Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea

Detalhes bibliográficos
Autor(a) principal: Paula,Cleber Jacob Silva de
Data de Publicação: 2008
Outros Autores: Marin,José Moacir
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000600029
Resumo: Shiga toxigenic Escherichia coli (STEC) and Attaching and effacing E. coli (AEEC) have been associated with diarrhea illness in dogs. From January to December 2006, 92 E. coli isolates from 25 diarrheic dogs were analyzed, by screening for the presence of Shiga toxin-producing (stx 1 and stx 2) and intimin (eae) genes. Twelve isolates were detected by PCR to harbor the Shiga toxin genes (7 the stx 1 (7.6%); 5 the stx 2 (5.4%); and none both of them). Nine (9.8%) of the E. coli isolates studied were eae positive non Shiga toxin-producing. Thirteen (62.0%) isolates, carrying stx or eae gene, also showed a hemolysin production. The strains with virulence genes were also examined for resistance to 12 antimicrobial agents. Resistances to cephalothin (85.7%), streptomycin (81.0%), amoxicillin (71.4%) and gentamicin (71.4%) were predominantly observed.
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spelling Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrheaEscherichia coliSTECeae geneantimicrobial resistanceShiga toxigenic Escherichia coli (STEC) and Attaching and effacing E. coli (AEEC) have been associated with diarrhea illness in dogs. From January to December 2006, 92 E. coli isolates from 25 diarrheic dogs were analyzed, by screening for the presence of Shiga toxin-producing (stx 1 and stx 2) and intimin (eae) genes. Twelve isolates were detected by PCR to harbor the Shiga toxin genes (7 the stx 1 (7.6%); 5 the stx 2 (5.4%); and none both of them). Nine (9.8%) of the E. coli isolates studied were eae positive non Shiga toxin-producing. Thirteen (62.0%) isolates, carrying stx or eae gene, also showed a hemolysin production. The strains with virulence genes were also examined for resistance to 12 antimicrobial agents. Resistances to cephalothin (85.7%), streptomycin (81.0%), amoxicillin (71.4%) and gentamicin (71.4%) were predominantly observed.Universidade Federal de Santa Maria2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000600029Ciência Rural v.38 n.6 2008reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782008000600029info:eu-repo/semantics/openAccessPaula,Cleber Jacob Silva deMarin,José Moacireng2008-11-11T00:00:00ZRevista
dc.title.none.fl_str_mv Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
title Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
spellingShingle Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
Paula,Cleber Jacob Silva de
Escherichia coli
STEC
eae gene
antimicrobial resistance
title_short Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
title_full Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
title_fullStr Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
title_full_unstemmed Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
title_sort Occurrence of non-O157 Shiga toxin-producing Escherichia coli in dogs with diarrhea
author Paula,Cleber Jacob Silva de
author_facet Paula,Cleber Jacob Silva de
Marin,José Moacir
author_role author
author2 Marin,José Moacir
author2_role author
dc.contributor.author.fl_str_mv Paula,Cleber Jacob Silva de
Marin,José Moacir
dc.subject.por.fl_str_mv Escherichia coli
STEC
eae gene
antimicrobial resistance
topic Escherichia coli
STEC
eae gene
antimicrobial resistance
description Shiga toxigenic Escherichia coli (STEC) and Attaching and effacing E. coli (AEEC) have been associated with diarrhea illness in dogs. From January to December 2006, 92 E. coli isolates from 25 diarrheic dogs were analyzed, by screening for the presence of Shiga toxin-producing (stx 1 and stx 2) and intimin (eae) genes. Twelve isolates were detected by PCR to harbor the Shiga toxin genes (7 the stx 1 (7.6%); 5 the stx 2 (5.4%); and none both of them). Nine (9.8%) of the E. coli isolates studied were eae positive non Shiga toxin-producing. Thirteen (62.0%) isolates, carrying stx or eae gene, also showed a hemolysin production. The strains with virulence genes were also examined for resistance to 12 antimicrobial agents. Resistances to cephalothin (85.7%), streptomycin (81.0%), amoxicillin (71.4%) and gentamicin (71.4%) were predominantly observed.
publishDate 2008
dc.date.none.fl_str_mv 2008-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000600029
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000600029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782008000600029
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.38 n.6 2008
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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