Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápido
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/20847 |
Resumo: | Rapidly Growing Mycobacteria (RGM) are emerging pathogens responsible for various human infections, although they are often considered only as contaminants or colonizers. The formation of biofilms by these microorganisms causes great concern for public health, due to its greater pathogenicity in humans and antimicrobial resistance. Microbial cells within the biofilm are protected by an autoproduced polymer matrix that delays the diffusion of antimicrobials, preventing the drug from penetrating the deeper layers and having its effective action. Thus, it is important to seek effective alternatives against the formation of biofilms. In this sense, the use of biological products as well as the use of nanostructures have been studied for the control of formation and development of biofilms. This work had as main objective to evaluate the action of the essential oil (EO) of Cymbopogon flexuosus free and in nanoemulsion on strains of RGM, in planktonic and sessile forms. EO was chemically characterized. The nanoemulsion obtained by the homogenization method under high stirring was characterized for its stability, surface charge and particle size. The assays of Minimum Inhibitory Concentration (MIC) and time-kill curve were determined against standard strains of RGM Mycobacterium abscessus (ATCC 19977), Mycobacterium fortuitum (ATCC 6841) and Mycobacterium massiliense (ATCC 48898). The ability of the oil and nanoemulsion to inhibit biofilm formation and to destroy it was evaluated by semi-quantitative macro technique. In the susceptibility test of the microorganisms in planktonic form, the nanoemulsion presented higher microbial activity, with MIC values lower than those presented by free EO. However, the free EO had bactericidal action, while the nanostructure had only bacteriostatic action. In the activity evaluation against RGM biofilms, both were efficient in the destruction of the biofilm already formed, while only the free oil inhibited the formation of mycobacterial biofilm. The results of this dissertation impact on the discovery of a new pharmacological formulation, capable of enhancing the effects of EO and impairing the formation of biofilms. In addition, this work stimulates the research in methodologies that aim to clarify the influence exerted by the EO and the nanoemulsion on the genetic and physiological factors associated with the biofilms formation. |
id |
UFSM_d283d99f1450c8a3aa520ed91bee3ff0 |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/20847 |
network_acronym_str |
UFSM |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
|
spelling |
Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápidoOil activity of free and nanoestructured Cymbopogon flexuosus in front of rapidly growing mycobacteriaMCRBiofilmesCapim-limãoNanoemulsãoRGMBiofilmsLemongrassNanoemulsionCNPQ::CIENCIAS DA SAUDE::FARMACIARapidly Growing Mycobacteria (RGM) are emerging pathogens responsible for various human infections, although they are often considered only as contaminants or colonizers. The formation of biofilms by these microorganisms causes great concern for public health, due to its greater pathogenicity in humans and antimicrobial resistance. Microbial cells within the biofilm are protected by an autoproduced polymer matrix that delays the diffusion of antimicrobials, preventing the drug from penetrating the deeper layers and having its effective action. Thus, it is important to seek effective alternatives against the formation of biofilms. In this sense, the use of biological products as well as the use of nanostructures have been studied for the control of formation and development of biofilms. This work had as main objective to evaluate the action of the essential oil (EO) of Cymbopogon flexuosus free and in nanoemulsion on strains of RGM, in planktonic and sessile forms. EO was chemically characterized. The nanoemulsion obtained by the homogenization method under high stirring was characterized for its stability, surface charge and particle size. The assays of Minimum Inhibitory Concentration (MIC) and time-kill curve were determined against standard strains of RGM Mycobacterium abscessus (ATCC 19977), Mycobacterium fortuitum (ATCC 6841) and Mycobacterium massiliense (ATCC 48898). The ability of the oil and nanoemulsion to inhibit biofilm formation and to destroy it was evaluated by semi-quantitative macro technique. In the susceptibility test of the microorganisms in planktonic form, the nanoemulsion presented higher microbial activity, with MIC values lower than those presented by free EO. However, the free EO had bactericidal action, while the nanostructure had only bacteriostatic action. In the activity evaluation against RGM biofilms, both were efficient in the destruction of the biofilm already formed, while only the free oil inhibited the formation of mycobacterial biofilm. The results of this dissertation impact on the discovery of a new pharmacological formulation, capable of enhancing the effects of EO and impairing the formation of biofilms. In addition, this work stimulates the research in methodologies that aim to clarify the influence exerted by the EO and the nanoemulsion on the genetic and physiological factors associated with the biofilms formation.As micobactérias de crescimento rápido (MCR) são patógenos emergentes responsáveis por diversas infecções em humanos, embora muitas vezes sejam considerados apenas como contaminantes ou colonizadores. A formação de biofilmes por esses micro-organismos causa grande preocupação para a saúde pública, devido sua maior patogenicidade em humanos e a resistência aos antimicrobianos. As células microbianas dentro do biofilme são protegidas por uma matriz polimérica autoproduzida que retarda a difusão dos antimicrobianos, impedindo que o fármaco penetre nas camadas mais profundas e tenha sua ação efetiva. Deste modo, torna-se importante buscar alternativas eficazes contra a formação de biofilmes. Neste sentido, o uso de produtos biológicos, bem como a utilização de nanoestruturas vêm sendo estudados para o controle de formação e desenvolvimento de biofilmes. Este trabalho teve como objetivo principal avaliar a ação do óleo essencial (OE) de Cymbopogon flexuosus livre e em nanoemulsão sobre cepas de MCR, na forma planctônica e séssil. O OE foi caracterizado quimicamente. A nanoemulsão obtida pelo método de homogeneização sob alta agitação foi caracterizada quanto a sua estabilidade, carga de superfície e tamanho de partícula. Os ensaios de Concentração Inibitória Mínima (CIM) e de curva de morte foram determinadas contra as cepas padrão de MCR Mycobacterium abscessus (ATCC 19977), Mycobacterium fortuitum (ATCC 6841) e Mycobacterium massiliense (ATCC 48898). A capacidade do óleo e da nanoemulsão em inibir a formação do biofilme e destrui-lo foi avaliada por macrotécnica semi-quantitativa. No ensaio de suscetibilidade dos micro-organismos na forma planctônica, a nanoemulsão apresentou maior atividade microbiana, com valores da CIM inferiores aos apresentados pelo OE livre. No entanto, o OE livre teve ação bactericida, enquanto a nanoestrutura teve somente ação bacteriostática. Na avaliação da atividade contra biofilmes de MCR, ambos foram eficientes na destruição do biofilme já formado, enquanto somente o óleo livre inibiu da formação de biofilme micobacteriano. Os resultados desta dissertação impactam sobre a descoberta de uma nova formulação famacêutica, capaz de potencializar os efeitos do OE e prejudicar a formação de biofilmes. Ademais, este trabalho estimula o aprofundamento em metodologias que visam esclarecer a influência exercida pelo OE e a nanoemulsão sobre os fatores genéticos e fisiológicos associados à formação de biofilmes.Universidade Federal de Santa MariaBrasilAnálises Clínicas e ToxicológicasUFSMPrograma de Pós-Graduação em Ciências FarmacêuticasCentro de Ciências da SaúdeCampos, Marli Matiko Anraku dehttp://lattes.cnpq.br/6421182991125434Santos, Roberto Christ ViannaPeres, Paulo Edelvar CorreaMachado, Alencar KolinskiRossi, Grazielle Guidolin2021-05-12T17:15:09Z2021-05-12T17:15:09Z2018-02-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/20847porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2022-10-06T19:54:39Zoai:repositorio.ufsm.br:1/20847Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-10-06T19:54:39Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápido Oil activity of free and nanoestructured Cymbopogon flexuosus in front of rapidly growing mycobacteria |
title |
Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápido |
spellingShingle |
Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápido Rossi, Grazielle Guidolin MCR Biofilmes Capim-limão Nanoemulsão RGM Biofilms Lemongrass Nanoemulsion CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápido |
title_full |
Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápido |
title_fullStr |
Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápido |
title_full_unstemmed |
Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápido |
title_sort |
Atividade do óleo de Cymbopogon flexuosus livre e nanoestruturado frente a micobactérias de crescimento rápido |
author |
Rossi, Grazielle Guidolin |
author_facet |
Rossi, Grazielle Guidolin |
author_role |
author |
dc.contributor.none.fl_str_mv |
Campos, Marli Matiko Anraku de http://lattes.cnpq.br/6421182991125434 Santos, Roberto Christ Vianna Peres, Paulo Edelvar Correa Machado, Alencar Kolinski |
dc.contributor.author.fl_str_mv |
Rossi, Grazielle Guidolin |
dc.subject.por.fl_str_mv |
MCR Biofilmes Capim-limão Nanoemulsão RGM Biofilms Lemongrass Nanoemulsion CNPQ::CIENCIAS DA SAUDE::FARMACIA |
topic |
MCR Biofilmes Capim-limão Nanoemulsão RGM Biofilms Lemongrass Nanoemulsion CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Rapidly Growing Mycobacteria (RGM) are emerging pathogens responsible for various human infections, although they are often considered only as contaminants or colonizers. The formation of biofilms by these microorganisms causes great concern for public health, due to its greater pathogenicity in humans and antimicrobial resistance. Microbial cells within the biofilm are protected by an autoproduced polymer matrix that delays the diffusion of antimicrobials, preventing the drug from penetrating the deeper layers and having its effective action. Thus, it is important to seek effective alternatives against the formation of biofilms. In this sense, the use of biological products as well as the use of nanostructures have been studied for the control of formation and development of biofilms. This work had as main objective to evaluate the action of the essential oil (EO) of Cymbopogon flexuosus free and in nanoemulsion on strains of RGM, in planktonic and sessile forms. EO was chemically characterized. The nanoemulsion obtained by the homogenization method under high stirring was characterized for its stability, surface charge and particle size. The assays of Minimum Inhibitory Concentration (MIC) and time-kill curve were determined against standard strains of RGM Mycobacterium abscessus (ATCC 19977), Mycobacterium fortuitum (ATCC 6841) and Mycobacterium massiliense (ATCC 48898). The ability of the oil and nanoemulsion to inhibit biofilm formation and to destroy it was evaluated by semi-quantitative macro technique. In the susceptibility test of the microorganisms in planktonic form, the nanoemulsion presented higher microbial activity, with MIC values lower than those presented by free EO. However, the free EO had bactericidal action, while the nanostructure had only bacteriostatic action. In the activity evaluation against RGM biofilms, both were efficient in the destruction of the biofilm already formed, while only the free oil inhibited the formation of mycobacterial biofilm. The results of this dissertation impact on the discovery of a new pharmacological formulation, capable of enhancing the effects of EO and impairing the formation of biofilms. In addition, this work stimulates the research in methodologies that aim to clarify the influence exerted by the EO and the nanoemulsion on the genetic and physiological factors associated with the biofilms formation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02-28 2021-05-12T17:15:09Z 2021-05-12T17:15:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/20847 |
url |
http://repositorio.ufsm.br/handle/1/20847 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Análises Clínicas e Toxicológicas UFSM Programa de Pós-Graduação em Ciências Farmacêuticas Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Análises Clínicas e Toxicológicas UFSM Programa de Pós-Graduação em Ciências Farmacêuticas Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922169113280512 |