Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?

Detalhes bibliográficos
Autor(a) principal: Fêo,Haline Ballestero
Data de Publicação: 2020
Outros Autores: Flórez,Luis Mauricio Montoya, Yamatogi,Ricardo Seiti, Duzanski,Anderson do Prado, Araújo Junior,João Pessoa, Oliveira,Rogerio Antonio de, Rocha,Noeme Sousa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001100603
Resumo: ABSTRACT: Canine transmissible venereal tumor (CTVT) is a transmissible neoplasm, which spreads naturally between dogs through the halogenic transfer of tumor cells, mainly during coitus. It is the oldest known tumoral lineage in nature and reports on gene mutations have been extended. Also, this tumor shares several genetic mutations with some cancers in humans, among them lung carcinomas, melanoma, prostate, breast, among other cancers. Thus, expression of tumor suppressor genes such as TP53, P21, and apoptosis-related genes such as BAX, BCL-2, and BCL-xL, both in vivo and in vitro (primary cell culture) were quantified. In the present study, the comparison of gene expression, the TP53 gene, in most cases, was shown to be high in the majority of tissues (65%) and primary cell culture (100%), while BCL-2, BCL-xL, and BAX presented variation among the animals analyzed. Moreover, in these situations, the results suggested that the apoptotic regulation of these genes did not occur for TP53. The P21 gene was shown to be mostly normal (70%); although, absence (6%) and underexpressions (24%) were also observed. Statistical analysis of the BCL-xL gene demonstrated significant differences between the tissues of the animals when compared to the cell cultures; however, to the other genes, no statistical difference was observed between the groups. Preliminarily, the results suggested the presence of alterations in the gene expressions of the TP53, P21, BAX, BCL-2 and BCL-xL leading to loss of function in these genes, which affect the tumorigenesis of CTVT.
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spelling Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?cancerapoptosisgenetic alterationscell culturesABSTRACT: Canine transmissible venereal tumor (CTVT) is a transmissible neoplasm, which spreads naturally between dogs through the halogenic transfer of tumor cells, mainly during coitus. It is the oldest known tumoral lineage in nature and reports on gene mutations have been extended. Also, this tumor shares several genetic mutations with some cancers in humans, among them lung carcinomas, melanoma, prostate, breast, among other cancers. Thus, expression of tumor suppressor genes such as TP53, P21, and apoptosis-related genes such as BAX, BCL-2, and BCL-xL, both in vivo and in vitro (primary cell culture) were quantified. In the present study, the comparison of gene expression, the TP53 gene, in most cases, was shown to be high in the majority of tissues (65%) and primary cell culture (100%), while BCL-2, BCL-xL, and BAX presented variation among the animals analyzed. Moreover, in these situations, the results suggested that the apoptotic regulation of these genes did not occur for TP53. The P21 gene was shown to be mostly normal (70%); although, absence (6%) and underexpressions (24%) were also observed. Statistical analysis of the BCL-xL gene demonstrated significant differences between the tissues of the animals when compared to the cell cultures; however, to the other genes, no statistical difference was observed between the groups. Preliminarily, the results suggested the presence of alterations in the gene expressions of the TP53, P21, BAX, BCL-2 and BCL-xL leading to loss of function in these genes, which affect the tumorigenesis of CTVT.Universidade Federal de Santa Maria2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001100603Ciência Rural v.50 n.11 2020reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20200082info:eu-repo/semantics/openAccessFêo,Haline BallesteroFlórez,Luis Mauricio MontoyaYamatogi,Ricardo SeitiDuzanski,Anderson do PradoAraújo Junior,João PessoaOliveira,Rogerio Antonio deRocha,Noeme Sousaeng2020-10-05T00:00:00ZRevista
dc.title.none.fl_str_mv Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?
title Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?
spellingShingle Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?
Fêo,Haline Ballestero
cancer
apoptosis
genetic alterations
cell cultures
title_short Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?
title_full Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?
title_fullStr Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?
title_full_unstemmed Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?
title_sort Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?
author Fêo,Haline Ballestero
author_facet Fêo,Haline Ballestero
Flórez,Luis Mauricio Montoya
Yamatogi,Ricardo Seiti
Duzanski,Anderson do Prado
Araújo Junior,João Pessoa
Oliveira,Rogerio Antonio de
Rocha,Noeme Sousa
author_role author
author2 Flórez,Luis Mauricio Montoya
Yamatogi,Ricardo Seiti
Duzanski,Anderson do Prado
Araújo Junior,João Pessoa
Oliveira,Rogerio Antonio de
Rocha,Noeme Sousa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fêo,Haline Ballestero
Flórez,Luis Mauricio Montoya
Yamatogi,Ricardo Seiti
Duzanski,Anderson do Prado
Araújo Junior,João Pessoa
Oliveira,Rogerio Antonio de
Rocha,Noeme Sousa
dc.subject.por.fl_str_mv cancer
apoptosis
genetic alterations
cell cultures
topic cancer
apoptosis
genetic alterations
cell cultures
description ABSTRACT: Canine transmissible venereal tumor (CTVT) is a transmissible neoplasm, which spreads naturally between dogs through the halogenic transfer of tumor cells, mainly during coitus. It is the oldest known tumoral lineage in nature and reports on gene mutations have been extended. Also, this tumor shares several genetic mutations with some cancers in humans, among them lung carcinomas, melanoma, prostate, breast, among other cancers. Thus, expression of tumor suppressor genes such as TP53, P21, and apoptosis-related genes such as BAX, BCL-2, and BCL-xL, both in vivo and in vitro (primary cell culture) were quantified. In the present study, the comparison of gene expression, the TP53 gene, in most cases, was shown to be high in the majority of tissues (65%) and primary cell culture (100%), while BCL-2, BCL-xL, and BAX presented variation among the animals analyzed. Moreover, in these situations, the results suggested that the apoptotic regulation of these genes did not occur for TP53. The P21 gene was shown to be mostly normal (70%); although, absence (6%) and underexpressions (24%) were also observed. Statistical analysis of the BCL-xL gene demonstrated significant differences between the tissues of the animals when compared to the cell cultures; however, to the other genes, no statistical difference was observed between the groups. Preliminarily, the results suggested the presence of alterations in the gene expressions of the TP53, P21, BAX, BCL-2 and BCL-xL leading to loss of function in these genes, which affect the tumorigenesis of CTVT.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001100603
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001100603
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20200082
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.50 n.11 2020
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
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repository.mail.fl_str_mv
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