Three superovulation protocols for in vivo embryo production in Santa Inês sheep
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000800701 |
Resumo: | ABSTRACT: There is still no consensus regarding the best protocol for in vivo embryo production in sheep despite increasing studies in this area. Moreover, there is variability in the response of ewes to superovulation (SOV). An approach to mitigate this inconsistency is to initiate gonadotropin administration under favorable ovarian conditions. The present study compared three treatments in a crossover design: a traditional SOV protocol (TRAD) and “Day 0” D0 SOV protocol with (D0+GnRH), or without Lecilerin (D0-GnRH). Fifteen Santa Inês ewes received 200 mg of FSH at six decreasing doses and PGF2α with the fifth dose of FSH. They were naturally mated with fertile rams and subjected to surgical embryo collection. The number of viable embryos was similar among the different treatments (TRAD = 6.0 ± 4.7; D0-GnRH = 3.8 ± 6.4; D0+GnRH = 7.5 ± 6.5). Regardless of the treatment method, ewes with follicles ≤ 4 mm, at the first FSH dose, produced more viable embryos (9.6 ± 6.0, P < 0.05) compared to ewes that had follicles > 4 mm at the beginning of the SOV (2.9 ± 3.1, viable embryos). Both the TRAD and D0+GnRH groups had fewer animals with large follicles (> 4 mm) at the first FSH dose than the D0-GnRH group (P < 0.05). In conclusion, both the TRAD and D0+GnRH treatments induced a more favorable ovarian condition (follicles ≤ 4 mm) for adequate SOV; although, all three treatments exhibited similar efficacies in Santa Inês sheep. |
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Ciência rural (Online) |
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Three superovulation protocols for in vivo embryo production in Santa Inês sheepday 0 protocolewefollicular populationGnRHMOETABSTRACT: There is still no consensus regarding the best protocol for in vivo embryo production in sheep despite increasing studies in this area. Moreover, there is variability in the response of ewes to superovulation (SOV). An approach to mitigate this inconsistency is to initiate gonadotropin administration under favorable ovarian conditions. The present study compared three treatments in a crossover design: a traditional SOV protocol (TRAD) and “Day 0” D0 SOV protocol with (D0+GnRH), or without Lecilerin (D0-GnRH). Fifteen Santa Inês ewes received 200 mg of FSH at six decreasing doses and PGF2α with the fifth dose of FSH. They were naturally mated with fertile rams and subjected to surgical embryo collection. The number of viable embryos was similar among the different treatments (TRAD = 6.0 ± 4.7; D0-GnRH = 3.8 ± 6.4; D0+GnRH = 7.5 ± 6.5). Regardless of the treatment method, ewes with follicles ≤ 4 mm, at the first FSH dose, produced more viable embryos (9.6 ± 6.0, P < 0.05) compared to ewes that had follicles > 4 mm at the beginning of the SOV (2.9 ± 3.1, viable embryos). Both the TRAD and D0+GnRH groups had fewer animals with large follicles (> 4 mm) at the first FSH dose than the D0-GnRH group (P < 0.05). In conclusion, both the TRAD and D0+GnRH treatments induced a more favorable ovarian condition (follicles ≤ 4 mm) for adequate SOV; although, all three treatments exhibited similar efficacies in Santa Inês sheep.Universidade Federal de Santa Maria2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000800701Ciência Rural v.52 n.8 2022reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20210308info:eu-repo/semantics/openAccessArashiro,Eduardo Kenji NunesPinto,Pedro Henrique NicolauLima,Julia Timponi MouraHenry,MarcAscoli,Fábio OteroSouza-Fabjan,Joanna Maria GonçalvesFonseca,Jeferson Ferreira DaBrandão,Felipe Zandonadieng2021-12-21T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Three superovulation protocols for in vivo embryo production in Santa Inês sheep |
title |
Three superovulation protocols for in vivo embryo production in Santa Inês sheep |
spellingShingle |
Three superovulation protocols for in vivo embryo production in Santa Inês sheep Arashiro,Eduardo Kenji Nunes day 0 protocol ewe follicular population GnRH MOET |
title_short |
Three superovulation protocols for in vivo embryo production in Santa Inês sheep |
title_full |
Three superovulation protocols for in vivo embryo production in Santa Inês sheep |
title_fullStr |
Three superovulation protocols for in vivo embryo production in Santa Inês sheep |
title_full_unstemmed |
Three superovulation protocols for in vivo embryo production in Santa Inês sheep |
title_sort |
Three superovulation protocols for in vivo embryo production in Santa Inês sheep |
author |
Arashiro,Eduardo Kenji Nunes |
author_facet |
Arashiro,Eduardo Kenji Nunes Pinto,Pedro Henrique Nicolau Lima,Julia Timponi Moura Henry,Marc Ascoli,Fábio Otero Souza-Fabjan,Joanna Maria Gonçalves Fonseca,Jeferson Ferreira Da Brandão,Felipe Zandonadi |
author_role |
author |
author2 |
Pinto,Pedro Henrique Nicolau Lima,Julia Timponi Moura Henry,Marc Ascoli,Fábio Otero Souza-Fabjan,Joanna Maria Gonçalves Fonseca,Jeferson Ferreira Da Brandão,Felipe Zandonadi |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Arashiro,Eduardo Kenji Nunes Pinto,Pedro Henrique Nicolau Lima,Julia Timponi Moura Henry,Marc Ascoli,Fábio Otero Souza-Fabjan,Joanna Maria Gonçalves Fonseca,Jeferson Ferreira Da Brandão,Felipe Zandonadi |
dc.subject.por.fl_str_mv |
day 0 protocol ewe follicular population GnRH MOET |
topic |
day 0 protocol ewe follicular population GnRH MOET |
description |
ABSTRACT: There is still no consensus regarding the best protocol for in vivo embryo production in sheep despite increasing studies in this area. Moreover, there is variability in the response of ewes to superovulation (SOV). An approach to mitigate this inconsistency is to initiate gonadotropin administration under favorable ovarian conditions. The present study compared three treatments in a crossover design: a traditional SOV protocol (TRAD) and “Day 0” D0 SOV protocol with (D0+GnRH), or without Lecilerin (D0-GnRH). Fifteen Santa Inês ewes received 200 mg of FSH at six decreasing doses and PGF2α with the fifth dose of FSH. They were naturally mated with fertile rams and subjected to surgical embryo collection. The number of viable embryos was similar among the different treatments (TRAD = 6.0 ± 4.7; D0-GnRH = 3.8 ± 6.4; D0+GnRH = 7.5 ± 6.5). Regardless of the treatment method, ewes with follicles ≤ 4 mm, at the first FSH dose, produced more viable embryos (9.6 ± 6.0, P < 0.05) compared to ewes that had follicles > 4 mm at the beginning of the SOV (2.9 ± 3.1, viable embryos). Both the TRAD and D0+GnRH groups had fewer animals with large follicles (> 4 mm) at the first FSH dose than the D0-GnRH group (P < 0.05). In conclusion, both the TRAD and D0+GnRH treatments induced a more favorable ovarian condition (follicles ≤ 4 mm) for adequate SOV; although, all three treatments exhibited similar efficacies in Santa Inês sheep. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000800701 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000800701 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20210308 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.52 n.8 2022 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140557008994304 |