Feline herpesvirus 1 viral load related to environmental factors in sheltered cats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454 |
Resumo: | ABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection. Different routines regarding new arrivals were directly related to FHV-1 viral load. Shelters where new arrivals occurred on daily basis had the highest viral load (2.69x108 copies/µL), while those shelters where new arrivals had not occurred in the few months prior to the beginning of the study had the lowest rate (1.63x103 copies/µL). Environmental factors directly influenced FHV-1 DNA viral load. This study highlighted the need to improve the management approach in the animal shelter environment to reduce stressful situations responsible for FHV-1 reactivation and higher viral load quantification. |
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Feline herpesvirus 1 viral load related to environmental factors in sheltered catsFHV-1conjunctivitisshelter medicineqPCRanimal shelterABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection. Different routines regarding new arrivals were directly related to FHV-1 viral load. Shelters where new arrivals occurred on daily basis had the highest viral load (2.69x108 copies/µL), while those shelters where new arrivals had not occurred in the few months prior to the beginning of the study had the lowest rate (1.63x103 copies/µL). Environmental factors directly influenced FHV-1 DNA viral load. This study highlighted the need to improve the management approach in the animal shelter environment to reduce stressful situations responsible for FHV-1 reactivation and higher viral load quantification.Universidade Federal de Santa Maria2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454Ciência Rural v.49 n.5 2019reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20190067info:eu-repo/semantics/openAccessBaumworcel,NatashaPereira,Joylson de JesusSoares,Ana Maria BarrosSouza,Guilherme NunesAlmosny,Nadia Regina PereiraCastro,Tatiana Xavier deeng2019-06-10T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
title |
Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
spellingShingle |
Feline herpesvirus 1 viral load related to environmental factors in sheltered cats Baumworcel,Natasha FHV-1 conjunctivitis shelter medicine qPCR animal shelter |
title_short |
Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
title_full |
Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
title_fullStr |
Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
title_full_unstemmed |
Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
title_sort |
Feline herpesvirus 1 viral load related to environmental factors in sheltered cats |
author |
Baumworcel,Natasha |
author_facet |
Baumworcel,Natasha Pereira,Joylson de Jesus Soares,Ana Maria Barros Souza,Guilherme Nunes Almosny,Nadia Regina Pereira Castro,Tatiana Xavier de |
author_role |
author |
author2 |
Pereira,Joylson de Jesus Soares,Ana Maria Barros Souza,Guilherme Nunes Almosny,Nadia Regina Pereira Castro,Tatiana Xavier de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Baumworcel,Natasha Pereira,Joylson de Jesus Soares,Ana Maria Barros Souza,Guilherme Nunes Almosny,Nadia Regina Pereira Castro,Tatiana Xavier de |
dc.subject.por.fl_str_mv |
FHV-1 conjunctivitis shelter medicine qPCR animal shelter |
topic |
FHV-1 conjunctivitis shelter medicine qPCR animal shelter |
description |
ABSTRACT: Shelter environment stress factors are related to FHV-1 viral reactivation. However, comparisons between conjunctival viral load and environmental factors have not been commonly evaluated. The aim of this study was to correlate FHV-1 viral load in domestic cats with and without clinical signs of conjunctivitis to shelter design in order to use FHV-1 viral load as a parameter of “health management”. Cats from four different shelters underwent an ophthalmological examination. Samples were collected by rolling a DNA/RNAse-free cytobrush over the ventral conjunctival fornix and were stored in 1.5 mL sterile microtubes in 500 μL of Eagle’s minimum essential medium and kept at 4 ºC. Molecular procedures were performed up to 48 hours after collection. Different routines regarding new arrivals were directly related to FHV-1 viral load. Shelters where new arrivals occurred on daily basis had the highest viral load (2.69x108 copies/µL), while those shelters where new arrivals had not occurred in the few months prior to the beginning of the study had the lowest rate (1.63x103 copies/µL). Environmental factors directly influenced FHV-1 DNA viral load. This study highlighted the need to improve the management approach in the animal shelter environment to reduce stressful situations responsible for FHV-1 reactivation and higher viral load quantification. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500454 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20190067 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.49 n.5 2019 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140553551839232 |