Caracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/5059 |
Resumo: | The meiotic behavior and estimation of pollen viability were studied in seven cultivars of common bean (Phaseolus vulgaris L.), which were IAPAR 44, Guapo Brilhante, BRS Expedito, BRS Valente, Guateian 6662, Pérola e Iraí. Floral buds were collected from cultivars, fixed and stored under refrigeration for the preparation of slides by squashing technique anthers and coloring with acetic orceína 2% and reactive Alexander. It was tried to obtain the greatest possible number of cells of each stage of meiosis. The estimation of pollen viability was observed by counting 800 pollen grains per plant. To verify changes in size, were measured 100 grains of pollen of each cultivar considered mature. There were no cells with abnormalities during the analysis of meiotic behavior in all cultivars. The meiotic index were high and uniform over 90%, indicating that these cultivars have the meiotic process stable and use in plant breeding not present problems. The pollen grains showed no significant differences as to its size, being similar among cultivars. Data on meiotic behavior and pollen viability obtained in this work combined with the literature, emphasize that the bean has a meiotic process uniform, showing high potential fertility of male gametes, which facilitates the use of this species in breeding programs. |
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2017-05-082017-05-082012-02-17NORA, Gracieli Dalla. Cytogenetic characterization of common beans (Phaseolus vulgaris L.). 2012. 38 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/5059The meiotic behavior and estimation of pollen viability were studied in seven cultivars of common bean (Phaseolus vulgaris L.), which were IAPAR 44, Guapo Brilhante, BRS Expedito, BRS Valente, Guateian 6662, Pérola e Iraí. Floral buds were collected from cultivars, fixed and stored under refrigeration for the preparation of slides by squashing technique anthers and coloring with acetic orceína 2% and reactive Alexander. It was tried to obtain the greatest possible number of cells of each stage of meiosis. The estimation of pollen viability was observed by counting 800 pollen grains per plant. To verify changes in size, were measured 100 grains of pollen of each cultivar considered mature. There were no cells with abnormalities during the analysis of meiotic behavior in all cultivars. The meiotic index were high and uniform over 90%, indicating that these cultivars have the meiotic process stable and use in plant breeding not present problems. The pollen grains showed no significant differences as to its size, being similar among cultivars. Data on meiotic behavior and pollen viability obtained in this work combined with the literature, emphasize that the bean has a meiotic process uniform, showing high potential fertility of male gametes, which facilitates the use of this species in breeding programs.O comportamento meiótico e estimativa da viabilidade do pólen foram estudados em sete cultivares de feijão (Phaseolus vulgaris L.), sendo elas, IAPAR 44, Guapo Brilhante, BRS Expedito, BRS Valente, Guateian 6662, Pérola e Iraí. Botões florais foram coletados das cultivares, fixados e armazenados sob refrigeração para o preparo de lâminas pela técnica de esmagamento das anteras e coloração com orceína acética 2% e reativo de Alexander. Procurou-se obter o maior número de células possíveis de cada uma das fases da meiose. A estimativa da viabilidade polínica foi observada contando-se 800 grãos de pólen por planta. Para verificar alterações de tamanho, foram medidos 100 grãos de polens considerados maduros de cada cultivar. Não ocorreram células com anormalidades durante a análise do comportamento meiótico em todas as cultivares. Os índices meióticos encontrados foram elevados e uniformes, acima de 90%, indicando que estas cultivares possuem o processo meiótico estável e o uso em cruzamentos não apresentará problemas. Os grãos de polens não apresentaram diferenças significativas quanto ao seu tamanho, mostrando-se semelhante entre as cultivares. Os dados sobre comportamento meiótico e viabilidade polínica obtidos neste trabalho aliados aos da literatura, reforçam que o feijão possui um processo meiótico uniforme, demonstrando alto potencial de fertilidade dos gametas masculinos, o que facilita o uso dessa espécie em programas de melhoramento.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em AgronomiaUFSMBRAgronomiaCitogenéticaPhaseolus vulgaris L.Viabilidade polínicaCytogeneticPhaseolus vulgaris L.Pollen viabilityCNPQ::CIENCIAS AGRARIAS::AGRONOMIACaracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.)Cytogenetic characterization of common beans (Phaseolus vulgaris L.)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTedesco, Solange Bosiohttp://lattes.cnpq.br/2541303074261787Ribeiro, Nerinéia Dalfollohttp://lattes.cnpq.br/5569596745457696Auler, Neiva Maria Frizonhttp://lattes.cnpq.br/4721165773734294http://lattes.cnpq.br/0019735842865170Nora, Gracieli Dalla5001000000094005003003003006090974d-4c73-4bec-8e07-8cf0a1e0c33e752ebd41-f218-4500-9918-ec7d2f689c7471eec381-139f-440f-b1f3-c2bc737962a762a89b21-cd0a-4192-94b5-aa6d227b017cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDALLA NORA, GRACIELI.pdfapplication/pdf6997166http://repositorio.ufsm.br/bitstream/1/5059/1/DALLA%20NORA%2c%20GRACIELI.pdf978d45ee529579ff73b507d43772ce8fMD51TEXTDALLA NORA, GRACIELI.pdf.txtDALLA NORA, GRACIELI.pdf.txtExtracted texttext/plain55498http://repositorio.ufsm.br/bitstream/1/5059/2/DALLA%20NORA%2c%20GRACIELI.pdf.txt3aad34a94e8f3d5efb6daf8399996e85MD52THUMBNAILDALLA NORA, GRACIELI.pdf.jpgDALLA NORA, GRACIELI.pdf.jpgIM Thumbnailimage/jpeg4501http://repositorio.ufsm.br/bitstream/1/5059/3/DALLA%20NORA%2c%20GRACIELI.pdf.jpg9b14da45d5d2abe6d4cd5dd681e6ec6dMD531/50592022-03-15 08:36:44.954oai:repositorio.ufsm.br:1/5059Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132022-03-15T11:36:44Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Caracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.) |
dc.title.alternative.eng.fl_str_mv |
Cytogenetic characterization of common beans (Phaseolus vulgaris L.) |
title |
Caracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.) |
spellingShingle |
Caracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.) Nora, Gracieli Dalla Citogenética Phaseolus vulgaris L. Viabilidade polínica Cytogenetic Phaseolus vulgaris L. Pollen viability CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
title_short |
Caracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.) |
title_full |
Caracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.) |
title_fullStr |
Caracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.) |
title_full_unstemmed |
Caracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.) |
title_sort |
Caracterização citogenética de cultivares de feijão (Phaseolus vulgaris L.) |
author |
Nora, Gracieli Dalla |
author_facet |
Nora, Gracieli Dalla |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Tedesco, Solange Bosio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2541303074261787 |
dc.contributor.referee1.fl_str_mv |
Ribeiro, Nerinéia Dalfollo |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/5569596745457696 |
dc.contributor.referee2.fl_str_mv |
Auler, Neiva Maria Frizon |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4721165773734294 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0019735842865170 |
dc.contributor.author.fl_str_mv |
Nora, Gracieli Dalla |
contributor_str_mv |
Tedesco, Solange Bosio Ribeiro, Nerinéia Dalfollo Auler, Neiva Maria Frizon |
dc.subject.por.fl_str_mv |
Citogenética Phaseolus vulgaris L. Viabilidade polínica |
topic |
Citogenética Phaseolus vulgaris L. Viabilidade polínica Cytogenetic Phaseolus vulgaris L. Pollen viability CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
dc.subject.eng.fl_str_mv |
Cytogenetic Phaseolus vulgaris L. Pollen viability |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA |
description |
The meiotic behavior and estimation of pollen viability were studied in seven cultivars of common bean (Phaseolus vulgaris L.), which were IAPAR 44, Guapo Brilhante, BRS Expedito, BRS Valente, Guateian 6662, Pérola e Iraí. Floral buds were collected from cultivars, fixed and stored under refrigeration for the preparation of slides by squashing technique anthers and coloring with acetic orceína 2% and reactive Alexander. It was tried to obtain the greatest possible number of cells of each stage of meiosis. The estimation of pollen viability was observed by counting 800 pollen grains per plant. To verify changes in size, were measured 100 grains of pollen of each cultivar considered mature. There were no cells with abnormalities during the analysis of meiotic behavior in all cultivars. The meiotic index were high and uniform over 90%, indicating that these cultivars have the meiotic process stable and use in plant breeding not present problems. The pollen grains showed no significant differences as to its size, being similar among cultivars. Data on meiotic behavior and pollen viability obtained in this work combined with the literature, emphasize that the bean has a meiotic process uniform, showing high potential fertility of male gametes, which facilitates the use of this species in breeding programs. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-17 |
dc.date.accessioned.fl_str_mv |
2017-05-08 |
dc.date.available.fl_str_mv |
2017-05-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NORA, Gracieli Dalla. Cytogenetic characterization of common beans (Phaseolus vulgaris L.). 2012. 38 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5059 |
identifier_str_mv |
NORA, Gracieli Dalla. Cytogenetic characterization of common beans (Phaseolus vulgaris L.). 2012. 38 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
url |
http://repositorio.ufsm.br/handle/1/5059 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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500100000009 |
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400 500 300 300 300 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Agronomia |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Agronomia |
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Universidade Federal de Santa Maria |
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