Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiá

Detalhes bibliográficos
Autor(a) principal: Almeida, Ana Paula Gottlieb
Data de Publicação: 2018
Tipo de documento: Tese
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
dARK ID: ark:/26339/001300000h32c
Texto Completo: http://repositorio.ufsm.br/handle/1/16299
Resumo: Anesthetics of synthetic origin are widely used in aquaculture to minimize animal stress arising from the practices involved in this activity. However, the adverse effects caused by these synthetic substances have encouraged research for natural substances with sedative and anesthetics properties that are more effective, safer and with lower risk of adverse effects. Studies have shown that essential oils (EOs) extracted from the plants species Aloysia triphylla and Lippia alba, as well as eugenol, an isolated compound of clove oil, present sedative and anesthetic properties in fish, being considered promising alternatives to traditional anesthetics. In this context, the first part of this study aimed to investigate the effects of these EOs on two Amazonian species, Serrasalmus rhombeus and S. eigenmanni, inhabitantsof Rio Negro. In the second part, we evaluated the gene expression of clock and digestive enzymes genes in post-larvae and juvenile of Rhamdia quelen. In the article 1, the sedative and anesthetic efficacy of A. triphylla and L. alba EOs and eugenol and the recovery time was determined in Serrasalmus rhombeus, as well as the swimming behavior on a short exposure of 15 min at low concentrations to evaluate the possible use of these substances in the transport of this species. Both EOs and eugenol are effective, with concentrations of 150, 200, and 50 μL L-1, respectively, being recommended for anestesia. For sedation, it is recommended to use 50 μL L-1 for both EOs and 25 μL L-1 for eugenol. The concentrations of 5 and 10 μL L-1 are indicated for transport studies with these anesthetics. In manuscript 2, the sedative and anesthetic efficacy of A. triphylla and L. alba EOs in Serrasalmus eigenmanni, as well as recovery time of the fish were determined. The net ion fluxes, blood parameters, and swimming behavior at a prolonged exposure of 4 h were also evaluated. Both EOs were effective for anesthesia of S. eigenmanni and the concentration of 100 μL L-1 was recommended for both EOs. For sedation, the indicated concentrations are 25 and 50 μL L-1 of A. triphylla and L. alba EOs, respectively. For transport, the concentration of 5 μL L-1 of A. triphylla EO is recommended due to the lower ammonia excretion and maintenance of fish equilibrium. In post-larvae, genes of the positive loop (artnl1a and clock) and the negative loop (per1,2,3 and cry1,2) presented differences in expression levels throughout the evaluated period, with acrophase occurring in the same schedule. In juveniles, only one positive loop gene (arntl1) and one negative loop gene (cry2) showed differences in expression levels in the evaluated period, with a difference of 1 h between the acrophases. The levels of gene expression of the digestive enzymes in post-larvae were maximal 4 h before feeding and at least 1 h after feeding. In the digestive tract of juveniles the levels of gene expression of the digestive enzymes did not differ in the evaluated period. In conclusion, both OEs are effective for sedation and anesthesia of S. rhombeus and S. eigenmanni, and may also be used in transport studies for these species. Both post-larvae and juveniles did not exhibit the typical interaction between the genes of the positive (arntl and clock) and negative (per and cry) loops and only in post-larvae the gene expression of the digestive enzymes is probably influenced by the genes clock.
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spelling Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiáEssential oils as anesthetics in serrasalmds and gene expression of clock genes and digestive enzymes in silver catfishAloysia thiphyllaAnestesiaCiclo circadianoLippia albamRNARitmo alimentarAnesthesiaCircadian cycleFeedingCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAnesthetics of synthetic origin are widely used in aquaculture to minimize animal stress arising from the practices involved in this activity. However, the adverse effects caused by these synthetic substances have encouraged research for natural substances with sedative and anesthetics properties that are more effective, safer and with lower risk of adverse effects. Studies have shown that essential oils (EOs) extracted from the plants species Aloysia triphylla and Lippia alba, as well as eugenol, an isolated compound of clove oil, present sedative and anesthetic properties in fish, being considered promising alternatives to traditional anesthetics. In this context, the first part of this study aimed to investigate the effects of these EOs on two Amazonian species, Serrasalmus rhombeus and S. eigenmanni, inhabitantsof Rio Negro. In the second part, we evaluated the gene expression of clock and digestive enzymes genes in post-larvae and juvenile of Rhamdia quelen. In the article 1, the sedative and anesthetic efficacy of A. triphylla and L. alba EOs and eugenol and the recovery time was determined in Serrasalmus rhombeus, as well as the swimming behavior on a short exposure of 15 min at low concentrations to evaluate the possible use of these substances in the transport of this species. Both EOs and eugenol are effective, with concentrations of 150, 200, and 50 μL L-1, respectively, being recommended for anestesia. For sedation, it is recommended to use 50 μL L-1 for both EOs and 25 μL L-1 for eugenol. The concentrations of 5 and 10 μL L-1 are indicated for transport studies with these anesthetics. In manuscript 2, the sedative and anesthetic efficacy of A. triphylla and L. alba EOs in Serrasalmus eigenmanni, as well as recovery time of the fish were determined. The net ion fluxes, blood parameters, and swimming behavior at a prolonged exposure of 4 h were also evaluated. Both EOs were effective for anesthesia of S. eigenmanni and the concentration of 100 μL L-1 was recommended for both EOs. For sedation, the indicated concentrations are 25 and 50 μL L-1 of A. triphylla and L. alba EOs, respectively. For transport, the concentration of 5 μL L-1 of A. triphylla EO is recommended due to the lower ammonia excretion and maintenance of fish equilibrium. In post-larvae, genes of the positive loop (artnl1a and clock) and the negative loop (per1,2,3 and cry1,2) presented differences in expression levels throughout the evaluated period, with acrophase occurring in the same schedule. In juveniles, only one positive loop gene (arntl1) and one negative loop gene (cry2) showed differences in expression levels in the evaluated period, with a difference of 1 h between the acrophases. The levels of gene expression of the digestive enzymes in post-larvae were maximal 4 h before feeding and at least 1 h after feeding. In the digestive tract of juveniles the levels of gene expression of the digestive enzymes did not differ in the evaluated period. In conclusion, both OEs are effective for sedation and anesthesia of S. rhombeus and S. eigenmanni, and may also be used in transport studies for these species. Both post-larvae and juveniles did not exhibit the typical interaction between the genes of the positive (arntl and clock) and negative (per and cry) loops and only in post-larvae the gene expression of the digestive enzymes is probably influenced by the genes clock.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESAnestésicos de origem sintética são amplamente empregados na aquicultura para minimizar o estresse decorrente das práticas envolvidas nessa atividade. No entanto, os efeitos adversos causados por essas substâncias sintéticas têm incentivado a pesquisa por alternativas naturais com propriedades sedativas e anestésicas que apresentem maior eficácia, segurança e menor risco de efeitos adversos. Estudos comprovaram que os óleos essenciais (OEs) extraídos das espécies vegetais Aloysia triphylla e Lippia alba, bem como o eugenol, um composto isolado do OE de cravo, apresentam propriedades sedativas e anestésicas em peixes, sendo considerados, portanto, alternativas promissoras aos anestésicos tradicionais. Nesse contexto, a primeira parte deste estudo objetivou investigar os efeitos desses OEs em duas espécies amazônicas, Serrasalmus rhombeus e S. eigenmanni, habitantes do Rio Negro. Já na segunda parte, avaliou-se a expressão gênica de genes relógio e de enzimas digestivas em pós-larvas e juvenis de Rhamdia quelen. O artigo 1 determinou a eficácia sedativa e anestésica dos OEs de A. triphylla e de L. alba e do eugenol em S. rhombeus e o tempo de recuperação dos animais, bem como comportamento natatório em uma exposição curta de 15 min em concentrações baixas para avaliar o possível uso dessas substâncias no transporte dessa espécie. Ambos OEs e o eugenol são eficazes, sendo recomendadas as concentrações de 150, 200 e 50 μL L-1, respectivamente, para anestesia. Para a sedação dessa espécie é recomendada a utilização de 50 μL L-1 para ambos OEs e 25 μL L-1 para o eugenol. As concentrações de 5 e 10 μL L-1 são indicadas para estudos de transporte com esses anestésicos. No manuscrito 2 foi determinada a eficácia sedativa e anestésica dos OEs de A. triphylla e de L. alba em S. eigenmanni, bem como o tempo de recuperação dos animais. Avaliou-se também o fluxo iônico líquido, parâmetros sanguíneos e o comportamento natatório em uma exposição prolongada de 4 h. Ambos OEs foram eficazes para anestesia de S. eigenmanni, sendo recomendada a concentração de 100 μL L-1 para ambos os OEs e para a sedação as concentrações indicadas são 25 e 50 μL L-1 de OEs de A. triphylla e de L. alba, respectivamente. Para o transporte sugere-se a concentração de 5 μL L-1 do OE de A. triphylla devido à diminuição da excreção de amônia e à manutenção do equilíbrio. No manuscrito 3, foi avaliado a expressão gênica de genes relógio e de enzimas digestivas em pós-larvas e juvenis de R. quelen. Em pós-larvas, alguns genes da alça positiva (artnl1a e clock) e da alça negativa (per1,2,3 e cry1,2) apresentaram diferenças nos níveis de expressão ao longo do período avaliado, sendo que a acrofase ocorreu no mesmo horário. Em juvenis, um gene da alça positiva (arntl1l) e um gene da alça negativa (cry2) apresentaram diferenças nos níveis de expressão, com diferença de 1 h entre as acrófases. Os níveis de expressão gênica das enzimas digestivas em pós-larvas foram máximos 4 h antes da alimentação e mínimos 1 h após a alimentação. No trato gastrintestinal de juvenis os níveis de expressão gênica das enzimas digestivas não diferiu no período avaliado. Em conclusão, ambos os OEs são eficazes para sedação e anestesia de S. rhombeus e de S. eigenmanni, podendo também serem utilizados em estudos de transportes para essas espécies. Tanto as pós-larvas quanto os juvenis não exibiram a interação típica entre os genes das alças positiva (arntl e clock) e negativa (per e cry) e somente em pós-larvas a expressão gênica das enzimas digestivas é, provavelmente, influenciada pelos genes relógio.Universidade Federal de Santa MariaBrasilBioquímicaUFSMPrograma de Pós-Graduação em Biodiversidade AnimalCentro de Ciências Naturais e ExatasBaldisserotto, Bernardohttp://lattes.cnpq.br/1036046601275319Pavanato, Maria Amáliahttp://lattes.cnpq.br/8701892865724171Behr, Everton Rodolfohttp://lattes.cnpq.br/5793290654126265Toni, Cândidahttp://lattes.cnpq.br/3577140585919842Becker, Alexssandro Gefersonhttp://lattes.cnpq.br/2447793240708317Almeida, Ana Paula Gottlieb2019-04-23T21:01:39Z2019-04-23T21:01:39Z2018-08-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/16299ark:/26339/001300000h32cporAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2019-04-24T06:01:55Zoai:repositorio.ufsm.br:1/16299Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2024-07-29T10:39:45.394054Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiá
Essential oils as anesthetics in serrasalmds and gene expression of clock genes and digestive enzymes in silver catfish
title Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiá
spellingShingle Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiá
Almeida, Ana Paula Gottlieb
Aloysia thiphylla
Anestesia
Ciclo circadiano
Lippia alba
mRNA
Ritmo alimentar
Anesthesia
Circadian cycle
Feeding
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
title_short Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiá
title_full Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiá
title_fullStr Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiá
title_full_unstemmed Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiá
title_sort Óleos essenciais como anestésicos em serrasalmídeos e expressão gênica de genes relógio e das enzimas digestivas em jundiá
author Almeida, Ana Paula Gottlieb
author_facet Almeida, Ana Paula Gottlieb
author_role author
dc.contributor.none.fl_str_mv Baldisserotto, Bernardo
http://lattes.cnpq.br/1036046601275319
Pavanato, Maria Amália
http://lattes.cnpq.br/8701892865724171
Behr, Everton Rodolfo
http://lattes.cnpq.br/5793290654126265
Toni, Cândida
http://lattes.cnpq.br/3577140585919842
Becker, Alexssandro Geferson
http://lattes.cnpq.br/2447793240708317
dc.contributor.author.fl_str_mv Almeida, Ana Paula Gottlieb
dc.subject.por.fl_str_mv Aloysia thiphylla
Anestesia
Ciclo circadiano
Lippia alba
mRNA
Ritmo alimentar
Anesthesia
Circadian cycle
Feeding
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
topic Aloysia thiphylla
Anestesia
Ciclo circadiano
Lippia alba
mRNA
Ritmo alimentar
Anesthesia
Circadian cycle
Feeding
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA
description Anesthetics of synthetic origin are widely used in aquaculture to minimize animal stress arising from the practices involved in this activity. However, the adverse effects caused by these synthetic substances have encouraged research for natural substances with sedative and anesthetics properties that are more effective, safer and with lower risk of adverse effects. Studies have shown that essential oils (EOs) extracted from the plants species Aloysia triphylla and Lippia alba, as well as eugenol, an isolated compound of clove oil, present sedative and anesthetic properties in fish, being considered promising alternatives to traditional anesthetics. In this context, the first part of this study aimed to investigate the effects of these EOs on two Amazonian species, Serrasalmus rhombeus and S. eigenmanni, inhabitantsof Rio Negro. In the second part, we evaluated the gene expression of clock and digestive enzymes genes in post-larvae and juvenile of Rhamdia quelen. In the article 1, the sedative and anesthetic efficacy of A. triphylla and L. alba EOs and eugenol and the recovery time was determined in Serrasalmus rhombeus, as well as the swimming behavior on a short exposure of 15 min at low concentrations to evaluate the possible use of these substances in the transport of this species. Both EOs and eugenol are effective, with concentrations of 150, 200, and 50 μL L-1, respectively, being recommended for anestesia. For sedation, it is recommended to use 50 μL L-1 for both EOs and 25 μL L-1 for eugenol. The concentrations of 5 and 10 μL L-1 are indicated for transport studies with these anesthetics. In manuscript 2, the sedative and anesthetic efficacy of A. triphylla and L. alba EOs in Serrasalmus eigenmanni, as well as recovery time of the fish were determined. The net ion fluxes, blood parameters, and swimming behavior at a prolonged exposure of 4 h were also evaluated. Both EOs were effective for anesthesia of S. eigenmanni and the concentration of 100 μL L-1 was recommended for both EOs. For sedation, the indicated concentrations are 25 and 50 μL L-1 of A. triphylla and L. alba EOs, respectively. For transport, the concentration of 5 μL L-1 of A. triphylla EO is recommended due to the lower ammonia excretion and maintenance of fish equilibrium. In post-larvae, genes of the positive loop (artnl1a and clock) and the negative loop (per1,2,3 and cry1,2) presented differences in expression levels throughout the evaluated period, with acrophase occurring in the same schedule. In juveniles, only one positive loop gene (arntl1) and one negative loop gene (cry2) showed differences in expression levels in the evaluated period, with a difference of 1 h between the acrophases. The levels of gene expression of the digestive enzymes in post-larvae were maximal 4 h before feeding and at least 1 h after feeding. In the digestive tract of juveniles the levels of gene expression of the digestive enzymes did not differ in the evaluated period. In conclusion, both OEs are effective for sedation and anesthesia of S. rhombeus and S. eigenmanni, and may also be used in transport studies for these species. Both post-larvae and juveniles did not exhibit the typical interaction between the genes of the positive (arntl and clock) and negative (per and cry) loops and only in post-larvae the gene expression of the digestive enzymes is probably influenced by the genes clock.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-31
2019-04-23T21:01:39Z
2019-04-23T21:01:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/16299
dc.identifier.dark.fl_str_mv ark:/26339/001300000h32c
url http://repositorio.ufsm.br/handle/1/16299
identifier_str_mv ark:/26339/001300000h32c
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Bioquímica
UFSM
Programa de Pós-Graduação em Biodiversidade Animal
Centro de Ciências Naturais e Exatas
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Bioquímica
UFSM
Programa de Pós-Graduação em Biodiversidade Animal
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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