B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001200452 |
Resumo: | ABSTRACT: Feline leukemia virus (FeLV) causes an infection in cats that, in some cases, can also be reported with other pathologies, such as infection with feline immunodeficiency virus (FIV), feline infectious peritonitis (FIP), and lymphoma. Although, a compromised immune response is reported in these animals, little is known about the immunological state of their cells. To shed some light in this area, we studied peripheral blood samples from both infected and non-infected cats with FeLV, with or without FIV, FIP, and lymphoma. We tested a panel of monoclonal antibodies (n=11) against mouse and human antigens and we reported that cat leukocytes can be stained with anti-mouse B220 monoclonal antibody; therefore, percentages of B cells were evaluated in different cat groups. Our results showed that cats with FeLV and FIP, or with leukemia, presented a large decrease in B220+ mononuclear cells. However, FeLV+ cats without clinical signs, or with unspecific clinical signs, had the same amount of B220+ mononuclear cells as healthy cats (control cats). Since the expression of B220 is exclusively restricted to the naïve B cell population, we inferred that the absence of these B cells in FeLV+ cats is related to other conditions that affect B cell numbers, such as viral infections and leukemias. Therefore, the amount of naïve B cells in peripheral blood (i.e., B220+ cells) can be used to identify FeLV+ cats concomitantly carrying FIP or leukemia, from FeLV+ cats with lymphoma or without any clinical signs. |
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B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying catsB cellsFeLVFIVFIPlymphomaABSTRACT: Feline leukemia virus (FeLV) causes an infection in cats that, in some cases, can also be reported with other pathologies, such as infection with feline immunodeficiency virus (FIV), feline infectious peritonitis (FIP), and lymphoma. Although, a compromised immune response is reported in these animals, little is known about the immunological state of their cells. To shed some light in this area, we studied peripheral blood samples from both infected and non-infected cats with FeLV, with or without FIV, FIP, and lymphoma. We tested a panel of monoclonal antibodies (n=11) against mouse and human antigens and we reported that cat leukocytes can be stained with anti-mouse B220 monoclonal antibody; therefore, percentages of B cells were evaluated in different cat groups. Our results showed that cats with FeLV and FIP, or with leukemia, presented a large decrease in B220+ mononuclear cells. However, FeLV+ cats without clinical signs, or with unspecific clinical signs, had the same amount of B220+ mononuclear cells as healthy cats (control cats). Since the expression of B220 is exclusively restricted to the naïve B cell population, we inferred that the absence of these B cells in FeLV+ cats is related to other conditions that affect B cell numbers, such as viral infections and leukemias. Therefore, the amount of naïve B cells in peripheral blood (i.e., B220+ cells) can be used to identify FeLV+ cats concomitantly carrying FIP or leukemia, from FeLV+ cats with lymphoma or without any clinical signs.Universidade Federal de Santa Maria2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001200452Ciência Rural v.50 n.12 2020reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20190467info:eu-repo/semantics/openAccessFrota,Isadora Duarte SantosSouza,Jéssica de OliveiraBusato,Fernanda de OliveiraAraujo,Cristina Abreu deCurbani,FlávioOlivieri,DavidTadokoro,Carlos Eduardoeng2020-10-20T00:00:00ZRevista |
dc.title.none.fl_str_mv |
B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats |
title |
B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats |
spellingShingle |
B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats Frota,Isadora Duarte Santos B cells FeLV FIV FIP lymphoma |
title_short |
B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats |
title_full |
B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats |
title_fullStr |
B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats |
title_full_unstemmed |
B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats |
title_sort |
B220 expression as an immunological marker for differentiation of Feline Leukemia Virus carrying cats |
author |
Frota,Isadora Duarte Santos |
author_facet |
Frota,Isadora Duarte Santos Souza,Jéssica de Oliveira Busato,Fernanda de Oliveira Araujo,Cristina Abreu de Curbani,Flávio Olivieri,David Tadokoro,Carlos Eduardo |
author_role |
author |
author2 |
Souza,Jéssica de Oliveira Busato,Fernanda de Oliveira Araujo,Cristina Abreu de Curbani,Flávio Olivieri,David Tadokoro,Carlos Eduardo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Frota,Isadora Duarte Santos Souza,Jéssica de Oliveira Busato,Fernanda de Oliveira Araujo,Cristina Abreu de Curbani,Flávio Olivieri,David Tadokoro,Carlos Eduardo |
dc.subject.por.fl_str_mv |
B cells FeLV FIV FIP lymphoma |
topic |
B cells FeLV FIV FIP lymphoma |
description |
ABSTRACT: Feline leukemia virus (FeLV) causes an infection in cats that, in some cases, can also be reported with other pathologies, such as infection with feline immunodeficiency virus (FIV), feline infectious peritonitis (FIP), and lymphoma. Although, a compromised immune response is reported in these animals, little is known about the immunological state of their cells. To shed some light in this area, we studied peripheral blood samples from both infected and non-infected cats with FeLV, with or without FIV, FIP, and lymphoma. We tested a panel of monoclonal antibodies (n=11) against mouse and human antigens and we reported that cat leukocytes can be stained with anti-mouse B220 monoclonal antibody; therefore, percentages of B cells were evaluated in different cat groups. Our results showed that cats with FeLV and FIP, or with leukemia, presented a large decrease in B220+ mononuclear cells. However, FeLV+ cats without clinical signs, or with unspecific clinical signs, had the same amount of B220+ mononuclear cells as healthy cats (control cats). Since the expression of B220 is exclusively restricted to the naïve B cell population, we inferred that the absence of these B cells in FeLV+ cats is related to other conditions that affect B cell numbers, such as viral infections and leukemias. Therefore, the amount of naïve B cells in peripheral blood (i.e., B220+ cells) can be used to identify FeLV+ cats concomitantly carrying FIP or leukemia, from FeLV+ cats with lymphoma or without any clinical signs. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001200452 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001200452 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20190467 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.50 n.12 2020 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140555230609408 |