ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT
Autor(a) principal: | |
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Data de Publicação: | 1994 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300020 |
Resumo: | Six calves were immunized with schistosomula of Schistosoma bovis irradiated at 3 or 20 Kilorad (Krad) and three calves were kept as controls. Twenty four weeks post immunization, three calves (one from the 20 Krad and two from the 3 Krad group) were challenged with normal cercaria of S. bovis. The immune response was monitored by agar gel immunodiffusion (AGID) and the Enzyme Linked Immunosorbent Assay (ELISA) using adult worm antigen. Using AGID, precipitin lines were observed only with sera from challenged animals. Using ELISA, the immune response of the vaccinated calves was first detected by 2-3 weeks, peaking by 6-8 weeks post vaccination. The immune response of the three challenged calves was elevated by 2 weeks post challenge, peaking at 8-10 weeks post challenge and remained high throughout the experimental period. This study suggests that ELISA could be used for diagnosis of bovine schistosomiasis. |
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Ciência rural (Online) |
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ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORTELISASchistosoma bovisvaccineSix calves were immunized with schistosomula of Schistosoma bovis irradiated at 3 or 20 Kilorad (Krad) and three calves were kept as controls. Twenty four weeks post immunization, three calves (one from the 20 Krad and two from the 3 Krad group) were challenged with normal cercaria of S. bovis. The immune response was monitored by agar gel immunodiffusion (AGID) and the Enzyme Linked Immunosorbent Assay (ELISA) using adult worm antigen. Using AGID, precipitin lines were observed only with sera from challenged animals. Using ELISA, the immune response of the vaccinated calves was first detected by 2-3 weeks, peaking by 6-8 weeks post vaccination. The immune response of the three challenged calves was elevated by 2 weeks post challenge, peaking at 8-10 weeks post challenge and remained high throughout the experimental period. This study suggests that ELISA could be used for diagnosis of bovine schistosomiasis.Universidade Federal de Santa Maria1994-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300020Ciência Rural v.24 n.3 1994reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84781994000300020info:eu-repo/semantics/openAccessAradaib,Imadeldin ElaminAbbas,BabikerOsburn,BennieBushara,Hamid OmerTaylor,Martineng2014-08-25T00:00:00ZRevista |
dc.title.none.fl_str_mv |
ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT |
title |
ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT |
spellingShingle |
ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT Aradaib,Imadeldin Elamin ELISA Schistosoma bovis vaccine |
title_short |
ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT |
title_full |
ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT |
title_fullStr |
ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT |
title_full_unstemmed |
ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT |
title_sort |
ELISA FOR BOVINE SCHISTOSOMIASIS VACCINE EVALUATION: A PRELIMINARY REPORT |
author |
Aradaib,Imadeldin Elamin |
author_facet |
Aradaib,Imadeldin Elamin Abbas,Babiker Osburn,Bennie Bushara,Hamid Omer Taylor,Martin |
author_role |
author |
author2 |
Abbas,Babiker Osburn,Bennie Bushara,Hamid Omer Taylor,Martin |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Aradaib,Imadeldin Elamin Abbas,Babiker Osburn,Bennie Bushara,Hamid Omer Taylor,Martin |
dc.subject.por.fl_str_mv |
ELISA Schistosoma bovis vaccine |
topic |
ELISA Schistosoma bovis vaccine |
description |
Six calves were immunized with schistosomula of Schistosoma bovis irradiated at 3 or 20 Kilorad (Krad) and three calves were kept as controls. Twenty four weeks post immunization, three calves (one from the 20 Krad and two from the 3 Krad group) were challenged with normal cercaria of S. bovis. The immune response was monitored by agar gel immunodiffusion (AGID) and the Enzyme Linked Immunosorbent Assay (ELISA) using adult worm antigen. Using AGID, precipitin lines were observed only with sera from challenged animals. Using ELISA, the immune response of the vaccinated calves was first detected by 2-3 weeks, peaking by 6-8 weeks post vaccination. The immune response of the three challenged calves was elevated by 2 weeks post challenge, peaking at 8-10 weeks post challenge and remained high throughout the experimental period. This study suggests that ELISA could be used for diagnosis of bovine schistosomiasis. |
publishDate |
1994 |
dc.date.none.fl_str_mv |
1994-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300020 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781994000300020 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84781994000300020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.24 n.3 1994 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140518709755904 |