Genetic divergence among Dimorphandra spp. accessions using RAPD markers
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400010 |
Resumo: | The genus Dimorphandra has distinguish relevance considering either medicinal or biodiversity aspects because it includes two species that are economically important flavonoids sources for pharmachemical industry (D. mollis Benth. and D. gardneriana Tul.), and species endemic to Brazil, such as D. jorgei Silva and D. wilsonii Rizz., threatened by extinction. In order to evaluate variability among accessions of D. mollis (fava-d'anta), D. gardneriana and D. wilsonii, it was collected fruit from individual plants from three Brazilian states in a total of 57 accessions, which were analyzed with RAPD markers. It was used 20 seeds per progeny; the DNA was extracted from fully-formed young leaves, which were collected in bulk. The data were analyzed using a binary matrix, in which the score one represented presence of a band and zero, absence. The similarity matrix was developed by using the arithmetic complement of the Jaccard index, later grouped based on the Neighbor Joining algorithm. It was found considerable intra and inter specific variability in Dimorphandra spp., which were separated into four groups. Though genetic variability was found, the collecting trips showed that most of these areas are subject to loss of genetic resources of fava-d'anta due to the following factors: continuous anthropic activity, propensity for natural fires, and loss of natural seed dispersers (large fruit-eating mammals). Therefore, protections of these areas and ex situ conservation are essentials for the maintenance of genetic variability of these species. |
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Genetic divergence among Dimorphandra spp. accessions using RAPD markersfava-d'antmolecular characterizationplant genetic resourcegermoplasmThe genus Dimorphandra has distinguish relevance considering either medicinal or biodiversity aspects because it includes two species that are economically important flavonoids sources for pharmachemical industry (D. mollis Benth. and D. gardneriana Tul.), and species endemic to Brazil, such as D. jorgei Silva and D. wilsonii Rizz., threatened by extinction. In order to evaluate variability among accessions of D. mollis (fava-d'anta), D. gardneriana and D. wilsonii, it was collected fruit from individual plants from three Brazilian states in a total of 57 accessions, which were analyzed with RAPD markers. It was used 20 seeds per progeny; the DNA was extracted from fully-formed young leaves, which were collected in bulk. The data were analyzed using a binary matrix, in which the score one represented presence of a band and zero, absence. The similarity matrix was developed by using the arithmetic complement of the Jaccard index, later grouped based on the Neighbor Joining algorithm. It was found considerable intra and inter specific variability in Dimorphandra spp., which were separated into four groups. Though genetic variability was found, the collecting trips showed that most of these areas are subject to loss of genetic resources of fava-d'anta due to the following factors: continuous anthropic activity, propensity for natural fires, and loss of natural seed dispersers (large fruit-eating mammals). Therefore, protections of these areas and ex situ conservation are essentials for the maintenance of genetic variability of these species.Universidade Federal de Santa Maria2011-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400010Ciência Rural v.41 n.4 2011reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782011005000024info:eu-repo/semantics/openAccessSudré,Cláudia PomboRodrigues,RosanaGonçalves,Leandro Simões AzeredoMartins,Ernane RoniePereira,Messias GonzagaSantos,Marilene Hilma doseng2011-10-13T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Genetic divergence among Dimorphandra spp. accessions using RAPD markers |
title |
Genetic divergence among Dimorphandra spp. accessions using RAPD markers |
spellingShingle |
Genetic divergence among Dimorphandra spp. accessions using RAPD markers Sudré,Cláudia Pombo fava-d'ant molecular characterization plant genetic resource germoplasm |
title_short |
Genetic divergence among Dimorphandra spp. accessions using RAPD markers |
title_full |
Genetic divergence among Dimorphandra spp. accessions using RAPD markers |
title_fullStr |
Genetic divergence among Dimorphandra spp. accessions using RAPD markers |
title_full_unstemmed |
Genetic divergence among Dimorphandra spp. accessions using RAPD markers |
title_sort |
Genetic divergence among Dimorphandra spp. accessions using RAPD markers |
author |
Sudré,Cláudia Pombo |
author_facet |
Sudré,Cláudia Pombo Rodrigues,Rosana Gonçalves,Leandro Simões Azeredo Martins,Ernane Ronie Pereira,Messias Gonzaga Santos,Marilene Hilma dos |
author_role |
author |
author2 |
Rodrigues,Rosana Gonçalves,Leandro Simões Azeredo Martins,Ernane Ronie Pereira,Messias Gonzaga Santos,Marilene Hilma dos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Sudré,Cláudia Pombo Rodrigues,Rosana Gonçalves,Leandro Simões Azeredo Martins,Ernane Ronie Pereira,Messias Gonzaga Santos,Marilene Hilma dos |
dc.subject.por.fl_str_mv |
fava-d'ant molecular characterization plant genetic resource germoplasm |
topic |
fava-d'ant molecular characterization plant genetic resource germoplasm |
description |
The genus Dimorphandra has distinguish relevance considering either medicinal or biodiversity aspects because it includes two species that are economically important flavonoids sources for pharmachemical industry (D. mollis Benth. and D. gardneriana Tul.), and species endemic to Brazil, such as D. jorgei Silva and D. wilsonii Rizz., threatened by extinction. In order to evaluate variability among accessions of D. mollis (fava-d'anta), D. gardneriana and D. wilsonii, it was collected fruit from individual plants from three Brazilian states in a total of 57 accessions, which were analyzed with RAPD markers. It was used 20 seeds per progeny; the DNA was extracted from fully-formed young leaves, which were collected in bulk. The data were analyzed using a binary matrix, in which the score one represented presence of a band and zero, absence. The similarity matrix was developed by using the arithmetic complement of the Jaccard index, later grouped based on the Neighbor Joining algorithm. It was found considerable intra and inter specific variability in Dimorphandra spp., which were separated into four groups. Though genetic variability was found, the collecting trips showed that most of these areas are subject to loss of genetic resources of fava-d'anta due to the following factors: continuous anthropic activity, propensity for natural fires, and loss of natural seed dispersers (large fruit-eating mammals). Therefore, protections of these areas and ex situ conservation are essentials for the maintenance of genetic variability of these species. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782011005000024 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.41 n.4 2011 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140539723218944 |