Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio)
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/9009 |
Resumo: | The presence of pharmaceuticals in the aquatic environment has long been reported for some years in various studies. However, the impact of drugs on organisms present in aquatic ecosystems is still poorly known. Thus, we investigated the effects of acute exposure to diazepam or fluoxetine on the response to acute stress in zebrafish (Danio rerio). Fish were exposed to drugs for 15 minutes and, after this period, the group designated time 0 was collected; the other groups were subjected to a stimulus of stress, which consisted in persecution of fish with a net for two minutes, and after that, were collected at the times of 15, 60 and 240 minutes. Three concentrations of diazepam 0.88 μg/L (environmental concentration) were used; 16 μg/L; and 160 μg/L, concentrations that cause behavioral effects in zebrafish. Regarding fluoxetine at concentrations of 1 μg/L, 25 μg/L and 50 μg/L (25 to 50 times the concentration of the environment, respectively) were used. Our results show that diazepam and fluoxetine inhibit the axis of stress in the zebrafish. The intermediate concentration of diazepam is able to suppress the stress response, as measured by levels of cortisol whereas the fluoxetine is able to inhibit the stress response at concentrations similar to those found in the environment. Fish with an impaired stress response loses the ability to maintain homeostasis against stress factors, since it reduces the ability to promote ionic, metabolic and behavioral adjustments necessary for such response. Thus, these data suggest that the presence of psychoactive drugs in aquatic ecosystems may cause neuroendocrine dysfunction in fish. |
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Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio)Diazepam and fluoxetine inhibit the stress response in zebrafish (Danio rerio)DiazepamFluoxetinaPsicofármacosEstresseZebrafishDanio rerioDiazepamFluoxetinePsychotropicsStressZebrafishCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAThe presence of pharmaceuticals in the aquatic environment has long been reported for some years in various studies. However, the impact of drugs on organisms present in aquatic ecosystems is still poorly known. Thus, we investigated the effects of acute exposure to diazepam or fluoxetine on the response to acute stress in zebrafish (Danio rerio). Fish were exposed to drugs for 15 minutes and, after this period, the group designated time 0 was collected; the other groups were subjected to a stimulus of stress, which consisted in persecution of fish with a net for two minutes, and after that, were collected at the times of 15, 60 and 240 minutes. Three concentrations of diazepam 0.88 μg/L (environmental concentration) were used; 16 μg/L; and 160 μg/L, concentrations that cause behavioral effects in zebrafish. Regarding fluoxetine at concentrations of 1 μg/L, 25 μg/L and 50 μg/L (25 to 50 times the concentration of the environment, respectively) were used. Our results show that diazepam and fluoxetine inhibit the axis of stress in the zebrafish. The intermediate concentration of diazepam is able to suppress the stress response, as measured by levels of cortisol whereas the fluoxetine is able to inhibit the stress response at concentrations similar to those found in the environment. Fish with an impaired stress response loses the ability to maintain homeostasis against stress factors, since it reduces the ability to promote ionic, metabolic and behavioral adjustments necessary for such response. Thus, these data suggest that the presence of psychoactive drugs in aquatic ecosystems may cause neuroendocrine dysfunction in fish.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorA presença de produtos farmacêuticos no ambiente aquático tem sido relatada há alguns anos em vários estudos. No entanto, o impacto de medicamentos sobre os organismos presentes nesses ecossistemas ainda é pouco conhecido. Assim, investigamos os efeitos da exposição aguda ao diazepam e à fluoxetina sobre a resposta ao estresse agudo em zebrafish (Danio rerio). Os peixes foram expostos aos fármacos por 15 minutos e, após esse período, o grupo denominado tempo 0 foi coletado; os demais grupos foram submetidos a um estímulo de estresse, com perseguição dos peixes com uma rede durante dois minutos, e, após, foram coletados nos tempos de 15, 60 e 240 minutos. Foram utilizadas três concentrações de diazepam a 0,88 μg/L (concentração ambiental); 16 μg/L; e 160 μg/L, concentração com efeitos comportamentais relatados em zebrafish. Em relação à fluoxetina, foram utilizadas as concentrações de 1 μg/L, 25 μg/L e 50 μg/L (25 a 50 vezes a concentração do ambiente, respectivamente). Nossos resultados demonstram que o diazepam e a fluoxetina inibem o eixo de estresse no zebrafish. A concentração intermediária de diazepam é capaz de suprimir a resposta de estresse, conforme se mediu pelos níveis de cortisol, ao passo que a fluoxetina é capaz de inibir a resposta ao estresse em concentrações semelhantes às encontradas no ambiente. Peixes com resposta de estresse prejudicada perdem a capacidade de manter a homeostase contra fatores de estresse, uma vez que reduz a capacidade de promover ajustes iônicos, metabólicos e comportamentais necessários para tal resposta. Assim, esses dados sugerem que a presença de fármacos psicoativos em ecossistemas aquáticos pode causar disfunção neuroendócrina em peixes.Universidade Federal de Santa MariaBRFarmacologiaUFSMPrograma de Pós-Graduação em FarmacologiaBarcellos, Leonardo Jose Gilhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763175J2Bonan, Carla Denisehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799528J3Kreutz, Luiz Carloshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784753P1Abreu, Murilo Sander de2015-03-192015-03-192014-08-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfABREU, Murilo Sander de. DIAZEPAM AND FLUOXETINE INHIBIT THE STRESS RESPONSE IN ZEBRAFISH (Danio rerio). 2014. 36 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/9009porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2021-02-04T12:23:35Zoai:repositorio.ufsm.br:1/9009Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2021-02-04T12:23:35Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio) Diazepam and fluoxetine inhibit the stress response in zebrafish (Danio rerio) |
title |
Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio) |
spellingShingle |
Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio) Abreu, Murilo Sander de Diazepam Fluoxetina Psicofármacos Estresse Zebrafish Danio rerio Diazepam Fluoxetine Psychotropics Stress Zebrafish CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio) |
title_full |
Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio) |
title_fullStr |
Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio) |
title_full_unstemmed |
Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio) |
title_sort |
Diazepam e fluoxetina inibem a resposta de estresse em zebrafish (Danio rerio) |
author |
Abreu, Murilo Sander de |
author_facet |
Abreu, Murilo Sander de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Barcellos, Leonardo Jose Gil http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763175J2 Bonan, Carla Denise http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799528J3 Kreutz, Luiz Carlos http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784753P1 |
dc.contributor.author.fl_str_mv |
Abreu, Murilo Sander de |
dc.subject.por.fl_str_mv |
Diazepam Fluoxetina Psicofármacos Estresse Zebrafish Danio rerio Diazepam Fluoxetine Psychotropics Stress Zebrafish CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
topic |
Diazepam Fluoxetina Psicofármacos Estresse Zebrafish Danio rerio Diazepam Fluoxetine Psychotropics Stress Zebrafish CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
The presence of pharmaceuticals in the aquatic environment has long been reported for some years in various studies. However, the impact of drugs on organisms present in aquatic ecosystems is still poorly known. Thus, we investigated the effects of acute exposure to diazepam or fluoxetine on the response to acute stress in zebrafish (Danio rerio). Fish were exposed to drugs for 15 minutes and, after this period, the group designated time 0 was collected; the other groups were subjected to a stimulus of stress, which consisted in persecution of fish with a net for two minutes, and after that, were collected at the times of 15, 60 and 240 minutes. Three concentrations of diazepam 0.88 μg/L (environmental concentration) were used; 16 μg/L; and 160 μg/L, concentrations that cause behavioral effects in zebrafish. Regarding fluoxetine at concentrations of 1 μg/L, 25 μg/L and 50 μg/L (25 to 50 times the concentration of the environment, respectively) were used. Our results show that diazepam and fluoxetine inhibit the axis of stress in the zebrafish. The intermediate concentration of diazepam is able to suppress the stress response, as measured by levels of cortisol whereas the fluoxetine is able to inhibit the stress response at concentrations similar to those found in the environment. Fish with an impaired stress response loses the ability to maintain homeostasis against stress factors, since it reduces the ability to promote ionic, metabolic and behavioral adjustments necessary for such response. Thus, these data suggest that the presence of psychoactive drugs in aquatic ecosystems may cause neuroendocrine dysfunction in fish. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-27 2015-03-19 2015-03-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ABREU, Murilo Sander de. DIAZEPAM AND FLUOXETINE INHIBIT THE STRESS RESPONSE IN ZEBRAFISH (Danio rerio). 2014. 36 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2014. http://repositorio.ufsm.br/handle/1/9009 |
identifier_str_mv |
ABREU, Murilo Sander de. DIAZEPAM AND FLUOXETINE INHIBIT THE STRESS RESPONSE IN ZEBRAFISH (Danio rerio). 2014. 36 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
url |
http://repositorio.ufsm.br/handle/1/9009 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmacologia UFSM Programa de Pós-Graduação em Farmacologia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Farmacologia UFSM Programa de Pós-Graduação em Farmacologia |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
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1805922098311331840 |