Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético

Detalhes bibliográficos
Autor(a) principal: Morari, Víctor Hugo Carvalho
Data de Publicação: 2017
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UFSM
Texto Completo: http://repositorio.ufsm.br/handle/1/13765
Resumo: The objective of this study was to evaluate the efficacy of 20% methylglycinediaacetic acid solution in the removal of the intracanal smear layer, using a scanning electron microscope (SEM), longitudinally and quantitatively. The chemical-mechanical preparation of the root canals of human upper central incisors was performed with the ProTaper system up to the instrument F5 and 2,5% NaOCl as an irrigating solution. The teeth were then cleaved and the dentin surface analyzed in the SEM under low vacuum. Images were obtained with 1000x magnifications of the cervical, middle and apical thirds of areas previously determined and completely covered by smear layer. The teeth were reassembled and divided into two groups that received the different solutions for final cleaning of the root canal for 3 minutes: G1 - MGDA and G2 - EDTA. After the final irrigation, the tooth halves were again separated and prepared for analysis of the dentin surface in the SEM in high vacuum. Images with the same magnifications were obtained from the same areas previously evaluated and, through Image J software, the percentage of the area of the open dentinal tubules was calculated in relation to the total area ofthe image. Test t was used for comparison between groups and ANOVA between the thirds of the root canal. The level of significance was 5%. Data presented normal distribution (Shapiro-Wilk test). There was no statistically significant difference in the efficacy of the root smear layer removal between the solutions and nor of their action between the thirds. It is concluded that MGDA solution may be an alternative to the use of EDTA in Endodontics and its properties should be investigated.
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spelling 2018-07-12T18:03:35Z2018-07-12T18:03:35Z2017-07-28http://repositorio.ufsm.br/handle/1/13765The objective of this study was to evaluate the efficacy of 20% methylglycinediaacetic acid solution in the removal of the intracanal smear layer, using a scanning electron microscope (SEM), longitudinally and quantitatively. The chemical-mechanical preparation of the root canals of human upper central incisors was performed with the ProTaper system up to the instrument F5 and 2,5% NaOCl as an irrigating solution. The teeth were then cleaved and the dentin surface analyzed in the SEM under low vacuum. Images were obtained with 1000x magnifications of the cervical, middle and apical thirds of areas previously determined and completely covered by smear layer. The teeth were reassembled and divided into two groups that received the different solutions for final cleaning of the root canal for 3 minutes: G1 - MGDA and G2 - EDTA. After the final irrigation, the tooth halves were again separated and prepared for analysis of the dentin surface in the SEM in high vacuum. Images with the same magnifications were obtained from the same areas previously evaluated and, through Image J software, the percentage of the area of the open dentinal tubules was calculated in relation to the total area ofthe image. Test t was used for comparison between groups and ANOVA between the thirds of the root canal. The level of significance was 5%. Data presented normal distribution (Shapiro-Wilk test). There was no statistically significant difference in the efficacy of the root smear layer removal between the solutions and nor of their action between the thirds. It is concluded that MGDA solution may be an alternative to the use of EDTA in Endodontics and its properties should be investigated.O objetivo deste estudo foi avaliar, longitudinal e quantitativamente, por Microscopia Eletrônica de Varredura (MEV), a eficácia da solução de ácido metilglicinodiacético a 20% (MGDA) na remoção da smear layer intracanal. O preparo químico-mecânico dos canais radiculares de incisivos centrais superiores humanos foi realizado com o sistema ProTaper até o instrumento F5 e NaOCl a 2,5% como solução irrigadora. Em seguida, os dentes foram clivados e a superfície dentinária analisada em MEV em baixo vácuo. Foram obtidas imagens com aumentos de 1000x dos terços cervical, médio e apical de áreas previamente determinadas e totalmente recobertas por smear layer. Os dentes foram remontados e divididos em dois grupos que receberam as diferentes soluções para limpeza final do conduto radicular por 3 minutos: G1 – MGDA e G2 – EDTA a 17%. Depois da irrigação final, as metades dos dentes foram novamente separadas e preparadas para análise da superfície dentinária em MEV em alto vácuo. Obteve-se imagens com os mesmos aumentos das mesmas áreas anteriormente avaliadas e, por meio do software Image J, foi calculada a porcentagem da área dos túbulos dentinários abertos em relação à área total da imagem. Test t foi utilizado para comparação entre grupos e ANOVA entre os terços do canal radicular. O nível de significância foi de 5%. Os dados apresentaram distribuição normal (teste Shapiro-Wilk). Não houve diferença estatisticamente significante na eficácia da remoção smear layer radicular entre as soluções e nem da ação destas entre os terços. Conclui-se que solução de MGDA pode ser uma alternativa ao uso do EDTA em Endodontia e suas propriedades devem ser pesquisadas.porUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em Ciências OdontológicasUFSMBrasilOdontologiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessEDTAMicroscopia eletrônica de varreduraLama dentináriaIrrigantes do canal radicularEDTAScanning electron microscopySmear layerRoot canal irrigantsCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAAvaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacéticoIn vitro evaluation of methylglycinediacetic acid on intracanal smear layer removalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisSchmitz, Marcia da Silvahttp://lattes.cnpq.br/4239733753424639Dullius, Angela Isabel dos Santoshttp://lattes.cnpq.br/9045765842523860Grecca, Fabiana Soareshttp://lattes.cnpq.br/1571244923940078Dotto, Ronise Ferreirahttp://lattes.cnpq.br/2163810633188295Montagner, Franciscohttp://lattes.cnpq.br/2937031469516770Dotto, Sidney Ricardohttp://lattes.cnpq.br/6534214233447363http://lattes.cnpq.br/7319922923291102Morari, Víctor Hugo Carvalho400200000000600fc4da2ba-b68a-44fb-b92f-a1a7893aad092d88980b-6e59-4ffa-8c9b-e4afbbd65f75edd4f8c0-fc7b-4169-980a-10d28961ade9333c7e8d-6b21-4746-b8fa-f144603c110e6c9e2592-69d2-4bf8-9ac0-15ee7f643ba7328f98cc-8bff-49bf-b862-d144db2f1f85b5578493-d1b4-4b66-8979-b9f4bddeb9c5reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
dc.title.alternative.eng.fl_str_mv In vitro evaluation of methylglycinediacetic acid on intracanal smear layer removal
title Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
spellingShingle Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
Morari, Víctor Hugo Carvalho
EDTA
Microscopia eletrônica de varredura
Lama dentinária
Irrigantes do canal radicular
EDTA
Scanning electron microscopy
Smear layer
Root canal irrigants
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
title_full Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
title_fullStr Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
title_full_unstemmed Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
title_sort Avaliação in vitro da remoção da smear laer intracanal pelo ácido metilglicinodiacético
author Morari, Víctor Hugo Carvalho
author_facet Morari, Víctor Hugo Carvalho
author_role author
dc.contributor.advisor1.fl_str_mv Schmitz, Marcia da Silva
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/4239733753424639
dc.contributor.advisor-co1.fl_str_mv Dullius, Angela Isabel dos Santos
dc.contributor.advisor-co1Lattes.fl_str_mv http://lattes.cnpq.br/9045765842523860
dc.contributor.referee1.fl_str_mv Grecca, Fabiana Soares
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/1571244923940078
dc.contributor.referee2.fl_str_mv Dotto, Ronise Ferreira
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/2163810633188295
dc.contributor.referee3.fl_str_mv Montagner, Francisco
dc.contributor.referee3Lattes.fl_str_mv http://lattes.cnpq.br/2937031469516770
dc.contributor.referee4.fl_str_mv Dotto, Sidney Ricardo
dc.contributor.referee4Lattes.fl_str_mv http://lattes.cnpq.br/6534214233447363
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7319922923291102
dc.contributor.author.fl_str_mv Morari, Víctor Hugo Carvalho
contributor_str_mv Schmitz, Marcia da Silva
Dullius, Angela Isabel dos Santos
Grecca, Fabiana Soares
Dotto, Ronise Ferreira
Montagner, Francisco
Dotto, Sidney Ricardo
dc.subject.por.fl_str_mv EDTA
Microscopia eletrônica de varredura
Lama dentinária
Irrigantes do canal radicular
topic EDTA
Microscopia eletrônica de varredura
Lama dentinária
Irrigantes do canal radicular
EDTA
Scanning electron microscopy
Smear layer
Root canal irrigants
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
dc.subject.eng.fl_str_mv EDTA
Scanning electron microscopy
Smear layer
Root canal irrigants
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
description The objective of this study was to evaluate the efficacy of 20% methylglycinediaacetic acid solution in the removal of the intracanal smear layer, using a scanning electron microscope (SEM), longitudinally and quantitatively. The chemical-mechanical preparation of the root canals of human upper central incisors was performed with the ProTaper system up to the instrument F5 and 2,5% NaOCl as an irrigating solution. The teeth were then cleaved and the dentin surface analyzed in the SEM under low vacuum. Images were obtained with 1000x magnifications of the cervical, middle and apical thirds of areas previously determined and completely covered by smear layer. The teeth were reassembled and divided into two groups that received the different solutions for final cleaning of the root canal for 3 minutes: G1 - MGDA and G2 - EDTA. After the final irrigation, the tooth halves were again separated and prepared for analysis of the dentin surface in the SEM in high vacuum. Images with the same magnifications were obtained from the same areas previously evaluated and, through Image J software, the percentage of the area of the open dentinal tubules was calculated in relation to the total area ofthe image. Test t was used for comparison between groups and ANOVA between the thirds of the root canal. The level of significance was 5%. Data presented normal distribution (Shapiro-Wilk test). There was no statistically significant difference in the efficacy of the root smear layer removal between the solutions and nor of their action between the thirds. It is concluded that MGDA solution may be an alternative to the use of EDTA in Endodontics and its properties should be investigated.
publishDate 2017
dc.date.issued.fl_str_mv 2017-07-28
dc.date.accessioned.fl_str_mv 2018-07-12T18:03:35Z
dc.date.available.fl_str_mv 2018-07-12T18:03:35Z
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http://creativecommons.org/licenses/by-nc-nd/4.0/
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dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências da Saúde
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Ciências Odontológicas
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Odontologia
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências da Saúde
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