Three superovulation protocols for in vivo embryo production in Santa Inês sheep

Detalhes bibliográficos
Autor(a) principal: Arashiro,Eduardo Kenji Nunes
Data de Publicação: 2022
Outros Autores: Pinto,Pedro Henrique Nicolau, Lima,Julia Timponi Moura, Henry,Marc, Ascoli,Fábio Otero, Souza-Fabjan,Joanna Maria Gonçalves, Fonseca,Jeferson Ferreira Da, Brandão,Felipe Zandonadi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000800701
Resumo: ABSTRACT: There is still no consensus regarding the best protocol for in vivo embryo production in sheep despite increasing studies in this area. Moreover, there is variability in the response of ewes to superovulation (SOV). An approach to mitigate this inconsistency is to initiate gonadotropin administration under favorable ovarian conditions. The present study compared three treatments in a crossover design: a traditional SOV protocol (TRAD) and “Day 0” D0 SOV protocol with (D0+GnRH), or without Lecilerin (D0-GnRH). Fifteen Santa Inês ewes received 200 mg of FSH at six decreasing doses and PGF2α with the fifth dose of FSH. They were naturally mated with fertile rams and subjected to surgical embryo collection. The number of viable embryos was similar among the different treatments (TRAD = 6.0 ± 4.7; D0-GnRH = 3.8 ± 6.4; D0+GnRH = 7.5 ± 6.5). Regardless of the treatment method, ewes with follicles ≤ 4 mm, at the first FSH dose, produced more viable embryos (9.6 ± 6.0, P < 0.05) compared to ewes that had follicles > 4 mm at the beginning of the SOV (2.9 ± 3.1, viable embryos). Both the TRAD and D0+GnRH groups had fewer animals with large follicles (> 4 mm) at the first FSH dose than the D0-GnRH group (P < 0.05). In conclusion, both the TRAD and D0+GnRH treatments induced a more favorable ovarian condition (follicles ≤ 4 mm) for adequate SOV; although, all three treatments exhibited similar efficacies in Santa Inês sheep.
id UFSM-2_3ed4c10ddf1a0a7f30addafe2eaedbb3
oai_identifier_str oai:scielo:S0103-84782022000800701
network_acronym_str UFSM-2
network_name_str Ciência rural (Online)
repository_id_str
spelling Three superovulation protocols for in vivo embryo production in Santa Inês sheepday 0 protocolewefollicular populationGnRHMOETABSTRACT: There is still no consensus regarding the best protocol for in vivo embryo production in sheep despite increasing studies in this area. Moreover, there is variability in the response of ewes to superovulation (SOV). An approach to mitigate this inconsistency is to initiate gonadotropin administration under favorable ovarian conditions. The present study compared three treatments in a crossover design: a traditional SOV protocol (TRAD) and “Day 0” D0 SOV protocol with (D0+GnRH), or without Lecilerin (D0-GnRH). Fifteen Santa Inês ewes received 200 mg of FSH at six decreasing doses and PGF2α with the fifth dose of FSH. They were naturally mated with fertile rams and subjected to surgical embryo collection. The number of viable embryos was similar among the different treatments (TRAD = 6.0 ± 4.7; D0-GnRH = 3.8 ± 6.4; D0+GnRH = 7.5 ± 6.5). Regardless of the treatment method, ewes with follicles ≤ 4 mm, at the first FSH dose, produced more viable embryos (9.6 ± 6.0, P < 0.05) compared to ewes that had follicles > 4 mm at the beginning of the SOV (2.9 ± 3.1, viable embryos). Both the TRAD and D0+GnRH groups had fewer animals with large follicles (> 4 mm) at the first FSH dose than the D0-GnRH group (P < 0.05). In conclusion, both the TRAD and D0+GnRH treatments induced a more favorable ovarian condition (follicles ≤ 4 mm) for adequate SOV; although, all three treatments exhibited similar efficacies in Santa Inês sheep.Universidade Federal de Santa Maria2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000800701Ciência Rural v.52 n.8 2022reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20210308info:eu-repo/semantics/openAccessArashiro,Eduardo Kenji NunesPinto,Pedro Henrique NicolauLima,Julia Timponi MouraHenry,MarcAscoli,Fábio OteroSouza-Fabjan,Joanna Maria GonçalvesFonseca,Jeferson Ferreira DaBrandão,Felipe Zandonadieng2021-12-21T00:00:00ZRevista
dc.title.none.fl_str_mv Three superovulation protocols for in vivo embryo production in Santa Inês sheep
title Three superovulation protocols for in vivo embryo production in Santa Inês sheep
spellingShingle Three superovulation protocols for in vivo embryo production in Santa Inês sheep
Arashiro,Eduardo Kenji Nunes
day 0 protocol
ewe
follicular population
GnRH
MOET
title_short Three superovulation protocols for in vivo embryo production in Santa Inês sheep
title_full Three superovulation protocols for in vivo embryo production in Santa Inês sheep
title_fullStr Three superovulation protocols for in vivo embryo production in Santa Inês sheep
title_full_unstemmed Three superovulation protocols for in vivo embryo production in Santa Inês sheep
title_sort Three superovulation protocols for in vivo embryo production in Santa Inês sheep
author Arashiro,Eduardo Kenji Nunes
author_facet Arashiro,Eduardo Kenji Nunes
Pinto,Pedro Henrique Nicolau
Lima,Julia Timponi Moura
Henry,Marc
Ascoli,Fábio Otero
Souza-Fabjan,Joanna Maria Gonçalves
Fonseca,Jeferson Ferreira Da
Brandão,Felipe Zandonadi
author_role author
author2 Pinto,Pedro Henrique Nicolau
Lima,Julia Timponi Moura
Henry,Marc
Ascoli,Fábio Otero
Souza-Fabjan,Joanna Maria Gonçalves
Fonseca,Jeferson Ferreira Da
Brandão,Felipe Zandonadi
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Arashiro,Eduardo Kenji Nunes
Pinto,Pedro Henrique Nicolau
Lima,Julia Timponi Moura
Henry,Marc
Ascoli,Fábio Otero
Souza-Fabjan,Joanna Maria Gonçalves
Fonseca,Jeferson Ferreira Da
Brandão,Felipe Zandonadi
dc.subject.por.fl_str_mv day 0 protocol
ewe
follicular population
GnRH
MOET
topic day 0 protocol
ewe
follicular population
GnRH
MOET
description ABSTRACT: There is still no consensus regarding the best protocol for in vivo embryo production in sheep despite increasing studies in this area. Moreover, there is variability in the response of ewes to superovulation (SOV). An approach to mitigate this inconsistency is to initiate gonadotropin administration under favorable ovarian conditions. The present study compared three treatments in a crossover design: a traditional SOV protocol (TRAD) and “Day 0” D0 SOV protocol with (D0+GnRH), or without Lecilerin (D0-GnRH). Fifteen Santa Inês ewes received 200 mg of FSH at six decreasing doses and PGF2α with the fifth dose of FSH. They were naturally mated with fertile rams and subjected to surgical embryo collection. The number of viable embryos was similar among the different treatments (TRAD = 6.0 ± 4.7; D0-GnRH = 3.8 ± 6.4; D0+GnRH = 7.5 ± 6.5). Regardless of the treatment method, ewes with follicles ≤ 4 mm, at the first FSH dose, produced more viable embryos (9.6 ± 6.0, P < 0.05) compared to ewes that had follicles > 4 mm at the beginning of the SOV (2.9 ± 3.1, viable embryos). Both the TRAD and D0+GnRH groups had fewer animals with large follicles (> 4 mm) at the first FSH dose than the D0-GnRH group (P < 0.05). In conclusion, both the TRAD and D0+GnRH treatments induced a more favorable ovarian condition (follicles ≤ 4 mm) for adequate SOV; although, all three treatments exhibited similar efficacies in Santa Inês sheep.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000800701
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000800701
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20210308
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.52 n.8 2022
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140557008994304