Avaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/17261 |
Resumo: | Reserpine administration is considered an animal model for the study of tardive dyskinesia by some authors and a model for the study of parkinsonism by others. However, despite several studies used this model to assess the potential of substances to treat these movement disorders, little is known about the mechanisms involved in the development of behavioral changes in reserpine model. Thus, the present study investigated whether behavioral alterations induced by reserpine are related to alterations in dopaminergic system proteins as tyrosine hydroxylase (TH), dopamine transporter (DAT) and monoaminoxidase (MAO). For this, reserpine was administered subcutaneously in mice at doses of 0.1, 0.5 or 1 mg / kg or vehicle (0.2% acetic acid in NaCl 0.9%) for 4 consecutive days. The number of vacuous chewing movements (VCMs), exploratory and locomotor activity were assessed 48 hours (6th day) and 20 days (24th day) after withdrawal of treatment in order to evaluate the induction and maintenance of motor disorders caused by reserpine. It was also analyzed the TH and DAT immunoreactivity by Western blot and the MAO-A and MAO-B activity in striatum and region containing substantia nigra on days 6 and 24. Treatment with 1 mg/kg reserpine caused an increase in VCMs and hypolocomotion in animals, and this effect remained for at least 20 days after withdrawal of reserpine. These alterations were accompanied by a reduction in striatal DAT immunoreactivity and a reduction of TH immunoreactivity in the substantia nigra evaluated on day 6. On the 24th day was observed a decrease in the DAT and TH immunoreactivity in both striatum and substantia nigra. The dose of 0.5 mg/kg reserpine caused behavioral alterations on day 6, but these changes were not maintained after withdrawal of treatment and also neurochemical changes not were found at this dose. We did not find any statistical differences when behavioral and neurochemical parameters were evaluated at a dose of 0.1 mg/kg reserpine. Thereby, these results suggest that reserpine causes changes in dopaminergic system proteins which lead to motor alterations. Thus, possible pharmacological interventions in these proteins could ameliorate motor symptoms in both, Parkinson's disease and tardive dyskinesia. |
id |
UFSM-20_9a60a570f7ff4f2d397c1e2eb10ee23a |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/17261 |
network_acronym_str |
UFSM-20 |
network_name_str |
Repositório Institucional Manancial UFSM |
repository_id_str |
3913 |
spelling |
2019-07-02T19:03:01Z2019-07-02T19:03:01Z2014-10-02http://repositorio.ufsm.br/handle/1/17261Reserpine administration is considered an animal model for the study of tardive dyskinesia by some authors and a model for the study of parkinsonism by others. However, despite several studies used this model to assess the potential of substances to treat these movement disorders, little is known about the mechanisms involved in the development of behavioral changes in reserpine model. Thus, the present study investigated whether behavioral alterations induced by reserpine are related to alterations in dopaminergic system proteins as tyrosine hydroxylase (TH), dopamine transporter (DAT) and monoaminoxidase (MAO). For this, reserpine was administered subcutaneously in mice at doses of 0.1, 0.5 or 1 mg / kg or vehicle (0.2% acetic acid in NaCl 0.9%) for 4 consecutive days. The number of vacuous chewing movements (VCMs), exploratory and locomotor activity were assessed 48 hours (6th day) and 20 days (24th day) after withdrawal of treatment in order to evaluate the induction and maintenance of motor disorders caused by reserpine. It was also analyzed the TH and DAT immunoreactivity by Western blot and the MAO-A and MAO-B activity in striatum and region containing substantia nigra on days 6 and 24. Treatment with 1 mg/kg reserpine caused an increase in VCMs and hypolocomotion in animals, and this effect remained for at least 20 days after withdrawal of reserpine. These alterations were accompanied by a reduction in striatal DAT immunoreactivity and a reduction of TH immunoreactivity in the substantia nigra evaluated on day 6. On the 24th day was observed a decrease in the DAT and TH immunoreactivity in both striatum and substantia nigra. The dose of 0.5 mg/kg reserpine caused behavioral alterations on day 6, but these changes were not maintained after withdrawal of treatment and also neurochemical changes not were found at this dose. We did not find any statistical differences when behavioral and neurochemical parameters were evaluated at a dose of 0.1 mg/kg reserpine. Thereby, these results suggest that reserpine causes changes in dopaminergic system proteins which lead to motor alterations. Thus, possible pharmacological interventions in these proteins could ameliorate motor symptoms in both, Parkinson's disease and tardive dyskinesia.A administração de reserpina é considerada um modelo animal para o estudo da discinesia tardia por alguns autores e um modelo para estudo do parkinsonismo por outros. No entanto, apesar de inúmeros trabalhos utilizarem este modelo para avaliar o potencial de substâncias para o tratamento desses distúrbios do movimento, pouco se sabe sobre os mecanismos envolvidos no desenvolvimento das alterações comportamentais presentes no modelo da reserpina. Assim, o presente estudo investigou se as alterações motoras induzidas pela reserpina estão relacionados com alterações em proteínas do sistema dopaminérgico como a tirosina hidroxilase (TH), o transportador de dopamina (TDA) e monoaminoxidase (MAO). Para isso, a reserpina foi administrada subcutaneamente em camundongos nas doses de 0,1, 0,5 ou 1 mg/kg ou veículo (0,2% ácido acético em NaCl 0,9%) durante 4 dias consecutivos. O número de movimentos de mascar no vazio (MMVs), atividade locomotora e exploratória foram avaliados 48 horas (6º dia) e 20 dias (24º dia) após a retirada do tratamento, a fim de avaliar a indução bem como a manutenção das alterações motoras causadas pela reserpina. Foram também analisados a imunoreatividade da TH e do TDA por Western Blot e a atividade da MAO-A e MAO-B em estriado e região contendo a substantia nigra no 6º e 24º dia. O tratamento com 1 mg/kg de reserpina causou um aumento dos MMVs e hipolocomoção nos animais e este efeito se manteve por pelo menos 20 dias após a retirada da reserpina. Essas alterações foram acompanhadas por uma redução na imunoreatividade do TDA no estriado e redução da TH na substantia nigra avaliadas no 6º dia. No 24º dia foi observada uma diminuição na imunoreatividade da TH e do TDA tanto no estriado quanto na substantia nigra. A dose de 0,5 mg/kg de reserpina causou alterações comportamentais no 6º dia, mas estas alterações não se mantiveram após a retirada do tratamento e também não foram encontradas alterações neuroquímicas nesta dose. Não foram encontradas diferenças estatisticamente significativas quando os parâmetros comportamentais e neuroquímicos foram avaliados na dose de 0,1 mg/kg de reserpina. Assim, esses resultados sugerem que a reserpina causa alterações em proteínas do sistema dopaminérgico que conduzem às alterações motoras. Desta forma, possíveis intervenções farmacológicas nessas proteínas poderiam aliviar os sintomas motores tanto na doença de Parkinson quanto na discinesia tardia.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessReserpinaTirosina hidroxilaseTransportador de dopaminaDiscinesia tardiaDoença de ParkinsonReserpineTyrosine hydroxylaseDopamine transporterTardive dyskinesiaParkinson's diseaseCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAvaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongosEvaluation of behavioral and neurochemical effects induced by different doses of reserpine in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisFachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306Oliveira, Mauro Schneiderhttp://lattes.cnpq.br/7132934163734175Barbosa, Nilda Berenice de Vargashttp://lattes.cnpq.br/5901511067144019http://lattes.cnpq.br/1871026716156302Freitas, Catiuscia Molz de200800000002600e99bf03a-61e7-46c9-97d1-a7255476d47f53767f1e-9375-4538-a4d5-1ca76e96ac8a10d2124b-3909-4951-a54e-a167355aa755992f73ff-2527-4381-9778-8806800c0f42reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGBT_2014_FREITAS_CATIUSCIA.pdfDIS_PPGBT_2014_FREITAS_CATIUSCIA.pdfDissertação de Mestradoapplication/pdf1109508http://repositorio.ufsm.br/bitstream/1/17261/1/DIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf9b2706fb44b6fd409a20d318baf1d20eMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/17261/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/17261/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53TEXTDIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf.txtDIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf.txtExtracted texttext/plain102872http://repositorio.ufsm.br/bitstream/1/17261/4/DIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf.txt4568685f74689e853f1656d388ecd0a6MD54THUMBNAILDIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf.jpgDIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf.jpgIM Thumbnailimage/jpeg4981http://repositorio.ufsm.br/bitstream/1/17261/5/DIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf.jpga4f507593069f5d1f3ef57e9ae75d4c3MD551/172612022-08-17 11:12:10.006oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132022-08-17T14:12:10Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Avaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongos |
dc.title.alternative.eng.fl_str_mv |
Evaluation of behavioral and neurochemical effects induced by different doses of reserpine in mice |
title |
Avaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongos |
spellingShingle |
Avaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongos Freitas, Catiuscia Molz de Reserpina Tirosina hidroxilase Transportador de dopamina Discinesia tardia Doença de Parkinson Reserpine Tyrosine hydroxylase Dopamine transporter Tardive dyskinesia Parkinson's disease CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Avaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongos |
title_full |
Avaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongos |
title_fullStr |
Avaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongos |
title_full_unstemmed |
Avaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongos |
title_sort |
Avaliação dos efeitos comportamentais e neuroquímicos induzidos por diferentes doses de reserpina em camundongos |
author |
Freitas, Catiuscia Molz de |
author_facet |
Freitas, Catiuscia Molz de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Fachinetto, Roselei |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7203076675431306 |
dc.contributor.referee1.fl_str_mv |
Oliveira, Mauro Schneider |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7132934163734175 |
dc.contributor.referee2.fl_str_mv |
Barbosa, Nilda Berenice de Vargas |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5901511067144019 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1871026716156302 |
dc.contributor.author.fl_str_mv |
Freitas, Catiuscia Molz de |
contributor_str_mv |
Fachinetto, Roselei Oliveira, Mauro Schneider Barbosa, Nilda Berenice de Vargas |
dc.subject.por.fl_str_mv |
Reserpina Tirosina hidroxilase Transportador de dopamina Discinesia tardia Doença de Parkinson |
topic |
Reserpina Tirosina hidroxilase Transportador de dopamina Discinesia tardia Doença de Parkinson Reserpine Tyrosine hydroxylase Dopamine transporter Tardive dyskinesia Parkinson's disease CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Reserpine Tyrosine hydroxylase Dopamine transporter Tardive dyskinesia Parkinson's disease |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Reserpine administration is considered an animal model for the study of tardive dyskinesia by some authors and a model for the study of parkinsonism by others. However, despite several studies used this model to assess the potential of substances to treat these movement disorders, little is known about the mechanisms involved in the development of behavioral changes in reserpine model. Thus, the present study investigated whether behavioral alterations induced by reserpine are related to alterations in dopaminergic system proteins as tyrosine hydroxylase (TH), dopamine transporter (DAT) and monoaminoxidase (MAO). For this, reserpine was administered subcutaneously in mice at doses of 0.1, 0.5 or 1 mg / kg or vehicle (0.2% acetic acid in NaCl 0.9%) for 4 consecutive days. The number of vacuous chewing movements (VCMs), exploratory and locomotor activity were assessed 48 hours (6th day) and 20 days (24th day) after withdrawal of treatment in order to evaluate the induction and maintenance of motor disorders caused by reserpine. It was also analyzed the TH and DAT immunoreactivity by Western blot and the MAO-A and MAO-B activity in striatum and region containing substantia nigra on days 6 and 24. Treatment with 1 mg/kg reserpine caused an increase in VCMs and hypolocomotion in animals, and this effect remained for at least 20 days after withdrawal of reserpine. These alterations were accompanied by a reduction in striatal DAT immunoreactivity and a reduction of TH immunoreactivity in the substantia nigra evaluated on day 6. On the 24th day was observed a decrease in the DAT and TH immunoreactivity in both striatum and substantia nigra. The dose of 0.5 mg/kg reserpine caused behavioral alterations on day 6, but these changes were not maintained after withdrawal of treatment and also neurochemical changes not were found at this dose. We did not find any statistical differences when behavioral and neurochemical parameters were evaluated at a dose of 0.1 mg/kg reserpine. Thereby, these results suggest that reserpine causes changes in dopaminergic system proteins which lead to motor alterations. Thus, possible pharmacological interventions in these proteins could ameliorate motor symptoms in both, Parkinson's disease and tardive dyskinesia. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-10-02 |
dc.date.accessioned.fl_str_mv |
2019-07-02T19:03:01Z |
dc.date.available.fl_str_mv |
2019-07-02T19:03:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/17261 |
url |
http://repositorio.ufsm.br/handle/1/17261 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200800000002 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
e99bf03a-61e7-46c9-97d1-a7255476d47f 53767f1e-9375-4538-a4d5-1ca76e96ac8a 10d2124b-3909-4951-a54e-a167355aa755 992f73ff-2527-4381-9778-8806800c0f42 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional Manancial UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Repositório Institucional Manancial UFSM |
collection |
Repositório Institucional Manancial UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/17261/1/DIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf http://repositorio.ufsm.br/bitstream/1/17261/2/license_rdf http://repositorio.ufsm.br/bitstream/1/17261/3/license.txt http://repositorio.ufsm.br/bitstream/1/17261/4/DIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf.txt http://repositorio.ufsm.br/bitstream/1/17261/5/DIS_PPGBT_2014_FREITAS_CATIUSCIA.pdf.jpg |
bitstream.checksum.fl_str_mv |
9b2706fb44b6fd409a20d318baf1d20e 4460e5956bc1d1639be9ae6146a50347 2f0571ecee68693bd5cd3f17c1e075df 4568685f74689e853f1656d388ecd0a6 a4f507593069f5d1f3ef57e9ae75d4c3 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
ouvidoria@ufsm.br |
_version_ |
1808854725443977216 |