Obtenção de bioherbicida a partir de fungo isolado do bioma Mata Atlântica
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/18034 |
Resumo: | The search for decreases in losses of agricultural production makes it necessary to use pesticides to combat pests and weeds. The use of products derived from the chemical industry on disease control in agriculture has been questioned because of its adverses effects. As a result, many synthetic agricultural products were removed from markets, which creates the need for development of alternative means of weed management. There is an interest in the discovery and development of new bioherbicides from micro-organisms, especially fungi, and it’s an important tool in biological control. Therefore, the aim of this work was to produce a bioherbicide from submerged fermentation of the fungus Fusarium graminearum isolated from the Atlantic Forest biome and evaluate its phytotoxic potential. Initially a experimental design Plackett Burman type was applied, and the variables measured in biomass yield were pH (5-7), glucose (5-15 g.L-1), peptone (5-15 g.L-1) and yeast extract (5-10 g.L-1). Only peptone had a significant positive effect on biomass yield, so new tests with different concentrations of peptone (10-80 g.L-1) were conducted. and post-emergence test was applied with Cucumis sativus . var. wisconsin plants (cucumber) after 21 days. At the first post-emergence evaluation for the phytotoxicity parameter the best results occurred at a concentration of 40 g.L-1 peptone. In this condition, two treatments, partial lyophilization and membrane separation were used for concentration of the broth. In the evaluation of phytopathogen action after application of the treated supernatant was observed reduction of 100% of the length of the roots, reduced shoot growth and visually greater phytotoxicity in treatment using the permeated fraction of the membrane separation process. Among the compounds identified through gas chromatography, there was the presence of nitrogenous heterocyclic, including: pyrimidines, piperazines, pyrrolidines, piperidines, aziridines, quinolines and tetrazoles, but other studies are needed to define which one (s) toxin (s) are causing the phytotoxic effect. |
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2019-08-27T12:38:43Z2019-08-27T12:38:43Z2016-08-22http://repositorio.ufsm.br/handle/1/18034The search for decreases in losses of agricultural production makes it necessary to use pesticides to combat pests and weeds. The use of products derived from the chemical industry on disease control in agriculture has been questioned because of its adverses effects. As a result, many synthetic agricultural products were removed from markets, which creates the need for development of alternative means of weed management. There is an interest in the discovery and development of new bioherbicides from micro-organisms, especially fungi, and it’s an important tool in biological control. Therefore, the aim of this work was to produce a bioherbicide from submerged fermentation of the fungus Fusarium graminearum isolated from the Atlantic Forest biome and evaluate its phytotoxic potential. Initially a experimental design Plackett Burman type was applied, and the variables measured in biomass yield were pH (5-7), glucose (5-15 g.L-1), peptone (5-15 g.L-1) and yeast extract (5-10 g.L-1). Only peptone had a significant positive effect on biomass yield, so new tests with different concentrations of peptone (10-80 g.L-1) were conducted. and post-emergence test was applied with Cucumis sativus . var. wisconsin plants (cucumber) after 21 days. At the first post-emergence evaluation for the phytotoxicity parameter the best results occurred at a concentration of 40 g.L-1 peptone. In this condition, two treatments, partial lyophilization and membrane separation were used for concentration of the broth. In the evaluation of phytopathogen action after application of the treated supernatant was observed reduction of 100% of the length of the roots, reduced shoot growth and visually greater phytotoxicity in treatment using the permeated fraction of the membrane separation process. Among the compounds identified through gas chromatography, there was the presence of nitrogenous heterocyclic, including: pyrimidines, piperazines, pyrrolidines, piperidines, aziridines, quinolines and tetrazoles, but other studies are needed to define which one (s) toxin (s) are causing the phytotoxic effect.A busca pela diminuição de perdas na produção agrícola torna necessária a utilização de agroquímicos no combate de pragas e plantas daninhas. O uso de produtos derivados da indústria química no controle de doenças na agricultura tem sido questionado em função de seus efeitos adversos. Em virtude disso, muitos produtos agrícolas sintéticos foram retirados do mercado, o que cria a necessidade de desenvolver meios alternativos para manejo das plantas daninhas. Para isso, existe um interesse na descoberta e desenvolvimento de novos bioherbicidas, a partir de micro-organismos, principalmente fungos, sendo uma ferramenta importante no controle biológico. Portanto, o objetivo deste trabalho foi produzir um bioherbicida por fermentação submersa a partir do fungo Fusarium graminearum isolado do Bioma Mata Atlântica e avaliar o seu potencial fitotóxico. Inicialmente um planejamento do tipo Plackett Burman foi aplicado, onde as variáveis avaliadas na produção de biomassa foram pH (5-7), glicose (5-15 g.L-1), peptona (5-15 g.L-1) e extrato de levedura (5-10 g.L-1). Somente a peptona apresentou efeito positivo significativo na produção de biomassa, portanto, novos ensaios com diferentes concentrações de peptona (10-80 g.L-1) foram conduzidos, sendo avaliados a produção de biomassa e aplicação em pós-emergência em plantas teste Cucumis sativus . var. wisconsin (pepino) após 21 dias. Na primeira avaliação em pós-emergência, para o parâmetro de fitotoxicidade o melhor resultado ocorreu na concentração de 40 g.L-1 de peptona. Nesta condição, dois tratamentos, liofilização parcial e separação por membranas, foram aplicados para concentração do caldo. Na avaliação da ação fitopatógena após aplicação do sobrenadante tratado observou-se redução de 100% do comprimento das raízes, redução no crescimento da parte aérea e visualmente maior fitotoxicidade, no tratamento utilizando a fração permeada do processo de separação por membranas. Entre os compostos identificados através de cromatografia gasosa, destaca-se a presença de heterocíclicos nitrogenados, entre eles: pirimidinas, piperazinas, pirrolidinas, piperidinas, aziridinas, quinolinas e tetrazóis, porém outros estudos são necessários para definir qual(is) a(s) toxina(s) estão causando o efeito fitotóxico.porUniversidade Federal de Santa MariaCentro de TecnologiaPrograma de Pós-Graduação em Engenharia QuímicaUFSMBrasilEngenharia QuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessBioherbicidaFusarium graminearumFermentação submersaFitotoxinasBioherbicideFusarium graminearumSubmerged fermentationPhytotoxinsCNPQ::ENGENHARIAS::ENGENHARIA QUIMICAObtenção de bioherbicida a partir de fungo isolado do bioma Mata AtlânticaObtainment of bioherbicide from isolated fungi of Atlantic Forest biomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTerra, Lisiane de Marsillachttp://lattes.cnpq.br/3695292052760336Kuhn, Raquel Cristinehttp://lattes.cnpq.br/1479407050883691Ziotti, Ana Paula Manerahttp://lattes.cnpq.br/1278580760766355Mazutti, Marcio Antoniohttp://lattes.cnpq.br/3520282081196395http://lattes.cnpq.br/0799177059776747Bastos, Juliana de Oliveira300600000006600abecbeeb-4b09-4f77-94e7-2742b9914f3219506668-0117-4b01-b24c-f5d3e9b83b4b8faee297-3cc7-4eb3-af92-cc0900b01d5a3a52e21d-4d04-4f9e-b604-80a9ed9cd6fc841a0160-8731-4bc5-98ff-9fb9e3de44bcreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGEQ_2016_BASTOS_JULIANA.pdfDIS_PPGEQ_2016_BASTOS_JULIANA.pdfDissertação de Mestradoapplication/pdf2268709http://repositorio.ufsm.br/bitstream/1/18034/1/DIS_PPGEQ_2016_BASTOS_JULIANA.pdf4efe84179104d2c815812e1f009b472eMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/18034/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-816http://repositorio.ufsm.br/bitstream/1/18034/3/license.txtf8fcb28efb1c8cf0dc096bec902bf4c4MD53TEXTDIS_PPGEQ_2016_BASTOS_JULIANA.pdf.txtDIS_PPGEQ_2016_BASTOS_JULIANA.pdf.txtExtracted texttext/plain152755http://repositorio.ufsm.br/bitstream/1/18034/4/DIS_PPGEQ_2016_BASTOS_JULIANA.pdf.txt0e64230d1b6c7cce9df510a5d1774420MD54THUMBNAILDIS_PPGEQ_2016_BASTOS_JULIANA.pdf.jpgDIS_PPGEQ_2016_BASTOS_JULIANA.pdf.jpgIM Thumbnailimage/jpeg4130http://repositorio.ufsm.br/bitstream/1/18034/5/DIS_PPGEQ_2016_BASTOS_JULIANA.pdf.jpg302069b581dc38ef56bfacf5f015de04MD551/180342019-08-28 03:01:29.332oai:repositorio.ufsm.br:1/18034Q3JlYXRpdmUgQ29tbW9ucw==Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132019-08-28T06:01:29Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Obtenção de bioherbicida a partir de fungo isolado do bioma Mata Atlântica |
dc.title.alternative.eng.fl_str_mv |
Obtainment of bioherbicide from isolated fungi of Atlantic Forest biome |
title |
Obtenção de bioherbicida a partir de fungo isolado do bioma Mata Atlântica |
spellingShingle |
Obtenção de bioherbicida a partir de fungo isolado do bioma Mata Atlântica Bastos, Juliana de Oliveira Bioherbicida Fusarium graminearum Fermentação submersa Fitotoxinas Bioherbicide Fusarium graminearum Submerged fermentation Phytotoxins CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Obtenção de bioherbicida a partir de fungo isolado do bioma Mata Atlântica |
title_full |
Obtenção de bioherbicida a partir de fungo isolado do bioma Mata Atlântica |
title_fullStr |
Obtenção de bioherbicida a partir de fungo isolado do bioma Mata Atlântica |
title_full_unstemmed |
Obtenção de bioherbicida a partir de fungo isolado do bioma Mata Atlântica |
title_sort |
Obtenção de bioherbicida a partir de fungo isolado do bioma Mata Atlântica |
author |
Bastos, Juliana de Oliveira |
author_facet |
Bastos, Juliana de Oliveira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Terra, Lisiane de Marsillac |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3695292052760336 |
dc.contributor.advisor-co1.fl_str_mv |
Kuhn, Raquel Cristine |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/1479407050883691 |
dc.contributor.referee1.fl_str_mv |
Ziotti, Ana Paula Manera |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1278580760766355 |
dc.contributor.referee2.fl_str_mv |
Mazutti, Marcio Antonio |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3520282081196395 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0799177059776747 |
dc.contributor.author.fl_str_mv |
Bastos, Juliana de Oliveira |
contributor_str_mv |
Terra, Lisiane de Marsillac Kuhn, Raquel Cristine Ziotti, Ana Paula Manera Mazutti, Marcio Antonio |
dc.subject.por.fl_str_mv |
Bioherbicida Fusarium graminearum Fermentação submersa Fitotoxinas |
topic |
Bioherbicida Fusarium graminearum Fermentação submersa Fitotoxinas Bioherbicide Fusarium graminearum Submerged fermentation Phytotoxins CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
Bioherbicide Fusarium graminearum Submerged fermentation Phytotoxins |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
description |
The search for decreases in losses of agricultural production makes it necessary to use pesticides to combat pests and weeds. The use of products derived from the chemical industry on disease control in agriculture has been questioned because of its adverses effects. As a result, many synthetic agricultural products were removed from markets, which creates the need for development of alternative means of weed management. There is an interest in the discovery and development of new bioherbicides from micro-organisms, especially fungi, and it’s an important tool in biological control. Therefore, the aim of this work was to produce a bioherbicide from submerged fermentation of the fungus Fusarium graminearum isolated from the Atlantic Forest biome and evaluate its phytotoxic potential. Initially a experimental design Plackett Burman type was applied, and the variables measured in biomass yield were pH (5-7), glucose (5-15 g.L-1), peptone (5-15 g.L-1) and yeast extract (5-10 g.L-1). Only peptone had a significant positive effect on biomass yield, so new tests with different concentrations of peptone (10-80 g.L-1) were conducted. and post-emergence test was applied with Cucumis sativus . var. wisconsin plants (cucumber) after 21 days. At the first post-emergence evaluation for the phytotoxicity parameter the best results occurred at a concentration of 40 g.L-1 peptone. In this condition, two treatments, partial lyophilization and membrane separation were used for concentration of the broth. In the evaluation of phytopathogen action after application of the treated supernatant was observed reduction of 100% of the length of the roots, reduced shoot growth and visually greater phytotoxicity in treatment using the permeated fraction of the membrane separation process. Among the compounds identified through gas chromatography, there was the presence of nitrogenous heterocyclic, including: pyrimidines, piperazines, pyrrolidines, piperidines, aziridines, quinolines and tetrazoles, but other studies are needed to define which one (s) toxin (s) are causing the phytotoxic effect. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-08-22 |
dc.date.accessioned.fl_str_mv |
2019-08-27T12:38:43Z |
dc.date.available.fl_str_mv |
2019-08-27T12:38:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/18034 |
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http://repositorio.ufsm.br/handle/1/18034 |
dc.language.iso.fl_str_mv |
por |
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por |
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300600000006 |
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600 |
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abecbeeb-4b09-4f77-94e7-2742b9914f32 19506668-0117-4b01-b24c-f5d3e9b83b4b 8faee297-3cc7-4eb3-af92-cc0900b01d5a 3a52e21d-4d04-4f9e-b604-80a9ed9cd6fc 841a0160-8731-4bc5-98ff-9fb9e3de44bc |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade Federal de Santa Maria Centro de Tecnologia |
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Programa de Pós-Graduação em Engenharia Química |
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UFSM |
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Brasil |
dc.publisher.department.fl_str_mv |
Engenharia Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Tecnologia |
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