Selenium supplementation prevents DNA damage in ram spermatozoa

Detalhes bibliográficos
Autor(a) principal: Moya,Carla Fredrichsen
Data de Publicação: 2021
Outros Autores: Piagentini,Marcelo, Silva,Danilo da Cunha, Fernandes,Fábio Henrique, Salvadori,Daisy Maria Fávero, Oba,Eunice
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000100701
Resumo: ABSTRACT: In the present study, we aimed to evaluate the effects of different concentrations of selenium (Se) ovine nutritional supplementation on spermatozoa DNA integrity. Thirty male ovines (age: 10 months) were used. They were fed with hay and ram food in an intensive system, which was divided into stalls (5 m long and 3 m wide) with feeding troughs, and had ad libitum access to food and water. Ovines in group 1 (G1, the negative control) received mineral salt supplementation without Se; ovines in G2 received the same mineral salt mixed with 5 mg Se (as sodium selenite)/kg mineral supplement;ovines in G3 received 10 mg Se/kg mineral supplement; ovines in G4 received 15 mg Se/kg mineral supplement; and ovines in G5 received 20 mg Se/kg mineral supplement. Ovines in all groups remained untreated for 14 days, followed by a treatment period of 56 days. Semen samples were obtained by electroejaculation. The DNA damage in semen samples was evaluated using the comet assay. The experimental design was implemented using a 5 × 5 Latin Square, i.e., five treatments and five experimental periods. The mean differences were compared using Tukey’s test at a significance level of 5%. The control group (G1) showed a high percentage of DNA damage compared to the Se-treated groups (G2-G5). Therefore, Se supplementation could decrease the basal level of DNA damage in sperm cells, suggesting that Se might exert protective effects on sperm DNA.
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spelling Selenium supplementation prevents DNA damage in ram spermatozoachemopreventioncomet assaysemenovine.ABSTRACT: In the present study, we aimed to evaluate the effects of different concentrations of selenium (Se) ovine nutritional supplementation on spermatozoa DNA integrity. Thirty male ovines (age: 10 months) were used. They were fed with hay and ram food in an intensive system, which was divided into stalls (5 m long and 3 m wide) with feeding troughs, and had ad libitum access to food and water. Ovines in group 1 (G1, the negative control) received mineral salt supplementation without Se; ovines in G2 received the same mineral salt mixed with 5 mg Se (as sodium selenite)/kg mineral supplement;ovines in G3 received 10 mg Se/kg mineral supplement; ovines in G4 received 15 mg Se/kg mineral supplement; and ovines in G5 received 20 mg Se/kg mineral supplement. Ovines in all groups remained untreated for 14 days, followed by a treatment period of 56 days. Semen samples were obtained by electroejaculation. The DNA damage in semen samples was evaluated using the comet assay. The experimental design was implemented using a 5 × 5 Latin Square, i.e., five treatments and five experimental periods. The mean differences were compared using Tukey’s test at a significance level of 5%. The control group (G1) showed a high percentage of DNA damage compared to the Se-treated groups (G2-G5). Therefore, Se supplementation could decrease the basal level of DNA damage in sperm cells, suggesting that Se might exert protective effects on sperm DNA.Universidade Federal de Santa Maria2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000100701Ciência Rural v.51 n.1 2021reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20200102info:eu-repo/semantics/openAccessMoya,Carla FredrichsenPiagentini,MarceloSilva,Danilo da CunhaFernandes,Fábio HenriqueSalvadori,Daisy Maria FáveroOba,Euniceeng2020-12-07T00:00:00ZRevista
dc.title.none.fl_str_mv Selenium supplementation prevents DNA damage in ram spermatozoa
title Selenium supplementation prevents DNA damage in ram spermatozoa
spellingShingle Selenium supplementation prevents DNA damage in ram spermatozoa
Moya,Carla Fredrichsen
chemoprevention
comet assay
semen
ovine.
title_short Selenium supplementation prevents DNA damage in ram spermatozoa
title_full Selenium supplementation prevents DNA damage in ram spermatozoa
title_fullStr Selenium supplementation prevents DNA damage in ram spermatozoa
title_full_unstemmed Selenium supplementation prevents DNA damage in ram spermatozoa
title_sort Selenium supplementation prevents DNA damage in ram spermatozoa
author Moya,Carla Fredrichsen
author_facet Moya,Carla Fredrichsen
Piagentini,Marcelo
Silva,Danilo da Cunha
Fernandes,Fábio Henrique
Salvadori,Daisy Maria Fávero
Oba,Eunice
author_role author
author2 Piagentini,Marcelo
Silva,Danilo da Cunha
Fernandes,Fábio Henrique
Salvadori,Daisy Maria Fávero
Oba,Eunice
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moya,Carla Fredrichsen
Piagentini,Marcelo
Silva,Danilo da Cunha
Fernandes,Fábio Henrique
Salvadori,Daisy Maria Fávero
Oba,Eunice
dc.subject.por.fl_str_mv chemoprevention
comet assay
semen
ovine.
topic chemoprevention
comet assay
semen
ovine.
description ABSTRACT: In the present study, we aimed to evaluate the effects of different concentrations of selenium (Se) ovine nutritional supplementation on spermatozoa DNA integrity. Thirty male ovines (age: 10 months) were used. They were fed with hay and ram food in an intensive system, which was divided into stalls (5 m long and 3 m wide) with feeding troughs, and had ad libitum access to food and water. Ovines in group 1 (G1, the negative control) received mineral salt supplementation without Se; ovines in G2 received the same mineral salt mixed with 5 mg Se (as sodium selenite)/kg mineral supplement;ovines in G3 received 10 mg Se/kg mineral supplement; ovines in G4 received 15 mg Se/kg mineral supplement; and ovines in G5 received 20 mg Se/kg mineral supplement. Ovines in all groups remained untreated for 14 days, followed by a treatment period of 56 days. Semen samples were obtained by electroejaculation. The DNA damage in semen samples was evaluated using the comet assay. The experimental design was implemented using a 5 × 5 Latin Square, i.e., five treatments and five experimental periods. The mean differences were compared using Tukey’s test at a significance level of 5%. The control group (G1) showed a high percentage of DNA damage compared to the Se-treated groups (G2-G5). Therefore, Se supplementation could decrease the basal level of DNA damage in sperm cells, suggesting that Se might exert protective effects on sperm DNA.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000100701
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782021000100701
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20200102
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.51 n.1 2021
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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