Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001100603 |
Resumo: | ABSTRACT: Canine transmissible venereal tumor (CTVT) is a transmissible neoplasm, which spreads naturally between dogs through the halogenic transfer of tumor cells, mainly during coitus. It is the oldest known tumoral lineage in nature and reports on gene mutations have been extended. Also, this tumor shares several genetic mutations with some cancers in humans, among them lung carcinomas, melanoma, prostate, breast, among other cancers. Thus, expression of tumor suppressor genes such as TP53, P21, and apoptosis-related genes such as BAX, BCL-2, and BCL-xL, both in vivo and in vitro (primary cell culture) were quantified. In the present study, the comparison of gene expression, the TP53 gene, in most cases, was shown to be high in the majority of tissues (65%) and primary cell culture (100%), while BCL-2, BCL-xL, and BAX presented variation among the animals analyzed. Moreover, in these situations, the results suggested that the apoptotic regulation of these genes did not occur for TP53. The P21 gene was shown to be mostly normal (70%); although, absence (6%) and underexpressions (24%) were also observed. Statistical analysis of the BCL-xL gene demonstrated significant differences between the tissues of the animals when compared to the cell cultures; however, to the other genes, no statistical difference was observed between the groups. Preliminarily, the results suggested the presence of alterations in the gene expressions of the TP53, P21, BAX, BCL-2 and BCL-xL leading to loss of function in these genes, which affect the tumorigenesis of CTVT. |
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Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs?cancerapoptosisgenetic alterationscell culturesABSTRACT: Canine transmissible venereal tumor (CTVT) is a transmissible neoplasm, which spreads naturally between dogs through the halogenic transfer of tumor cells, mainly during coitus. It is the oldest known tumoral lineage in nature and reports on gene mutations have been extended. Also, this tumor shares several genetic mutations with some cancers in humans, among them lung carcinomas, melanoma, prostate, breast, among other cancers. Thus, expression of tumor suppressor genes such as TP53, P21, and apoptosis-related genes such as BAX, BCL-2, and BCL-xL, both in vivo and in vitro (primary cell culture) were quantified. In the present study, the comparison of gene expression, the TP53 gene, in most cases, was shown to be high in the majority of tissues (65%) and primary cell culture (100%), while BCL-2, BCL-xL, and BAX presented variation among the animals analyzed. Moreover, in these situations, the results suggested that the apoptotic regulation of these genes did not occur for TP53. The P21 gene was shown to be mostly normal (70%); although, absence (6%) and underexpressions (24%) were also observed. Statistical analysis of the BCL-xL gene demonstrated significant differences between the tissues of the animals when compared to the cell cultures; however, to the other genes, no statistical difference was observed between the groups. Preliminarily, the results suggested the presence of alterations in the gene expressions of the TP53, P21, BAX, BCL-2 and BCL-xL leading to loss of function in these genes, which affect the tumorigenesis of CTVT.Universidade Federal de Santa Maria2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001100603Ciência Rural v.50 n.11 2020reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20200082info:eu-repo/semantics/openAccessFêo,Haline BallesteroFlórez,Luis Mauricio MontoyaYamatogi,Ricardo SeitiDuzanski,Anderson do PradoAraújo Junior,João PessoaOliveira,Rogerio Antonio deRocha,Noeme Sousaeng2020-10-05T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs? |
title |
Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs? |
spellingShingle |
Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs? Fêo,Haline Ballestero cancer apoptosis genetic alterations cell cultures |
title_short |
Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs? |
title_full |
Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs? |
title_fullStr |
Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs? |
title_full_unstemmed |
Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs? |
title_sort |
Do alterations in gene expressions influence tumorigenesis in the transmissible venereal tumor in dogs? |
author |
Fêo,Haline Ballestero |
author_facet |
Fêo,Haline Ballestero Flórez,Luis Mauricio Montoya Yamatogi,Ricardo Seiti Duzanski,Anderson do Prado Araújo Junior,João Pessoa Oliveira,Rogerio Antonio de Rocha,Noeme Sousa |
author_role |
author |
author2 |
Flórez,Luis Mauricio Montoya Yamatogi,Ricardo Seiti Duzanski,Anderson do Prado Araújo Junior,João Pessoa Oliveira,Rogerio Antonio de Rocha,Noeme Sousa |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fêo,Haline Ballestero Flórez,Luis Mauricio Montoya Yamatogi,Ricardo Seiti Duzanski,Anderson do Prado Araújo Junior,João Pessoa Oliveira,Rogerio Antonio de Rocha,Noeme Sousa |
dc.subject.por.fl_str_mv |
cancer apoptosis genetic alterations cell cultures |
topic |
cancer apoptosis genetic alterations cell cultures |
description |
ABSTRACT: Canine transmissible venereal tumor (CTVT) is a transmissible neoplasm, which spreads naturally between dogs through the halogenic transfer of tumor cells, mainly during coitus. It is the oldest known tumoral lineage in nature and reports on gene mutations have been extended. Also, this tumor shares several genetic mutations with some cancers in humans, among them lung carcinomas, melanoma, prostate, breast, among other cancers. Thus, expression of tumor suppressor genes such as TP53, P21, and apoptosis-related genes such as BAX, BCL-2, and BCL-xL, both in vivo and in vitro (primary cell culture) were quantified. In the present study, the comparison of gene expression, the TP53 gene, in most cases, was shown to be high in the majority of tissues (65%) and primary cell culture (100%), while BCL-2, BCL-xL, and BAX presented variation among the animals analyzed. Moreover, in these situations, the results suggested that the apoptotic regulation of these genes did not occur for TP53. The P21 gene was shown to be mostly normal (70%); although, absence (6%) and underexpressions (24%) were also observed. Statistical analysis of the BCL-xL gene demonstrated significant differences between the tissues of the animals when compared to the cell cultures; however, to the other genes, no statistical difference was observed between the groups. Preliminarily, the results suggested the presence of alterations in the gene expressions of the TP53, P21, BAX, BCL-2 and BCL-xL leading to loss of function in these genes, which affect the tumorigenesis of CTVT. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001100603 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020001100603 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20200082 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.50 n.11 2020 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140555202297856 |