Lacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/22076 |
Resumo: | Nucleotide excision repair (NER) is the most versatile DNA repair pathway as it removes different kinds of bulky lesions. Due to its essential role for genome integrity, it appeared early in the evolution of species. However, most published studies are focused on humans, mice, yeast or bacteria. Considering the large amount of information on genome databases, it is currently possible to retrieve sequences from NER components in many organisms. Therefore, we attempted to characterize the potential orthologs of 10 critical components of the human NER pathway in 12 eukaryotic species by using similarity and structural criteria through the use of bioinformatical tools. This approach has allowed us to characterize gene and protein structures comparatively, taking a glance at some evolutionary aspects of the NER pathway. We obtained significant search results for the majority of the proteins in most of the organisms studied, mainly for factors that play a pivotal role in the pathway. However, we revisited significant differences and found new aspects that may imply a distinct functioning of this pathway in different organisms. Through the demonstration of the heterogeneity of the gene structures and a variety in the protein architecture of the NER components evaluated, our results highlight important differences between human NER and evolutionarily distant eukaryotes. |
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2021-08-26T17:58:39Z2021-08-26T17:58:39Z2020-10-21http://repositorio.ufsm.br/handle/1/22076Nucleotide excision repair (NER) is the most versatile DNA repair pathway as it removes different kinds of bulky lesions. Due to its essential role for genome integrity, it appeared early in the evolution of species. However, most published studies are focused on humans, mice, yeast or bacteria. Considering the large amount of information on genome databases, it is currently possible to retrieve sequences from NER components in many organisms. Therefore, we attempted to characterize the potential orthologs of 10 critical components of the human NER pathway in 12 eukaryotic species by using similarity and structural criteria through the use of bioinformatical tools. This approach has allowed us to characterize gene and protein structures comparatively, taking a glance at some evolutionary aspects of the NER pathway. We obtained significant search results for the majority of the proteins in most of the organisms studied, mainly for factors that play a pivotal role in the pathway. However, we revisited significant differences and found new aspects that may imply a distinct functioning of this pathway in different organisms. Through the demonstration of the heterogeneity of the gene structures and a variety in the protein architecture of the NER components evaluated, our results highlight important differences between human NER and evolutionarily distant eukaryotes.A via de reparo por excisão de nucleotídeos (NER) é o mais versátil mecanismo de reparo de DNA, já que está envolvido na remoção de diferentes tipos de lesões que causam grandes distorções na dupla-hélice. Devido à sua grande importância na manutenção da integridade genômica, essa via apareceu cedo na evolução das espécies. Além disso, a maioria dos estudos relacionados ao NER está focada em humanos, camundongos, leveduras e bactérias. Considerando a grande quantidade de dados disponíveis em bancos de dados genômicos, é possível obter sequências de componentes do NER em diferentes organismos. Dessa forma, visamos caracterizar potenciais ortólogos de componentes-chave da via NER em organismos eucarióticos usando diferentes critérios estruturais e de similaridade, através do uso de ferramentas de bioinformática. Essa metodologia nos permitiu caracterizar a estrutura de genes e proteínas de maneira comparativa, bem como esclarecer alguns aspectos evolutivos da via NER. Diante disso, foram obtidos resultados de busca significativos para a maioria das proteínas em grande parte dos organismos analisados, principalmente para aquelas que têm um papel essencial na via. Entretanto, reanalisamos importantes diferenças e encontramos novos aspectos que podem implicar um funcionamento distinto do NER em diferentes organismos. Através da demonstração da heterogeneidade das estruturas gênicas e da variedade na arquitetura das proteínas dessa via, nossos resultados revelam diferenças importantes entre o NER humano e o de eucariotos evolutivamente distantes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessReparo de DNAVia NEREucariotosEstrutura gênicaArquitetura de domíniosDNA repairNER pathwayEukaryotesGene structureDomain architectureCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICALacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotosOpen gaps in the evolution of the eukaryotic nucleotide excision repairinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSchuch, André Passagliahttp://lattes.cnpq.br/4932611269622766Segatto, Ana Lúcia AnversaMenck, Carlos Frederico MartinsLoreto, Elgion Lucio da Silvahttp://lattes.cnpq.br/6281810289889181Feltrin, Rayana dos Santos200800000002600600600600600600d93539a4-7b55-44e5-9c20-52601682b4f70d165aaf-468f-4c81-9536-7401f2fcd2ed5ab3e05d-9397-4567-b75b-9751336f662fe9b02cbc-3861-426b-bb43-fb3b5c4151f8ab41f5b8-3dea-4caa-86ab-c37a1044a379reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGCBBT_2020_FELTRIN_RAYANA.pdfDIS_PPGCBBT_2020_FELTRIN_RAYANA.pdfDissertaçãoapplication/pdf11732417http://repositorio.ufsm.br/bitstream/1/22076/1/DIS_PPGCBBT_2020_FELTRIN_RAYANA.pdfb5df814977ed41ece327d94ec2cbb286MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Lacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotos |
dc.title.alternative.eng.fl_str_mv |
Open gaps in the evolution of the eukaryotic nucleotide excision repair |
title |
Lacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotos |
spellingShingle |
Lacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotos Feltrin, Rayana dos Santos Reparo de DNA Via NER Eucariotos Estrutura gênica Arquitetura de domínios DNA repair NER pathway Eukaryotes Gene structure Domain architecture CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Lacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotos |
title_full |
Lacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotos |
title_fullStr |
Lacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotos |
title_full_unstemmed |
Lacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotos |
title_sort |
Lacunas na evolução da via de reparo por excisão de nucleotídeos em eucariotos |
author |
Feltrin, Rayana dos Santos |
author_facet |
Feltrin, Rayana dos Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Schuch, André Passaglia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4932611269622766 |
dc.contributor.advisor-co1.fl_str_mv |
Segatto, Ana Lúcia Anversa |
dc.contributor.referee1.fl_str_mv |
Menck, Carlos Frederico Martins |
dc.contributor.referee2.fl_str_mv |
Loreto, Elgion Lucio da Silva |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6281810289889181 |
dc.contributor.author.fl_str_mv |
Feltrin, Rayana dos Santos |
contributor_str_mv |
Schuch, André Passaglia Segatto, Ana Lúcia Anversa Menck, Carlos Frederico Martins Loreto, Elgion Lucio da Silva |
dc.subject.por.fl_str_mv |
Reparo de DNA Via NER Eucariotos Estrutura gênica Arquitetura de domínios |
topic |
Reparo de DNA Via NER Eucariotos Estrutura gênica Arquitetura de domínios DNA repair NER pathway Eukaryotes Gene structure Domain architecture CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
DNA repair NER pathway Eukaryotes Gene structure Domain architecture |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Nucleotide excision repair (NER) is the most versatile DNA repair pathway as it removes different kinds of bulky lesions. Due to its essential role for genome integrity, it appeared early in the evolution of species. However, most published studies are focused on humans, mice, yeast or bacteria. Considering the large amount of information on genome databases, it is currently possible to retrieve sequences from NER components in many organisms. Therefore, we attempted to characterize the potential orthologs of 10 critical components of the human NER pathway in 12 eukaryotic species by using similarity and structural criteria through the use of bioinformatical tools. This approach has allowed us to characterize gene and protein structures comparatively, taking a glance at some evolutionary aspects of the NER pathway. We obtained significant search results for the majority of the proteins in most of the organisms studied, mainly for factors that play a pivotal role in the pathway. However, we revisited significant differences and found new aspects that may imply a distinct functioning of this pathway in different organisms. Through the demonstration of the heterogeneity of the gene structures and a variety in the protein architecture of the NER components evaluated, our results highlight important differences between human NER and evolutionarily distant eukaryotes. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-10-21 |
dc.date.accessioned.fl_str_mv |
2021-08-26T17:58:39Z |
dc.date.available.fl_str_mv |
2021-08-26T17:58:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/22076 |
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http://repositorio.ufsm.br/handle/1/22076 |
dc.language.iso.fl_str_mv |
por |
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por |
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200800000002 |
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600 600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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