Development and maturation of the lung in fetuses of Galea spixii and expression of markers
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901635 |
Resumo: | ABSTRACT: The aim of this research was to study the development of the lung in Galea spixii , by gross anatomy, histological analysis and immunohistochemical techniques. Totally, 8 fetuses were used and allocated into three groups of age: Group I (33-35 days), Group II (38-40 days), and Group III (43-45 days) with given crown-rump - CR lenghts. According to the gross morphology, there were no differences in relation to the lung morphology among groups. In relation to lung maturation, the Group I showed early formation of the bronchi and bronchioles, which were richly surrounded by mesenchyme and small blood vessels, typical features of the pseudoglandular stage. Individuals from Group II showed higher amounts of tubular formations in the lung parenchyma and reduced mesenchyme, reaching the canalicular stage. The lung from individuals of the Group III was completely formed, reaching the alveolar phase. In the immunohistochemical analysis, the lung of individuals from both Groups I and II were positive for Pcna, Oct-4, and VEGF. In contrast, there was not labbeling on samples from Group III. Thus, the G. spixii lung anatomy resembles the agouti ( Dasyprocta sp.) lung, in relation to the number of lobes and fissures. In regard to the lung development, G. spixii showed more similarity with the human fetal lung, since both are born in the stage of the alveolar phase, different to other rodent species. |
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Development and maturation of the lung in fetuses of Galea spixii and expression of markersrespiratory systemalveolimorphologyrodentexperimental model.ABSTRACT: The aim of this research was to study the development of the lung in Galea spixii , by gross anatomy, histological analysis and immunohistochemical techniques. Totally, 8 fetuses were used and allocated into three groups of age: Group I (33-35 days), Group II (38-40 days), and Group III (43-45 days) with given crown-rump - CR lenghts. According to the gross morphology, there were no differences in relation to the lung morphology among groups. In relation to lung maturation, the Group I showed early formation of the bronchi and bronchioles, which were richly surrounded by mesenchyme and small blood vessels, typical features of the pseudoglandular stage. Individuals from Group II showed higher amounts of tubular formations in the lung parenchyma and reduced mesenchyme, reaching the canalicular stage. The lung from individuals of the Group III was completely formed, reaching the alveolar phase. In the immunohistochemical analysis, the lung of individuals from both Groups I and II were positive for Pcna, Oct-4, and VEGF. In contrast, there was not labbeling on samples from Group III. Thus, the G. spixii lung anatomy resembles the agouti ( Dasyprocta sp.) lung, in relation to the number of lobes and fissures. In regard to the lung development, G. spixii showed more similarity with the human fetal lung, since both are born in the stage of the alveolar phase, different to other rodent species.Universidade Federal de Santa Maria2016-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901635Ciência Rural v.46 n.9 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20150711info:eu-repo/semantics/openAccessFavaron,Phelipe OliveiraOliveira,Moacir Franco deBorghesi,JéssicaAnunciação,Adriana Raquel de AlmeidaMiglino,Maria Angelicaeng2016-08-12T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Development and maturation of the lung in fetuses of Galea spixii and expression of markers |
title |
Development and maturation of the lung in fetuses of Galea spixii and expression of markers |
spellingShingle |
Development and maturation of the lung in fetuses of Galea spixii and expression of markers Favaron,Phelipe Oliveira respiratory system alveoli morphology rodent experimental model. |
title_short |
Development and maturation of the lung in fetuses of Galea spixii and expression of markers |
title_full |
Development and maturation of the lung in fetuses of Galea spixii and expression of markers |
title_fullStr |
Development and maturation of the lung in fetuses of Galea spixii and expression of markers |
title_full_unstemmed |
Development and maturation of the lung in fetuses of Galea spixii and expression of markers |
title_sort |
Development and maturation of the lung in fetuses of Galea spixii and expression of markers |
author |
Favaron,Phelipe Oliveira |
author_facet |
Favaron,Phelipe Oliveira Oliveira,Moacir Franco de Borghesi,Jéssica Anunciação,Adriana Raquel de Almeida Miglino,Maria Angelica |
author_role |
author |
author2 |
Oliveira,Moacir Franco de Borghesi,Jéssica Anunciação,Adriana Raquel de Almeida Miglino,Maria Angelica |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Favaron,Phelipe Oliveira Oliveira,Moacir Franco de Borghesi,Jéssica Anunciação,Adriana Raquel de Almeida Miglino,Maria Angelica |
dc.subject.por.fl_str_mv |
respiratory system alveoli morphology rodent experimental model. |
topic |
respiratory system alveoli morphology rodent experimental model. |
description |
ABSTRACT: The aim of this research was to study the development of the lung in Galea spixii , by gross anatomy, histological analysis and immunohistochemical techniques. Totally, 8 fetuses were used and allocated into three groups of age: Group I (33-35 days), Group II (38-40 days), and Group III (43-45 days) with given crown-rump - CR lenghts. According to the gross morphology, there were no differences in relation to the lung morphology among groups. In relation to lung maturation, the Group I showed early formation of the bronchi and bronchioles, which were richly surrounded by mesenchyme and small blood vessels, typical features of the pseudoglandular stage. Individuals from Group II showed higher amounts of tubular formations in the lung parenchyma and reduced mesenchyme, reaching the canalicular stage. The lung from individuals of the Group III was completely formed, reaching the alveolar phase. In the immunohistochemical analysis, the lung of individuals from both Groups I and II were positive for Pcna, Oct-4, and VEGF. In contrast, there was not labbeling on samples from Group III. Thus, the G. spixii lung anatomy resembles the agouti ( Dasyprocta sp.) lung, in relation to the number of lobes and fissures. In regard to the lung development, G. spixii showed more similarity with the human fetal lung, since both are born in the stage of the alveolar phase, different to other rodent species. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901635 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000901635 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20150711 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.46 n.9 2016 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140550463782912 |