Composição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/9001 |
Resumo: | This work was designed to isolate the active compounds responsible for the central activity detected for the essential oil of Aloysia gratissima (EOA) in silver catfish (Rhamdia quelen). The AChE (acetylcholiesterase) activity under the influence of EOA was also investigated. Silver catfish were submitted to anesthesia bath with EOA (90, 300, 450 or 900 mg L-1), their fractions and isolated compounds to determine time of anesthetic induction and recovery. Eugenol (50 mg L-1) was used as positive control. EOA was effective as anesthetic at concentrations of 300 to 900 mg L-1. However, silver catfish presented involuntary muscle contractions during induction and recovery. AChE activity was not inhibited by EOA. The bio-guided fractionation of EOA furnished E-(-)-pinocamphone, (-)-caryophyllene oxide, (-)- guaiol and (+)-spathulenol, which were identified by mass spectrometry (MS), 1H- and 13CNMR. E-(-)-pinocamphone caused the same side effects observed to the EOA. (-)- Caryophyllene oxide, (-)-guaiol and (+)-spathulenol showed only sedative effects at concentrations proportional to those of the constituents in EOA. (-)-Caryophyllene oxide was also sedative in higher concentration (40 mg L-1) than present in EOA while (+)-spathulenol (51.2 mg L-1) promoted deep anesthesia without side effects. EOA showed anesthetic properties, however, should be used with caution in silver catfish due to observed side effects. A higher concentration of (+)-spathulenol, and lower or absent amounts of E-(-)- pinocamphone could contribute to improve the activity of the EO of A. gratissima. (+)- Spathulenol showed potent sedative and anesthetic activities in silver catfish, and could be considered as a promising anesthetic compound. |
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2015-02-112015-02-112012-02-23BENOVIT, Simone Cristina. Composition, sedative and anesthetic activity of the essential oil of Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) in silver catfish (Rhamdia quelen). 2012. 88 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/9001This work was designed to isolate the active compounds responsible for the central activity detected for the essential oil of Aloysia gratissima (EOA) in silver catfish (Rhamdia quelen). The AChE (acetylcholiesterase) activity under the influence of EOA was also investigated. Silver catfish were submitted to anesthesia bath with EOA (90, 300, 450 or 900 mg L-1), their fractions and isolated compounds to determine time of anesthetic induction and recovery. Eugenol (50 mg L-1) was used as positive control. EOA was effective as anesthetic at concentrations of 300 to 900 mg L-1. However, silver catfish presented involuntary muscle contractions during induction and recovery. AChE activity was not inhibited by EOA. The bio-guided fractionation of EOA furnished E-(-)-pinocamphone, (-)-caryophyllene oxide, (-)- guaiol and (+)-spathulenol, which were identified by mass spectrometry (MS), 1H- and 13CNMR. E-(-)-pinocamphone caused the same side effects observed to the EOA. (-)- Caryophyllene oxide, (-)-guaiol and (+)-spathulenol showed only sedative effects at concentrations proportional to those of the constituents in EOA. (-)-Caryophyllene oxide was also sedative in higher concentration (40 mg L-1) than present in EOA while (+)-spathulenol (51.2 mg L-1) promoted deep anesthesia without side effects. EOA showed anesthetic properties, however, should be used with caution in silver catfish due to observed side effects. A higher concentration of (+)-spathulenol, and lower or absent amounts of E-(-)- pinocamphone could contribute to improve the activity of the EO of A. gratissima. (+)- Spathulenol showed potent sedative and anesthetic activities in silver catfish, and could be considered as a promising anesthetic compound.Este trabalho foi desenvolvido visando isolar os compostos ativos responsáveis pela atividade anestésica detectada para o óleo essencial de Aloysia gratissima (OEA) em jundiás (Rhamdia quelen). A atividade da acetilcolinesterase (AChE) sob a influência do OEA também foi investigada. Jundiás foram submetidos a banho de anestesia com OEA (90, 300, 450 ou 900 mg L-1), suas frações e compostos isolados, a fim de determinar o tempo de indução e recuperação anestésica. Eugenol (50 mg L-1) foi utilizado como controle positivo. OEA foi eficaz como anestésico nas concentrações de 300 a 900 mg L-1. No entanto, os jundiás apresentaram contrações musculares involuntárias durante a indução e recuperação. Atividade da AChE não foi alterada pelo OEA. O fracionamento bio-guiado do OEA forneceu E-(-)-pinocafona, (-)-óxido de cariofileno, (-)-guaiol e (+)-espatulenol, os quais foram identificados por espectrometria de massas (EM), 1H e 13C-RMN. E-(-)-pinocafona causou os mesmos efeitos adversos observados para o OEA. (-)-Óxido de cariofileno, (-)-guaiol e (+)- espatulenol apresentaram apenas efeitos sedativos em concentrações proporcionais às dos constituintes no OEA. (-)-Óxido de cariofileno também foi sedativo em maior concentração (40 mg L-1) que a presente no OEA, enquanto (+)-espatulenol (51,2 mg L-1) promoveu anestesia profunda, sem efeitos adversos. OEA apresentou propriedades anestésicas, no entanto, deve ser utilizado com cuidado em jundiás devido aos efeitos adversos observados. Uma maior concentração de (+)-espatulenol, e menor quantidade ou ausência de E-(-)- pinocafona poderiam contribuir para melhorar a atividade do OE de A. gratissima. (+)- Espatulenol apresentou potente atividade sedativa e anestésica em jundiás, e pode ser considerado um composto anestésico promissor.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em FarmacologiaUFSMBRFarmacologiaAnestesiaE-(-)-pinocanfona(-)-óxido de cariofileno(-)-guaiol(+)-espatulenolAnesthesiaE-(-)-pinocamphone(-)-caryophyllene oxide(+)-spathulenolCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAComposição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen)Composition, sedative and anesthetic activity of the essential oil of Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) in silver catfish (Rhamdia quelen)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisHeinzmann, Berta Mariahttp://lattes.cnpq.br/0786124562427815Rates, Stela Maris Kuzehttp://lattes.cnpq.br/1899321687841117Cunha, Mauro Alves dahttp://lattes.cnpq.br/9745413680917763http://lattes.cnpq.br/3162093933837485Benovit, Simone Cristina201000000000400500300300300ef52d431-0698-40fb-ab56-1dbd415cc5d7c70751a8-88d3-4df8-8eef-c8210373fe3a1a936436-2c3e-4a3c-8dba-ff3790b3ab09c37f5be5-94b5-43b1-823b-ea9991138bffinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALBENOVIT, SIMONE CRISTINA.pdfapplication/pdf1857205http://repositorio.ufsm.br/bitstream/1/9001/1/BENOVIT%2c%20SIMONE%20CRISTINA.pdf219a20331f01899fa16c514843e72dd6MD51TEXTBENOVIT, SIMONE CRISTINA.pdf.txtBENOVIT, SIMONE CRISTINA.pdf.txtExtracted texttext/plain132836http://repositorio.ufsm.br/bitstream/1/9001/2/BENOVIT%2c%20SIMONE%20CRISTINA.pdf.txt921e79f9d7f31f35e02da60f84e5d4b5MD52THUMBNAILBENOVIT, SIMONE CRISTINA.pdf.jpgBENOVIT, SIMONE CRISTINA.pdf.jpgIM Thumbnailimage/jpeg4851http://repositorio.ufsm.br/bitstream/1/9001/3/BENOVIT%2c%20SIMONE%20CRISTINA.pdf.jpg8d0d5e42f187ff8177ae862f8b9f8829MD531/90012023-05-11 14:20:26.963oai:repositorio.ufsm.br:1/9001Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132023-05-11T17:20:26Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Composição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen) |
dc.title.alternative.eng.fl_str_mv |
Composition, sedative and anesthetic activity of the essential oil of Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) in silver catfish (Rhamdia quelen) |
title |
Composição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen) |
spellingShingle |
Composição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen) Benovit, Simone Cristina Anestesia E-(-)-pinocanfona (-)-óxido de cariofileno (-)-guaiol (+)-espatulenol Anesthesia E-(-)-pinocamphone (-)-caryophyllene oxide (+)-spathulenol CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Composição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen) |
title_full |
Composição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen) |
title_fullStr |
Composição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen) |
title_full_unstemmed |
Composição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen) |
title_sort |
Composição e atividade sedativa e anestésica do óleo essencial de Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) em jundiás (Rhamdia quelen) |
author |
Benovit, Simone Cristina |
author_facet |
Benovit, Simone Cristina |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Heinzmann, Berta Maria |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0786124562427815 |
dc.contributor.referee1.fl_str_mv |
Rates, Stela Maris Kuze |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1899321687841117 |
dc.contributor.referee2.fl_str_mv |
Cunha, Mauro Alves da |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9745413680917763 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3162093933837485 |
dc.contributor.author.fl_str_mv |
Benovit, Simone Cristina |
contributor_str_mv |
Heinzmann, Berta Maria Rates, Stela Maris Kuze Cunha, Mauro Alves da |
dc.subject.por.fl_str_mv |
Anestesia E-(-)-pinocanfona (-)-óxido de cariofileno (-)-guaiol (+)-espatulenol |
topic |
Anestesia E-(-)-pinocanfona (-)-óxido de cariofileno (-)-guaiol (+)-espatulenol Anesthesia E-(-)-pinocamphone (-)-caryophyllene oxide (+)-spathulenol CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
dc.subject.eng.fl_str_mv |
Anesthesia E-(-)-pinocamphone (-)-caryophyllene oxide (+)-spathulenol |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
This work was designed to isolate the active compounds responsible for the central activity detected for the essential oil of Aloysia gratissima (EOA) in silver catfish (Rhamdia quelen). The AChE (acetylcholiesterase) activity under the influence of EOA was also investigated. Silver catfish were submitted to anesthesia bath with EOA (90, 300, 450 or 900 mg L-1), their fractions and isolated compounds to determine time of anesthetic induction and recovery. Eugenol (50 mg L-1) was used as positive control. EOA was effective as anesthetic at concentrations of 300 to 900 mg L-1. However, silver catfish presented involuntary muscle contractions during induction and recovery. AChE activity was not inhibited by EOA. The bio-guided fractionation of EOA furnished E-(-)-pinocamphone, (-)-caryophyllene oxide, (-)- guaiol and (+)-spathulenol, which were identified by mass spectrometry (MS), 1H- and 13CNMR. E-(-)-pinocamphone caused the same side effects observed to the EOA. (-)- Caryophyllene oxide, (-)-guaiol and (+)-spathulenol showed only sedative effects at concentrations proportional to those of the constituents in EOA. (-)-Caryophyllene oxide was also sedative in higher concentration (40 mg L-1) than present in EOA while (+)-spathulenol (51.2 mg L-1) promoted deep anesthesia without side effects. EOA showed anesthetic properties, however, should be used with caution in silver catfish due to observed side effects. A higher concentration of (+)-spathulenol, and lower or absent amounts of E-(-)- pinocamphone could contribute to improve the activity of the EO of A. gratissima. (+)- Spathulenol showed potent sedative and anesthetic activities in silver catfish, and could be considered as a promising anesthetic compound. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-23 |
dc.date.accessioned.fl_str_mv |
2015-02-11 |
dc.date.available.fl_str_mv |
2015-02-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BENOVIT, Simone Cristina. Composition, sedative and anesthetic activity of the essential oil of Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) in silver catfish (Rhamdia quelen). 2012. 88 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/9001 |
identifier_str_mv |
BENOVIT, Simone Cristina. Composition, sedative and anesthetic activity of the essential oil of Aloysia gratissima (Gillies & Hook.) Troncoso (Verbenaceae) in silver catfish (Rhamdia quelen). 2012. 88 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
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http://repositorio.ufsm.br/handle/1/9001 |
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UFSM |
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Farmacologia |
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Universidade Federal de Santa Maria |
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