Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000900001 |
Resumo: | Nomuraea rileyi represents an important natural control agent of Anticarsia gemmatalis preventing populations from reaching economic threshold levels in soybean. During the processes of host infection, entomopathogenic fungi produce extracellular proteases, which degrade the host cuticle and are suggested to be virulence determinants. It was examined the production of subtilisin-like (Pr1) and trypsin-like (Pr2) proteases in two strains (NR458 and CG434) of N. rileyi and its possible role in the process of pathogenicity to this caterpillar. Fungal growth was performed in a mineral medium containing nitrate, and supplemented with the cuticle or exuviae from A. gemmatalis, or with the non-cuticular substrate casein. In medium containing nitrate as sole nitrogen source, no detectable Pr1-like activity occurred in the culture supernatants of the two fungal strains. However, both strains of N. rileyi produced Pr1-like protease in all medium amended with exogenous nitrogen source, and it was highly expressed in the presence of insect cuticle. Pr2-like activity was significantly inferior to Pr1-like activity and it was detected only in some of the media culture and incubation periods tested. In the NR458 culture supernatant the highest activity was observed in medium containing nitrate as nitrogen source. Correlation analysis between the percentage of A. gemmatalis mortality in bioassays and Pr1-like protease activity of strain NR458 suggests a positive correlation for these variables. |
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Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalisbiological controlsubtilisin-like [Pr1]trypsin-like [Pr2]Nomuraea rileyi represents an important natural control agent of Anticarsia gemmatalis preventing populations from reaching economic threshold levels in soybean. During the processes of host infection, entomopathogenic fungi produce extracellular proteases, which degrade the host cuticle and are suggested to be virulence determinants. It was examined the production of subtilisin-like (Pr1) and trypsin-like (Pr2) proteases in two strains (NR458 and CG434) of N. rileyi and its possible role in the process of pathogenicity to this caterpillar. Fungal growth was performed in a mineral medium containing nitrate, and supplemented with the cuticle or exuviae from A. gemmatalis, or with the non-cuticular substrate casein. In medium containing nitrate as sole nitrogen source, no detectable Pr1-like activity occurred in the culture supernatants of the two fungal strains. However, both strains of N. rileyi produced Pr1-like protease in all medium amended with exogenous nitrogen source, and it was highly expressed in the presence of insect cuticle. Pr2-like activity was significantly inferior to Pr1-like activity and it was detected only in some of the media culture and incubation periods tested. In the NR458 culture supernatant the highest activity was observed in medium containing nitrate as nitrogen source. Correlation analysis between the percentage of A. gemmatalis mortality in bioassays and Pr1-like protease activity of strain NR458 suggests a positive correlation for these variables.Universidade Federal de Santa Maria2010-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000900001Ciência Rural v.40 n.9 2010reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782010005000149info:eu-repo/semantics/openAccessNunes,Ana Rita FonsecaMartins,Juliana NascimentoFurlaneto,Márcia CristinaBarros,Neiva Monteiro deeng2010-10-22T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis |
title |
Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis |
spellingShingle |
Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis Nunes,Ana Rita Fonseca biological control subtilisin-like [Pr1] trypsin-like [Pr2] |
title_short |
Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis |
title_full |
Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis |
title_fullStr |
Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis |
title_full_unstemmed |
Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis |
title_sort |
Production of cuticle-degrading proteases by Nomuraea rileyi and its virulence against Anticarsia gemmatalis |
author |
Nunes,Ana Rita Fonseca |
author_facet |
Nunes,Ana Rita Fonseca Martins,Juliana Nascimento Furlaneto,Márcia Cristina Barros,Neiva Monteiro de |
author_role |
author |
author2 |
Martins,Juliana Nascimento Furlaneto,Márcia Cristina Barros,Neiva Monteiro de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Nunes,Ana Rita Fonseca Martins,Juliana Nascimento Furlaneto,Márcia Cristina Barros,Neiva Monteiro de |
dc.subject.por.fl_str_mv |
biological control subtilisin-like [Pr1] trypsin-like [Pr2] |
topic |
biological control subtilisin-like [Pr1] trypsin-like [Pr2] |
description |
Nomuraea rileyi represents an important natural control agent of Anticarsia gemmatalis preventing populations from reaching economic threshold levels in soybean. During the processes of host infection, entomopathogenic fungi produce extracellular proteases, which degrade the host cuticle and are suggested to be virulence determinants. It was examined the production of subtilisin-like (Pr1) and trypsin-like (Pr2) proteases in two strains (NR458 and CG434) of N. rileyi and its possible role in the process of pathogenicity to this caterpillar. Fungal growth was performed in a mineral medium containing nitrate, and supplemented with the cuticle or exuviae from A. gemmatalis, or with the non-cuticular substrate casein. In medium containing nitrate as sole nitrogen source, no detectable Pr1-like activity occurred in the culture supernatants of the two fungal strains. However, both strains of N. rileyi produced Pr1-like protease in all medium amended with exogenous nitrogen source, and it was highly expressed in the presence of insect cuticle. Pr2-like activity was significantly inferior to Pr1-like activity and it was detected only in some of the media culture and incubation periods tested. In the NR458 culture supernatant the highest activity was observed in medium containing nitrate as nitrogen source. Correlation analysis between the percentage of A. gemmatalis mortality in bioassays and Pr1-like protease activity of strain NR458 suggests a positive correlation for these variables. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000900001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000900001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782010005000149 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.40 n.9 2010 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140537783353344 |