Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp.
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101973 |
Resumo: | ABSTRACT: The aim of the present study was to evaluate the effect of the colchicine and oryzalin anti-mitotic substances on the induction of tetraploid plants and foliar anatomy of two diploids clones of Solanum commersonii ssp. Nodal segments of Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. were treated with colchicine (3.5, 5.0, and 6.5mM; 72h) or oryzalin (10, 30, and 50µM; 24h). After the treatment with anti-mitotic substances, nodal segments were inoculated in the MS culture medium and cultivated in vitro (60 days). After in vitro cultivation, the plants were transferred to vases with the substrate Plantmax(r) and kept in the greenhouse (45 days). Plant ploidy level was assessed by flow cytometry and leaf anatomy was assessed by anatomic cuts. An increase was observed in the polar and equatorial diameter of stomata ("gigas effect") of the Solanum commersonii subsp. commersonii (SCC) and Solanum commersonii subsp. malmeanum (SCM) clones, which was due to the use of the anti-mitotic substances. Treatments with colchicine (6.5mM; 72h) and oryzalin (50µM; 24h) caused death of the SCC plants cultured in vitro. In the others treatments of the SCC clone, the use of oryzalin and colchicine caused production of chimeric plants. The treatment of nodal segments of SCM with oryzalin (10-50µM; 24h) was effective on induction of tetraploid plants, which can be employed in genetic breeding programs in crossbreeding with cultured potato. |
id |
UFSM-2_7d5afe8de0fd4495e2bc527762467a3c |
---|---|
oai_identifier_str |
oai:scielo:S0103-84782016001101973 |
network_acronym_str |
UFSM-2 |
network_name_str |
Ciência rural (Online) |
repository_id_str |
|
spelling |
Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp.potatocrop breedingflow citometryfoliar anatomyABSTRACT: The aim of the present study was to evaluate the effect of the colchicine and oryzalin anti-mitotic substances on the induction of tetraploid plants and foliar anatomy of two diploids clones of Solanum commersonii ssp. Nodal segments of Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. were treated with colchicine (3.5, 5.0, and 6.5mM; 72h) or oryzalin (10, 30, and 50µM; 24h). After the treatment with anti-mitotic substances, nodal segments were inoculated in the MS culture medium and cultivated in vitro (60 days). After in vitro cultivation, the plants were transferred to vases with the substrate Plantmax(r) and kept in the greenhouse (45 days). Plant ploidy level was assessed by flow cytometry and leaf anatomy was assessed by anatomic cuts. An increase was observed in the polar and equatorial diameter of stomata ("gigas effect") of the Solanum commersonii subsp. commersonii (SCC) and Solanum commersonii subsp. malmeanum (SCM) clones, which was due to the use of the anti-mitotic substances. Treatments with colchicine (6.5mM; 72h) and oryzalin (50µM; 24h) caused death of the SCC plants cultured in vitro. In the others treatments of the SCC clone, the use of oryzalin and colchicine caused production of chimeric plants. The treatment of nodal segments of SCM with oryzalin (10-50µM; 24h) was effective on induction of tetraploid plants, which can be employed in genetic breeding programs in crossbreeding with cultured potato.Universidade Federal de Santa Maria2016-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101973Ciência Rural v.46 n.11 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20150477info:eu-repo/semantics/openAccessTomé,Lívia Gracielle OliveiraSilva,Adriano Bortolotti daPinto,Cesar Augusto Brasil PereiraDavide,Lisete ChammaPereira,Dalvana SousaCarvalho,Carlos Roberto deeng2016-09-28T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp. |
title |
Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp. |
spellingShingle |
Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp. Tomé,Lívia Gracielle Oliveira potato crop breeding flow citometry foliar anatomy |
title_short |
Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp. |
title_full |
Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp. |
title_fullStr |
Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp. |
title_full_unstemmed |
Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp. |
title_sort |
Colchicine and oryzalin effects on tetraploid induction and leaf anatomy of Solanum commersonii ssp. |
author |
Tomé,Lívia Gracielle Oliveira |
author_facet |
Tomé,Lívia Gracielle Oliveira Silva,Adriano Bortolotti da Pinto,Cesar Augusto Brasil Pereira Davide,Lisete Chamma Pereira,Dalvana Sousa Carvalho,Carlos Roberto de |
author_role |
author |
author2 |
Silva,Adriano Bortolotti da Pinto,Cesar Augusto Brasil Pereira Davide,Lisete Chamma Pereira,Dalvana Sousa Carvalho,Carlos Roberto de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Tomé,Lívia Gracielle Oliveira Silva,Adriano Bortolotti da Pinto,Cesar Augusto Brasil Pereira Davide,Lisete Chamma Pereira,Dalvana Sousa Carvalho,Carlos Roberto de |
dc.subject.por.fl_str_mv |
potato crop breeding flow citometry foliar anatomy |
topic |
potato crop breeding flow citometry foliar anatomy |
description |
ABSTRACT: The aim of the present study was to evaluate the effect of the colchicine and oryzalin anti-mitotic substances on the induction of tetraploid plants and foliar anatomy of two diploids clones of Solanum commersonii ssp. Nodal segments of Solanum commersonii subsp. commersonii Dun. and Solanum commersonii subsp. malmeanum Bitt. were treated with colchicine (3.5, 5.0, and 6.5mM; 72h) or oryzalin (10, 30, and 50µM; 24h). After the treatment with anti-mitotic substances, nodal segments were inoculated in the MS culture medium and cultivated in vitro (60 days). After in vitro cultivation, the plants were transferred to vases with the substrate Plantmax(r) and kept in the greenhouse (45 days). Plant ploidy level was assessed by flow cytometry and leaf anatomy was assessed by anatomic cuts. An increase was observed in the polar and equatorial diameter of stomata ("gigas effect") of the Solanum commersonii subsp. commersonii (SCC) and Solanum commersonii subsp. malmeanum (SCM) clones, which was due to the use of the anti-mitotic substances. Treatments with colchicine (6.5mM; 72h) and oryzalin (50µM; 24h) caused death of the SCC plants cultured in vitro. In the others treatments of the SCC clone, the use of oryzalin and colchicine caused production of chimeric plants. The treatment of nodal segments of SCM with oryzalin (10-50µM; 24h) was effective on induction of tetraploid plants, which can be employed in genetic breeding programs in crossbreeding with cultured potato. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101973 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001101973 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20150477 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.46 n.11 2016 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140550529843200 |