Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis

Detalhes bibliográficos
Autor(a) principal: Nunes,Ana Rita Fonseca
Data de Publicação: 2010
Outros Autores: Martins,Juliana Nascimento, Furlaneto,Márcia Cristina, Barros,Neiva Monteiro de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000900001
Resumo: Nomuraea rileyi represents an important natural control agent of Anticarsia gemmatalis preventing populations from reaching economic threshold levels in soybean. During the processes of host infection, entomopathogenic fungi produce extracellular proteases, which degrade the host cuticle and are suggested to be virulence determinants. It was examined the production of subtilisin-like (Pr1) and trypsin-like (Pr2) proteases in two strains (NR458 and CG434) of N. rileyi and its possible role in the process of pathogenicity to this caterpillar. Fungal growth was performed in a mineral medium containing nitrate, and supplemented with the cuticle or exuviae from A. gemmatalis, or with the non-cuticular substrate casein. In medium containing nitrate as sole nitrogen source, no detectable Pr1-like activity occurred in the culture supernatants of the two fungal strains. However, both strains of N. rileyi produced Pr1-like protease in all medium amended with exogenous nitrogen source, and it was highly expressed in the presence of insect cuticle. Pr2-like activity was significantly inferior to Pr1-like activity and it was detected only in some of the media culture and incubation periods tested. In the NR458 culture supernatant the highest activity was observed in medium containing nitrate as nitrogen source. Correlation analysis between the percentage of A. gemmatalis mortality in bioassays and Pr1-like protease activity of strain NR458 suggests a positive correlation for these variables.
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spelling Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalisbiological controlsubtilisin-like [Pr1]trypsin-like [Pr2]Nomuraea rileyi represents an important natural control agent of Anticarsia gemmatalis preventing populations from reaching economic threshold levels in soybean. During the processes of host infection, entomopathogenic fungi produce extracellular proteases, which degrade the host cuticle and are suggested to be virulence determinants. It was examined the production of subtilisin-like (Pr1) and trypsin-like (Pr2) proteases in two strains (NR458 and CG434) of N. rileyi and its possible role in the process of pathogenicity to this caterpillar. Fungal growth was performed in a mineral medium containing nitrate, and supplemented with the cuticle or exuviae from A. gemmatalis, or with the non-cuticular substrate casein. In medium containing nitrate as sole nitrogen source, no detectable Pr1-like activity occurred in the culture supernatants of the two fungal strains. However, both strains of N. rileyi produced Pr1-like protease in all medium amended with exogenous nitrogen source, and it was highly expressed in the presence of insect cuticle. Pr2-like activity was significantly inferior to Pr1-like activity and it was detected only in some of the media culture and incubation periods tested. In the NR458 culture supernatant the highest activity was observed in medium containing nitrate as nitrogen source. Correlation analysis between the percentage of A. gemmatalis mortality in bioassays and Pr1-like protease activity of strain NR458 suggests a positive correlation for these variables.Universidade Federal de Santa Maria2010-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000900001Ciência Rural v.40 n.9 2010reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782010005000149info:eu-repo/semantics/openAccessNunes,Ana Rita FonsecaMartins,Juliana NascimentoFurlaneto,Márcia CristinaBarros,Neiva Monteiro deeng2010-10-22T00:00:00ZRevista
dc.title.none.fl_str_mv Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
title Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
spellingShingle Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
Nunes,Ana Rita Fonseca
biological control
subtilisin-like [Pr1]
trypsin-like [Pr2]
title_short Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
title_full Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
title_fullStr Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
title_full_unstemmed Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
title_sort Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
author Nunes,Ana Rita Fonseca
author_facet Nunes,Ana Rita Fonseca
Martins,Juliana Nascimento
Furlaneto,Márcia Cristina
Barros,Neiva Monteiro de
author_role author
author2 Martins,Juliana Nascimento
Furlaneto,Márcia Cristina
Barros,Neiva Monteiro de
author2_role author
author
author
dc.contributor.author.fl_str_mv Nunes,Ana Rita Fonseca
Martins,Juliana Nascimento
Furlaneto,Márcia Cristina
Barros,Neiva Monteiro de
dc.subject.por.fl_str_mv biological control
subtilisin-like [Pr1]
trypsin-like [Pr2]
topic biological control
subtilisin-like [Pr1]
trypsin-like [Pr2]
description Nomuraea rileyi represents an important natural control agent of Anticarsia gemmatalis preventing populations from reaching economic threshold levels in soybean. During the processes of host infection, entomopathogenic fungi produce extracellular proteases, which degrade the host cuticle and are suggested to be virulence determinants. It was examined the production of subtilisin-like (Pr1) and trypsin-like (Pr2) proteases in two strains (NR458 and CG434) of N. rileyi and its possible role in the process of pathogenicity to this caterpillar. Fungal growth was performed in a mineral medium containing nitrate, and supplemented with the cuticle or exuviae from A. gemmatalis, or with the non-cuticular substrate casein. In medium containing nitrate as sole nitrogen source, no detectable Pr1-like activity occurred in the culture supernatants of the two fungal strains. However, both strains of N. rileyi produced Pr1-like protease in all medium amended with exogenous nitrogen source, and it was highly expressed in the presence of insect cuticle. Pr2-like activity was significantly inferior to Pr1-like activity and it was detected only in some of the media culture and incubation periods tested. In the NR458 culture supernatant the highest activity was observed in medium containing nitrate as nitrogen source. Correlation analysis between the percentage of A. gemmatalis mortality in bioassays and Pr1-like protease activity of strain NR458 suggests a positive correlation for these variables.
publishDate 2010
dc.date.none.fl_str_mv 2010-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000900001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000900001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782010005000149
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.40 n.9 2010
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
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repository.mail.fl_str_mv
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