Degree of moisture in seeds for the cryopreservation of orchids native to Brazil

Detalhes bibliográficos
Autor(a) principal: Ferrari,Edilene Aparecida Preti
Data de Publicação: 2020
Outros Autores: Colombo,Ronan Carlos, Faria,Ricardo Tadeu de, Neves,Carmen Silvia Vieira Janeiro, Vero,Franciele da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000800401
Resumo: ABSTRACT: The objective of this study was to determine the ideal moisture degree for the cryopreservation of Cattleya labiata Lindl. and Miltonia regnellii Rchb.f. orchid seeds. Orchid seeds were standardized in relation to the desired moisture degrees (4, 6, 8, 10, 12, and 15%) and were subjected to rapid freezing in liquid nitrogen (-196 °C) for 24 h; then, the seeds were defrosted and analyzed. For both orchid species, the seeds that were immersed in liquid nitrogen with 4% moisture content had the highest viability percentage, averaging 95% and 68% of viable seeds for C. labiata and M. regnellii, respectively. The seeds with 12% and 15% moisture content had no viable seeds. For the fresh mass of seedlings, there was no significant difference between moisture content treatments for either species.
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spelling Degree of moisture in seeds for the cryopreservation of orchids native to Brazilliquid nitrogenfreezingsurvivalCattleya labiata Lindl.Miltonia regnellii Rchb.f.ABSTRACT: The objective of this study was to determine the ideal moisture degree for the cryopreservation of Cattleya labiata Lindl. and Miltonia regnellii Rchb.f. orchid seeds. Orchid seeds were standardized in relation to the desired moisture degrees (4, 6, 8, 10, 12, and 15%) and were subjected to rapid freezing in liquid nitrogen (-196 °C) for 24 h; then, the seeds were defrosted and analyzed. For both orchid species, the seeds that were immersed in liquid nitrogen with 4% moisture content had the highest viability percentage, averaging 95% and 68% of viable seeds for C. labiata and M. regnellii, respectively. The seeds with 12% and 15% moisture content had no viable seeds. For the fresh mass of seedlings, there was no significant difference between moisture content treatments for either species.Universidade Federal de Santa Maria2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000800401Ciência Rural v.50 n.8 2020reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20180498info:eu-repo/semantics/openAccessFerrari,Edilene Aparecida PretiColombo,Ronan CarlosFaria,Ricardo Tadeu deNeves,Carmen Silvia Vieira JaneiroVero,Franciele da Silvaeng2020-08-10T00:00:00ZRevista
dc.title.none.fl_str_mv Degree of moisture in seeds for the cryopreservation of orchids native to Brazil
title Degree of moisture in seeds for the cryopreservation of orchids native to Brazil
spellingShingle Degree of moisture in seeds for the cryopreservation of orchids native to Brazil
Ferrari,Edilene Aparecida Preti
liquid nitrogen
freezing
survival
Cattleya labiata Lindl.
Miltonia regnellii Rchb.f.
title_short Degree of moisture in seeds for the cryopreservation of orchids native to Brazil
title_full Degree of moisture in seeds for the cryopreservation of orchids native to Brazil
title_fullStr Degree of moisture in seeds for the cryopreservation of orchids native to Brazil
title_full_unstemmed Degree of moisture in seeds for the cryopreservation of orchids native to Brazil
title_sort Degree of moisture in seeds for the cryopreservation of orchids native to Brazil
author Ferrari,Edilene Aparecida Preti
author_facet Ferrari,Edilene Aparecida Preti
Colombo,Ronan Carlos
Faria,Ricardo Tadeu de
Neves,Carmen Silvia Vieira Janeiro
Vero,Franciele da Silva
author_role author
author2 Colombo,Ronan Carlos
Faria,Ricardo Tadeu de
Neves,Carmen Silvia Vieira Janeiro
Vero,Franciele da Silva
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ferrari,Edilene Aparecida Preti
Colombo,Ronan Carlos
Faria,Ricardo Tadeu de
Neves,Carmen Silvia Vieira Janeiro
Vero,Franciele da Silva
dc.subject.por.fl_str_mv liquid nitrogen
freezing
survival
Cattleya labiata Lindl.
Miltonia regnellii Rchb.f.
topic liquid nitrogen
freezing
survival
Cattleya labiata Lindl.
Miltonia regnellii Rchb.f.
description ABSTRACT: The objective of this study was to determine the ideal moisture degree for the cryopreservation of Cattleya labiata Lindl. and Miltonia regnellii Rchb.f. orchid seeds. Orchid seeds were standardized in relation to the desired moisture degrees (4, 6, 8, 10, 12, and 15%) and were subjected to rapid freezing in liquid nitrogen (-196 °C) for 24 h; then, the seeds were defrosted and analyzed. For both orchid species, the seeds that were immersed in liquid nitrogen with 4% moisture content had the highest viability percentage, averaging 95% and 68% of viable seeds for C. labiata and M. regnellii, respectively. The seeds with 12% and 15% moisture content had no viable seeds. For the fresh mass of seedlings, there was no significant difference between moisture content treatments for either species.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000800401
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000800401
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20180498
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.50 n.8 2020
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140554838441984

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