INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000100016 |
Resumo: | To evaluate the effect of follicular fluid on in vitro maturation, pig oocytes were cultured in the presence of hormones where 10% of fetal calf serum (FCS), 10% of follicular fluid from large follicles (l-pFF), 10% of follicular fluid from medium follicles (m-pFF) or no supplement were added. When oocytes where matured in medium containing the hormones the addition of different supplements did not affect (P<0.05) nuclear maturation. However, changing the supplement altered the cytoplasmic maturation, with higher rate (P<0.05) observed in the l-pFF group. To determine the effect of the presence of hormone and/or supplement during maturation, the oocytes were cultured either in presence of TCM-199 alone, with hormones, with 10% l-pFF or with hormones and 10% l-pFF. The highest proportion of oocytes undergoing nuclear and cytoplasmic maturation was obtained when both hormones and follicular fluid were present. Cumulus expansion had a significant (P<0.05) effect on cytoplasmic maturation with the non-expanded groups showing a lower percentage of maturation in all groups. When the adequacy of gonadotropins levels were evaluated by adding higher or lower concentrations into the maturation medium neither beneficial nor detrimental effects were observed in either nuclear or cytoplasmic maturation. These results suggest that changes in the composition of the medium can alter the percentage of oocytes completing maturation. Follicular fluid combined with hormones was found to give better conditions for pig oocyte maturation in vitro. |
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INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTESfollicular fluidoocyte maturationgonadotrophinsporcineTo evaluate the effect of follicular fluid on in vitro maturation, pig oocytes were cultured in the presence of hormones where 10% of fetal calf serum (FCS), 10% of follicular fluid from large follicles (l-pFF), 10% of follicular fluid from medium follicles (m-pFF) or no supplement were added. When oocytes where matured in medium containing the hormones the addition of different supplements did not affect (P<0.05) nuclear maturation. However, changing the supplement altered the cytoplasmic maturation, with higher rate (P<0.05) observed in the l-pFF group. To determine the effect of the presence of hormone and/or supplement during maturation, the oocytes were cultured either in presence of TCM-199 alone, with hormones, with 10% l-pFF or with hormones and 10% l-pFF. The highest proportion of oocytes undergoing nuclear and cytoplasmic maturation was obtained when both hormones and follicular fluid were present. Cumulus expansion had a significant (P<0.05) effect on cytoplasmic maturation with the non-expanded groups showing a lower percentage of maturation in all groups. When the adequacy of gonadotropins levels were evaluated by adding higher or lower concentrations into the maturation medium neither beneficial nor detrimental effects were observed in either nuclear or cytoplasmic maturation. These results suggest that changes in the composition of the medium can alter the percentage of oocytes completing maturation. Follicular fluid combined with hormones was found to give better conditions for pig oocyte maturation in vitro.Universidade Federal de Santa Maria2001-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000100016Ciência Rural v.31 n.1 2001reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782001000100016info:eu-repo/semantics/openAccessDode,Margot Alves NunesGraves,Charleseng2005-06-10T00:00:00ZRevista |
dc.title.none.fl_str_mv |
INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES |
title |
INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES |
spellingShingle |
INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES Dode,Margot Alves Nunes follicular fluid oocyte maturation gonadotrophins porcine |
title_short |
INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES |
title_full |
INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES |
title_fullStr |
INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES |
title_full_unstemmed |
INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES |
title_sort |
INFLUENCE OF HORMONES AND FOLLICULAR FLUID ON MATURATION OF PIG OOCYTES |
author |
Dode,Margot Alves Nunes |
author_facet |
Dode,Margot Alves Nunes Graves,Charles |
author_role |
author |
author2 |
Graves,Charles |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Dode,Margot Alves Nunes Graves,Charles |
dc.subject.por.fl_str_mv |
follicular fluid oocyte maturation gonadotrophins porcine |
topic |
follicular fluid oocyte maturation gonadotrophins porcine |
description |
To evaluate the effect of follicular fluid on in vitro maturation, pig oocytes were cultured in the presence of hormones where 10% of fetal calf serum (FCS), 10% of follicular fluid from large follicles (l-pFF), 10% of follicular fluid from medium follicles (m-pFF) or no supplement were added. When oocytes where matured in medium containing the hormones the addition of different supplements did not affect (P<0.05) nuclear maturation. However, changing the supplement altered the cytoplasmic maturation, with higher rate (P<0.05) observed in the l-pFF group. To determine the effect of the presence of hormone and/or supplement during maturation, the oocytes were cultured either in presence of TCM-199 alone, with hormones, with 10% l-pFF or with hormones and 10% l-pFF. The highest proportion of oocytes undergoing nuclear and cytoplasmic maturation was obtained when both hormones and follicular fluid were present. Cumulus expansion had a significant (P<0.05) effect on cytoplasmic maturation with the non-expanded groups showing a lower percentage of maturation in all groups. When the adequacy of gonadotropins levels were evaluated by adding higher or lower concentrations into the maturation medium neither beneficial nor detrimental effects were observed in either nuclear or cytoplasmic maturation. These results suggest that changes in the composition of the medium can alter the percentage of oocytes completing maturation. Follicular fluid combined with hormones was found to give better conditions for pig oocyte maturation in vitro. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000100016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782001000100016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782001000100016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.31 n.1 2001 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140522029547520 |