Susceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/8963 |
Resumo: | Malassezia pachydermatis is an opportunistic fungus associated to dermatomycoses and otopathies in domestic and wild animals. The aim of this study was to compare the susceptibility profile of clinical isolates of M. pachydermatis against topic and systemic antifungals, through two standardized CLSI techniques: broth microdilution (M27-A3, 2008) and disk diffusion (M44-A, 2004). A standard Candida albicans strain (ATCC 28367) was used as quality control. M. pachydermatis isolates assayed through the broth microdilution method showed susceptibility to anfotericina-B (100%), fluconazole (97,83%), ketoconazole (95.66%), itraconazole (93,48%), followed by clotrimazole and miconazole (86.96%). The disk diffusion method showed susceptibility of 97,83% to nystatin, 95,66% to itraconazole and to amphotericin B, 91,32% to ketoconazole, 89,14% to fluconazole and of 86.96% to miconazole and clotrimazole. Through the in vitro induction of resistance to fluconazole (100%), cross-resistance was observed through the broth microdilution method among the 30 M. pachydermatis isolates against the azoles itraconazole (93%), ketoconazole (97%) and voriconazole (100%). The study of the activities of the essential oils obtained through the broth microdilution, based on geometric means of the minimum fungicidal concentration, showed that concentrations above 195.42 μg/mL, 332.49 μg/mL and 448.8 μg/mL of oregano, Mexican oregano and cinnamon essential oils, respectively, have fungicidal action against M. pachydermatis, independently of the sensitivity or resistance to fluconazole. The fungicidal activity of the essential oils against fluconazole-resistant M. pachydermatis strains is important for further therapeutic studies of this mycosis. Despite the susceptibility observed among sensitive M. pachydermatis strains suggesting fluconazole as a safe drug for the treatment of most cases of superficial and invasive malasseziosis, it is essential continuous surveillance studies to detect changes in the microbiological profile due to therapeutic practices. |
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2013-05-092013-05-092011-02-01JESUS, Francielli Pantella Kunz de. SUSCEPTIBILITY OF FLUCONAZOLE SENSITIVE AND FLUCONAZOLE RESISTANT Malassezia pachydermatis ISOLATES AGAINST ANTIFUNGALS AND ESSENTIAL OILS. 2011. 115 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/8963Malassezia pachydermatis is an opportunistic fungus associated to dermatomycoses and otopathies in domestic and wild animals. The aim of this study was to compare the susceptibility profile of clinical isolates of M. pachydermatis against topic and systemic antifungals, through two standardized CLSI techniques: broth microdilution (M27-A3, 2008) and disk diffusion (M44-A, 2004). A standard Candida albicans strain (ATCC 28367) was used as quality control. M. pachydermatis isolates assayed through the broth microdilution method showed susceptibility to anfotericina-B (100%), fluconazole (97,83%), ketoconazole (95.66%), itraconazole (93,48%), followed by clotrimazole and miconazole (86.96%). The disk diffusion method showed susceptibility of 97,83% to nystatin, 95,66% to itraconazole and to amphotericin B, 91,32% to ketoconazole, 89,14% to fluconazole and of 86.96% to miconazole and clotrimazole. Through the in vitro induction of resistance to fluconazole (100%), cross-resistance was observed through the broth microdilution method among the 30 M. pachydermatis isolates against the azoles itraconazole (93%), ketoconazole (97%) and voriconazole (100%). The study of the activities of the essential oils obtained through the broth microdilution, based on geometric means of the minimum fungicidal concentration, showed that concentrations above 195.42 μg/mL, 332.49 μg/mL and 448.8 μg/mL of oregano, Mexican oregano and cinnamon essential oils, respectively, have fungicidal action against M. pachydermatis, independently of the sensitivity or resistance to fluconazole. The fungicidal activity of the essential oils against fluconazole-resistant M. pachydermatis strains is important for further therapeutic studies of this mycosis. Despite the susceptibility observed among sensitive M. pachydermatis strains suggesting fluconazole as a safe drug for the treatment of most cases of superficial and invasive malasseziosis, it is essential continuous surveillance studies to detect changes in the microbiological profile due to therapeutic practices.Malassezia pachydermatis é um fungo oportunista, associado à dermatomicoses e otopatias em animais domésticos e selvagens. O objetivo deste estudo foi comparar o perfil de susceptibilidade de isolados clínicos de M. pachydermatis frente antifúngicos tópicos e sistêmicos, através de duas técnicas padronizadas pelo CLSI: microdiluição em caldo (M27- A3, 2008) e disco-difusão (M44-A, 2004). Para todos os testes foi utilizada uma cepa padrão de Candida albicans (ATCC 28367) como controle de qualidade. Isolados de M. pachydermatis testados através do método de microdiluição em caldo apresentaram em ordem decrescente de susceptibilidade: anfotericina-B (100%), fluconazol (97,83%), cetoconazol (95,66%), itraconazol (93.48%), seguido de clotrimazol e miconazol (86,96%). Pelo método de disco-difusão 97,83% dos isolados foram suscetíveis a nistatina, 95,66% ao itraconazol e a anfotericina-B; 91,32% ao cetoconazol, 89,14% ao fluconazol; e 86,96% ao miconazol e clotrimazol. Através da indução de resistência in vitro, de 30 isolados de M. pachydermatis, ao fluconazol (100%) foi possível evidenciar resistência cruzada pelo método de microdiluição em caldo frente aos antifúngicos azólicos, itraconazol (93%), cetoconazol (97%) e voriconazol (100%). O estudo da atividade de óleos essenciais gerados pelo método de microdiluição em caldo, baseado nas médias geométricas das CFMs, evidenciou-se que concentrações acima de 195.42 μg/mL de óleo essencial de orégano, 332.49 μg/mL de óleo essencial de orégano mexicano e 448.8 μg/mL de óleo essencial de canela, possuem ação fungicida in vitro sobre M. pachydermatis, independente da sensibilidade ou resistência ao fluconazol. A atividade de óleos essenciais frente M. pachydermatis fluconazol resistente, é um achado que consolida a importância destes para estudos futuros voltados a terapêutica desta micose. Apesar dos dados de susceptibilidade apresentados pelas cepas sensíveis de M. pachydermatis sugerirem que o fluconazol ainda é uma droga segura para terapêutica da maioria dos casos de malasseziose superficial ou invasiva, é fundamental que estudos de vigilância epidemiológica sejam realizados continuamente para evidenciar mudanças no perfil microbiológico em função de práticas terapêuticas.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em FarmacologiaUFSMBRFarmacologiaMalassezia pachydermatisSusceptibilidadeResistênciaAntifúngicosÓleos essenciaisDisco-difusãoMicrodiluição em caldoMalassezia pachydermatisResistanceAntifungalEssential oilsSusceptibilityDisk diffusionBroth microdilutionCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIASusceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais.Susceptibility of fluconazole sensitive and fluconazole resistant Malassezia pachydermatis isolates against antifungals and essential oilsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSanturio, Janio Moraishttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787502H6Pereira, Daniela Isabel Brayerhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784721P4Botton, Sonia de Avilahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795162U4Jesus, Francielli Pantella Kunz de20100000000040050030050050006a1b29c-fde0-4a69-8097-2a9d6a9d964921a41e5e-e589-49a2-9309-770e909602158cf0f648-751e-463b-8607-0f38cce0874c24806e8f-fdc9-4fdd-9936-d012e1f365bainfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALJESUS, FRANCIELLI PANTELLA KUNZ DE.pdfapplication/pdf473466http://repositorio.ufsm.br/bitstream/1/8963/1/JESUS%2c%20FRANCIELLI%20PANTELLA%20%20KUNZ%20DE.pdf73eafea9986fa10eb9e6685e3392d808MD51TEXTJESUS, FRANCIELLI PANTELLA KUNZ DE.pdf.txtJESUS, FRANCIELLI PANTELLA KUNZ DE.pdf.txtExtracted texttext/plain201440http://repositorio.ufsm.br/bitstream/1/8963/2/JESUS%2c%20FRANCIELLI%20PANTELLA%20%20KUNZ%20DE.pdf.txt899397ac13a5f59ffa892a9f8a052053MD52THUMBNAILJESUS, FRANCIELLI PANTELLA KUNZ DE.pdf.jpgJESUS, FRANCIELLI PANTELLA KUNZ DE.pdf.jpgIM Thumbnailimage/jpeg4884http://repositorio.ufsm.br/bitstream/1/8963/3/JESUS%2c%20FRANCIELLI%20PANTELLA%20%20KUNZ%20DE.pdf.jpgec148cc419d34e917b9cbe6a0d9b8659MD531/89632022-03-02 16:36:17.124oai:repositorio.ufsm.br:1/8963Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-03-02T19:36:17Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Susceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais. |
dc.title.alternative.eng.fl_str_mv |
Susceptibility of fluconazole sensitive and fluconazole resistant Malassezia pachydermatis isolates against antifungals and essential oils |
title |
Susceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais. |
spellingShingle |
Susceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais. Jesus, Francielli Pantella Kunz de Malassezia pachydermatis Susceptibilidade Resistência Antifúngicos Óleos essenciais Disco-difusão Microdiluição em caldo Malassezia pachydermatis Resistance Antifungal Essential oils Susceptibility Disk diffusion Broth microdilution CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Susceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais. |
title_full |
Susceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais. |
title_fullStr |
Susceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais. |
title_full_unstemmed |
Susceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais. |
title_sort |
Susceptibilidade de isolados de Malassezia pachydermatis sensíveis e resistentes ao fluconazol frente a antifúngicos e óleos essenciais. |
author |
Jesus, Francielli Pantella Kunz de |
author_facet |
Jesus, Francielli Pantella Kunz de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santurio, Janio Morais |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787502H6 |
dc.contributor.referee1.fl_str_mv |
Pereira, Daniela Isabel Brayer |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784721P4 |
dc.contributor.referee2.fl_str_mv |
Botton, Sonia de Avila |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795162U4 |
dc.contributor.author.fl_str_mv |
Jesus, Francielli Pantella Kunz de |
contributor_str_mv |
Santurio, Janio Morais Pereira, Daniela Isabel Brayer Botton, Sonia de Avila |
dc.subject.por.fl_str_mv |
Malassezia pachydermatis Susceptibilidade Resistência Antifúngicos Óleos essenciais Disco-difusão Microdiluição em caldo |
topic |
Malassezia pachydermatis Susceptibilidade Resistência Antifúngicos Óleos essenciais Disco-difusão Microdiluição em caldo Malassezia pachydermatis Resistance Antifungal Essential oils Susceptibility Disk diffusion Broth microdilution CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
dc.subject.eng.fl_str_mv |
Malassezia pachydermatis Resistance Antifungal Essential oils Susceptibility Disk diffusion Broth microdilution |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Malassezia pachydermatis is an opportunistic fungus associated to dermatomycoses and otopathies in domestic and wild animals. The aim of this study was to compare the susceptibility profile of clinical isolates of M. pachydermatis against topic and systemic antifungals, through two standardized CLSI techniques: broth microdilution (M27-A3, 2008) and disk diffusion (M44-A, 2004). A standard Candida albicans strain (ATCC 28367) was used as quality control. M. pachydermatis isolates assayed through the broth microdilution method showed susceptibility to anfotericina-B (100%), fluconazole (97,83%), ketoconazole (95.66%), itraconazole (93,48%), followed by clotrimazole and miconazole (86.96%). The disk diffusion method showed susceptibility of 97,83% to nystatin, 95,66% to itraconazole and to amphotericin B, 91,32% to ketoconazole, 89,14% to fluconazole and of 86.96% to miconazole and clotrimazole. Through the in vitro induction of resistance to fluconazole (100%), cross-resistance was observed through the broth microdilution method among the 30 M. pachydermatis isolates against the azoles itraconazole (93%), ketoconazole (97%) and voriconazole (100%). The study of the activities of the essential oils obtained through the broth microdilution, based on geometric means of the minimum fungicidal concentration, showed that concentrations above 195.42 μg/mL, 332.49 μg/mL and 448.8 μg/mL of oregano, Mexican oregano and cinnamon essential oils, respectively, have fungicidal action against M. pachydermatis, independently of the sensitivity or resistance to fluconazole. The fungicidal activity of the essential oils against fluconazole-resistant M. pachydermatis strains is important for further therapeutic studies of this mycosis. Despite the susceptibility observed among sensitive M. pachydermatis strains suggesting fluconazole as a safe drug for the treatment of most cases of superficial and invasive malasseziosis, it is essential continuous surveillance studies to detect changes in the microbiological profile due to therapeutic practices. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-01 |
dc.date.accessioned.fl_str_mv |
2013-05-09 |
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2013-05-09 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
JESUS, Francielli Pantella Kunz de. SUSCEPTIBILITY OF FLUCONAZOLE SENSITIVE AND FLUCONAZOLE RESISTANT Malassezia pachydermatis ISOLATES AGAINST ANTIFUNGALS AND ESSENTIAL OILS. 2011. 115 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/8963 |
identifier_str_mv |
JESUS, Francielli Pantella Kunz de. SUSCEPTIBILITY OF FLUCONAZOLE SENSITIVE AND FLUCONAZOLE RESISTANT Malassezia pachydermatis ISOLATES AGAINST ANTIFUNGALS AND ESSENTIAL OILS. 2011. 115 f. Dissertação (Mestrado em Farmácia) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
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http://repositorio.ufsm.br/handle/1/8963 |
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Universidade Federal de Santa Maria |
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Farmacologia |
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