comportamento tipo-depressivo relacionado à inibição da atividade da enzima monoamino oxidase (MAO). No entanto, existem poucos estudos acerca dos efeitos do disseleneto de difenila sobre o sistema dopaminérgico. Desta forma, o objetivo do presente estudo foi avaliar os efeitos do tratamento agudo e sub-crônico do disseleneto de difenila sobre as alterações bioquímicas e comportamentais induzidas por anfetamina em camundongos. No tratamento agudo, os camundongos foram tratados com disseleneto de difenila (5 e 10 mg/kg, s.c.) ou veículo (10% de tween 80, s.c.) 30 minutos antes da administração de anfetamina (1,25 mg/kg, i.p.). Após 25 minutos, foram avaliados a atividade locomotora através do teste de campo aberto, além do tempo de estereotipia e imobilidade, avaliado em caixa espelhada. O tratamento subcrônico foi realizado com sete administrações de disseleneto de difenila ou veículo (5 e 10 mg/kg, s.c.) sendo uma administração por dia. No oitavo dia foi administrada a anfetamina (1,25 mg/kg, i.p.) e realizados os testes comportamentais 25 minutos após. Em ambos os tratamentos os testes ex-vivo realizados foram: atividade das isoformas MAO-A e MAO-B, níveis de tióis totais e não-protéico, oxidação da diclorofluoresceína. O tratamento com anfetamina aumentou o número de cruzamentos e de levantadas no teste do campo aberto e o disseleneto de difenila preveniu somente o número de cruzamentos quando administrado agudamente aos camundongos. Além disso, o tratamento com anfetamina aumentou o tempo de imobilidade e estereotipia em camundongos. O disseleneto de difenila não preveniu estes efeitos. Pelo contrário, na dose de 10 mg/kg, a administração subcrônica de disseleneto de difenila aumentou per se o tempo de imobilidade e de estereotipia. Uma correlação positiva entre o tempo de estereotipia e de imobilidade foi também encontrada tanto para o tratamento agudo como subcrônico com disseleneto de difenila. Também foi detectada uma diminuição na atividade cerebral da MAO-B causada pelo tratamento subcrônico com disseleneto de difenila tanto per se quanto em combinação com a anfetamina. Não foram encontradas alterações em parâmetros de estresse oxidativo. Em conclusão, o tratamento subcrônico com disseleneto de difenila pode promover uma sensibilização comportamental que parece ser, pelo menos em parte, dependente da inibição da MAO-B.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaDisseleneto de difenilaMonoamino oxidaseEstereotipiaAnfetaminaDiphenyl diselenideMonoamine oxidaseStereotypyAmphetamineCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongosEffect of diphenyl diselenide on behavioural and biochemical alterations induced by amphetamine in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisFachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306Wagner, Carolinehttp://lattes.cnpq.br/4004565241849091Pereira, Romaiana Picadahttp://lattes.cnpq.br/3277439471060779http://lattes.cnpq.br/7299721428462157Figueira, Fernanda Hernandes200800000002400500300500500e99bf03a-61e7-46c9-97d1-a7255476d47f4fd7db88-3ebd-478c-ade4-39cd3ba9d0f1580e04d6-2496-45ed-86a2-aa0657b90282fa39877e-fcbe-4540-a735-8fedfc729bb8info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALFIGUEIRA, FERNANDA HERNANDES.pdfapplication/pdf809395http://repositorio.ufsm.br/bitstream/1/11225/1/FIGUEIRA%2c%20FERNANDA%20HERNANDES.pdf059979371d039b6d7d4d88717851b71dMD51TEXTFIGUEIRA, FERNANDA HERNANDES.pdf.txtFIGUEIRA, FERNANDA HERNANDES.pdf.txtExtracted texttext/plain72746http://repositorio.ufsm.br/bitstream/1/11225/2/FIGUEIRA%2c%20FERNANDA%20HERNANDES.pdf.txt08ef72831fe86f7441eeb29e18226b93MD52THUMBNAILFIGUEIRA, FERNANDA HERNANDES.pdf.jpgFIGUEIRA, FERNANDA HERNANDES.pdf.jpgIM Thumbnailimage/jpeg5448http://repositorio.ufsm.br/bitstream/1/11225/3/FIGUEIRA%2c%20FERNANDA%20HERNANDES.pdf.jpg2008849e64385621158018f8945f682fMD531/112252022-01-10 14:02:44.785oai:repositorio.ufsm.br:1/11225Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-01-10T17:02:44Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongos |
dc.title.alternative.eng.fl_str_mv |
Effect of diphenyl diselenide on behavioural and biochemical alterations induced by amphetamine in mice |
title |
Efeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongos |
spellingShingle |
Efeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongos Figueira, Fernanda Hernandes Disseleneto de difenila Monoamino oxidase Estereotipia Anfetamina Diphenyl diselenide Monoamine oxidase Stereotypy Amphetamine CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongos |
title_full |
Efeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongos |
title_fullStr |
Efeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongos |
title_full_unstemmed |
Efeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongos |
title_sort |
Efeito do disseleneto de difenila sobre alterações comportamentais e bioquímicas induzidas por anfetamina em camundongos |
author |
Figueira, Fernanda Hernandes |
author_facet |
Figueira, Fernanda Hernandes |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fachinetto, Roselei |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7203076675431306 |
dc.contributor.referee1.fl_str_mv |
Wagner, Caroline |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/4004565241849091 |
dc.contributor.referee2.fl_str_mv |
Pereira, Romaiana Picada |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3277439471060779 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7299721428462157 |
dc.contributor.author.fl_str_mv |
Figueira, Fernanda Hernandes |
contributor_str_mv |
Fachinetto, Roselei Wagner, Caroline Pereira, Romaiana Picada |
dc.subject.por.fl_str_mv |
Disseleneto de difenila Monoamino oxidase Estereotipia Anfetamina |
topic |
Disseleneto de difenila Monoamino oxidase Estereotipia Anfetamina Diphenyl diselenide Monoamine oxidase Stereotypy Amphetamine CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Diphenyl diselenide Monoamine oxidase Stereotypy Amphetamine |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Selenium is an element that can modulate the dopaminergic neurotransmission. Studies show that diphenyl diselenide, an organic compound of selenium, has antioxidant activity improves depressive-like behavior and reduce the activity of the enzyme monoamine oxidase (MAO). However, there are few studies concerning about possible alterations of diphenyl diselenide in dopaminergic system. Thus, the purpose of the present study was to evaluate the effects of acute and sub-chronic treatment of diphenyl diselenide on amphetamine-induced behavioral and biochemical alterations in mice. In the acute treatment, the mice were treated with diphenyl diselenide (5 and 10 mg/kg, s.c.) or vehicle (10% Tween 80, s.c.) 30 min before administration of amphetamine (1.25 mg/kg, i.p.). After 25 min, locomotor activity was assessed with an open field and, also, the time of stereotypy and immobility was assessed in a glass cage. Sub-chronic treatment was conducted with seven administrations of diphenyl diselenide (5 and 10 mg/kg, s.c.), or its vehicle being one administration per day. On the eighth day, amphetamine (1.25 mg/kg, i.p.) was administered and the behavioral tests were conducted after 25 min. In both treatments ex vivo tests were performed: isoform activity MAO-A and MAO-B, and measurement of total protein and non-protein thiol levels, oxidation of diclorofluorescein. Amphetamine increased the number of crossing and rearing in the open field test and diphenyl diselenide prevented only the increase in the number of crossings when acutely administered to mice. Furthermore, amphetamine increased the time of immobility and stereotypy in mice. Diphenyl diselenide did not prevent these effects. By contrary, at 10 mg/kg, sub-chronic administration of diphenyl diselenide increased per se the time of immobility and stereotypy. It was also found a positive correlation between immobility and stereotypy in acute and sub-chronic treatment with diphenyl diselenide. It was also detected a decrease in brain MAO-B activity caused by sub-chronic treatment with diphenyl diselenide either alone or in combination of amphetamine. Any change was detected in oxidative stress parameters. In conclusion, sub-chronic administration of diphenyl diselenide can promote a behavioral sensitization that seems to be, at least in part, dependent of MAO-B inhibition. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-09-19 |
dc.date.accessioned.fl_str_mv |
2015-02-26 |
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2015-02-26 |
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FIGUEIRA, Fernanda Hernandes. Effect of diphenyl diselenide on behavioural and biochemical alterations induced by amphetamine in mice. 2012. 54 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
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http://repositorio.ufsm.br/handle/1/11225 |
identifier_str_mv |
FIGUEIRA, Fernanda Hernandes. Effect of diphenyl diselenide on behavioural and biochemical alterations induced by amphetamine in mice. 2012. 54 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
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http://repositorio.ufsm.br/handle/1/11225 |
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Universidade Federal de Santa Maria |
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UFSM |
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Bioquímica |
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Universidade Federal de Santa Maria |
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