Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/11202 |
Resumo: | Mercury is a divalent metal found in the liquid state at room temperature without biological function. Occupational exposure to this metal occurs mainly in industrial activities and agriculture. Its toxicity seems to be due to affinity for sulfhydryl groups and oxidative stress induction. Studies show that mercury causes physiological and biochemical changes in animals and humans. Zinc also is a divalent metal, but in contrast, is an essential element for living beings with important metabolic functions. Studies have indicated beneficial effects of this metal against oxidative damage caused by many toxic substances, including mercury. The objective of this study was to investigate the effect of ZnCl2 and HgCl2 on effect markers and oxidative damage of lactating rats (LR) and non-lactating rats (NLR). Adults LR and NLR received one dose of 27 mg/kg of ZnCl2 (subcutaneously) and after 24 hours received one dose of 5 mg/kg HgCl2 (subcutaneously). The animals were killed 24 hours after the last administration. Kidneys, liver, brain and blood were collected to perform the biochemical test. δ-aminolevulinate dehydratase (δ-ALA-D) activity were analyzed in kidney, liver, brain, and blood; non-protein, total thiols ascorbic acid levels in kidney, liver and brain; catalase activity in kidney and liver; serum urea and creatinine levels; and alanine aminotranferase (ALT) activity in serum and liver. The weights of animals and organs were also analyzed. No alterations were observed in the brains of LR and NLR; ascorbic acid levels also was not changed in tissues analyze. LR and NLR exposed to mercury showed a decrease of kidney total SH levels and an increase of urea and creatinine levels. NLR exposed to mercury showed an inhibition of kidney, liver and blood δ-ALA-D activity, and liver catalase activity. LR exposed to mercury showed an inhibition of blood δ-ALA-D activity and serum ALT activity. LR showed a decrease of liver absolute weight and an increase of kidney relative weight. NLR showed no changes in body and organs weights. Zinc per se increased liver non-protein thiols levels in LR and NLR and decreased liver absolute weight in LR. The pre-treatment with zinc prevented the inhibition of kidney (partially), liver and blood δ-ALA-D and liver catalase activity in NLR. Zinc also prevented the inhibition of blood δ-ALA-D, serum ALT (partially), the decrease in non-protein SH levels (partially) and increase relative weight of kidneys in LR. Thus, we suggest that LR and NLR differ as the toxicity caused by mercury, and the NLR are more sensitive to these toxic effects than the LR. Zinc shows promising effects against the toxic effects on analyzed parameters. |
id |
UFSM_975e7136f6ef924daa824dd1f2d499ce |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/11202 |
network_acronym_str |
UFSM |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
|
spelling |
Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2Lactating and no-lactating rats differ in sensitivity to HgCl2: protective effect of ZnCl2MercúrioZincoLactantesEstresse oxidativoδ - aminolevulinato desidrataseMercuryZincLactatingOxidative stressδ - aminolevulinate dehydrataseCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAMercury is a divalent metal found in the liquid state at room temperature without biological function. Occupational exposure to this metal occurs mainly in industrial activities and agriculture. Its toxicity seems to be due to affinity for sulfhydryl groups and oxidative stress induction. Studies show that mercury causes physiological and biochemical changes in animals and humans. Zinc also is a divalent metal, but in contrast, is an essential element for living beings with important metabolic functions. Studies have indicated beneficial effects of this metal against oxidative damage caused by many toxic substances, including mercury. The objective of this study was to investigate the effect of ZnCl2 and HgCl2 on effect markers and oxidative damage of lactating rats (LR) and non-lactating rats (NLR). Adults LR and NLR received one dose of 27 mg/kg of ZnCl2 (subcutaneously) and after 24 hours received one dose of 5 mg/kg HgCl2 (subcutaneously). The animals were killed 24 hours after the last administration. Kidneys, liver, brain and blood were collected to perform the biochemical test. δ-aminolevulinate dehydratase (δ-ALA-D) activity were analyzed in kidney, liver, brain, and blood; non-protein, total thiols ascorbic acid levels in kidney, liver and brain; catalase activity in kidney and liver; serum urea and creatinine levels; and alanine aminotranferase (ALT) activity in serum and liver. The weights of animals and organs were also analyzed. No alterations were observed in the brains of LR and NLR; ascorbic acid levels also was not changed in tissues analyze. LR and NLR exposed to mercury showed a decrease of kidney total SH levels and an increase of urea and creatinine levels. NLR exposed to mercury showed an inhibition of kidney, liver and blood δ-ALA-D activity, and liver catalase activity. LR exposed to mercury showed an inhibition of blood δ-ALA-D activity and serum ALT activity. LR showed a decrease of liver absolute weight and an increase of kidney relative weight. NLR showed no changes in body and organs weights. Zinc per se increased liver non-protein thiols levels in LR and NLR and decreased liver absolute weight in LR. The pre-treatment with zinc prevented the inhibition of kidney (partially), liver and blood δ-ALA-D and liver catalase activity in NLR. Zinc also prevented the inhibition of blood δ-ALA-D, serum ALT (partially), the decrease in non-protein SH levels (partially) and increase relative weight of kidneys in LR. Thus, we suggest that LR and NLR differ as the toxicity caused by mercury, and the NLR are more sensitive to these toxic effects than the LR. Zinc shows promising effects against the toxic effects on analyzed parameters.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorO mercúrio é um metal divalente, encontrado no estado líquido à temperatura ambiente e sem funções biológicas. A exposição ocupacional a esse metal ocorre principalmente em atividades industriais e na agricultura. Sugere-se que sua toxicidade é devida, principalmente, à afinidade por grupamentos sulfidrílicos e indução de estresse oxidativo. Estudos demonstram que o mercúrio causa alterações fisiológicas e bioquímicas em animais e humanos. O zinco também é um metal divalente, mas, em contra partida, é um elemento essencial para os seres vivos com importantes funções metabólicas. Estudos tem apontado efeitos benéficos desse metal contra danos oxidativos causados por muitas substâncias tóxicas, inclusive o mercúrio. Assim, o objetivo desse trabalho foi investigar o efeito do ZnCl2 e do HgCl2 sobre marcadores de efeitos e de dano oxidativo em ratas lactantes (RL) e não lactantes (RNL). RL e RNL adultas receberam subcutaneamente uma dose de 27 mg/kg de ZnCl2 e após 24 horas receberam uma dose de 5 mg/kg de HgCl2. Os animais foram mortos 24 horas após a última administração. Rins, fígado, cérebro e sangue foram coletados para realização dos testes bioquímicos. Foram analisados: a atividade da δ-aminolevulinato desidratase (δ-ALA-D) renal, hepática, cerebral e sanguínea; os níveis de tióis totais e não protéicos e ácido ascórbico de rins, fígado e cérebro; atividade da catalase renal e hepática; os níveis séricos de ureia e creatinina; e a atividade da alanina aminotranferase (ALT) de soro e fígado. Os pesos dos animais e órgãos também foram analisados. Não se observou qualquer alteração nos parâmetros de cérebro das RL e RNL, e nos níveis de ácido ascórbico nos tecidos analisados. RL e RNL expostas ao mercúrio apresentaram uma diminuição nos níveis de SH total de rins e um aumento nos níveis de ureia e creatinina. RNL expostas ao mercúrio apresentaram uma inibição da atividade da δ-ALA-D de rins, fígado e sangue e da catalase hepática. RL expostas ao mercúrio tiveram uma inibição da atividade da δ-ALA-D sanguínea e da ALT sérica. Ainda, as RL apresentaram uma diminuição no peso absoluto de fígado e um aumento no peso relativo de rins. RNL não apresentaram alterações do peso corporal e dos órgãos. O zinco per se aumentou os níveis de tióis não protéicos de fígado nas RL e RNL e diminuiu o peso absoluto de fígado nas RL. O pré-tratamento com zinco preveniu a inibição da δ-ALA-D renal (parcialmente), hepática e sanguínea e da catalase hepática nas RNL. O zinco também preveniu a inibição da δ-ALA-D sanguínea, da ALT sérica (parcialmente), a diminuição nos níveis de SH não protéicos (parcialmente) e aumento do peso relativo de rins nas RL. Dessa forma, podemos sugerir que RL e RNL diferem quanto à toxicidade causada pelo mercúrio, visto que as RNL são mais sensíveis a esses efeitos tóxicos do que as RL, e que o zinco apresenta efeitos promissores contra essa ação tóxica na maioria dos parâmetros analisados.Universidade Federal de Santa MariaBRBioquímicaUFSMPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaPereira, Maria Esterhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728086Y2Brandão, Ricardohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4779496T3Morsch, Vera Mariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784273E6Oliveira, Vitor Antunes de2013-06-212013-06-212012-08-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfOLIVEIRA, Vitor Antunes de. Lactating and no-lactating rats differ in sensitivity to HgCl2: protective effect of ZnCl2. 2012. 66 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/11202porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2017-07-26T23:59:44Zoai:repositorio.ufsm.br:1/11202Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-07-26T23:59:44Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2 Lactating and no-lactating rats differ in sensitivity to HgCl2: protective effect of ZnCl2 |
title |
Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2 |
spellingShingle |
Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2 Oliveira, Vitor Antunes de Mercúrio Zinco Lactantes Estresse oxidativo δ - aminolevulinato desidratase Mercury Zinc Lactating Oxidative stress δ - aminolevulinate dehydratase CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2 |
title_full |
Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2 |
title_fullStr |
Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2 |
title_full_unstemmed |
Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2 |
title_sort |
Ratas lactantes e não lactantes diferem quanto à sensibilidade ao HgCl2: efeito protetor do ZnCl2 |
author |
Oliveira, Vitor Antunes de |
author_facet |
Oliveira, Vitor Antunes de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Pereira, Maria Ester http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4728086Y2 Brandão, Ricardo http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4779496T3 Morsch, Vera Maria http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784273E6 |
dc.contributor.author.fl_str_mv |
Oliveira, Vitor Antunes de |
dc.subject.por.fl_str_mv |
Mercúrio Zinco Lactantes Estresse oxidativo δ - aminolevulinato desidratase Mercury Zinc Lactating Oxidative stress δ - aminolevulinate dehydratase CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
topic |
Mercúrio Zinco Lactantes Estresse oxidativo δ - aminolevulinato desidratase Mercury Zinc Lactating Oxidative stress δ - aminolevulinate dehydratase CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Mercury is a divalent metal found in the liquid state at room temperature without biological function. Occupational exposure to this metal occurs mainly in industrial activities and agriculture. Its toxicity seems to be due to affinity for sulfhydryl groups and oxidative stress induction. Studies show that mercury causes physiological and biochemical changes in animals and humans. Zinc also is a divalent metal, but in contrast, is an essential element for living beings with important metabolic functions. Studies have indicated beneficial effects of this metal against oxidative damage caused by many toxic substances, including mercury. The objective of this study was to investigate the effect of ZnCl2 and HgCl2 on effect markers and oxidative damage of lactating rats (LR) and non-lactating rats (NLR). Adults LR and NLR received one dose of 27 mg/kg of ZnCl2 (subcutaneously) and after 24 hours received one dose of 5 mg/kg HgCl2 (subcutaneously). The animals were killed 24 hours after the last administration. Kidneys, liver, brain and blood were collected to perform the biochemical test. δ-aminolevulinate dehydratase (δ-ALA-D) activity were analyzed in kidney, liver, brain, and blood; non-protein, total thiols ascorbic acid levels in kidney, liver and brain; catalase activity in kidney and liver; serum urea and creatinine levels; and alanine aminotranferase (ALT) activity in serum and liver. The weights of animals and organs were also analyzed. No alterations were observed in the brains of LR and NLR; ascorbic acid levels also was not changed in tissues analyze. LR and NLR exposed to mercury showed a decrease of kidney total SH levels and an increase of urea and creatinine levels. NLR exposed to mercury showed an inhibition of kidney, liver and blood δ-ALA-D activity, and liver catalase activity. LR exposed to mercury showed an inhibition of blood δ-ALA-D activity and serum ALT activity. LR showed a decrease of liver absolute weight and an increase of kidney relative weight. NLR showed no changes in body and organs weights. Zinc per se increased liver non-protein thiols levels in LR and NLR and decreased liver absolute weight in LR. The pre-treatment with zinc prevented the inhibition of kidney (partially), liver and blood δ-ALA-D and liver catalase activity in NLR. Zinc also prevented the inhibition of blood δ-ALA-D, serum ALT (partially), the decrease in non-protein SH levels (partially) and increase relative weight of kidneys in LR. Thus, we suggest that LR and NLR differ as the toxicity caused by mercury, and the NLR are more sensitive to these toxic effects than the LR. Zinc shows promising effects against the toxic effects on analyzed parameters. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-17 2013-06-21 2013-06-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
OLIVEIRA, Vitor Antunes de. Lactating and no-lactating rats differ in sensitivity to HgCl2: protective effect of ZnCl2. 2012. 66 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. http://repositorio.ufsm.br/handle/1/11202 |
identifier_str_mv |
OLIVEIRA, Vitor Antunes de. Lactating and no-lactating rats differ in sensitivity to HgCl2: protective effect of ZnCl2. 2012. 66 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
url |
http://repositorio.ufsm.br/handle/1/11202 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Bioquímica UFSM Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922106832060416 |