Impacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrina
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/21906 |
Resumo: | Most changes in laboratory results, despite all the care taken to eliminate or minimize them, occur due to processes related to the pre-analytical phase. This stage may suffer interference, such as: time and temperature of storage of the samples for later analysis, submission of the samples to freeze/thaw cycles, among others, which may result in a reduction of the stability of the sample to be analyzed. As there are no short-term stability studies exploring iron status, this study aims to investigate the effects of freezing and thawing cycles on serum iron, transferrin and ferritin, as well as the impact of short-term storage on stability of these analytes. Serum samples from ten volunteers were subjected to three consecutive freezing cycles at -20°C and -80°C and thawing at 37°C on the same day. Serum aliquots were also kept at 4°C, -20°C and -80°C for twenty-eight days. Iron, ferritin and transferrin were measured after one, seven, fourteen, twenty-one or twenty-eight days of storage. Serum ferritin and transferrin varied from -2.6 to 2.6% and -1.7 to 2.4%, respectively, in their concentrations during the three freezing and thawing cycles at -20°C and -80°C, however, the variations were statistically significant only at -20°C (P=0.003 and P=0.002, respectively). No significant changes were found for iron at both temperatures. Storage at 4°C, -20°C and -80°C for up to four weeks significantly affected serum iron, ferritin and transferrin concentrations (P<0.01 for all). Due to the fact that iron, ferritin and transferrin were affected by the storage of samples at temperatures of 4°C, -20°C and -80°C for up to four weeks, and the freezing and thawing cycles at -20°C influenced the measurement of serum ferritin and transferrin, it is recommended, whenever possible, that iron status analytes be determined in fresh samples. |
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2021-08-12T11:32:40Z2021-08-12T11:32:40Z2020-08-07http://repositorio.ufsm.br/handle/1/21906Most changes in laboratory results, despite all the care taken to eliminate or minimize them, occur due to processes related to the pre-analytical phase. This stage may suffer interference, such as: time and temperature of storage of the samples for later analysis, submission of the samples to freeze/thaw cycles, among others, which may result in a reduction of the stability of the sample to be analyzed. As there are no short-term stability studies exploring iron status, this study aims to investigate the effects of freezing and thawing cycles on serum iron, transferrin and ferritin, as well as the impact of short-term storage on stability of these analytes. Serum samples from ten volunteers were subjected to three consecutive freezing cycles at -20°C and -80°C and thawing at 37°C on the same day. Serum aliquots were also kept at 4°C, -20°C and -80°C for twenty-eight days. Iron, ferritin and transferrin were measured after one, seven, fourteen, twenty-one or twenty-eight days of storage. Serum ferritin and transferrin varied from -2.6 to 2.6% and -1.7 to 2.4%, respectively, in their concentrations during the three freezing and thawing cycles at -20°C and -80°C, however, the variations were statistically significant only at -20°C (P=0.003 and P=0.002, respectively). No significant changes were found for iron at both temperatures. Storage at 4°C, -20°C and -80°C for up to four weeks significantly affected serum iron, ferritin and transferrin concentrations (P<0.01 for all). Due to the fact that iron, ferritin and transferrin were affected by the storage of samples at temperatures of 4°C, -20°C and -80°C for up to four weeks, and the freezing and thawing cycles at -20°C influenced the measurement of serum ferritin and transferrin, it is recommended, whenever possible, that iron status analytes be determined in fresh samples.A maioria das alterações nos resultados laboratoriais, apesar de todos os cuidados tomados para eliminá-las ou minimizá-las, ocorre devido a processos relacionados à fase pré-analítica. Essa etapa pode sofrer interferências, tais como: tempo e temperatura de armazenamento das amostras para posterior análise, submissão das amostras a ciclos de congelamento/descongelamento, entre outros, que poderão acarretar em uma redução da estabilidade da amostra a ser analisada. Por ainda não existirem estudos de estabilidade a curto prazo explorando o status do ferro, este estudo tem por objetivo investigar os efeitos de ciclos de congelamento/descongelamento sobre o ferro sérico, transferrina e ferritina, bem como o impacto do armazenamento a curto prazo na estabilidade desses analitos. Amostras de soro de dez voluntários foram submetidas a três ciclos consecutivos de congelamento a -20°C e -80°C e descongelamento a 37°C em um mesmo dia. Alíquotas de soro também foram mantidas a 4°C, -20°C e -80°C por vinte e oito dias. Ferro, ferritina e transferrina foram medidos após um, sete, quatorze, vinte e um ou vinte e oito dias de armazenamento. A ferritina e a transferrina séricas apresentaram variações de -2,6 a 2,6% e -1,7 a 2,4%, respectivamente, em suas concentrações durante os três ciclos de congelamento/descongelamento a -20°C e -80°C, no entanto, as variações foram estatisticamente significantes apenas a -20°C (P=0,003 e P=0,002, respectivamente). Não foram encontradas alterações significativas para o ferro nas duas temperaturas. O armazenamento nas temperaturas de 4°C, -20°C e - 80°C por até quatro semanas afetou significativamente as concentrações séricas de ferro, ferritina e transferrina (P<0,01 para todos). Devido ao fato de que ferro, ferritina e transferrina foram afetados pelo armazenamento de amostras a temperaturas de 4°C, -20°C e -80°C por até quatro semanas, e os ciclos de congelamento/descongelamento a -20°C influenciaram a medição da ferritina e transferrina no soro, recomenda-se, sempre que possível, que os analitos do status do ferro sejam determinados em amostras frescas.porUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBrasilAnálises Clínicas e ToxicológicasAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessEstabilidadeCiclo congelamento/descongelamentoFerroFerritinaTransferrinaStabilityFreeze/thaw cycleIronFerritinTransferrinCNPQ::CIENCIAS DA SAUDE::FARMACIAImpacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrinaImpact of short-term storage and multiple freezing / defrosting cycles on serum iron, ferritine and transferrin concentrationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMoresco, Rafael Noalhttp://lattes.cnpq.br/2269922709577261Paniz, ClóvisCichota, Luiz Carloshttp://lattes.cnpq.br/9264823770631590Klat, Rogério Luciano4003000000056006006006006007a69f2ef-75ae-4e01-b1d1-8295e8c94f97dc1da0d3-3cc4-4a49-b048-040b9b8a6005e698f80b-435f-4ff3-a9b9-bfbd65d0d21d95933922-ceda-470a-92d5-bc88d9dc0af9reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGCF_2020_KLAT_ROGERIO.pdfDIS_PPGCF_2020_KLAT_ROGERIO.pdfDissertaçãoapplication/pdf3053241http://repositorio.ufsm.br/bitstream/1/21906/1/DIS_PPGCF_2020_KLAT_ROGERIO.pdf10190ef4b80a884bf112f7632dff8268MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Impacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrina |
dc.title.alternative.eng.fl_str_mv |
Impact of short-term storage and multiple freezing / defrosting cycles on serum iron, ferritine and transferrin concentrations |
title |
Impacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrina |
spellingShingle |
Impacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrina Klat, Rogério Luciano Estabilidade Ciclo congelamento/descongelamento Ferro Ferritina Transferrina Stability Freeze/thaw cycle Iron Ferritin Transferrin CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Impacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrina |
title_full |
Impacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrina |
title_fullStr |
Impacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrina |
title_full_unstemmed |
Impacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrina |
title_sort |
Impacto do armazenamento a curto prazo e de múltiplos ciclos de congelamento/descongelamento sobre as concentrações séricas de ferro, ferritina e transferrina |
author |
Klat, Rogério Luciano |
author_facet |
Klat, Rogério Luciano |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Moresco, Rafael Noal |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2269922709577261 |
dc.contributor.referee1.fl_str_mv |
Paniz, Clóvis |
dc.contributor.referee2.fl_str_mv |
Cichota, Luiz Carlos |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9264823770631590 |
dc.contributor.author.fl_str_mv |
Klat, Rogério Luciano |
contributor_str_mv |
Moresco, Rafael Noal Paniz, Clóvis Cichota, Luiz Carlos |
dc.subject.por.fl_str_mv |
Estabilidade Ciclo congelamento/descongelamento Ferro Ferritina Transferrina |
topic |
Estabilidade Ciclo congelamento/descongelamento Ferro Ferritina Transferrina Stability Freeze/thaw cycle Iron Ferritin Transferrin CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Stability Freeze/thaw cycle Iron Ferritin Transferrin |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Most changes in laboratory results, despite all the care taken to eliminate or minimize them, occur due to processes related to the pre-analytical phase. This stage may suffer interference, such as: time and temperature of storage of the samples for later analysis, submission of the samples to freeze/thaw cycles, among others, which may result in a reduction of the stability of the sample to be analyzed. As there are no short-term stability studies exploring iron status, this study aims to investigate the effects of freezing and thawing cycles on serum iron, transferrin and ferritin, as well as the impact of short-term storage on stability of these analytes. Serum samples from ten volunteers were subjected to three consecutive freezing cycles at -20°C and -80°C and thawing at 37°C on the same day. Serum aliquots were also kept at 4°C, -20°C and -80°C for twenty-eight days. Iron, ferritin and transferrin were measured after one, seven, fourteen, twenty-one or twenty-eight days of storage. Serum ferritin and transferrin varied from -2.6 to 2.6% and -1.7 to 2.4%, respectively, in their concentrations during the three freezing and thawing cycles at -20°C and -80°C, however, the variations were statistically significant only at -20°C (P=0.003 and P=0.002, respectively). No significant changes were found for iron at both temperatures. Storage at 4°C, -20°C and -80°C for up to four weeks significantly affected serum iron, ferritin and transferrin concentrations (P<0.01 for all). Due to the fact that iron, ferritin and transferrin were affected by the storage of samples at temperatures of 4°C, -20°C and -80°C for up to four weeks, and the freezing and thawing cycles at -20°C influenced the measurement of serum ferritin and transferrin, it is recommended, whenever possible, that iron status analytes be determined in fresh samples. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-08-07 |
dc.date.accessioned.fl_str_mv |
2021-08-12T11:32:40Z |
dc.date.available.fl_str_mv |
2021-08-12T11:32:40Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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http://repositorio.ufsm.br/handle/1/21906 |
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por |
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400300000005 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Análises Clínicas e Toxicológicas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
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