Óleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicas
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/8744 |
Resumo: | Researches involving plant derived products aiming to discover extractives useful for different purposes have been growing, and among them, essential oils (EO) are noteworthy. One of the main sources of EO is the Lamiaceae family. Belonging to this family, Hesperozygis ringens (Benth.) Epling is a native species from the Pampa biome, popularly known as espanta-pulga . This study aimed to evaluate the biological potential of EO from its leaves as allelopathic and larvicidal, as well as to check the seasonal and individual variability of yield and chemical composition of this oil. EO of four specimens were obtained from leaves collected in different seasons of a year and had their yield (% m/m) and chemical compositions determined. EO were extracted by hidrodestillation in Clevenger apparatus and analyzed by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID). The allelopathic potential of the EO was tested against seeds of Dicots and Monocots classified as bioindicators [Avena strigosa Schreb. and Lactuca sativa L.], weeds [Lolium multiflorum Lam and Bidens pilosa L.] and crops [Oryza sativa L. and Glycine max (L.) Merr.] by observing the following variables: germination, emergence velocity index, root length, shoot lenght and total length of seedlings. The tested concentrations were 0.25, 0.50, 1.00, 2.50 and 5.00%.The EO of H. ringens had also its larvicidal activity tested against larvae of Odonata in order to determine its 50% lethal concentration (LC50). Regarding the allelopathic tests on Dicotyledons, EO with 82.02% of pulegone inhibited completely the germination of B. pilosa at 5.00%, without affecting significantly L. sativa, but slowing the emergence and damaging root lengths and seedlings of G. max. Regarding the monocots, the same concentration strongly impaired L. multiflorum, without affecting the germination of O. sativa, but it influenced negatively the other evaluated parameters. In addition, the development of the seeds of A. strigosa was completely inhibited at the concentration of 2.50%. The yield of EO proved to be influenced by seasonality, being higher in spring, summer, and autumn. The yield also showed individual variability (1.15 a 4.38%), proving to be higher in specimen 4. The chemical composition of EO obtained from leaves collected in autumn, spring, and summer showed similar results. The production of the major constituent (pulegone) and the predominant class (oxygenated monoterpenoids) were favored in spring, summer, and autumn. These seasons proved to be more indicated for the EO extraction, since they enabled concomitantly higher yields and higher contents of oxygenated monoterpenoids and pulegone. Regarding the larvicidal activity, EO containing 95.18% of pulegone showed a LC50 of 69.05 µL L-1 against the Odonata larvae. |
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2014-12-012014-12-012014-02-25PINHEIRO, Carlos Garrido. Essential oil of Hesperozygis ringens (Benth.) Epling: variability of yield, chemical composition and biological activities. 2014. 91 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/8744Researches involving plant derived products aiming to discover extractives useful for different purposes have been growing, and among them, essential oils (EO) are noteworthy. One of the main sources of EO is the Lamiaceae family. Belonging to this family, Hesperozygis ringens (Benth.) Epling is a native species from the Pampa biome, popularly known as espanta-pulga . This study aimed to evaluate the biological potential of EO from its leaves as allelopathic and larvicidal, as well as to check the seasonal and individual variability of yield and chemical composition of this oil. EO of four specimens were obtained from leaves collected in different seasons of a year and had their yield (% m/m) and chemical compositions determined. EO were extracted by hidrodestillation in Clevenger apparatus and analyzed by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID). The allelopathic potential of the EO was tested against seeds of Dicots and Monocots classified as bioindicators [Avena strigosa Schreb. and Lactuca sativa L.], weeds [Lolium multiflorum Lam and Bidens pilosa L.] and crops [Oryza sativa L. and Glycine max (L.) Merr.] by observing the following variables: germination, emergence velocity index, root length, shoot lenght and total length of seedlings. The tested concentrations were 0.25, 0.50, 1.00, 2.50 and 5.00%.The EO of H. ringens had also its larvicidal activity tested against larvae of Odonata in order to determine its 50% lethal concentration (LC50). Regarding the allelopathic tests on Dicotyledons, EO with 82.02% of pulegone inhibited completely the germination of B. pilosa at 5.00%, without affecting significantly L. sativa, but slowing the emergence and damaging root lengths and seedlings of G. max. Regarding the monocots, the same concentration strongly impaired L. multiflorum, without affecting the germination of O. sativa, but it influenced negatively the other evaluated parameters. In addition, the development of the seeds of A. strigosa was completely inhibited at the concentration of 2.50%. The yield of EO proved to be influenced by seasonality, being higher in spring, summer, and autumn. The yield also showed individual variability (1.15 a 4.38%), proving to be higher in specimen 4. The chemical composition of EO obtained from leaves collected in autumn, spring, and summer showed similar results. The production of the major constituent (pulegone) and the predominant class (oxygenated monoterpenoids) were favored in spring, summer, and autumn. These seasons proved to be more indicated for the EO extraction, since they enabled concomitantly higher yields and higher contents of oxygenated monoterpenoids and pulegone. Regarding the larvicidal activity, EO containing 95.18% of pulegone showed a LC50 of 69.05 µL L-1 against the Odonata larvae.Pesquisas sobre produtos derivados de plantas visando à descoberta de extrativos úteis para diferentes fins são crescentes e, dentre estes, destacam-se os óleos essenciais (OE), tendo como uma de suas principais fontes a família Lamiaceae. Pertencente a ela, Hesperozygis ringens (Benth.) Epling é uma espécie nativa do bioma Pampa, conhecida popularmente por espanta-pulga. O presente trabalho teve como objetivos avaliar o potencial biológico do OE de suas folhas como alelopático e larvicida, bem como verificar as variabilidades sazonal e individual do rendimento e composição química desse óleo. O OE de quatro indivíduos foi obtido de folhas coletadas nas diferentes estações de um ano, determinando-se o rendimento do OE (% m/m) e as composições químicas das amostras. O OE foi extraído por hidrodestilação, em aparelho Clevenger e teve sua composição química analisada por cromatografia gasosa acoplada à espectrometria de massas (CG-EM) e cromatografia gasosa com detector de ionização em chama (CG-DIC). O potencial alelopático do OE foi testado em sementes de Dicotiledôneas e Monocotiledôneas, classificadas como bioindicadoras [Avena strigosa Schreb. e Lactuca sativa L.], plantas daninhas [Lolium multiflorum Lam. e Bidens pilosa L.] e culturas [Oryza sativa L. e Glycine max (L.) Merr.], através da avaliação das seguintes variáveis: germinação, índice de velocidade de emergência e comprimentos das raízes, partes aéreas e total das plântulas. As concentrações testadas foram: 0,25; 0,50; 1,00; 2,50; 5,00%. O OE de H. ringens também teve sua atividade larvicida testada em larvas de Odonata, a fim de determinar sua concentração letal 50% (CL50). Em relação aos testes alelopáticos sobre Dicotiledôneas, o OE com 82,02% de pulegona inibiu completamente a germinação de B. pilosa na concentração de 5,00%, sem afetar significativamente L. sativa, porém retardando a emergência e prejudicando os comprimentos de raiz e plântula de G. max. Em relação às monocotiledôneas, a mesma concentração prejudicou fortemente L. multiflorum, sem afetar a germinação de O. sativa, porém influenciando negativamente os demais parâmetros. Já o desenvolvimento das sementes de A. strigosa foi completamente inibido na concentração de 2,50%. O rendimento do OE demonstrou ser influenciado pela sazonalidade, sendo maior na primavera, verão e outono. O rendimento também apresentou variabilidade individual (1,15 a 4,38%), demonstrando ser mais elevado no indivíduo 4. Em relação à composição química, os OE obtidos de folhas coletadas no outono, primavera e verão apresentaram resultados semelhantes. A produção do constituinte majoritário (pulegona) e da classe predominante (monoterpenoides oxigenados) foram favorecidos naprimavera, verão e outono. Estas estações demonstraram ser as mais favoráveis para extração de OE por apresentar concomitantemente maiores rendimentos e teores mais elevados de monoterpenoides oxigenados e de pulegona. Quanto à atividade larvicida, o OE contendo 95,18% de pulegona apresentou uma CL50 de 69,05 µL L-1 frente a larvas de Odonata.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Engenharia FlorestalUFSMBRRecursos Florestais e Engenharia FlorestalLamiaceaeAlelopatiaSazonalidadeLarvicidaPulegonaAllelopathySeasonalityLarvicidalPulegoneCNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTALÓleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicasEssential oil of Hesperozygis ringens (Benth.) Epling: variability of yield, chemical composition and biological activitiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisHeinzmann, Berta Mariahttp://lattes.cnpq.br/0786124562427815Barros, Francisco Maikon Corrêa dehttp://lattes.cnpq.br/3640649127516310Reiniger, Lia Rejane Silveirahttp://lattes.cnpq.br/5739294882585391http://lattes.cnpq.br/0255167092609424Pinheiro, Carlos Garrido500200000003400500300500500ef52d431-0698-40fb-ab56-1dbd415cc5d7466e61fc-ff46-47fd-b0ca-9a6a4d61376973eee66e-f2d5-4cfa-a3c6-ec782eb7e5e43cd76448-fb79-4eef-964a-72648182483cinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALPINHEIRO, CARLOS GARRIDO.pdfapplication/pdf2787458http://repositorio.ufsm.br/bitstream/1/8744/1/PINHEIRO%2c%20CARLOS%20GARRIDO.pdf1e312d8cd714f3c15510c5feb5503b41MD51TEXTPINHEIRO, CARLOS GARRIDO.pdf.txtPINHEIRO, CARLOS GARRIDO.pdf.txtExtracted texttext/plain173103http://repositorio.ufsm.br/bitstream/1/8744/2/PINHEIRO%2c%20CARLOS%20GARRIDO.pdf.txte03ecd7da16c99df64a3fa4b1e40f7d9MD52THUMBNAILPINHEIRO, CARLOS GARRIDO.pdf.jpgPINHEIRO, CARLOS GARRIDO.pdf.jpgIM Thumbnailimage/jpeg4787http://repositorio.ufsm.br/bitstream/1/8744/3/PINHEIRO%2c%20CARLOS%20GARRIDO.pdf.jpg29173bbebda797481006823ce8bd8e2dMD531/87442022-09-02 13:58:10.351oai:repositorio.ufsm.br:1/8744Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-09-02T16:58:10Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Óleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicas |
dc.title.alternative.eng.fl_str_mv |
Essential oil of Hesperozygis ringens (Benth.) Epling: variability of yield, chemical composition and biological activities |
title |
Óleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicas |
spellingShingle |
Óleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicas Pinheiro, Carlos Garrido Lamiaceae Alelopatia Sazonalidade Larvicida Pulegona Allelopathy Seasonality Larvicidal Pulegone CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
title_short |
Óleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicas |
title_full |
Óleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicas |
title_fullStr |
Óleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicas |
title_full_unstemmed |
Óleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicas |
title_sort |
Óleo essencial de Hesperozygis ringens (Benth.) Epling: variabilidade do rendimento, composição química e atividades biológicas |
author |
Pinheiro, Carlos Garrido |
author_facet |
Pinheiro, Carlos Garrido |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Heinzmann, Berta Maria |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0786124562427815 |
dc.contributor.referee1.fl_str_mv |
Barros, Francisco Maikon Corrêa de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3640649127516310 |
dc.contributor.referee2.fl_str_mv |
Reiniger, Lia Rejane Silveira |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5739294882585391 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0255167092609424 |
dc.contributor.author.fl_str_mv |
Pinheiro, Carlos Garrido |
contributor_str_mv |
Heinzmann, Berta Maria Barros, Francisco Maikon Corrêa de Reiniger, Lia Rejane Silveira |
dc.subject.por.fl_str_mv |
Lamiaceae Alelopatia Sazonalidade Larvicida Pulegona |
topic |
Lamiaceae Alelopatia Sazonalidade Larvicida Pulegona Allelopathy Seasonality Larvicidal Pulegone CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
dc.subject.eng.fl_str_mv |
Allelopathy Seasonality Larvicidal Pulegone |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL |
description |
Researches involving plant derived products aiming to discover extractives useful for different purposes have been growing, and among them, essential oils (EO) are noteworthy. One of the main sources of EO is the Lamiaceae family. Belonging to this family, Hesperozygis ringens (Benth.) Epling is a native species from the Pampa biome, popularly known as espanta-pulga . This study aimed to evaluate the biological potential of EO from its leaves as allelopathic and larvicidal, as well as to check the seasonal and individual variability of yield and chemical composition of this oil. EO of four specimens were obtained from leaves collected in different seasons of a year and had their yield (% m/m) and chemical compositions determined. EO were extracted by hidrodestillation in Clevenger apparatus and analyzed by gas chromatography coupled to mass spectrometry (GC-MS) and gas chromatography with flame ionization detector (GC-FID). The allelopathic potential of the EO was tested against seeds of Dicots and Monocots classified as bioindicators [Avena strigosa Schreb. and Lactuca sativa L.], weeds [Lolium multiflorum Lam and Bidens pilosa L.] and crops [Oryza sativa L. and Glycine max (L.) Merr.] by observing the following variables: germination, emergence velocity index, root length, shoot lenght and total length of seedlings. The tested concentrations were 0.25, 0.50, 1.00, 2.50 and 5.00%.The EO of H. ringens had also its larvicidal activity tested against larvae of Odonata in order to determine its 50% lethal concentration (LC50). Regarding the allelopathic tests on Dicotyledons, EO with 82.02% of pulegone inhibited completely the germination of B. pilosa at 5.00%, without affecting significantly L. sativa, but slowing the emergence and damaging root lengths and seedlings of G. max. Regarding the monocots, the same concentration strongly impaired L. multiflorum, without affecting the germination of O. sativa, but it influenced negatively the other evaluated parameters. In addition, the development of the seeds of A. strigosa was completely inhibited at the concentration of 2.50%. The yield of EO proved to be influenced by seasonality, being higher in spring, summer, and autumn. The yield also showed individual variability (1.15 a 4.38%), proving to be higher in specimen 4. The chemical composition of EO obtained from leaves collected in autumn, spring, and summer showed similar results. The production of the major constituent (pulegone) and the predominant class (oxygenated monoterpenoids) were favored in spring, summer, and autumn. These seasons proved to be more indicated for the EO extraction, since they enabled concomitantly higher yields and higher contents of oxygenated monoterpenoids and pulegone. Regarding the larvicidal activity, EO containing 95.18% of pulegone showed a LC50 of 69.05 µL L-1 against the Odonata larvae. |
publishDate |
2014 |
dc.date.accessioned.fl_str_mv |
2014-12-01 |
dc.date.available.fl_str_mv |
2014-12-01 |
dc.date.issued.fl_str_mv |
2014-02-25 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
PINHEIRO, Carlos Garrido. Essential oil of Hesperozygis ringens (Benth.) Epling: variability of yield, chemical composition and biological activities. 2014. 91 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/8744 |
identifier_str_mv |
PINHEIRO, Carlos Garrido. Essential oil of Hesperozygis ringens (Benth.) Epling: variability of yield, chemical composition and biological activities. 2014. 91 f. Dissertação (Mestrado em Recursos Florestais e Engenharia Florestal) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
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http://repositorio.ufsm.br/handle/1/8744 |
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Universidade Federal de Santa Maria |
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UFSM |
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Recursos Florestais e Engenharia Florestal |
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