Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?

Detalhes bibliográficos
Autor(a) principal: Oliveira,Maria Emilia Franco
Data de Publicação: 2012
Outros Autores: Cordeiro,Mabel Freitas, Ferreira,Roberta Machado, Souza,Samuel Figueirêdo, Pieroni,Juliana Souza Pinto, Rodrigues,Luiz Fernando de Souza, Fonseca,Jeferson Ferreira da, Vicente,Wilter Ricardo Russiano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021
Resumo: The objective was to evaluate if supplemental LH given at the end of FSH treatment would synchronize the time of ovulation and increase the ovulation rate and embryo yield in Santa Ines ewes. Twenty superovulatory (SOV) programs were accomplished in cross-over design (60d interval). On D0, a CIDR device was inserted, and the device was replaced with a new one 7 days later, when 37.5µg of d-cloprostenol was administered. On D12, we started the SOV treatment, administering 256mg of pFSH 8 times, 12h apart. On D14, the CIDR was removed, and 200IU of eCG and 37.5µg of d-cloprostenol were administered. On D15, the ewes were allocated into one of two groups, a Control group (n=10) that received no supplemental LH and a LH group (n=10) treated with 7.5mg of LH 24h after CIDR removal. Artificial inseminations (AI) were performed 42 and 48h after CIDR removal. The ovarian structures were evaluated by laparoscopy immediately before each AI and 5 days later (D21) when the embryos were collected. The LH ewes ovulated more frequently (P=0.05) before 42h than between 42 and 48h. Treatment with LH tended to increase the frequency of CL and to decrease the anovulatory follicles (P=0.08). The supplemental LH increased the frequency of ewes with a high SOV response (≥11 CL; P=0.05). In conclusion, supplemental LH increased the frequency of ewes with high SOV response and ovulating prior to 42h, however, there was no synchrony between ovulations. The supplemental LH also decreased the frequency of anovulatory follicles, although the ovulation rate and embryo yield were unaffected.
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spelling Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?superovulationLHovulationembryosheepThe objective was to evaluate if supplemental LH given at the end of FSH treatment would synchronize the time of ovulation and increase the ovulation rate and embryo yield in Santa Ines ewes. Twenty superovulatory (SOV) programs were accomplished in cross-over design (60d interval). On D0, a CIDR device was inserted, and the device was replaced with a new one 7 days later, when 37.5µg of d-cloprostenol was administered. On D12, we started the SOV treatment, administering 256mg of pFSH 8 times, 12h apart. On D14, the CIDR was removed, and 200IU of eCG and 37.5µg of d-cloprostenol were administered. On D15, the ewes were allocated into one of two groups, a Control group (n=10) that received no supplemental LH and a LH group (n=10) treated with 7.5mg of LH 24h after CIDR removal. Artificial inseminations (AI) were performed 42 and 48h after CIDR removal. The ovarian structures were evaluated by laparoscopy immediately before each AI and 5 days later (D21) when the embryos were collected. The LH ewes ovulated more frequently (P=0.05) before 42h than between 42 and 48h. Treatment with LH tended to increase the frequency of CL and to decrease the anovulatory follicles (P=0.08). The supplemental LH increased the frequency of ewes with a high SOV response (≥11 CL; P=0.05). In conclusion, supplemental LH increased the frequency of ewes with high SOV response and ovulating prior to 42h, however, there was no synchrony between ovulations. The supplemental LH also decreased the frequency of anovulatory follicles, although the ovulation rate and embryo yield were unaffected.Universidade Federal de Santa Maria2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021Ciência Rural v.42 n.6 2012reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782012000600021info:eu-repo/semantics/openAccessOliveira,Maria Emilia FrancoCordeiro,Mabel FreitasFerreira,Roberta MachadoSouza,Samuel FigueirêdoPieroni,Juliana Souza PintoRodrigues,Luiz Fernando de SouzaFonseca,Jeferson Ferreira daVicente,Wilter Ricardo Russianoeng2012-07-13T00:00:00ZRevista
dc.title.none.fl_str_mv Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
title Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
spellingShingle Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
Oliveira,Maria Emilia Franco
superovulation
LH
ovulation
embryo
sheep
title_short Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
title_full Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
title_fullStr Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
title_full_unstemmed Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
title_sort Does supplemental LH changes rate and time to ovulation and embryo yield in Santa Ines ewes treated for superovulation with FSH plus eCG?
author Oliveira,Maria Emilia Franco
author_facet Oliveira,Maria Emilia Franco
Cordeiro,Mabel Freitas
Ferreira,Roberta Machado
Souza,Samuel Figueirêdo
Pieroni,Juliana Souza Pinto
Rodrigues,Luiz Fernando de Souza
Fonseca,Jeferson Ferreira da
Vicente,Wilter Ricardo Russiano
author_role author
author2 Cordeiro,Mabel Freitas
Ferreira,Roberta Machado
Souza,Samuel Figueirêdo
Pieroni,Juliana Souza Pinto
Rodrigues,Luiz Fernando de Souza
Fonseca,Jeferson Ferreira da
Vicente,Wilter Ricardo Russiano
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Maria Emilia Franco
Cordeiro,Mabel Freitas
Ferreira,Roberta Machado
Souza,Samuel Figueirêdo
Pieroni,Juliana Souza Pinto
Rodrigues,Luiz Fernando de Souza
Fonseca,Jeferson Ferreira da
Vicente,Wilter Ricardo Russiano
dc.subject.por.fl_str_mv superovulation
LH
ovulation
embryo
sheep
topic superovulation
LH
ovulation
embryo
sheep
description The objective was to evaluate if supplemental LH given at the end of FSH treatment would synchronize the time of ovulation and increase the ovulation rate and embryo yield in Santa Ines ewes. Twenty superovulatory (SOV) programs were accomplished in cross-over design (60d interval). On D0, a CIDR device was inserted, and the device was replaced with a new one 7 days later, when 37.5µg of d-cloprostenol was administered. On D12, we started the SOV treatment, administering 256mg of pFSH 8 times, 12h apart. On D14, the CIDR was removed, and 200IU of eCG and 37.5µg of d-cloprostenol were administered. On D15, the ewes were allocated into one of two groups, a Control group (n=10) that received no supplemental LH and a LH group (n=10) treated with 7.5mg of LH 24h after CIDR removal. Artificial inseminations (AI) were performed 42 and 48h after CIDR removal. The ovarian structures were evaluated by laparoscopy immediately before each AI and 5 days later (D21) when the embryos were collected. The LH ewes ovulated more frequently (P=0.05) before 42h than between 42 and 48h. Treatment with LH tended to increase the frequency of CL and to decrease the anovulatory follicles (P=0.08). The supplemental LH increased the frequency of ewes with a high SOV response (≥11 CL; P=0.05). In conclusion, supplemental LH increased the frequency of ewes with high SOV response and ovulating prior to 42h, however, there was no synchrony between ovulations. The supplemental LH also decreased the frequency of anovulatory follicles, although the ovulation rate and embryo yield were unaffected.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782012000600021
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.42 n.6 2012
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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