Biomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5614 |
Resumo: | The indiscriminate use of agricultural supplies can cause soil pollution, affecting the flora, fauna and ecological relationships. Among these chemical compounds used in agriculture, there is the Bordeaux mixture. The intense use of this fungicide results in a considerable copper addition in the soil, may cause damage to exposed organisms. This study aimed to evaluate the toxicological effects of different copper doses, (0, 35, 70, 105, 140 e 175 mg.Kg-1) applied in soil, at earthworms Eisenia andrei biomass and reproduction, through ecotoxicological tests as well as the effects of exposure to antioxidant enzyme activity, by the enzymes catalase and glutathione S-transferase (GST and CAT), on neurotransmission biomarkers such as acetylcholinesterase (AChE) and cell membrane lipid peroxidation through the levels of malondialdehyde (MDA). The ecotoxicological results showed that the largest copper doses tested, (140 e 175 mg.Kg-1) were toxic, because the worms had a mean biomass reduction and cocoons. In the enzyme assessments, an increase in the glutathione-S-transferase activity was observed as the antioxidant defense mechanism. The significant MDA levels increase and the acetylcholinesterase activity inhibition, indicating the cell membrane lipid peroxidation occurrence and neurotransmission changes as a result of oxidative stress caused by the metal. |
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2017-04-112017-04-112015-10-16FERREIRA, Talita. Biomarkers enzyme and ecotoxicity of copper in Eisenia andrei (Bouché 1972). 2015. 68 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/5614The indiscriminate use of agricultural supplies can cause soil pollution, affecting the flora, fauna and ecological relationships. Among these chemical compounds used in agriculture, there is the Bordeaux mixture. The intense use of this fungicide results in a considerable copper addition in the soil, may cause damage to exposed organisms. This study aimed to evaluate the toxicological effects of different copper doses, (0, 35, 70, 105, 140 e 175 mg.Kg-1) applied in soil, at earthworms Eisenia andrei biomass and reproduction, through ecotoxicological tests as well as the effects of exposure to antioxidant enzyme activity, by the enzymes catalase and glutathione S-transferase (GST and CAT), on neurotransmission biomarkers such as acetylcholinesterase (AChE) and cell membrane lipid peroxidation through the levels of malondialdehyde (MDA). The ecotoxicological results showed that the largest copper doses tested, (140 e 175 mg.Kg-1) were toxic, because the worms had a mean biomass reduction and cocoons. In the enzyme assessments, an increase in the glutathione-S-transferase activity was observed as the antioxidant defense mechanism. The significant MDA levels increase and the acetylcholinesterase activity inhibition, indicating the cell membrane lipid peroxidation occurrence and neurotransmission changes as a result of oxidative stress caused by the metal.O uso indiscriminado de insumos agrícolas pode causar a poluição dos solos, comprometendo a flora, fauna e as relações ecológicas. Dentre os insumos químicos utilizados na agricultura, destaca-se a calda bordalesa. Sua intensa utilização resulta em uma adição considerável de cobre ao solo, podendo causar danos aos organismos expostos. O presente estudo teve como objetivo determinar os efeitos toxicológicos de diferentes doses de cobre: 0, 35, 70, 105, 140 e 175 mg.Kg-1 aplicado ao solo, na biomassa e reprodução das minhocas Eisenia andrei, através dos testes ecotoxicológicos assim como os efeitos da exposição a nível enzimático antioxidante, através das enzimas catalase e glutationa S-transferase (CAT e GST), sobre biomarcadores de neurotransmissão como a acetil-colinesterase (AChE) e na peroxidação lipídica da membrana celular através dos níveis de malondialdeído (MDA). Os resultados ecotoxicológicos, evidenciaram que as maiores doses de cobre testadas, (140 e 175 mg.Kg-1) foram tóxicas, pois as minhocas apresentaram redução média na biomassa e no número de casulos. Em relação aos níveis enzimáticos, foi observado um incremento na atividade das enzimas glutationa-S-transferase, como mecanismo de defesa antioxidante. O aumento significativo nos níveis de MDA observados e a inibição da atividade da acetil-colinesterase, indicam a ocorrência da peroxidação lipídica da membrana celular e alterações na neurotransmissão em decorrência do estresse oxidativo ocasionado pela presença do metal.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciência do SoloUFSMBRAgronomiaEcotoxicologiaMetal pesadoMinhocaEnzimas antioxidantesEcotoxicologyHeavy metalEarthwormAntioxidant enzymeCNPQ::CIENCIAS AGRARIAS::AGRONOMIABiomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972)Biomarkers enzyme and ecotoxicity of copper in Eisenia andrei (Bouché 1972)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisAntoniolli, Zaida Ineshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787113T1Silveira, Andressa de Oliveirahttp://lattes.cnpq.br/1829365955344819Osorio Filho, Benjamin Diashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4771842H5http://lattes.cnpq.br/3936907102974161Ferreira, Talita5001000000094005003005005005e0cf37b-9daf-4778-a325-018d225a1d405f65ca1f-5665-4eb0-9abe-60b5db9d306e6ea6603a-15d9-481f-92e0-2d38f38f81547b944d97-bfbc-4f5a-8420-07dbcae46c96info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALFERREIRA, TALITA.pdfapplication/pdf963605http://repositorio.ufsm.br/bitstream/1/5614/1/FERREIRA%2c%20TALITA.pdf287de188a201bf1218c0a64f0a9c73abMD51TEXTFERREIRA, TALITA.pdf.txtFERREIRA, TALITA.pdf.txtExtracted texttext/plain125015http://repositorio.ufsm.br/bitstream/1/5614/2/FERREIRA%2c%20TALITA.pdf.txt5ee0ece2c90eb4bc60160d8ab20b3f1fMD52THUMBNAILFERREIRA, TALITA.pdf.jpgFERREIRA, TALITA.pdf.jpgIM Thumbnailimage/jpeg4711http://repositorio.ufsm.br/bitstream/1/5614/3/FERREIRA%2c%20TALITA.pdf.jpg4bb479dcccd7b1854b295893a91cea80MD531/56142021-01-18 08:05:58.474oai:repositorio.ufsm.br:1/5614Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132021-01-18T11:05:58Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Biomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972) |
dc.title.alternative.eng.fl_str_mv |
Biomarkers enzyme and ecotoxicity of copper in Eisenia andrei (Bouché 1972) |
title |
Biomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972) |
spellingShingle |
Biomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972) Ferreira, Talita Ecotoxicologia Metal pesado Minhoca Enzimas antioxidantes Ecotoxicology Heavy metal Earthworm Antioxidant enzyme CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Biomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972) |
title_full |
Biomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972) |
title_fullStr |
Biomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972) |
title_full_unstemmed |
Biomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972) |
title_sort |
Biomarcadores enzimáticos e ecotoxicidade por cobre em Eisenia andrei (Bouché 1972) |
author |
Ferreira, Talita |
author_facet |
Ferreira, Talita |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Antoniolli, Zaida Ines |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787113T1 |
dc.contributor.referee1.fl_str_mv |
Silveira, Andressa de Oliveira |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1829365955344819 |
dc.contributor.referee2.fl_str_mv |
Osorio Filho, Benjamin Dias |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4771842H5 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3936907102974161 |
dc.contributor.author.fl_str_mv |
Ferreira, Talita |
contributor_str_mv |
Antoniolli, Zaida Ines Silveira, Andressa de Oliveira Osorio Filho, Benjamin Dias |
dc.subject.por.fl_str_mv |
Ecotoxicologia Metal pesado Minhoca Enzimas antioxidantes |
topic |
Ecotoxicologia Metal pesado Minhoca Enzimas antioxidantes Ecotoxicology Heavy metal Earthworm Antioxidant enzyme CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Ecotoxicology Heavy metal Earthworm Antioxidant enzyme |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
The indiscriminate use of agricultural supplies can cause soil pollution, affecting the flora, fauna and ecological relationships. Among these chemical compounds used in agriculture, there is the Bordeaux mixture. The intense use of this fungicide results in a considerable copper addition in the soil, may cause damage to exposed organisms. This study aimed to evaluate the toxicological effects of different copper doses, (0, 35, 70, 105, 140 e 175 mg.Kg-1) applied in soil, at earthworms Eisenia andrei biomass and reproduction, through ecotoxicological tests as well as the effects of exposure to antioxidant enzyme activity, by the enzymes catalase and glutathione S-transferase (GST and CAT), on neurotransmission biomarkers such as acetylcholinesterase (AChE) and cell membrane lipid peroxidation through the levels of malondialdehyde (MDA). The ecotoxicological results showed that the largest copper doses tested, (140 e 175 mg.Kg-1) were toxic, because the worms had a mean biomass reduction and cocoons. In the enzyme assessments, an increase in the glutathione-S-transferase activity was observed as the antioxidant defense mechanism. The significant MDA levels increase and the acetylcholinesterase activity inhibition, indicating the cell membrane lipid peroxidation occurrence and neurotransmission changes as a result of oxidative stress caused by the metal. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-10-16 |
dc.date.accessioned.fl_str_mv |
2017-04-11 |
dc.date.available.fl_str_mv |
2017-04-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERREIRA, Talita. Biomarkers enzyme and ecotoxicity of copper in Eisenia andrei (Bouché 1972). 2015. 68 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5614 |
identifier_str_mv |
FERREIRA, Talita. Biomarkers enzyme and ecotoxicity of copper in Eisenia andrei (Bouché 1972). 2015. 68 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
url |
http://repositorio.ufsm.br/handle/1/5614 |
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por |
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Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Ciência do Solo |
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UFSM |
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Agronomia |
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Universidade Federal de Santa Maria |
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