Experimental infection of horses with Vaccinia virus

Detalhes bibliográficos
Autor(a) principal: Barbosa,Claudio Henrique Gonçalves
Data de Publicação: 2016
Outros Autores: Sant'Ana,Fabiano José Ferreira de, Cargnelutti,Juliana Felipetto, Flores,Eduardo Furtado, Teixeira Neto,Antônio Raphael, Santana,Rosália Bezerra de, Reis Junior,Janildo Ludolf
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300519
Resumo: ABSTRACT: Vaccinia virus (VACV) is the etiologic agent of bovine vaccinia, an emerging zoonotic disease with potential health issues for dairy herds and humans. VACV may occasionally infect other species, including horses. In this sense, an outbreak of VACV disease in horses was described in Pelotas, RS, in 2008, where a co-infection with two VACV strains (named Pelotas Virus 1 [P1V] and Pelotas Virus 2 [P2V]) was detected. Considering the rare occurrence of VACV infection in horses, the objective of this study was to investigate the susceptibility and pathogenesis of VACV infection in this species. Six adult horses were inoculated with VACV P1V or P2V (106.3TCID50/ml) through scarification of the nasolabial surface and monitored for virological and clinical aspects during 28 days. Four inoculated horses (4/6) developed mild lesions in the site of inoculation. Ulcers and scabs restricted to inoculated areas were observed between days 2 and 8 post-inoculation (pi). Microscopically there were acanthosis, ballooning degeneration of the stratum spinosum, necrosis and loss of the epidermis. Infiltration of neutrophils, macrophages and lymphocytes were observed in the dermis. Intracytoplasmic eosinophilic inclusions were infrequently observed in degenerate keratinocytes from adjacent necrotic areas. Virus shedding was detected between days 4 and 8 pi by PCR and virus isolation (infectious virus) from the lesions of one horse inoculated with P2V. No neutralizing antibodies were detected in inoculated animals at day 28 pi. In summary, inoculation of horses with VACV P1V and P2V isolates resulted in a low level of replication and at low frequency, with mild cutaneous lesions, when compared with the course of infection of other susceptible species to VACV. Therefore, horses possibly have a low potential for viral maintenance and transmission to other species, albeit being susceptible to VACV infection.
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spelling Experimental infection of horses with Vaccinia virusVACVexperimental infectionhorsesbovine vacciniaABSTRACT: Vaccinia virus (VACV) is the etiologic agent of bovine vaccinia, an emerging zoonotic disease with potential health issues for dairy herds and humans. VACV may occasionally infect other species, including horses. In this sense, an outbreak of VACV disease in horses was described in Pelotas, RS, in 2008, where a co-infection with two VACV strains (named Pelotas Virus 1 [P1V] and Pelotas Virus 2 [P2V]) was detected. Considering the rare occurrence of VACV infection in horses, the objective of this study was to investigate the susceptibility and pathogenesis of VACV infection in this species. Six adult horses were inoculated with VACV P1V or P2V (106.3TCID50/ml) through scarification of the nasolabial surface and monitored for virological and clinical aspects during 28 days. Four inoculated horses (4/6) developed mild lesions in the site of inoculation. Ulcers and scabs restricted to inoculated areas were observed between days 2 and 8 post-inoculation (pi). Microscopically there were acanthosis, ballooning degeneration of the stratum spinosum, necrosis and loss of the epidermis. Infiltration of neutrophils, macrophages and lymphocytes were observed in the dermis. Intracytoplasmic eosinophilic inclusions were infrequently observed in degenerate keratinocytes from adjacent necrotic areas. Virus shedding was detected between days 4 and 8 pi by PCR and virus isolation (infectious virus) from the lesions of one horse inoculated with P2V. No neutralizing antibodies were detected in inoculated animals at day 28 pi. In summary, inoculation of horses with VACV P1V and P2V isolates resulted in a low level of replication and at low frequency, with mild cutaneous lesions, when compared with the course of infection of other susceptible species to VACV. Therefore, horses possibly have a low potential for viral maintenance and transmission to other species, albeit being susceptible to VACV infection.Universidade Federal de Santa Maria2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300519Ciência Rural v.46 n.3 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20150289info:eu-repo/semantics/openAccessBarbosa,Claudio Henrique GonçalvesSant'Ana,Fabiano José Ferreira deCargnelutti,Juliana FelipettoFlores,Eduardo FurtadoTeixeira Neto,Antônio RaphaelSantana,Rosália Bezerra deReis Junior,Janildo Ludolfeng2016-04-04T00:00:00ZRevista
dc.title.none.fl_str_mv Experimental infection of horses with Vaccinia virus
title Experimental infection of horses with Vaccinia virus
spellingShingle Experimental infection of horses with Vaccinia virus
Barbosa,Claudio Henrique Gonçalves
VACV
experimental infection
horses
bovine vaccinia
title_short Experimental infection of horses with Vaccinia virus
title_full Experimental infection of horses with Vaccinia virus
title_fullStr Experimental infection of horses with Vaccinia virus
title_full_unstemmed Experimental infection of horses with Vaccinia virus
title_sort Experimental infection of horses with Vaccinia virus
author Barbosa,Claudio Henrique Gonçalves
author_facet Barbosa,Claudio Henrique Gonçalves
Sant'Ana,Fabiano José Ferreira de
Cargnelutti,Juliana Felipetto
Flores,Eduardo Furtado
Teixeira Neto,Antônio Raphael
Santana,Rosália Bezerra de
Reis Junior,Janildo Ludolf
author_role author
author2 Sant'Ana,Fabiano José Ferreira de
Cargnelutti,Juliana Felipetto
Flores,Eduardo Furtado
Teixeira Neto,Antônio Raphael
Santana,Rosália Bezerra de
Reis Junior,Janildo Ludolf
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa,Claudio Henrique Gonçalves
Sant'Ana,Fabiano José Ferreira de
Cargnelutti,Juliana Felipetto
Flores,Eduardo Furtado
Teixeira Neto,Antônio Raphael
Santana,Rosália Bezerra de
Reis Junior,Janildo Ludolf
dc.subject.por.fl_str_mv VACV
experimental infection
horses
bovine vaccinia
topic VACV
experimental infection
horses
bovine vaccinia
description ABSTRACT: Vaccinia virus (VACV) is the etiologic agent of bovine vaccinia, an emerging zoonotic disease with potential health issues for dairy herds and humans. VACV may occasionally infect other species, including horses. In this sense, an outbreak of VACV disease in horses was described in Pelotas, RS, in 2008, where a co-infection with two VACV strains (named Pelotas Virus 1 [P1V] and Pelotas Virus 2 [P2V]) was detected. Considering the rare occurrence of VACV infection in horses, the objective of this study was to investigate the susceptibility and pathogenesis of VACV infection in this species. Six adult horses were inoculated with VACV P1V or P2V (106.3TCID50/ml) through scarification of the nasolabial surface and monitored for virological and clinical aspects during 28 days. Four inoculated horses (4/6) developed mild lesions in the site of inoculation. Ulcers and scabs restricted to inoculated areas were observed between days 2 and 8 post-inoculation (pi). Microscopically there were acanthosis, ballooning degeneration of the stratum spinosum, necrosis and loss of the epidermis. Infiltration of neutrophils, macrophages and lymphocytes were observed in the dermis. Intracytoplasmic eosinophilic inclusions were infrequently observed in degenerate keratinocytes from adjacent necrotic areas. Virus shedding was detected between days 4 and 8 pi by PCR and virus isolation (infectious virus) from the lesions of one horse inoculated with P2V. No neutralizing antibodies were detected in inoculated animals at day 28 pi. In summary, inoculation of horses with VACV P1V and P2V isolates resulted in a low level of replication and at low frequency, with mild cutaneous lesions, when compared with the course of infection of other susceptible species to VACV. Therefore, horses possibly have a low potential for viral maintenance and transmission to other species, albeit being susceptible to VACV infection.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300519
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000300519
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20150289
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.46 n.3 2016
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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