Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display

Detalhes bibliográficos
Autor(a) principal: Ribeiro,Eduardo Lara
Data de Publicação: 2021
Outros Autores: Fujimura,Patrícia Tiemi, Ueira-Vieira,Carlos, Goulart,Luiz Ricardo, Alves,Telma Maria, Lage,Andrey Pereira, Heinemann,Marcos Bryan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000800455
Resumo: ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.
id UFSM-2_15e378195db93639214ace4890123c39
oai_identifier_str oai:scielo:S0103-84782021000800455
network_acronym_str UFSM-2
network_name_str Ciência rural (Online)
repository_id_str
spelling Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage displayCampylobacteriosisphage displaypeptidesABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.Universidade Federal de Santa Maria2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000800455Ciência Rural v.51 n.8 2021reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20200945info:eu-repo/semantics/openAccessRibeiro,Eduardo LaraFujimura,Patrícia TiemiUeira-Vieira,CarlosGoulart,Luiz RicardoAlves,Telma MariaLage,Andrey PereiraHeinemann,Marcos Bryaneng2021-06-09T00:00:00ZRevista
dc.title.none.fl_str_mv Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display
title Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display
spellingShingle Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display
Ribeiro,Eduardo Lara
Campylobacteriosis
phage display
peptides
title_short Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display
title_full Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display
title_fullStr Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display
title_full_unstemmed Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display
title_sort Selection and characterization of peptides mimetic to Campylobacter fetus subsp. venerealis using phage display
author Ribeiro,Eduardo Lara
author_facet Ribeiro,Eduardo Lara
Fujimura,Patrícia Tiemi
Ueira-Vieira,Carlos
Goulart,Luiz Ricardo
Alves,Telma Maria
Lage,Andrey Pereira
Heinemann,Marcos Bryan
author_role author
author2 Fujimura,Patrícia Tiemi
Ueira-Vieira,Carlos
Goulart,Luiz Ricardo
Alves,Telma Maria
Lage,Andrey Pereira
Heinemann,Marcos Bryan
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro,Eduardo Lara
Fujimura,Patrícia Tiemi
Ueira-Vieira,Carlos
Goulart,Luiz Ricardo
Alves,Telma Maria
Lage,Andrey Pereira
Heinemann,Marcos Bryan
dc.subject.por.fl_str_mv Campylobacteriosis
phage display
peptides
topic Campylobacteriosis
phage display
peptides
description ABSTRACT: Bovine genital campylobacteriosis (BGC) is a venereal and subclinical disease that affects the fertility of cattle herds, and it is caused by Campylobacter fetus subsp. venerealis . This study selected peptides mimetic to the BGC-causing agent from a phage library. Phage display is a technique that applies bacteriophage libraries that reveal peptides fused to the viral capsid in biological selections against target proteins. Biopannings were performed for biological selection in the phage library using rabbit hyperimmune serum and C. fetus subsp. venerealis protein extract. Five selected heptapeptides were considered mimetic to Cfv-NCTC 10354 based on the results of bioinformatics analysis and assays with hyperimmune serum and cervicovaginal mucus obtained from heifers. ALASLPL and LSYLFPP were the most reactive peptides and considered promising as possible mimetic immunogens for C. fetus subsp. venerealis.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000800455
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000800455
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20200945
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.51 n.8 2021
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140556088344576

Registros relacionados