Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200310 |
Resumo: | ABSTRACT: The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone marrow samples were efficient for the molecular diagnosis of canine ehrlichiosis, particularly when there was a negative blood sample, although infection was present. |
id |
UFSM-2_6647422cf10b5c2615aa5d806b1a0880 |
---|---|
oai_identifier_str |
oai:scielo:S0103-84782016000200310 |
network_acronym_str |
UFSM-2 |
network_name_str |
Ciência rural (Online) |
repository_id_str |
|
spelling |
Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samplescanine ehrlichiosisdiagnosisnested PCRbiological samples.ABSTRACT: The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone marrow samples were efficient for the molecular diagnosis of canine ehrlichiosis, particularly when there was a negative blood sample, although infection was present.Universidade Federal de Santa Maria2016-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200310Ciência Rural v.46 n.2 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20150090info:eu-repo/semantics/openAccessMakino,HericaSousa,Valéria Régia FrancoFujimori,MahyumiRodrigues,Juliana YukiDias,Alvaro Felipe Lima RuyDutra,ValériaNakazato,LucianoAlmeida,Arleana do Bom Parto Ferreira deeng2016-02-26T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples |
title |
Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples |
spellingShingle |
Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples Makino,Herica canine ehrlichiosis diagnosis nested PCR biological samples. |
title_short |
Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples |
title_full |
Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples |
title_fullStr |
Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples |
title_full_unstemmed |
Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples |
title_sort |
Ehrlichia canis detection in dogs from Várzea Grande: a comparative analysis of blood and bone marrow samples |
author |
Makino,Herica |
author_facet |
Makino,Herica Sousa,Valéria Régia Franco Fujimori,Mahyumi Rodrigues,Juliana Yuki Dias,Alvaro Felipe Lima Ruy Dutra,Valéria Nakazato,Luciano Almeida,Arleana do Bom Parto Ferreira de |
author_role |
author |
author2 |
Sousa,Valéria Régia Franco Fujimori,Mahyumi Rodrigues,Juliana Yuki Dias,Alvaro Felipe Lima Ruy Dutra,Valéria Nakazato,Luciano Almeida,Arleana do Bom Parto Ferreira de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Makino,Herica Sousa,Valéria Régia Franco Fujimori,Mahyumi Rodrigues,Juliana Yuki Dias,Alvaro Felipe Lima Ruy Dutra,Valéria Nakazato,Luciano Almeida,Arleana do Bom Parto Ferreira de |
dc.subject.por.fl_str_mv |
canine ehrlichiosis diagnosis nested PCR biological samples. |
topic |
canine ehrlichiosis diagnosis nested PCR biological samples. |
description |
ABSTRACT: The objective of this study was to compare the DNA detection of Ehrlichia canis in blood and bone marrow to determine the prevalence of the agent in Várzea Grande, Mato Grosso. Blood samples and bone marrow from 80 dogs of both sexes, different breeds and age, were collected and processed for a cross-sectional study performed using nested PCR. Of the 80 dogs, 61 (76.3%) had E. canis DNA in one of the samples. The buffy coat was positive in 42 dogs (52.5%) and the bone marrow was positive in 33 (41.3%). There was no significant association between the positive biological samples of either the buffy coat or bone marrow and the presence or absence of clinical signs (P=0.49). No risk factor was associated with infection in the studied area. The bone marrow samples were efficient for the molecular diagnosis of canine ehrlichiosis, particularly when there was a negative blood sample, although infection was present. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200310 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200310 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20150090 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.46 n.2 2016 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140548631920640 |