Infecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecular
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Manancial - Repositório Digital da UFSM |
Texto Completo: | http://repositorio.ufsm.br/handle/1/24498 |
Resumo: | Parasites from de genus Sarcocystis, Toxoplasma and Neospora which belong to the Apicomplexa phylum cause infection in birds. Birds feeding habits as consumption of food touching the soil enhances the probability of cysts ingestion and because of that they indirectly demonstrate the parasites that are contaminating the environment. Birds are intermediate hosts and when they are killed and ingested they became infection sources to definitive hosts. Studies have showed that these parasites infect birds but the role of birds in protozoa life cycles remains unclear. Therefore, the objective of this study was to check the occurrence and the molecular detection of Sarcocystis spp., Toxoplasma gondii and Neospora caninum in tissues from naturally infected birds. Tissue samples (brains, hearts and chest muscle) were collected from birds destined to necropsy at the Avian Pathologies Diagnosis Central Laboratory (LCDPA) of the Federal University of Santa Maria (UFSM). Birds were free-living, domestic or originating from conservatories or maintainer and died from different causes. Samples were submitted to DNA extraction, PCR amplification of the 18S rRNA gene to Sarcocystis spp., NC5 gene to N. caninum and repetitive gene 529 base pares for T. gondii. Positive samples were sequenced and compared with sequences in GenBank. This thesis was organized in two chapters based on birds’ tissue samples availability to parasites analyses. In chapter one, samples were collected from 65 birds (65 brains and 65 hearts) and analyzed for Sarcocystis spp., T. gondii and N. caninum. N. caninum DNA was detected in two birds (02/65 – 3.07%), in brain sample of a Rupornis magnirostris and in brain and heart samples of a Dendrocygna bicolor. DNA from Sarcocystis spp. was detected in three birds (03/65 – 4.62%), in brain of a Nymphicus hollandicus, brains and hearts of a Amazona aestiva and a Paroaria coronata. T. gondii DNA was not detected in any tissues. No mixed infections were observed. In chapter two, chest muscles were collected from 89 birds which were held in captivity aiming Sarcocystis detection. Five amplified sequences (5.61%) detected in Cyanoliseus patagonus (1), Psittacula krameri (1), Pyrrhura frontalis (2) e Ramphastos dicolorus (1) showed 100% identity with Sarcocystis spp. The diversity of hosts species naturally infected analyzed by molecular methods showed Sarcocystis and Neospora infecting different birds’ species and probably these birds are involved in protozoa epidemiology. |
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2022-05-26T13:02:52Z2022-05-26T13:02:52Z2022-02-18http://repositorio.ufsm.br/handle/1/24498Parasites from de genus Sarcocystis, Toxoplasma and Neospora which belong to the Apicomplexa phylum cause infection in birds. Birds feeding habits as consumption of food touching the soil enhances the probability of cysts ingestion and because of that they indirectly demonstrate the parasites that are contaminating the environment. Birds are intermediate hosts and when they are killed and ingested they became infection sources to definitive hosts. Studies have showed that these parasites infect birds but the role of birds in protozoa life cycles remains unclear. Therefore, the objective of this study was to check the occurrence and the molecular detection of Sarcocystis spp., Toxoplasma gondii and Neospora caninum in tissues from naturally infected birds. Tissue samples (brains, hearts and chest muscle) were collected from birds destined to necropsy at the Avian Pathologies Diagnosis Central Laboratory (LCDPA) of the Federal University of Santa Maria (UFSM). Birds were free-living, domestic or originating from conservatories or maintainer and died from different causes. Samples were submitted to DNA extraction, PCR amplification of the 18S rRNA gene to Sarcocystis spp., NC5 gene to N. caninum and repetitive gene 529 base pares for T. gondii. Positive samples were sequenced and compared with sequences in GenBank. This thesis was organized in two chapters based on birds’ tissue samples availability to parasites analyses. In chapter one, samples were collected from 65 birds (65 brains and 65 hearts) and analyzed for Sarcocystis spp., T. gondii and N. caninum. N. caninum DNA was detected in two birds (02/65 – 3.07%), in brain sample of a Rupornis magnirostris and in brain and heart samples of a Dendrocygna bicolor. DNA from Sarcocystis spp. was detected in three birds (03/65 – 4.62%), in brain of a Nymphicus hollandicus, brains and hearts of a Amazona aestiva and a Paroaria coronata. T. gondii DNA was not detected in any tissues. No mixed infections were observed. In chapter two, chest muscles were collected from 89 birds which were held in captivity aiming Sarcocystis detection. Five amplified sequences (5.61%) detected in Cyanoliseus patagonus (1), Psittacula krameri (1), Pyrrhura frontalis (2) e Ramphastos dicolorus (1) showed 100% identity with Sarcocystis spp. The diversity of hosts species naturally infected analyzed by molecular methods showed Sarcocystis and Neospora infecting different birds’ species and probably these birds are involved in protozoa epidemiology.As aves podem ser infectadas por parasitas dos gêneros Sarcocystis, Toxoplasma e Neospora, pertencentes ao filo Apicomplexa. O hábito de alimentarem-se diretamente do solo aumenta a probabilidade de ingestão de oocistos, sendo importantes sinalizadores dos parasitas que estão contaminando o ambiente. No ciclo biológico podem ser consideradas hospedeiras intermediárias e quando predadas tornam-se fonte de infecção para os hospedeiros definitivos. Embora estudos demonstram que as aves podem ser infectadas por estes parasitas inúmeras espécies continuam sem diagnóstico e a participação no ciclo biológico desses parasitas não está totalmente esclarecido. Assim, o objetivo deste estudo foi verificar a ocorrência e detectar a presença de DNA de Sarcocystis spp., Toxoplasma gondii e Neospora caninum em amostras de tecido de aves naturalmente infectadas. Para isso, as aves recebidas no Laboratório Central de Diagnóstico de Patologias Aviárias (LCDPA-UFSM) foram necropsiadas e realizada a coleta de amostra de tecido (cérebro, coração e músculo do peito). Os animais eram de cativeiro, vida livre e domésticas e morreram por causas variadas. Posteriormente, no Laboratório de Doenças Parasitárias (LADOPAR-UFSM) foi realizada a extração de DNA das amostras de tecido, amplificação pela Reação em Cadeia da Polimerase (PCR) do gene 18S rRNA para Sarcocystis spp., gene NC5 para N. caninum e gene repetitivo 529 pares de bases para T. gondii. As amostras positivas foram purificadas, sequenciadas e comparadas com as sequências depositadas no GenBank. Esta tese foi organizada em dois capítulos conforme a disponibilidade de amostras de tecido de aves para pesquisa dos parasitas. No capítulo 1, foram coletadas amostras de tecido de 65 aves (65 cérebros e 65 corações) de cativeiro, vida livre e domésticas e analisadas para Sarcocystis spp., T. gondii e N. caninum. DNA de Sarcocystis spp. foi detectada em três aves (03/65 - 4,62%), em cérebro de Nymphicus hollandicus, cérebros e corações de Amazona aestiva e Paroaria coronata. O DNA de T. gondii não foi detectado em nenhum tecido. O DNA de N. caninum foi detectado em duas aves (02/65 - 3,07%), em amostra de cérebro de Rupornis magnirostris e em amostras de cérebro e coração de Dendrocygna bicolor. Não foram observadas infecções mistas. No capítulo 2, foram coletadas amostras de músculo do peito de 89 aves de cativeiro para pesquisa de membros do gênero Sarcocystis. Do total, em cinco (5,61%) as sequências amplificadas exibiram 100% de identidade com DNA de Sarcocystis spp., detectadas no músculo do peito de Cyanoliseus patagonus (1), Psittacula krameri (1), Pyrrhura frontalis (2) e Ramphastos dicolorus (1). A expansão da diversidade de espécies hospedeiras naturalmente infectadas analisadas através do desenvolvimento de métodos moleculares levou à detecção de membros dos gêneros Sarcocystis e Neospora em diferentes espécies de aves, demonstrando que provavelmente elas estão envolvidas na epidemiologia destes parasitas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessApicomplexaPCRAvesNaturalmente infectadaApicomplexaBirdsNaturally infectedCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAInfecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecularSarcocystis spp., Toxoplasma gondii and Neospora caninum infection in birds: occurrence and molecular detectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisVogel, Fernanda Silveira Flôreshttp://lattes.cnpq.br/9676833435314493Camillo, GiovanaSantos, Helton Fernandes dosPortella, Luiza PiresBraüning, Patríciahttp://lattes.cnpq.br/7353397234422530Alves, Marta Elena Machado500500000007600600600600600600600a86342d7-ec99-49d4-8748-67e3266c0759cca28a53-8b90-4585-947e-4404656041a9d2862b48-cfed-4a38-bf74-9067cf38cf896d199d56-94d8-451b-bcc2-4bc86b78491f1de7f11f-3310-465f-acd4-8f6f731f1932334bc64d-0adb-408a-a867-dd6c323ea326reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Infecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecular |
dc.title.alternative.eng.fl_str_mv |
Sarcocystis spp., Toxoplasma gondii and Neospora caninum infection in birds: occurrence and molecular detection |
title |
Infecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecular |
spellingShingle |
Infecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecular Alves, Marta Elena Machado Apicomplexa PCR Aves Naturalmente infectada Apicomplexa Birds Naturally infected CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Infecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecular |
title_full |
Infecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecular |
title_fullStr |
Infecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecular |
title_full_unstemmed |
Infecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecular |
title_sort |
Infecção por Sarcocystis spp., Toxoplasma gondii e Neospora caninum em aves: ocorrência e detecção molecular |
author |
Alves, Marta Elena Machado |
author_facet |
Alves, Marta Elena Machado |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vogel, Fernanda Silveira Flôres |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9676833435314493 |
dc.contributor.referee1.fl_str_mv |
Camillo, Giovana |
dc.contributor.referee2.fl_str_mv |
Santos, Helton Fernandes dos |
dc.contributor.referee3.fl_str_mv |
Portella, Luiza Pires |
dc.contributor.referee4.fl_str_mv |
Braüning, Patrícia |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7353397234422530 |
dc.contributor.author.fl_str_mv |
Alves, Marta Elena Machado |
contributor_str_mv |
Vogel, Fernanda Silveira Flôres Camillo, Giovana Santos, Helton Fernandes dos Portella, Luiza Pires Braüning, Patrícia |
dc.subject.por.fl_str_mv |
Apicomplexa PCR Aves Naturalmente infectada |
topic |
Apicomplexa PCR Aves Naturalmente infectada Apicomplexa Birds Naturally infected CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Apicomplexa Birds Naturally infected |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Parasites from de genus Sarcocystis, Toxoplasma and Neospora which belong to the Apicomplexa phylum cause infection in birds. Birds feeding habits as consumption of food touching the soil enhances the probability of cysts ingestion and because of that they indirectly demonstrate the parasites that are contaminating the environment. Birds are intermediate hosts and when they are killed and ingested they became infection sources to definitive hosts. Studies have showed that these parasites infect birds but the role of birds in protozoa life cycles remains unclear. Therefore, the objective of this study was to check the occurrence and the molecular detection of Sarcocystis spp., Toxoplasma gondii and Neospora caninum in tissues from naturally infected birds. Tissue samples (brains, hearts and chest muscle) were collected from birds destined to necropsy at the Avian Pathologies Diagnosis Central Laboratory (LCDPA) of the Federal University of Santa Maria (UFSM). Birds were free-living, domestic or originating from conservatories or maintainer and died from different causes. Samples were submitted to DNA extraction, PCR amplification of the 18S rRNA gene to Sarcocystis spp., NC5 gene to N. caninum and repetitive gene 529 base pares for T. gondii. Positive samples were sequenced and compared with sequences in GenBank. This thesis was organized in two chapters based on birds’ tissue samples availability to parasites analyses. In chapter one, samples were collected from 65 birds (65 brains and 65 hearts) and analyzed for Sarcocystis spp., T. gondii and N. caninum. N. caninum DNA was detected in two birds (02/65 – 3.07%), in brain sample of a Rupornis magnirostris and in brain and heart samples of a Dendrocygna bicolor. DNA from Sarcocystis spp. was detected in three birds (03/65 – 4.62%), in brain of a Nymphicus hollandicus, brains and hearts of a Amazona aestiva and a Paroaria coronata. T. gondii DNA was not detected in any tissues. No mixed infections were observed. In chapter two, chest muscles were collected from 89 birds which were held in captivity aiming Sarcocystis detection. Five amplified sequences (5.61%) detected in Cyanoliseus patagonus (1), Psittacula krameri (1), Pyrrhura frontalis (2) e Ramphastos dicolorus (1) showed 100% identity with Sarcocystis spp. The diversity of hosts species naturally infected analyzed by molecular methods showed Sarcocystis and Neospora infecting different birds’ species and probably these birds are involved in protozoa epidemiology. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-05-26T13:02:52Z |
dc.date.available.fl_str_mv |
2022-05-26T13:02:52Z |
dc.date.issued.fl_str_mv |
2022-02-18 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/24498 |
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http://repositorio.ufsm.br/handle/1/24498 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500500000007 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 600 600 |
dc.relation.authority.fl_str_mv |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
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collection |
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