Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing

Detalhes bibliográficos
Autor(a) principal: Freitas,Loreta Brandão de
Data de Publicação: 2003
Outros Autores: Koehler-Santos,Patrícia, Salzano,Francisco Mauro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300016
Resumo: Leaves from 14 Brazilian genotypes of Triticum aestivum L. were treated with salicylic acid to induce pathogenesis-related (PR) proteins. Inter and intracellular extracts were then obtained and investigated through polyacrilamide gel electrophoresis. Seven bands were observed. Material related to two of them (of 40 and 24 kDa) occurred in intracellular spaces only. DNA from these same genotypes was then amplified through PCR using primers developed from three sequences encoding PR proteins, and compared with previously described sequences. The fragments presented homologies to PR groups 1, 3 (chitinases), and 5 (thaumatin-like). The PR3-like sequence also showed a site characteristic of PRs induced by ethylene and a portion without homology with previous sequences. No variation among genotypes were observed, either for protein extracts or DNA sequences.
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spelling Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencingTriticum aestivum L.defense proteinsgenetic variabilityLeaves from 14 Brazilian genotypes of Triticum aestivum L. were treated with salicylic acid to induce pathogenesis-related (PR) proteins. Inter and intracellular extracts were then obtained and investigated through polyacrilamide gel electrophoresis. Seven bands were observed. Material related to two of them (of 40 and 24 kDa) occurred in intracellular spaces only. DNA from these same genotypes was then amplified through PCR using primers developed from three sequences encoding PR proteins, and compared with previously described sequences. The fragments presented homologies to PR groups 1, 3 (chitinases), and 5 (thaumatin-like). The PR3-like sequence also showed a site characteristic of PRs induced by ethylene and a portion without homology with previous sequences. No variation among genotypes were observed, either for protein extracts or DNA sequences.Universidade Federal de Santa Maria2003-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300016Ciência Rural v.33 n.3 2003reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782003000300016info:eu-repo/semantics/openAccessFreitas,Loreta Brandão deKoehler-Santos,PatríciaSalzano,Francisco Mauroeng2003-11-03T00:00:00ZRevista
dc.title.none.fl_str_mv Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
title Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
spellingShingle Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
Freitas,Loreta Brandão de
Triticum aestivum L.
defense proteins
genetic variability
title_short Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
title_full Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
title_fullStr Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
title_full_unstemmed Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
title_sort Pathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencing
author Freitas,Loreta Brandão de
author_facet Freitas,Loreta Brandão de
Koehler-Santos,Patrícia
Salzano,Francisco Mauro
author_role author
author2 Koehler-Santos,Patrícia
Salzano,Francisco Mauro
author2_role author
author
dc.contributor.author.fl_str_mv Freitas,Loreta Brandão de
Koehler-Santos,Patrícia
Salzano,Francisco Mauro
dc.subject.por.fl_str_mv Triticum aestivum L.
defense proteins
genetic variability
topic Triticum aestivum L.
defense proteins
genetic variability
description Leaves from 14 Brazilian genotypes of Triticum aestivum L. were treated with salicylic acid to induce pathogenesis-related (PR) proteins. Inter and intracellular extracts were then obtained and investigated through polyacrilamide gel electrophoresis. Seven bands were observed. Material related to two of them (of 40 and 24 kDa) occurred in intracellular spaces only. DNA from these same genotypes was then amplified through PCR using primers developed from three sequences encoding PR proteins, and compared with previously described sequences. The fragments presented homologies to PR groups 1, 3 (chitinases), and 5 (thaumatin-like). The PR3-like sequence also showed a site characteristic of PRs induced by ethylene and a portion without homology with previous sequences. No variation among genotypes were observed, either for protein extracts or DNA sequences.
publishDate 2003
dc.date.none.fl_str_mv 2003-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000300016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0103-84782003000300016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.33 n.3 2003
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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