Disseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongos
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/24354 |
Resumo: | m-Trifluoromethyl-diphenyldiselenide (m-CF3-PhSe)2 has multiple targets, including the glutamatergic system and the opioid, in addition to having antioxidant properties. Behavioral locomotor sensitization, characterized by hyperactivity, is a drug addiction and dependence model used experimentally. Several pathways contribute to these aspects, such as the opioid system, dopamine and glutamate receptors modulation. Furthermore, redox imbalance favors this condition. This study aimed to investigate the effect of (m-CF3-PhSe)2 on the acquisition, morphine withdrawal, and drug re-exposure phases on morphine-induced behavioral locomotor sensitization in mice. This study used 30 day-old male Swiss mice (CEUA 5302070619). They were treated with saline or morphine 10 mg/kg twice a day for three days; in the next five days, they were kept abstinent, and received saline or morphine on the ninth day. To assess the compound effect on this protocol, (m-CF3-PhSe)2 was administered during acquisition (first 3 days), on morphine withdrawal or re-exposure (ninth day), soon after the morphine dose on the ninth day, the mice were challenged in the locomotor activity test. Markers of oxidative stress were determined, in addition to protein levels of opioid, dopamine, and glutamate receptors in the mouse cerebral cortex. The results showed that re-exposure to morphine increased the content of opioid (MOR, DOR and KOR) and glutamatergic receptors (NMDA 2A and 2B). However, it decreased the levels of dopamine receptors (D1 and D2), in addition to triggering a redox imbalance, as it increased the levels of reactive species and markers of lipid peroxidation and altered the activity of antioxidant enzymes. In conclusion, (m-CF3-PhSe)2 attenuated morphine-induced locomotor sensitization and protected against biochemical and molecular alterations caused by re-exposure to morphine in mice. |
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2022-05-13T15:17:36Z2022-05-13T15:17:36Z2022-02-11http://repositorio.ufsm.br/handle/1/24354m-Trifluoromethyl-diphenyldiselenide (m-CF3-PhSe)2 has multiple targets, including the glutamatergic system and the opioid, in addition to having antioxidant properties. Behavioral locomotor sensitization, characterized by hyperactivity, is a drug addiction and dependence model used experimentally. Several pathways contribute to these aspects, such as the opioid system, dopamine and glutamate receptors modulation. Furthermore, redox imbalance favors this condition. This study aimed to investigate the effect of (m-CF3-PhSe)2 on the acquisition, morphine withdrawal, and drug re-exposure phases on morphine-induced behavioral locomotor sensitization in mice. This study used 30 day-old male Swiss mice (CEUA 5302070619). They were treated with saline or morphine 10 mg/kg twice a day for three days; in the next five days, they were kept abstinent, and received saline or morphine on the ninth day. To assess the compound effect on this protocol, (m-CF3-PhSe)2 was administered during acquisition (first 3 days), on morphine withdrawal or re-exposure (ninth day), soon after the morphine dose on the ninth day, the mice were challenged in the locomotor activity test. Markers of oxidative stress were determined, in addition to protein levels of opioid, dopamine, and glutamate receptors in the mouse cerebral cortex. The results showed that re-exposure to morphine increased the content of opioid (MOR, DOR and KOR) and glutamatergic receptors (NMDA 2A and 2B). However, it decreased the levels of dopamine receptors (D1 and D2), in addition to triggering a redox imbalance, as it increased the levels of reactive species and markers of lipid peroxidation and altered the activity of antioxidant enzymes. In conclusion, (m-CF3-PhSe)2 attenuated morphine-induced locomotor sensitization and protected against biochemical and molecular alterations caused by re-exposure to morphine in mice.O disseleneto de m-trifluormetil-difenila (m-CF3-PhSe)2 possui múltiplos alvos, incluindo o sistema glutamatérgico e o opioide, além de possuir propriedades antioxidantes. A sensibilização locomotora comportamental, caracterizada pela hiperatividade, é um modelo utilizado em estudos relacionados à drogadição e dependência, e diversas vias contribuem com estes aspectos, como o sistema opioide e a modulação de receptores de dopamina e de glutamato. Além do mais, o desequilíbrio redox favorece esta condição. O objetivo deste trabalho foi investigar o efeito do (m-CF3-PhSe)2 nas fases de aquisição, retirada da morfina e na reexposição à droga na sensibilização locomotora comportamental induzida por morfina em camundongos. Para este estudo foram utilizados camundongos Swiss machos com 30 dias (CEUA 5302070619), estes foram tratados com salina ou morfina 10 mg/kg 2x ao dia durante 3 dias, permaneceram em abstinência nos próximos 5 dias e no nono dia receberam uma dose de salina ou morfina. A fim de avaliar o efeito do composto, o (m-CF3-PhSe)2 foi administrado durante a aquisição (primeiros 3 dias), na retirada da morfina ou na reexposição (nono dia), logo após a dose de morfina no nono dia, imediatamente a atividade locomotora dos animais foi avaliada. Determinamos marcadores de estresse oxidativo, além dos níveis proteicos dos receptores opioides, de dopamina e de glutamato em córtex cerebral. Os resultados mostraram que a reexposição à morfina aumentou o conteúdo dos receptores opioides (MOR, DOR e KOR) e dos receptores glutamatérgicos (NMDA 2A e 2B). Entretanto, diminuiu os níveis dos receptores de dopamina (D1 e D2), além de desencadear um desequilíbrio redox, visto que aumentou os níveis de espécies reativas e marcadores da peroxidação lipídica e alterou a atividade de enzimas antioxidantes. Conclui-se que o (m-CF3-PhSe)2 foi efetivo em atenuar a sensibilização locomotora induzida por morfina, além de proteger contra as alterações bioquímicas e moleculares causadas pela reexposição a morfina em camundongos.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAtividade locomotoraDrogadiçãoReceptor dopaminérgicoReceptor glutamatérgicoOpioideEspécies reativasLocomotor activityDrug addictionDopaminergic receptorGlutamatergic receptorOpioidReactive speciesCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICADisseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongosm-Trifluormethyl-diphenyl diselenide attenuates all phases of morphine-induced behavioral locomotor sensitization in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisNogueira, Cristina Waynehttp://lattes.cnpq.br/2877042401245169Bruning, César AugustoOliveira, Sara Marchesan dehttp://lattes.cnpq.br/6095083687749431Rodrigues, Renata Fritzsche2008000000026006006006006000186436d-f662-4a5e-b36e-8c8ab8e10bf8f881935f-a8fe-4513-b867-7c904e006b641da0da6d-e049-4825-8f9e-43355a3430f34bf6b0f2-e678-4638-8254-7ffc6b173f0freponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Disseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongos |
dc.title.alternative.eng.fl_str_mv |
m-Trifluormethyl-diphenyl diselenide attenuates all phases of morphine-induced behavioral locomotor sensitization in mice |
title |
Disseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongos |
spellingShingle |
Disseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongos Rodrigues, Renata Fritzsche Atividade locomotora Drogadição Receptor dopaminérgico Receptor glutamatérgico Opioide Espécies reativas Locomotor activity Drug addiction Dopaminergic receptor Glutamatergic receptor Opioid Reactive species CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Disseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongos |
title_full |
Disseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongos |
title_fullStr |
Disseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongos |
title_full_unstemmed |
Disseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongos |
title_sort |
Disseleneto m-trifluormetil difenila atenua as fases da sensibilização motora comportamental induzidas por morfina em camundongos |
author |
Rodrigues, Renata Fritzsche |
author_facet |
Rodrigues, Renata Fritzsche |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Nogueira, Cristina Wayne |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2877042401245169 |
dc.contributor.referee1.fl_str_mv |
Bruning, César Augusto |
dc.contributor.referee2.fl_str_mv |
Oliveira, Sara Marchesan de |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6095083687749431 |
dc.contributor.author.fl_str_mv |
Rodrigues, Renata Fritzsche |
contributor_str_mv |
Nogueira, Cristina Wayne Bruning, César Augusto Oliveira, Sara Marchesan de |
dc.subject.por.fl_str_mv |
Atividade locomotora Drogadição Receptor dopaminérgico Receptor glutamatérgico Opioide Espécies reativas |
topic |
Atividade locomotora Drogadição Receptor dopaminérgico Receptor glutamatérgico Opioide Espécies reativas Locomotor activity Drug addiction Dopaminergic receptor Glutamatergic receptor Opioid Reactive species CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Locomotor activity Drug addiction Dopaminergic receptor Glutamatergic receptor Opioid Reactive species |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
m-Trifluoromethyl-diphenyldiselenide (m-CF3-PhSe)2 has multiple targets, including the glutamatergic system and the opioid, in addition to having antioxidant properties. Behavioral locomotor sensitization, characterized by hyperactivity, is a drug addiction and dependence model used experimentally. Several pathways contribute to these aspects, such as the opioid system, dopamine and glutamate receptors modulation. Furthermore, redox imbalance favors this condition. This study aimed to investigate the effect of (m-CF3-PhSe)2 on the acquisition, morphine withdrawal, and drug re-exposure phases on morphine-induced behavioral locomotor sensitization in mice. This study used 30 day-old male Swiss mice (CEUA 5302070619). They were treated with saline or morphine 10 mg/kg twice a day for three days; in the next five days, they were kept abstinent, and received saline or morphine on the ninth day. To assess the compound effect on this protocol, (m-CF3-PhSe)2 was administered during acquisition (first 3 days), on morphine withdrawal or re-exposure (ninth day), soon after the morphine dose on the ninth day, the mice were challenged in the locomotor activity test. Markers of oxidative stress were determined, in addition to protein levels of opioid, dopamine, and glutamate receptors in the mouse cerebral cortex. The results showed that re-exposure to morphine increased the content of opioid (MOR, DOR and KOR) and glutamatergic receptors (NMDA 2A and 2B). However, it decreased the levels of dopamine receptors (D1 and D2), in addition to triggering a redox imbalance, as it increased the levels of reactive species and markers of lipid peroxidation and altered the activity of antioxidant enzymes. In conclusion, (m-CF3-PhSe)2 attenuated morphine-induced locomotor sensitization and protected against biochemical and molecular alterations caused by re-exposure to morphine in mice. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-05-13T15:17:36Z |
dc.date.available.fl_str_mv |
2022-05-13T15:17:36Z |
dc.date.issued.fl_str_mv |
2022-02-11 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/24354 |
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http://repositorio.ufsm.br/handle/1/24354 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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200800000002 |
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600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
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UFSM |
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Brasil |
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Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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