Influence of culture media in viability test of conidia of entomopathogenic fungi
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400043 |
Resumo: | This work aimed at investigatimg whether the culture medium used in viability tests affects the conidial germination of Lecanicillium lecanii, Beauveria bassiana and Paecilomyces fumosoroseus isolates. The tests were performed on microscope slides containing one of the culture media: agar-water (AW), minimal medium (MM), potato-dextrose agar (PDA), potato-dextrose-1% yeast extract agar (PDAY), Sabouraud-dextrose-yeast extract agar (SDAY), and complete medium (CM). Three areas per slide were delimited and 0.05ml of a 5.5 x 105 conidia ml-1 suspension was applied to each area. One bioassay was performed for each isolate. Germination was determined after 15 hours of incubation at 26±0.5°C. The culture media influenced the germination of the species studied, verifying within and inter specific variations. CM and PDA provided the highest germination of L. lecanii isolates and the lowest was obtained on SDAY and AW. The germination of B. bassiana isolates was favoured by CM, PDA and PDAY media, a fact not observed in AW and MM. P. fumosoroseus isolates showed the highest germination on CM and PDA media and the lowest on SDAY. However, some isolates presented high germination on nutrient-poor media (AW and MM). |
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Influence of culture media in viability test of conidia of entomopathogenic fungiBeauveria bassianaLecanicillium lecaniiPaecilomyces fumosoroseusgerminationmicrobial controlThis work aimed at investigatimg whether the culture medium used in viability tests affects the conidial germination of Lecanicillium lecanii, Beauveria bassiana and Paecilomyces fumosoroseus isolates. The tests were performed on microscope slides containing one of the culture media: agar-water (AW), minimal medium (MM), potato-dextrose agar (PDA), potato-dextrose-1% yeast extract agar (PDAY), Sabouraud-dextrose-yeast extract agar (SDAY), and complete medium (CM). Three areas per slide were delimited and 0.05ml of a 5.5 x 105 conidia ml-1 suspension was applied to each area. One bioassay was performed for each isolate. Germination was determined after 15 hours of incubation at 26±0.5°C. The culture media influenced the germination of the species studied, verifying within and inter specific variations. CM and PDA provided the highest germination of L. lecanii isolates and the lowest was obtained on SDAY and AW. The germination of B. bassiana isolates was favoured by CM, PDA and PDAY media, a fact not observed in AW and MM. P. fumosoroseus isolates showed the highest germination on CM and PDA media and the lowest on SDAY. However, some isolates presented high germination on nutrient-poor media (AW and MM).Universidade Federal de Santa Maria2006-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400043Ciência Rural v.36 n.4 2006reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782006000400043info:eu-repo/semantics/openAccessFrancisco,Edimara AparecidaMochi,Dinalva AlvesCorreia,Antônia do Carmo BarcelosMonteiro,Antonio Carloseng2006-07-03T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Influence of culture media in viability test of conidia of entomopathogenic fungi |
title |
Influence of culture media in viability test of conidia of entomopathogenic fungi |
spellingShingle |
Influence of culture media in viability test of conidia of entomopathogenic fungi Francisco,Edimara Aparecida Beauveria bassiana Lecanicillium lecanii Paecilomyces fumosoroseus germination microbial control |
title_short |
Influence of culture media in viability test of conidia of entomopathogenic fungi |
title_full |
Influence of culture media in viability test of conidia of entomopathogenic fungi |
title_fullStr |
Influence of culture media in viability test of conidia of entomopathogenic fungi |
title_full_unstemmed |
Influence of culture media in viability test of conidia of entomopathogenic fungi |
title_sort |
Influence of culture media in viability test of conidia of entomopathogenic fungi |
author |
Francisco,Edimara Aparecida |
author_facet |
Francisco,Edimara Aparecida Mochi,Dinalva Alves Correia,Antônia do Carmo Barcelos Monteiro,Antonio Carlos |
author_role |
author |
author2 |
Mochi,Dinalva Alves Correia,Antônia do Carmo Barcelos Monteiro,Antonio Carlos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Francisco,Edimara Aparecida Mochi,Dinalva Alves Correia,Antônia do Carmo Barcelos Monteiro,Antonio Carlos |
dc.subject.por.fl_str_mv |
Beauveria bassiana Lecanicillium lecanii Paecilomyces fumosoroseus germination microbial control |
topic |
Beauveria bassiana Lecanicillium lecanii Paecilomyces fumosoroseus germination microbial control |
description |
This work aimed at investigatimg whether the culture medium used in viability tests affects the conidial germination of Lecanicillium lecanii, Beauveria bassiana and Paecilomyces fumosoroseus isolates. The tests were performed on microscope slides containing one of the culture media: agar-water (AW), minimal medium (MM), potato-dextrose agar (PDA), potato-dextrose-1% yeast extract agar (PDAY), Sabouraud-dextrose-yeast extract agar (SDAY), and complete medium (CM). Three areas per slide were delimited and 0.05ml of a 5.5 x 105 conidia ml-1 suspension was applied to each area. One bioassay was performed for each isolate. Germination was determined after 15 hours of incubation at 26±0.5°C. The culture media influenced the germination of the species studied, verifying within and inter specific variations. CM and PDA provided the highest germination of L. lecanii isolates and the lowest was obtained on SDAY and AW. The germination of B. bassiana isolates was favoured by CM, PDA and PDAY media, a fact not observed in AW and MM. P. fumosoroseus isolates showed the highest germination on CM and PDA media and the lowest on SDAY. However, some isolates presented high germination on nutrient-poor media (AW and MM). |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400043 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000400043 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782006000400043 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.36 n.4 2006 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140527941419008 |