Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético

Detalhes bibliográficos
Autor(a) principal: Morari, Víctor Hugo Carvalho
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: por
Título da fonte: Manancial - Repositório Digital da UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/13765
Resumo: The objective of this study was to evaluate the efficacy of 20% methylglycinediaacetic acid solution in the removal of the intracanal smear layer, using a scanning electron microscope (SEM), longitudinally and quantitatively. The chemical-mechanical preparation of the root canals of human upper central incisors was performed with the ProTaper system up to the instrument F5 and 2,5% NaOCl as an irrigating solution. The teeth were then cleaved and the dentin surface analyzed in the SEM under low vacuum. Images were obtained with 1000x magnifications of the cervical, middle and apical thirds of areas previously determined and completely covered by smear layer. The teeth were reassembled and divided into two groups that received the different solutions for final cleaning of the root canal for 3 minutes: G1 - MGDA and G2 - EDTA. After the final irrigation, the tooth halves were again separated and prepared for analysis of the dentin surface in the SEM in high vacuum. Images with the same magnifications were obtained from the same areas previously evaluated and, through Image J software, the percentage of the area of the open dentinal tubules was calculated in relation to the total area ofthe image. Test t was used for comparison between groups and ANOVA between the thirds of the root canal. The level of significance was 5%. Data presented normal distribution (Shapiro-Wilk test). There was no statistically significant difference in the efficacy of the root smear layer removal between the solutions and nor of their action between the thirds. It is concluded that MGDA solution may be an alternative to the use of EDTA in Endodontics and its properties should be investigated.
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spelling Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacéticoIn vitro evaluation of methylglycinediacetic acid on intracanal smear layer removalEDTAMicroscopia eletrônica de varreduraLama dentináriaIrrigantes do canal radicularEDTAScanning electron microscopySmear layerRoot canal irrigantsCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAThe objective of this study was to evaluate the efficacy of 20% methylglycinediaacetic acid solution in the removal of the intracanal smear layer, using a scanning electron microscope (SEM), longitudinally and quantitatively. The chemical-mechanical preparation of the root canals of human upper central incisors was performed with the ProTaper system up to the instrument F5 and 2,5% NaOCl as an irrigating solution. The teeth were then cleaved and the dentin surface analyzed in the SEM under low vacuum. Images were obtained with 1000x magnifications of the cervical, middle and apical thirds of areas previously determined and completely covered by smear layer. The teeth were reassembled and divided into two groups that received the different solutions for final cleaning of the root canal for 3 minutes: G1 - MGDA and G2 - EDTA. After the final irrigation, the tooth halves were again separated and prepared for analysis of the dentin surface in the SEM in high vacuum. Images with the same magnifications were obtained from the same areas previously evaluated and, through Image J software, the percentage of the area of the open dentinal tubules was calculated in relation to the total area ofthe image. Test t was used for comparison between groups and ANOVA between the thirds of the root canal. The level of significance was 5%. Data presented normal distribution (Shapiro-Wilk test). There was no statistically significant difference in the efficacy of the root smear layer removal between the solutions and nor of their action between the thirds. It is concluded that MGDA solution may be an alternative to the use of EDTA in Endodontics and its properties should be investigated.O objetivo deste estudo foi avaliar, longitudinal e quantitativamente, por Microscopia Eletrônica de Varredura (MEV), a eficácia da solução de ácido metilglicinodiacético a 20% (MGDA) na remoção da smear layer intracanal. O preparo químico-mecânico dos canais radiculares de incisivos centrais superiores humanos foi realizado com o sistema ProTaper até o instrumento F5 e NaOCl a 2,5% como solução irrigadora. Em seguida, os dentes foram clivados e a superfície dentinária analisada em MEV em baixo vácuo. Foram obtidas imagens com aumentos de 1000x dos terços cervical, médio e apical de áreas previamente determinadas e totalmente recobertas por smear layer. Os dentes foram remontados e divididos em dois grupos que receberam as diferentes soluções para limpeza final do conduto radicular por 3 minutos: G1 – MGDA e G2 – EDTA a 17%. Depois da irrigação final, as metades dos dentes foram novamente separadas e preparadas para análise da superfície dentinária em MEV em alto vácuo. Obteve-se imagens com os mesmos aumentos das mesmas áreas anteriormente avaliadas e, por meio do software Image J, foi calculada a porcentagem da área dos túbulos dentinários abertos em relação à área total da imagem. Test t foi utilizado para comparação entre grupos e ANOVA entre os terços do canal radicular. O nível de significância foi de 5%. Os dados apresentaram distribuição normal (teste Shapiro-Wilk). Não houve diferença estatisticamente significante na eficácia da remoção smear layer radicular entre as soluções e nem da ação destas entre os terços. Conclui-se que solução de MGDA pode ser uma alternativa ao uso do EDTA em Endodontia e suas propriedades devem ser pesquisadas.Universidade Federal de Santa MariaBrasilOdontologiaUFSMPrograma de Pós-Graduação em Ciências OdontológicasCentro de Ciências da SaúdeSchmitz, Marcia da Silvahttp://lattes.cnpq.br/4239733753424639Dullius, Angela Isabel dos Santoshttp://lattes.cnpq.br/9045765842523860Grecca, Fabiana Soareshttp://lattes.cnpq.br/1571244923940078Dotto, Ronise Ferreirahttp://lattes.cnpq.br/2163810633188295Montagner, Franciscohttp://lattes.cnpq.br/2937031469516770Dotto, Sidney Ricardohttp://lattes.cnpq.br/6534214233447363Morari, Víctor Hugo Carvalho2018-07-12T18:03:35Z2018-07-12T18:03:35Z2017-07-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://repositorio.ufsm.br/handle/1/13765porAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM2018-07-12T18:03:36Zoai:repositorio.ufsm.br:1/13765Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2018-07-12T18:03:36Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
In vitro evaluation of methylglycinediacetic acid on intracanal smear layer removal
title Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
spellingShingle Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
Morari, Víctor Hugo Carvalho
EDTA
Microscopia eletrônica de varredura
Lama dentinária
Irrigantes do canal radicular
EDTA
Scanning electron microscopy
Smear layer
Root canal irrigants
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
title_full Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
title_fullStr Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
title_full_unstemmed Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
title_sort Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
author Morari, Víctor Hugo Carvalho
author_facet Morari, Víctor Hugo Carvalho
author_role author
dc.contributor.none.fl_str_mv Schmitz, Marcia da Silva
http://lattes.cnpq.br/4239733753424639
Dullius, Angela Isabel dos Santos
http://lattes.cnpq.br/9045765842523860
Grecca, Fabiana Soares
http://lattes.cnpq.br/1571244923940078
Dotto, Ronise Ferreira
http://lattes.cnpq.br/2163810633188295
Montagner, Francisco
http://lattes.cnpq.br/2937031469516770
Dotto, Sidney Ricardo
http://lattes.cnpq.br/6534214233447363
dc.contributor.author.fl_str_mv Morari, Víctor Hugo Carvalho
dc.subject.por.fl_str_mv EDTA
Microscopia eletrônica de varredura
Lama dentinária
Irrigantes do canal radicular
EDTA
Scanning electron microscopy
Smear layer
Root canal irrigants
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
topic EDTA
Microscopia eletrônica de varredura
Lama dentinária
Irrigantes do canal radicular
EDTA
Scanning electron microscopy
Smear layer
Root canal irrigants
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
description The objective of this study was to evaluate the efficacy of 20% methylglycinediaacetic acid solution in the removal of the intracanal smear layer, using a scanning electron microscope (SEM), longitudinally and quantitatively. The chemical-mechanical preparation of the root canals of human upper central incisors was performed with the ProTaper system up to the instrument F5 and 2,5% NaOCl as an irrigating solution. The teeth were then cleaved and the dentin surface analyzed in the SEM under low vacuum. Images were obtained with 1000x magnifications of the cervical, middle and apical thirds of areas previously determined and completely covered by smear layer. The teeth were reassembled and divided into two groups that received the different solutions for final cleaning of the root canal for 3 minutes: G1 - MGDA and G2 - EDTA. After the final irrigation, the tooth halves were again separated and prepared for analysis of the dentin surface in the SEM in high vacuum. Images with the same magnifications were obtained from the same areas previously evaluated and, through Image J software, the percentage of the area of the open dentinal tubules was calculated in relation to the total area ofthe image. Test t was used for comparison between groups and ANOVA between the thirds of the root canal. The level of significance was 5%. Data presented normal distribution (Shapiro-Wilk test). There was no statistically significant difference in the efficacy of the root smear layer removal between the solutions and nor of their action between the thirds. It is concluded that MGDA solution may be an alternative to the use of EDTA in Endodontics and its properties should be investigated.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-28
2018-07-12T18:03:35Z
2018-07-12T18:03:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/13765
url http://repositorio.ufsm.br/handle/1/13765
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Odontologia
UFSM
Programa de Pós-Graduação em Ciências Odontológicas
Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Brasil
Odontologia
UFSM
Programa de Pós-Graduação em Ciências Odontológicas
Centro de Ciências da Saúde
dc.source.none.fl_str_mv reponame:Manancial - Repositório Digital da UFSM
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Manancial - Repositório Digital da UFSM
collection Manancial - Repositório Digital da UFSM
repository.name.fl_str_mv Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv atendimento.sib@ufsm.br||tedebc@gmail.com
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