Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/6485 |
Resumo: | Brazil is among the world's largest consumers of pesticides and their increased use is given in agriculture, especially monoculture in large areas. Research shows that the ototoxic agents, in addition to compromising the peripheral auditory and vestybular systems, they may cause changes in central auditory pathways. O objetivo deste estudo foi verificar os aspectos ultra-estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba, utilizando doses comprovadamente ototóxicas, lesivas às células ciliadas externas, avaliando-se as alterações anatômicas através da microscopia eletrônica de superfície. The aim of this study was to evaluate the ultrastructural aspects of the cochlea of guinea pigs exposed to pesticides and ginkgo biloba, using ototoxic doses, damaging the outer hair cells, to evaluate the anatomic changes by surface electron microscopy. This is a prospective experimental study, conducted in guinea pigs, wich the inclusion criterion was the presence of DPOAE. The sample was divided into five groups, in which they administered 0.9% saline solution (group 1 - control), pesticides - 0.3 mg / kg / day (group 2), Ginkgo biloba - 100mg/kg/day, 90 minutes after a pesticide 0.3 mg / kg / day (group 3), pesticides - 3 mg / kg / day (group 4) and Ginkgo biloba - 100mg/kg/day, 90 minutes after a pesticide 3.0 mg / kg / days (group 5) for seven consecutive days. The pesticide used was that of methamidophos from Fersol ®. The anatomical evaluation was performed with scanning electronic microscopy. The guinea pigs subjected to pesticides showed morphological cochlear lesions in the three turns analyzed by electronic microscopy, intensified according to the dosage received from the pesticide agent. Guinea pigs treated with pesticides and Ginkgo biloba showed a maintenance of ciliary architecture in outer hair cells in all cochlear turns, whereas in the group treated only with pesticides, there was disappearance of the cilia of outer hair cells and distortion in the architecture of cilia remaining the MoU. We conclude that the greater the exposure to pesticides increased the damage in outer hair cells of the cochlea of albino guinea pigs, while those who had previously received ginkgo biloba keep outer hair cells cytoarchitecture preserved. |
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Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo bilobaUltrastructural aspects in the guinea pig inner ear exposed to pesticide and ginkgo biloba.OtotoxicidadeAgrotóxicoOtoproteçãoGinkgo bilobaOtotoxicityPesticideOtoprotectionGinkgo bilobaCNPQ::CIENCIAS DA SAUDE::FONOAUDIOLOGIABrazil is among the world's largest consumers of pesticides and their increased use is given in agriculture, especially monoculture in large areas. Research shows that the ototoxic agents, in addition to compromising the peripheral auditory and vestybular systems, they may cause changes in central auditory pathways. O objetivo deste estudo foi verificar os aspectos ultra-estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba, utilizando doses comprovadamente ototóxicas, lesivas às células ciliadas externas, avaliando-se as alterações anatômicas através da microscopia eletrônica de superfície. The aim of this study was to evaluate the ultrastructural aspects of the cochlea of guinea pigs exposed to pesticides and ginkgo biloba, using ototoxic doses, damaging the outer hair cells, to evaluate the anatomic changes by surface electron microscopy. This is a prospective experimental study, conducted in guinea pigs, wich the inclusion criterion was the presence of DPOAE. The sample was divided into five groups, in which they administered 0.9% saline solution (group 1 - control), pesticides - 0.3 mg / kg / day (group 2), Ginkgo biloba - 100mg/kg/day, 90 minutes after a pesticide 0.3 mg / kg / day (group 3), pesticides - 3 mg / kg / day (group 4) and Ginkgo biloba - 100mg/kg/day, 90 minutes after a pesticide 3.0 mg / kg / days (group 5) for seven consecutive days. The pesticide used was that of methamidophos from Fersol ®. The anatomical evaluation was performed with scanning electronic microscopy. The guinea pigs subjected to pesticides showed morphological cochlear lesions in the three turns analyzed by electronic microscopy, intensified according to the dosage received from the pesticide agent. Guinea pigs treated with pesticides and Ginkgo biloba showed a maintenance of ciliary architecture in outer hair cells in all cochlear turns, whereas in the group treated only with pesticides, there was disappearance of the cilia of outer hair cells and distortion in the architecture of cilia remaining the MoU. We conclude that the greater the exposure to pesticides increased the damage in outer hair cells of the cochlea of albino guinea pigs, while those who had previously received ginkgo biloba keep outer hair cells cytoarchitecture preserved.O Brasil está entre os principais consumidores mundiais de agrotóxicos e sua maior utilização se dá na agricultura, especialmente na monocultura, em grandes extensões. Pesquisas demonstram que os agentes ototóxicos, além de comprometer os sistemas auditivo e vestibular periféricos, provocam ainda alterações nas vias auditivas centrais. O objetivo deste estudo foi verificar os aspectos ultra-estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba, utilizando doses comprovadamente ototóxicas, lesivas às células ciliadas externas, avaliando-se as alterações anatômicas através da microscopia eletrônica de superfície. Trata-se de um estudo experimental prospectivo, realizado em cobaias albinas, cujo critério de inclusão foi à presença de OEAPD. A amostra foi dividida em cinco grupos, nos quais se administrou soro fisiológico 0,9% (grupo 1 - controle), agrotóxico - 0,3mg/Kg/dia (grupo 2), Ginkgo biloba - 100mg/kg/dia, 90 minutos após, agrotóxico 0,3 mg/Kg/dia (grupo 3), agrotóxico 3 mg/Kg/dia (grupo 4) e Ginkgo biloba - 100mg/kg/dia, 90 minutos após, agrotóxico 3,0 mg/Kg/dia (grupo 5) durante sete dias consecutivos. O agrotóxico utilizado foi o metamidofós da Fersol®. A avaliação anatômica foi realizada com Microscopia Eletrônica de Varredura. As cobaias submetidas ao agrotóxico apresentaram alterações morfológicas cocleares, com lesões nas três espiras analisadas na microscopia eletrônica, intensificadas de acordo com a dosagem recebida do agente. As cobaias tratadas com agrotóxico e Ginkgo biloba, apresentaram uma manutenção da arquitetura ciliar nas células ciliadas externas em todas as espiras da cóclea, enquanto que nos grupo tratado somente com agrotóxico, houve desaparecimento dos cílios das células ciliadas externas e distorção na arquitetura dos cílios remanescentes à ME. Concluímos que quanto maior a exposição ao agrotóxico maior o dano nas CCE da cóclea de cobaias albinas, enquanto que aquelas que recebem previamente o ginkgo biloba mantêm a citoarquitetura das CCE preservadas.Universidade Federal de Santa MariaBRFonoaudiologiaUFSMPrograma de Pós-Graduação em Distúrbios da Comunicação HumanaSilveira, Aron Ferreira dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705548P6Bortholuzzi, Sônia Maria FigheraRossi, ângela Garciahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4708676D3Finckler, Andréa Dulor2010-10-132010-10-132010-07-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfFINCKLER, Andréa Dulor. ULTRASTRUCTURAL ASPECTS IN THE GUINEA PIG INNER EAR EXPOSED TO PESTICIDE AND GINKGO BILOBA.. 2010. 52 f. Dissertação (Mestrado em Fonoaudiologia) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/6485porinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-03-15T18:17:12Zoai:repositorio.ufsm.br:1/6485Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-03-15T18:17:12Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba Ultrastructural aspects in the guinea pig inner ear exposed to pesticide and ginkgo biloba. |
title |
Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba |
spellingShingle |
Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba Finckler, Andréa Dulor Ototoxicidade Agrotóxico Otoproteção Ginkgo biloba Ototoxicity Pesticide Otoprotection Ginkgo biloba CNPQ::CIENCIAS DA SAUDE::FONOAUDIOLOGIA |
title_short |
Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba |
title_full |
Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba |
title_fullStr |
Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba |
title_full_unstemmed |
Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba |
title_sort |
Aspectos ultra estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba |
author |
Finckler, Andréa Dulor |
author_facet |
Finckler, Andréa Dulor |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silveira, Aron Ferreira da http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705548P6 Bortholuzzi, Sônia Maria Fighera Rossi, ângela Garcia http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4708676D3 |
dc.contributor.author.fl_str_mv |
Finckler, Andréa Dulor |
dc.subject.por.fl_str_mv |
Ototoxicidade Agrotóxico Otoproteção Ginkgo biloba Ototoxicity Pesticide Otoprotection Ginkgo biloba CNPQ::CIENCIAS DA SAUDE::FONOAUDIOLOGIA |
topic |
Ototoxicidade Agrotóxico Otoproteção Ginkgo biloba Ototoxicity Pesticide Otoprotection Ginkgo biloba CNPQ::CIENCIAS DA SAUDE::FONOAUDIOLOGIA |
description |
Brazil is among the world's largest consumers of pesticides and their increased use is given in agriculture, especially monoculture in large areas. Research shows that the ototoxic agents, in addition to compromising the peripheral auditory and vestybular systems, they may cause changes in central auditory pathways. O objetivo deste estudo foi verificar os aspectos ultra-estruturais de cócleas de cobaias expostas a agrotóxico e ginkgo biloba, utilizando doses comprovadamente ototóxicas, lesivas às células ciliadas externas, avaliando-se as alterações anatômicas através da microscopia eletrônica de superfície. The aim of this study was to evaluate the ultrastructural aspects of the cochlea of guinea pigs exposed to pesticides and ginkgo biloba, using ototoxic doses, damaging the outer hair cells, to evaluate the anatomic changes by surface electron microscopy. This is a prospective experimental study, conducted in guinea pigs, wich the inclusion criterion was the presence of DPOAE. The sample was divided into five groups, in which they administered 0.9% saline solution (group 1 - control), pesticides - 0.3 mg / kg / day (group 2), Ginkgo biloba - 100mg/kg/day, 90 minutes after a pesticide 0.3 mg / kg / day (group 3), pesticides - 3 mg / kg / day (group 4) and Ginkgo biloba - 100mg/kg/day, 90 minutes after a pesticide 3.0 mg / kg / days (group 5) for seven consecutive days. The pesticide used was that of methamidophos from Fersol ®. The anatomical evaluation was performed with scanning electronic microscopy. The guinea pigs subjected to pesticides showed morphological cochlear lesions in the three turns analyzed by electronic microscopy, intensified according to the dosage received from the pesticide agent. Guinea pigs treated with pesticides and Ginkgo biloba showed a maintenance of ciliary architecture in outer hair cells in all cochlear turns, whereas in the group treated only with pesticides, there was disappearance of the cilia of outer hair cells and distortion in the architecture of cilia remaining the MoU. We conclude that the greater the exposure to pesticides increased the damage in outer hair cells of the cochlea of albino guinea pigs, while those who had previously received ginkgo biloba keep outer hair cells cytoarchitecture preserved. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-13 2010-10-13 2010-07-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
FINCKLER, Andréa Dulor. ULTRASTRUCTURAL ASPECTS IN THE GUINEA PIG INNER EAR EXPOSED TO PESTICIDE AND GINKGO BILOBA.. 2010. 52 f. Dissertação (Mestrado em Fonoaudiologia) - Universidade Federal de Santa Maria, Santa Maria, 2010. http://repositorio.ufsm.br/handle/1/6485 |
identifier_str_mv |
FINCKLER, Andréa Dulor. ULTRASTRUCTURAL ASPECTS IN THE GUINEA PIG INNER EAR EXPOSED TO PESTICIDE AND GINKGO BILOBA.. 2010. 52 f. Dissertação (Mestrado em Fonoaudiologia) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
url |
http://repositorio.ufsm.br/handle/1/6485 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Fonoaudiologia UFSM Programa de Pós-Graduação em Distúrbios da Comunicação Humana |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria BR Fonoaudiologia UFSM Programa de Pós-Graduação em Distúrbios da Comunicação Humana |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922108717400064 |