Obtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativas
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional Manancial UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/23331 |
Resumo: | This work had as objective to obtain extracts of olive leaves (OLE) through the technique of extraction assisted by microwaves for later application of the extracts obtained in the elaboration of active biodegradable films. The extraction process, as well as the influence of the process parameters on the content of bioactive compounds present in the aqueous extract, were studied using design of experiments. The content of total phenolic compounds (PC), the antioxidant activity (AA), the antimicrobial activity of OLE against Escherichia coli (E. coli) and the content of oleuropein obtained under optimal conditions of extraction were the responses evaluated. In addition, methods of extraction by maceration and assisted by ultrasound to obtain olive leaf extracts were studied. Afterwards, carrageenan films were made with and without the addition of OLE by the casting method. The films were evaluated for color, solubility, thickness, water vapor permeability and mechanical properties. The protective effect of films against antimicrobial activity on lamb meat packaging was also evaluated. The optimal conditions for microwave assisted extraction, using the desirability statistical tool, were: 100°C, 2 min and pH 6. Under these conditions it is possible to obtain AA of 92.87%, PC of 103.87 mgGAE.g–¹ (d.b), in addition to antimicrobial activity against E. coli, with a minimum inhibitory concentration value of 50 mg.mL-¹ and oleuropein content of 11.59 mg.g-¹ (d.b). When compared to the extraction technologies by maceration and ultrasound, the microwave technology proved to be more efficient, presenting a greater recovery of bioactive compounds of interest. The incorporation of OLE in the carrageenan matrix caused changes in the properties of the films, with an increase in thickness, elongation and permeability to water vapor, and a decrease in the tensile strength and elasticity modulus of the film. The solubility was not significantly affected by the addition of OLE, however the color difference with the addition of OLE was 64.72%. Another important result was the in vivo analysis of psychrophiles, that is, when applied to the packaging of lamb meat, carrageenan films with extract had an inhibitory effect on the count of psychrophiles. The study revealed the benefits of olive leaf extract, obtained by microwave incorporated in films based on carrageenan with potential for application as active packaging. |
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2021-12-15T19:05:31Z2021-12-15T19:05:31Z2021-03-26http://repositorio.ufsm.br/handle/1/23331This work had as objective to obtain extracts of olive leaves (OLE) through the technique of extraction assisted by microwaves for later application of the extracts obtained in the elaboration of active biodegradable films. The extraction process, as well as the influence of the process parameters on the content of bioactive compounds present in the aqueous extract, were studied using design of experiments. The content of total phenolic compounds (PC), the antioxidant activity (AA), the antimicrobial activity of OLE against Escherichia coli (E. coli) and the content of oleuropein obtained under optimal conditions of extraction were the responses evaluated. In addition, methods of extraction by maceration and assisted by ultrasound to obtain olive leaf extracts were studied. Afterwards, carrageenan films were made with and without the addition of OLE by the casting method. The films were evaluated for color, solubility, thickness, water vapor permeability and mechanical properties. The protective effect of films against antimicrobial activity on lamb meat packaging was also evaluated. The optimal conditions for microwave assisted extraction, using the desirability statistical tool, were: 100°C, 2 min and pH 6. Under these conditions it is possible to obtain AA of 92.87%, PC of 103.87 mgGAE.g–¹ (d.b), in addition to antimicrobial activity against E. coli, with a minimum inhibitory concentration value of 50 mg.mL-¹ and oleuropein content of 11.59 mg.g-¹ (d.b). When compared to the extraction technologies by maceration and ultrasound, the microwave technology proved to be more efficient, presenting a greater recovery of bioactive compounds of interest. The incorporation of OLE in the carrageenan matrix caused changes in the properties of the films, with an increase in thickness, elongation and permeability to water vapor, and a decrease in the tensile strength and elasticity modulus of the film. The solubility was not significantly affected by the addition of OLE, however the color difference with the addition of OLE was 64.72%. Another important result was the in vivo analysis of psychrophiles, that is, when applied to the packaging of lamb meat, carrageenan films with extract had an inhibitory effect on the count of psychrophiles. The study revealed the benefits of olive leaf extract, obtained by microwave incorporated in films based on carrageenan with potential for application as active packaging.Este trabalho teve como objetivo obter extratos de folhas de oliveira (EFO) através da técnica de extração assistida por micro-ondas para posterior aplicação dos extratos obtidos na elaboração de filmes biodegradáveis ativos. O processo de extração, assim como a influência dos parâmetros do processo sobre o conteúdo de compostos bioativos presentes no extrato aquoso foram estudados utilizando planejamento de experimentos. O conteúdo de compostos fenólicos totais (FT), a atividade antioxidante (AA), a atividade antimicrobiana do EFO contra a Escherichia coli (E. coli) e o conteúdo de oleuropeína obtidos sob condições ótimas de extração foram às respostas avaliadas. Complementarmente, foram estudados os métodos de extração por maceração e assistido por ultrassom para obtenção dos extratos de folha de oliveira. Após, foram elaborados filmes à base de carragenana com e sem a adição de EFO pelo método de casting. Os filmes foram avaliados quanto à cor, solubilidade, espessura, permeabilidade ao vapor d’água e propriedades mecânicas. O efeito protetor dos filmes contra a atividade antimicrobiana na embalagem de carne de cordeiro também foi avaliado. As condições ótimas de extração assistida por micro-ondas, utilizando a ferramenta estatística da desejabilidade, foram: 100°C, 2 min e pH 6. Nessas condições é possível obter AA de 92,87%, FT de 103,87 mgGAE.g–¹ (b.s.), além de atividade antimicrobiana contra E. coli, com valor de concentração inibitória mínima de 50 mg.mL-¹ e conteúdo de oleuropeína de 11,59 mg.g-¹ (b.s.). Quando comparada às tecnologias de extração por maceração e ultrassom, a tecnologia de micro-ondas se mostrou mais eficiente, apresentando uma maior recuperação de compostos bioativos de interesse. A incorporação do EFO na matriz de carragenana provocou mudanças nas propriedades dos filmes, com o aumento na espessura, na elongação e na permeabilidade ao vapor d’água, e a diminuição na tensão de ruptura e no módulo de elasticidade do filme. A solubilidade não foi significativamente afetada pela adição do EFO, porém a diferença de cor com a adição de EFO foi de 64,72%. Outro importante resultado foi o da análise in vivo de psicrófilos, ou seja, quando aplicados na embalagem de carne de cordeiro os filmes de carragenana com extrato apresentaram efeito inibitório na contagem de psicrófilos. O estudo revelou os benefícios do extrato de folhas de oliveira, obtido por micro-ondas incorporado em filmes à base de carragenana com potencial para a aplicação como embalagens ativas.porUniversidade Federal de Santa MariaCentro de TecnologiaPrograma de Pós-Graduação em Engenharia QuímicaUFSMBrasilEngenharia QuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessOlea europeaCarragenanaMicro-ondasFilme biodegradávelCarrageenanMicrowaveBiodegradable filmCNPQ::ENGENHARIAS::ENGENHARIA QUIMICAObtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativasObtaining olive leaf extracts for application as an antimicrobial agent in active biodegradable packaginginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisDotto, Guilherme Luizhttp://lattes.cnpq.br/5412544199323879Rosa, Gabriela Silveira dahttp://lattes.cnpq.br/2813439231499190Moraes, Caroline Costa deMoraes, Mariana Agostini deKuhn, Raquel CristineKuhn, kátia Reginahttp://lattes.cnpq.br/9888865487251627Martiny, Thamiris Renata300600000006600600600600600600600600cb4c4429-649d-4998-a6b3-cfcf481ab6b9c0de45a2-9490-452b-a9a4-c3c153d65ff4f70143bc-bf2d-4edd-a8df-5b39853165a89edad6fc-0ecf-47f6-aca2-888fc57e55e858ed9bd4-4cb2-49a8-8317-ad35ca47a374a89ab1d6-91a7-4b55-a630-a56988550230aaccddc4-5cca-4ed8-b15a-72de6b0b9677reponame:Repositório Institucional Manancial UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALTES_PPGEQ_2021_MARTINY_THAMIRIS.pdfTES_PPGEQ_2021_MARTINY_THAMIRIS.pdfTese de doutoradoapplication/pdf3419673http://repositorio.ufsm.br/bitstream/1/23331/1/TES_PPGEQ_2021_MARTINY_THAMIRIS.pdf5bb39111f559a976a5f2bf30d56fc1baMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/23331/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-816http://repositorio.ufsm.br/bitstream/1/23331/3/license.txtf8fcb28efb1c8cf0dc096bec902bf4c4MD53TEXTTES_PPGEQ_2021_MARTINY_THAMIRIS.pdf.txtTES_PPGEQ_2021_MARTINY_THAMIRIS.pdf.txtExtracted texttext/plain268759http://repositorio.ufsm.br/bitstream/1/23331/4/TES_PPGEQ_2021_MARTINY_THAMIRIS.pdf.txt6f14df4d4025d2b75429d9d44290e5b6MD54THUMBNAILTES_PPGEQ_2021_MARTINY_THAMIRIS.pdf.jpgTES_PPGEQ_2021_MARTINY_THAMIRIS.pdf.jpgIM Thumbnailimage/jpeg4624http://repositorio.ufsm.br/bitstream/1/23331/5/TES_PPGEQ_2021_MARTINY_THAMIRIS.pdf.jpg46dc387a8d140e74838b33a6f986694dMD551/233312021-12-16 03:03:42.828oai:repositorio.ufsm.br:1/23331Q3JlYXRpdmUgQ29tbW9ucw==Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestouvidoria@ufsm.bropendoar:39132021-12-16T06:03:42Repositório Institucional Manancial UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Obtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativas |
dc.title.alternative.eng.fl_str_mv |
Obtaining olive leaf extracts for application as an antimicrobial agent in active biodegradable packaging |
title |
Obtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativas |
spellingShingle |
Obtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativas Martiny, Thamiris Renata Olea europea Carragenana Micro-ondas Filme biodegradável Carrageenan Microwave Biodegradable film CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Obtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativas |
title_full |
Obtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativas |
title_fullStr |
Obtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativas |
title_full_unstemmed |
Obtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativas |
title_sort |
Obtenção de extratos de folhas de oliveira para aplicação como agente antimicrobiano em embalagens biodegradáveis ativas |
author |
Martiny, Thamiris Renata |
author_facet |
Martiny, Thamiris Renata |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Dotto, Guilherme Luiz |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5412544199323879 |
dc.contributor.advisor-co1.fl_str_mv |
Rosa, Gabriela Silveira da |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/2813439231499190 |
dc.contributor.referee1.fl_str_mv |
Moraes, Caroline Costa de |
dc.contributor.referee2.fl_str_mv |
Moraes, Mariana Agostini de |
dc.contributor.referee3.fl_str_mv |
Kuhn, Raquel Cristine |
dc.contributor.referee4.fl_str_mv |
Kuhn, kátia Regina |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9888865487251627 |
dc.contributor.author.fl_str_mv |
Martiny, Thamiris Renata |
contributor_str_mv |
Dotto, Guilherme Luiz Rosa, Gabriela Silveira da Moraes, Caroline Costa de Moraes, Mariana Agostini de Kuhn, Raquel Cristine Kuhn, kátia Regina |
dc.subject.por.fl_str_mv |
Olea europea Carragenana Micro-ondas Filme biodegradável |
topic |
Olea europea Carragenana Micro-ondas Filme biodegradável Carrageenan Microwave Biodegradable film CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.eng.fl_str_mv |
Carrageenan Microwave Biodegradable film |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
description |
This work had as objective to obtain extracts of olive leaves (OLE) through the technique of extraction assisted by microwaves for later application of the extracts obtained in the elaboration of active biodegradable films. The extraction process, as well as the influence of the process parameters on the content of bioactive compounds present in the aqueous extract, were studied using design of experiments. The content of total phenolic compounds (PC), the antioxidant activity (AA), the antimicrobial activity of OLE against Escherichia coli (E. coli) and the content of oleuropein obtained under optimal conditions of extraction were the responses evaluated. In addition, methods of extraction by maceration and assisted by ultrasound to obtain olive leaf extracts were studied. Afterwards, carrageenan films were made with and without the addition of OLE by the casting method. The films were evaluated for color, solubility, thickness, water vapor permeability and mechanical properties. The protective effect of films against antimicrobial activity on lamb meat packaging was also evaluated. The optimal conditions for microwave assisted extraction, using the desirability statistical tool, were: 100°C, 2 min and pH 6. Under these conditions it is possible to obtain AA of 92.87%, PC of 103.87 mgGAE.g–¹ (d.b), in addition to antimicrobial activity against E. coli, with a minimum inhibitory concentration value of 50 mg.mL-¹ and oleuropein content of 11.59 mg.g-¹ (d.b). When compared to the extraction technologies by maceration and ultrasound, the microwave technology proved to be more efficient, presenting a greater recovery of bioactive compounds of interest. The incorporation of OLE in the carrageenan matrix caused changes in the properties of the films, with an increase in thickness, elongation and permeability to water vapor, and a decrease in the tensile strength and elasticity modulus of the film. The solubility was not significantly affected by the addition of OLE, however the color difference with the addition of OLE was 64.72%. Another important result was the in vivo analysis of psychrophiles, that is, when applied to the packaging of lamb meat, carrageenan films with extract had an inhibitory effect on the count of psychrophiles. The study revealed the benefits of olive leaf extract, obtained by microwave incorporated in films based on carrageenan with potential for application as active packaging. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-12-15T19:05:31Z |
dc.date.available.fl_str_mv |
2021-12-15T19:05:31Z |
dc.date.issued.fl_str_mv |
2021-03-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/23331 |
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http://repositorio.ufsm.br/handle/1/23331 |
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por |
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por |
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300600000006 |
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600 600 600 600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Tecnologia |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Engenharia Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Engenharia Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Tecnologia |
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