Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?

Detalhes bibliográficos
Autor(a) principal: Telles,Thalita da Costa
Data de Publicação: 2018
Outros Autores: Botelho,Ana Flávia Machado, Miranda,Ana Luisa Soares de, Soto-Blanco,Benito, Serakides,Rogéria, Ocarino,Natália de Melo, Melo,Marília Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300602
Resumo: ABSTRACT: The aim of this study was to evaluate the use of mesenchymal stem cells (MSC) in the treatment of myonecrosis induced by Bothrops alternatus venom in rats. Seventy-five male adult Wistar rats were divided into three experimental groups. G1 and G2 were injected in the gastrocnemius muscle with 120μg of B. alternatus venom, while G3 received 200μL of PBS only. Three days after the venom injection, 12 rats from G1 were treated with 5.0 x 106 MSC in PBS, whereas G2 and G3 rats received PBS. Every three days, blood and muscle samples of five animals from each group were taken for serum biochemical and pathological analyses. Histological examinations showed more intense muscle lesions following MSC treatment, characterized by disorganization and loss of muscle fibers, with focal necrosis and inflammatory infiltration by mononuclear cells. In conclusion, the use of MSC for the treatment of local damage caused by inoculation of B. alternatus venom impaired muscle regeneration and interfered in the healing process.
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spelling Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?Viperidae envenomationstem cellsratsmyonecrosismuscular repairABSTRACT: The aim of this study was to evaluate the use of mesenchymal stem cells (MSC) in the treatment of myonecrosis induced by Bothrops alternatus venom in rats. Seventy-five male adult Wistar rats were divided into three experimental groups. G1 and G2 were injected in the gastrocnemius muscle with 120μg of B. alternatus venom, while G3 received 200μL of PBS only. Three days after the venom injection, 12 rats from G1 were treated with 5.0 x 106 MSC in PBS, whereas G2 and G3 rats received PBS. Every three days, blood and muscle samples of five animals from each group were taken for serum biochemical and pathological analyses. Histological examinations showed more intense muscle lesions following MSC treatment, characterized by disorganization and loss of muscle fibers, with focal necrosis and inflammatory infiltration by mononuclear cells. In conclusion, the use of MSC for the treatment of local damage caused by inoculation of B. alternatus venom impaired muscle regeneration and interfered in the healing process.Universidade Federal de Santa Maria2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300602Ciência Rural v.48 n.3 2018reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20170760info:eu-repo/semantics/openAccessTelles,Thalita da CostaBotelho,Ana Flávia MachadoMiranda,Ana Luisa Soares deSoto-Blanco,BenitoSerakides,RogériaOcarino,Natália de MeloMelo,Marília Martinseng2018-03-19T00:00:00ZRevista
dc.title.none.fl_str_mv Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?
title Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?
spellingShingle Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?
Telles,Thalita da Costa
Viperidae envenomation
stem cells
rats
myonecrosis
muscular repair
title_short Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?
title_full Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?
title_fullStr Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?
title_full_unstemmed Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?
title_sort Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?
author Telles,Thalita da Costa
author_facet Telles,Thalita da Costa
Botelho,Ana Flávia Machado
Miranda,Ana Luisa Soares de
Soto-Blanco,Benito
Serakides,Rogéria
Ocarino,Natália de Melo
Melo,Marília Martins
author_role author
author2 Botelho,Ana Flávia Machado
Miranda,Ana Luisa Soares de
Soto-Blanco,Benito
Serakides,Rogéria
Ocarino,Natália de Melo
Melo,Marília Martins
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Telles,Thalita da Costa
Botelho,Ana Flávia Machado
Miranda,Ana Luisa Soares de
Soto-Blanco,Benito
Serakides,Rogéria
Ocarino,Natália de Melo
Melo,Marília Martins
dc.subject.por.fl_str_mv Viperidae envenomation
stem cells
rats
myonecrosis
muscular repair
topic Viperidae envenomation
stem cells
rats
myonecrosis
muscular repair
description ABSTRACT: The aim of this study was to evaluate the use of mesenchymal stem cells (MSC) in the treatment of myonecrosis induced by Bothrops alternatus venom in rats. Seventy-five male adult Wistar rats were divided into three experimental groups. G1 and G2 were injected in the gastrocnemius muscle with 120μg of B. alternatus venom, while G3 received 200μL of PBS only. Three days after the venom injection, 12 rats from G1 were treated with 5.0 x 106 MSC in PBS, whereas G2 and G3 rats received PBS. Every three days, blood and muscle samples of five animals from each group were taken for serum biochemical and pathological analyses. Histological examinations showed more intense muscle lesions following MSC treatment, characterized by disorganization and loss of muscle fibers, with focal necrosis and inflammatory infiltration by mononuclear cells. In conclusion, the use of MSC for the treatment of local damage caused by inoculation of B. alternatus venom impaired muscle regeneration and interfered in the healing process.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300602
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300602
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20170760
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.48 n.3 2018
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1749140552473903104

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