Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021 |
Resumo: | The objective was to evaluate if supplemental LH given at the end of FSH treatment would synchronize the time of ovulation and increase the ovulation rate and embryo yield in Santa Ines ewes. Twenty superovulatory (SOV) programs were accomplished in cross-over design (60d interval). On D0, a CIDR device was inserted, and the device was replaced with a new one 7 days later, when 37.5µg of d-cloprostenol was administered. On D12, we started the SOV treatment, administering 256mg of pFSH 8 times, 12h apart. On D14, the CIDR was removed, and 200IU of eCG and 37.5µg of d-cloprostenol were administered. On D15, the ewes were allocated into one of two groups, a Control group (n=10) that received no supplemental LH and a LH group (n=10) treated with 7.5mg of LH 24h after CIDR removal. Artificial inseminations (AI) were performed 42 and 48h after CIDR removal. The ovarian structures were evaluated by laparoscopy immediately before each AI and 5 days later (D21) when the embryos were collected. The LH ewes ovulated more frequently (P=0.05) before 42h than between 42 and 48h. Treatment with LH tended to increase the frequency of CL and to decrease the anovulatory follicles (P=0.08). The supplemental LH increased the frequency of ewes with a high SOV response (≥11 CL; P=0.05). In conclusion, supplemental LH increased the frequency of ewes with high SOV response and ovulating prior to 42h, however, there was no synchrony between ovulations. The supplemental LH also decreased the frequency of anovulatory follicles, although the ovulation rate and embryo yield were unaffected. |
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Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?superovulationLHovulationembryosheepThe objective was to evaluate if supplemental LH given at the end of FSH treatment would synchronize the time of ovulation and increase the ovulation rate and embryo yield in Santa Ines ewes. Twenty superovulatory (SOV) programs were accomplished in cross-over design (60d interval). On D0, a CIDR device was inserted, and the device was replaced with a new one 7 days later, when 37.5µg of d-cloprostenol was administered. On D12, we started the SOV treatment, administering 256mg of pFSH 8 times, 12h apart. On D14, the CIDR was removed, and 200IU of eCG and 37.5µg of d-cloprostenol were administered. On D15, the ewes were allocated into one of two groups, a Control group (n=10) that received no supplemental LH and a LH group (n=10) treated with 7.5mg of LH 24h after CIDR removal. Artificial inseminations (AI) were performed 42 and 48h after CIDR removal. The ovarian structures were evaluated by laparoscopy immediately before each AI and 5 days later (D21) when the embryos were collected. The LH ewes ovulated more frequently (P=0.05) before 42h than between 42 and 48h. Treatment with LH tended to increase the frequency of CL and to decrease the anovulatory follicles (P=0.08). The supplemental LH increased the frequency of ewes with a high SOV response (≥11 CL; P=0.05). In conclusion, supplemental LH increased the frequency of ewes with high SOV response and ovulating prior to 42h, however, there was no synchrony between ovulations. The supplemental LH also decreased the frequency of anovulatory follicles, although the ovulation rate and embryo yield were unaffected.Universidade Federal de Santa Maria2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021Ciência Rural v.42 n.6 2012reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782012000600021info:eu-repo/semantics/openAccessOliveira,Maria Emilia FrancoCordeiro,Mabel FreitasFerreira,Roberta MachadoSouza,Samuel FigueirêdoPieroni,Juliana Souza PintoRodrigues,Luiz Fernando de SouzaFonseca,Jeferson Ferreira daVicente,Wilter Ricardo Russianoeng2012-07-13T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG? |
title |
Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG? |
spellingShingle |
Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG? Oliveira,Maria Emilia Franco superovulation LH ovulation embryo sheep |
title_short |
Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG? |
title_full |
Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG? |
title_fullStr |
Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG? |
title_full_unstemmed |
Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG? |
title_sort |
Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG? |
author |
Oliveira,Maria Emilia Franco |
author_facet |
Oliveira,Maria Emilia Franco Cordeiro,Mabel Freitas Ferreira,Roberta Machado Souza,Samuel Figueirêdo Pieroni,Juliana Souza Pinto Rodrigues,Luiz Fernando de Souza Fonseca,Jeferson Ferreira da Vicente,Wilter Ricardo Russiano |
author_role |
author |
author2 |
Cordeiro,Mabel Freitas Ferreira,Roberta Machado Souza,Samuel Figueirêdo Pieroni,Juliana Souza Pinto Rodrigues,Luiz Fernando de Souza Fonseca,Jeferson Ferreira da Vicente,Wilter Ricardo Russiano |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Maria Emilia Franco Cordeiro,Mabel Freitas Ferreira,Roberta Machado Souza,Samuel Figueirêdo Pieroni,Juliana Souza Pinto Rodrigues,Luiz Fernando de Souza Fonseca,Jeferson Ferreira da Vicente,Wilter Ricardo Russiano |
dc.subject.por.fl_str_mv |
superovulation LH ovulation embryo sheep |
topic |
superovulation LH ovulation embryo sheep |
description |
The objective was to evaluate if supplemental LH given at the end of FSH treatment would synchronize the time of ovulation and increase the ovulation rate and embryo yield in Santa Ines ewes. Twenty superovulatory (SOV) programs were accomplished in cross-over design (60d interval). On D0, a CIDR device was inserted, and the device was replaced with a new one 7 days later, when 37.5µg of d-cloprostenol was administered. On D12, we started the SOV treatment, administering 256mg of pFSH 8 times, 12h apart. On D14, the CIDR was removed, and 200IU of eCG and 37.5µg of d-cloprostenol were administered. On D15, the ewes were allocated into one of two groups, a Control group (n=10) that received no supplemental LH and a LH group (n=10) treated with 7.5mg of LH 24h after CIDR removal. Artificial inseminations (AI) were performed 42 and 48h after CIDR removal. The ovarian structures were evaluated by laparoscopy immediately before each AI and 5 days later (D21) when the embryos were collected. The LH ewes ovulated more frequently (P=0.05) before 42h than between 42 and 48h. Treatment with LH tended to increase the frequency of CL and to decrease the anovulatory follicles (P=0.08). The supplemental LH increased the frequency of ewes with a high SOV response (≥11 CL; P=0.05). In conclusion, supplemental LH increased the frequency of ewes with high SOV response and ovulating prior to 42h, however, there was no synchrony between ovulations. The supplemental LH also decreased the frequency of anovulatory follicles, although the ovulation rate and embryo yield were unaffected. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782012000600021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.42 n.6 2012 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140541017161728 |