Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/13765 |
Resumo: | The objective of this study was to evaluate the efficacy of 20% methylglycinediaacetic acid solution in the removal of the intracanal smear layer, using a scanning electron microscope (SEM), longitudinally and quantitatively. The chemical-mechanical preparation of the root canals of human upper central incisors was performed with the ProTaper system up to the instrument F5 and 2,5% NaOCl as an irrigating solution. The teeth were then cleaved and the dentin surface analyzed in the SEM under low vacuum. Images were obtained with 1000x magnifications of the cervical, middle and apical thirds of areas previously determined and completely covered by smear layer. The teeth were reassembled and divided into two groups that received the different solutions for final cleaning of the root canal for 3 minutes: G1 - MGDA and G2 - EDTA. After the final irrigation, the tooth halves were again separated and prepared for analysis of the dentin surface in the SEM in high vacuum. Images with the same magnifications were obtained from the same areas previously evaluated and, through Image J software, the percentage of the area of the open dentinal tubules was calculated in relation to the total area ofthe image. Test t was used for comparison between groups and ANOVA between the thirds of the root canal. The level of significance was 5%. Data presented normal distribution (Shapiro-Wilk test). There was no statistically significant difference in the efficacy of the root smear layer removal between the solutions and nor of their action between the thirds. It is concluded that MGDA solution may be an alternative to the use of EDTA in Endodontics and its properties should be investigated. |
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Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacéticoIn vitro evaluation of methylglycinediacetic acid on intracanal smear layer removalEDTAMicroscopia eletrônica de varreduraLama dentináriaIrrigantes do canal radicularEDTAScanning electron microscopySmear layerRoot canal irrigantsCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAThe objective of this study was to evaluate the efficacy of 20% methylglycinediaacetic acid solution in the removal of the intracanal smear layer, using a scanning electron microscope (SEM), longitudinally and quantitatively. The chemical-mechanical preparation of the root canals of human upper central incisors was performed with the ProTaper system up to the instrument F5 and 2,5% NaOCl as an irrigating solution. The teeth were then cleaved and the dentin surface analyzed in the SEM under low vacuum. Images were obtained with 1000x magnifications of the cervical, middle and apical thirds of areas previously determined and completely covered by smear layer. The teeth were reassembled and divided into two groups that received the different solutions for final cleaning of the root canal for 3 minutes: G1 - MGDA and G2 - EDTA. After the final irrigation, the tooth halves were again separated and prepared for analysis of the dentin surface in the SEM in high vacuum. Images with the same magnifications were obtained from the same areas previously evaluated and, through Image J software, the percentage of the area of the open dentinal tubules was calculated in relation to the total area ofthe image. Test t was used for comparison between groups and ANOVA between the thirds of the root canal. The level of significance was 5%. Data presented normal distribution (Shapiro-Wilk test). There was no statistically significant difference in the efficacy of the root smear layer removal between the solutions and nor of their action between the thirds. It is concluded that MGDA solution may be an alternative to the use of EDTA in Endodontics and its properties should be investigated.O objetivo deste estudo foi avaliar, longitudinal e quantitativamente, por Microscopia Eletrônica de Varredura (MEV), a eficácia da solução de ácido metilglicinodiacético a 20% (MGDA) na remoção da smear layer intracanal. O preparo químico-mecânico dos canais radiculares de incisivos centrais superiores humanos foi realizado com o sistema ProTaper até o instrumento F5 e NaOCl a 2,5% como solução irrigadora. Em seguida, os dentes foram clivados e a superfície dentinária analisada em MEV em baixo vácuo. Foram obtidas imagens com aumentos de 1000x dos terços cervical, médio e apical de áreas previamente determinadas e totalmente recobertas por smear layer. Os dentes foram remontados e divididos em dois grupos que receberam as diferentes soluções para limpeza final do conduto radicular por 3 minutos: G1 – MGDA e G2 – EDTA a 17%. Depois da irrigação final, as metades dos dentes foram novamente separadas e preparadas para análise da superfície dentinária em MEV em alto vácuo. Obteve-se imagens com os mesmos aumentos das mesmas áreas anteriormente avaliadas e, por meio do software Image J, foi calculada a porcentagem da área dos túbulos dentinários abertos em relação à área total da imagem. Test t foi utilizado para comparação entre grupos e ANOVA entre os terços do canal radicular. O nível de significância foi de 5%. Os dados apresentaram distribuição normal (teste Shapiro-Wilk). Não houve diferença estatisticamente significante na eficácia da remoção smear layer radicular entre as soluções e nem da ação destas entre os terços. Conclui-se que solução de MGDA pode ser uma alternativa ao uso do EDTA em Endodontia e suas propriedades devem ser pesquisadas.Universidade Federal de Santa MariaBrasilOdontologiaUFSMPrograma de Pós-Graduação em Ciências OdontológicasCentro de Ciências da SaúdeSchmitz, Marcia da Silvahttp://lattes.cnpq.br/4239733753424639Dullius, Angela Isabel dos Santoshttp://lattes.cnpq.br/9045765842523860Grecca, Fabiana Soareshttp://lattes.cnpq.br/1571244923940078Dotto, Ronise Ferreirahttp://lattes.cnpq.br/2163810633188295Montagner, Franciscohttp://lattes.cnpq.br/2937031469516770Dotto, Sidney Ricardohttp://lattes.cnpq.br/6534214233447363Morari, Víctor Hugo Carvalho2018-07-12T18:03:35Z2018-07-12T18:03:35Z2017-07-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/13765porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2018-07-12T18:03:36Zoai:repositorio.ufsm.br:1/13765Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2018-07-12T18:03:36Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.none.fl_str_mv |
Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético In vitro evaluation of methylglycinediacetic acid on intracanal smear layer removal |
title |
Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético |
spellingShingle |
Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético Morari, Víctor Hugo Carvalho EDTA Microscopia eletrônica de varredura Lama dentinária Irrigantes do canal radicular EDTA Scanning electron microscopy Smear layer Root canal irrigants CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético |
title_full |
Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético |
title_fullStr |
Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético |
title_full_unstemmed |
Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético |
title_sort |
Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético |
author |
Morari, Víctor Hugo Carvalho |
author_facet |
Morari, Víctor Hugo Carvalho |
author_role |
author |
dc.contributor.none.fl_str_mv |
Schmitz, Marcia da Silva http://lattes.cnpq.br/4239733753424639 Dullius, Angela Isabel dos Santos http://lattes.cnpq.br/9045765842523860 Grecca, Fabiana Soares http://lattes.cnpq.br/1571244923940078 Dotto, Ronise Ferreira http://lattes.cnpq.br/2163810633188295 Montagner, Francisco http://lattes.cnpq.br/2937031469516770 Dotto, Sidney Ricardo http://lattes.cnpq.br/6534214233447363 |
dc.contributor.author.fl_str_mv |
Morari, Víctor Hugo Carvalho |
dc.subject.por.fl_str_mv |
EDTA Microscopia eletrônica de varredura Lama dentinária Irrigantes do canal radicular EDTA Scanning electron microscopy Smear layer Root canal irrigants CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
topic |
EDTA Microscopia eletrônica de varredura Lama dentinária Irrigantes do canal radicular EDTA Scanning electron microscopy Smear layer Root canal irrigants CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The objective of this study was to evaluate the efficacy of 20% methylglycinediaacetic acid solution in the removal of the intracanal smear layer, using a scanning electron microscope (SEM), longitudinally and quantitatively. The chemical-mechanical preparation of the root canals of human upper central incisors was performed with the ProTaper system up to the instrument F5 and 2,5% NaOCl as an irrigating solution. The teeth were then cleaved and the dentin surface analyzed in the SEM under low vacuum. Images were obtained with 1000x magnifications of the cervical, middle and apical thirds of areas previously determined and completely covered by smear layer. The teeth were reassembled and divided into two groups that received the different solutions for final cleaning of the root canal for 3 minutes: G1 - MGDA and G2 - EDTA. After the final irrigation, the tooth halves were again separated and prepared for analysis of the dentin surface in the SEM in high vacuum. Images with the same magnifications were obtained from the same areas previously evaluated and, through Image J software, the percentage of the area of the open dentinal tubules was calculated in relation to the total area ofthe image. Test t was used for comparison between groups and ANOVA between the thirds of the root canal. The level of significance was 5%. Data presented normal distribution (Shapiro-Wilk test). There was no statistically significant difference in the efficacy of the root smear layer removal between the solutions and nor of their action between the thirds. It is concluded that MGDA solution may be an alternative to the use of EDTA in Endodontics and its properties should be investigated. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-28 2018-07-12T18:03:35Z 2018-07-12T18:03:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/13765 |
url |
http://repositorio.ufsm.br/handle/1/13765 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Odontologia UFSM Programa de Pós-Graduação em Ciências Odontológicas Centro de Ciências da Saúde |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Brasil Odontologia UFSM Programa de Pós-Graduação em Ciências Odontológicas Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1805922129829429248 |