Morphological, biochemical, and molecular characterization of Meloidogyne spp. populations from Brazilian soybean production regions

• Autor(a) principal: Oliveira,Camilla Martins de
• Data de Publicação: 2017
• Outros Autores: Souza Junior,Ismail Teodoro, Araújo Filho,Jerônimo Vieira de, Freitas,Marcos Augusto de, Rocha,Mara Rúbia da, Petrofeza,Silvana
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Ciência Rural
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000500151
• topic: root-knot nematode; Glycine max; esterase; molecular identification

ABSTRACT: Soybean is a commodity of great economic importance worldwide, particularly in Brazil, world’s second largest producer. Nematodes, especially those of the Meloidogyne genus, severely limit productivity. Identification of nematode species is important for effective soybean management. Here, 26 populations of root-knot nematode (Meloidogyne spp.) from 15 municipalities in the states of Bahia, Mato Grosso, Goias, and Minas Gerais were characterized based on the morphology of the female perineal region, esterase profile, and identification based on amplification of specific regions of the population genome. Among the Meloidogyne spp. populations obtained, M. incognita and M. javanica, were identified. No mixed populations were present in the samples. Diagnosis based on molecular analysis was shown to be reliable and the fastest for characterization of nematode populations compared to other methods analyzed.