Investigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculas

Detalhes bibliográficos
Autor(a) principal: Menezes, Lucas Brandalise
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional Manancial UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/19554
Resumo: The objective of this work was to investigate the photophysical properties of two porphyrins whose syntheses are already reported in the literature: meso-tetra(4-pyridyl)porphyrin tetra-ruthenated free-base (H2RuTPyP) and meso-tetra(4-pyridyl)porphyrin tetra-ruthenated zinc(II) (ZnRuTPyP). For this, both porphyrins were synthesized according to the Araki and co-workers methodology and properly characterized by elemental analysis, mass spectrometry, ultraviolet-visible, infrared and emission spectroscopy. In addition, solution aggregation assays were performed, photostability studies and singlet oxygen generation were investigated by UV-vis spectroscopy. Moreover, the interactive studies with DNA and HSA were performed via UV-vis spectroscopy, fluorescence emission and theoretical calculations by molecular docking. ZnII-porphyrin (ZnRuTPyP) presented better values for intrinsic DNA binding constant (Kb), and Stern-Volmer quenching constant (KSV), suggesting that both tetra-ruthenated porphyrins interact with major groove and are governed by electrostatic interactions, which is evidenced by molecular docking calculations.
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spelling 2020-02-12T12:16:16Z2020-02-12T12:16:16Z2019-08-05http://repositorio.ufsm.br/handle/1/19554The objective of this work was to investigate the photophysical properties of two porphyrins whose syntheses are already reported in the literature: meso-tetra(4-pyridyl)porphyrin tetra-ruthenated free-base (H2RuTPyP) and meso-tetra(4-pyridyl)porphyrin tetra-ruthenated zinc(II) (ZnRuTPyP). For this, both porphyrins were synthesized according to the Araki and co-workers methodology and properly characterized by elemental analysis, mass spectrometry, ultraviolet-visible, infrared and emission spectroscopy. In addition, solution aggregation assays were performed, photostability studies and singlet oxygen generation were investigated by UV-vis spectroscopy. Moreover, the interactive studies with DNA and HSA were performed via UV-vis spectroscopy, fluorescence emission and theoretical calculations by molecular docking. ZnII-porphyrin (ZnRuTPyP) presented better values for intrinsic DNA binding constant (Kb), and Stern-Volmer quenching constant (KSV), suggesting that both tetra-ruthenated porphyrins interact with major groove and are governed by electrostatic interactions, which is evidenced by molecular docking calculations.Este trabalho teve como objetivo investigar as propriedades fotofísicas de duas porfirinas cujas sínteses já são relatas na literatura: meso-tetra(4-piridil)porfirina base-livre tetra-rutenada (H2RuTPyP) e meso-tetra(4-piridil)porfirina de zinco(II) tetra-rutenada (ZnRuTPyP). Para isso, ambas foram sintetizadas de acordo com a metodologia relatada Araki e colaboradores e devidamente caracterizadas por análise elementar, espectrometria de massas, espectroscopia eletrônica na região do ultravioleta-visível, espectroscopia vibracional na região do infravermelho e espectroscopia de emissão. Além disso, foram realizados ensaios de agregação em solução, estudos de fotoestabilidade e geração de oxigênio singleto foram investigadas por espectroscopia na região do UV-vis. Mais ainda, os estudos de interação com DNA e HSA foram realizados via espectroscopia UV-vis, emissão fluorescente e de cálculos teóricos por docking molecular. A porfirina de ZnII ZnRuTPyP apresentou melhores valores para constante intrínseca de ligação ao DNA (Kb), e para valores de supressão de Stern-Volmer (KSV), sugerindo que ambas as porfirinas interagem via sulco maior (major groove) e regidas por interações eletrostáticas, o que é evidenciado pelos cálculos de docking molecular.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPorfirinas de rutênio(II)FotofísicaInteração com DNAInteração com HSARuthenium(II) porphyrinsPhotophysicsDNA interactionsHSA interactionsCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAInvestigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculasInvestigation of the photophysical properties of meso-substituted porphyrins with RuII complexes and study of interaction with biomoleculesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisIglesias, Bernardo Almeidahttp://lattes.cnpq.br/4402375533322977Bonacin, Juliano Alveshttp://lattes.cnpq.br/9730622542566256Burgo, Thiago Augusto de Limahttp://lattes.cnpq.br/5737213839553155http://lattes.cnpq.br/5916616069384509Menezes, Lucas Brandalise1006000000006003140bdfe-ad7b-478b-8ebd-065c1627c132246864a1-9d48-4ec6-8b5f-ba2d00c38772c6b56aa6-1100-498b-958a-2509f1d7d1f8ea58280d-c311-4e40-af49-8adb2aeee147reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGQUIMICA_2019_MENEZES_LUCAS.pdfDIS_PPGQUIMICA_2019_MENEZES_LUCAS.pdfDissertação de Mestradoapplication/pdf4349212http://repositorio.ufsm.br/bitstream/1/19554/1/DIS_PPGQUIMICA_2019_MENEZES_LUCAS.pdff2883bda56a667bec18e1845f764b528MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv Investigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculas
dc.title.alternative.eng.fl_str_mv Investigation of the photophysical properties of meso-substituted porphyrins with RuII complexes and study of interaction with biomolecules
title Investigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculas
spellingShingle Investigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculas
Menezes, Lucas Brandalise
Porfirinas de rutênio(II)
Fotofísica
Interação com DNA
Interação com HSA
Ruthenium(II) porphyrins
Photophysics
DNA interactions
HSA interactions
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Investigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculas
title_full Investigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculas
title_fullStr Investigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculas
title_full_unstemmed Investigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculas
title_sort Investigação das propriedades fotofísicas de porfirinas meso substituídas com complexos de RuII e estudo da interação com biomoléculas
author Menezes, Lucas Brandalise
author_facet Menezes, Lucas Brandalise
author_role author
dc.contributor.advisor1.fl_str_mv Iglesias, Bernardo Almeida
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4402375533322977
dc.contributor.referee1.fl_str_mv Bonacin, Juliano Alves
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/9730622542566256
dc.contributor.referee2.fl_str_mv Burgo, Thiago Augusto de Lima
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/5737213839553155
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/5916616069384509
dc.contributor.author.fl_str_mv Menezes, Lucas Brandalise
contributor_str_mv Iglesias, Bernardo Almeida
Bonacin, Juliano Alves
Burgo, Thiago Augusto de Lima
dc.subject.por.fl_str_mv Porfirinas de rutênio(II)
Fotofísica
Interação com DNA
Interação com HSA
topic Porfirinas de rutênio(II)
Fotofísica
Interação com DNA
Interação com HSA
Ruthenium(II) porphyrins
Photophysics
DNA interactions
HSA interactions
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Ruthenium(II) porphyrins
Photophysics
DNA interactions
HSA interactions
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
description The objective of this work was to investigate the photophysical properties of two porphyrins whose syntheses are already reported in the literature: meso-tetra(4-pyridyl)porphyrin tetra-ruthenated free-base (H2RuTPyP) and meso-tetra(4-pyridyl)porphyrin tetra-ruthenated zinc(II) (ZnRuTPyP). For this, both porphyrins were synthesized according to the Araki and co-workers methodology and properly characterized by elemental analysis, mass spectrometry, ultraviolet-visible, infrared and emission spectroscopy. In addition, solution aggregation assays were performed, photostability studies and singlet oxygen generation were investigated by UV-vis spectroscopy. Moreover, the interactive studies with DNA and HSA were performed via UV-vis spectroscopy, fluorescence emission and theoretical calculations by molecular docking. ZnII-porphyrin (ZnRuTPyP) presented better values for intrinsic DNA binding constant (Kb), and Stern-Volmer quenching constant (KSV), suggesting that both tetra-ruthenated porphyrins interact with major groove and are governed by electrostatic interactions, which is evidenced by molecular docking calculations.
publishDate 2019
dc.date.issued.fl_str_mv 2019-08-05
dc.date.accessioned.fl_str_mv 2020-02-12T12:16:16Z
dc.date.available.fl_str_mv 2020-02-12T12:16:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/19554
url http://repositorio.ufsm.br/handle/1/19554
dc.language.iso.fl_str_mv por
language por
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dc.relation.confidence.fl_str_mv 600
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dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Química
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Química
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Repositório Institucional Manancial UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Repositório Institucional Manancial UFSM
collection Repositório Institucional Manancial UFSM
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