Etiologic diagnosis of bovine infectious abortion by PCR

• Autor(a) principal: Silva,Teane Milagres Augusto da
• Data de Publicação: 2009
• Outros Autores: Oliveira,Raquel Gonzaga de, Mol,Juliana Pinto da Silva, Xavier,Mariana Noyma, Paixão,Tatiane Alves da, Cortez,Adriana, Heinemann,Marcos Bryan, Richtzenhain,Leonardo José, Lage,Andrey Pereira, Santos,Renato de Lima
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Ciência Rural
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900028
• topic: cattle; abortion; Brucella abortus; Salmonella sp.; Neospora caninum; Listeria monocytogenes

Infectious abortion is a significant cause of reproductive failure and economic losses in cattle. The goal of this study was to detect nucleic acids of several infectious agents known to cause abortion including Arcanobacterium pyogenes, Bovine Herpesvirus 1, Brucella abortus, Campylobacter fetus subsp. venerealis, Chlamydophila abortus, Leptospira sp., Listeria monocytogenes, Salmonella sp., Mycoplasma bovis, Mycoplasma bovigenitalium, Neospora caninum, and Tritrichomonas foetus. Tissue homogenates from 42 fetuses and paraffin-embedded tissues from 28 fetuses and 14 placentas/endometrium were included in this study. Brucella abortus was detected in 14.2% (12/84) of the samples. Salmonella sp. DNA was amplified from 2 fetuses, and there was one positive for Neospora caninum, and another for Listeria monocytogenes. This PCR-based approach resulted in identification of the etiology in 19% of samples, or 20% if considered fetal tissues only.