Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods

Detalhes bibliográficos
Autor(a) principal: Mega,Nicolás Oliveira
Data de Publicação: 2011
Outros Autores: Revers,Luís Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000900012
Resumo: Here, it is presented a rapid and efficient method to obtain good quality DNA from small samples of arthropod tissues generating low quantities of hazardous wastes. This new method was compared with another homemade protocol using phenol and other two commercial kits. The quality of DNA obtained was checked by spectrophotometer and evaluated by an AFLP assay. Low shearing DNA was obtained from all samples and the best readings were observed to DNA recollected with the new method. The AFLP assay indicated that DNA obtained with all methods were suitable for use in molecular biology techniques sensitive to contaminants. However, homemade protocols were more efficient in recollect DNA than commercial kits, without lose any quality of samples. Also, they were less time and fund consuming, with costs ten times cheaper than commercial kits. The quicker, less pollutant and cheaper protocol was the one described here (USD 0.52 per sample).
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spelling Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropodsarthropod DNAhigh quality DNAmolecular biologyAFLPHere, it is presented a rapid and efficient method to obtain good quality DNA from small samples of arthropod tissues generating low quantities of hazardous wastes. This new method was compared with another homemade protocol using phenol and other two commercial kits. The quality of DNA obtained was checked by spectrophotometer and evaluated by an AFLP assay. Low shearing DNA was obtained from all samples and the best readings were observed to DNA recollected with the new method. The AFLP assay indicated that DNA obtained with all methods were suitable for use in molecular biology techniques sensitive to contaminants. However, homemade protocols were more efficient in recollect DNA than commercial kits, without lose any quality of samples. Also, they were less time and fund consuming, with costs ten times cheaper than commercial kits. The quicker, less pollutant and cheaper protocol was the one described here (USD 0.52 per sample).Universidade Federal de Santa Maria2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000900012Ciência Rural v.41 n.9 2011reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782011005000112info:eu-repo/semantics/openAccessMega,Nicolás OliveiraRevers,Luís Fernandoeng2011-11-11T00:00:00ZRevista
dc.title.none.fl_str_mv Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
title Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
spellingShingle Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
Mega,Nicolás Oliveira
arthropod DNA
high quality DNA
molecular biology
AFLP
title_short Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
title_full Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
title_fullStr Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
title_full_unstemmed Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
title_sort Developing a rapid, efficient and low cost method for rapid DNA extraction from arthropods
author Mega,Nicolás Oliveira
author_facet Mega,Nicolás Oliveira
Revers,Luís Fernando
author_role author
author2 Revers,Luís Fernando
author2_role author
dc.contributor.author.fl_str_mv Mega,Nicolás Oliveira
Revers,Luís Fernando
dc.subject.por.fl_str_mv arthropod DNA
high quality DNA
molecular biology
AFLP
topic arthropod DNA
high quality DNA
molecular biology
AFLP
description Here, it is presented a rapid and efficient method to obtain good quality DNA from small samples of arthropod tissues generating low quantities of hazardous wastes. This new method was compared with another homemade protocol using phenol and other two commercial kits. The quality of DNA obtained was checked by spectrophotometer and evaluated by an AFLP assay. Low shearing DNA was obtained from all samples and the best readings were observed to DNA recollected with the new method. The AFLP assay indicated that DNA obtained with all methods were suitable for use in molecular biology techniques sensitive to contaminants. However, homemade protocols were more efficient in recollect DNA than commercial kits, without lose any quality of samples. Also, they were less time and fund consuming, with costs ten times cheaper than commercial kits. The quicker, less pollutant and cheaper protocol was the one described here (USD 0.52 per sample).
publishDate 2011
dc.date.none.fl_str_mv 2011-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000900012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000900012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782011005000112
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.41 n.9 2011
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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