Validation of the multiplex PCR for identification of Brucella spp.

• Autor(a) principal: Orzil,Lívia de Lima
• Data de Publicação: 2016
• Outros Autores: Preis,Ingred Sales, Almeida,Iassudara Garcia de, Souza,Patrícia Gomes de, Soares Filho,Paulo Martins, Jacinto,Fabrício Barcelos, Fonseca Júnior,Antônio Augusto
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Ciência Rural
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500847
• topic: Brucellosis; molecular identification; zoonosis; Brucella; multiplex PCR

ABSTRACT: A multiplex PCR technique for detection of Brucella spp. in samples of bacterial suspension was validated as a complementary tool in the diagnosis of the disease. This technique allows the characterization of the agent without performing biochemical tests, which greatly reduces the time for a final diagnosis, and provides more security for the analyst by reducing the time of exposure to microorganisms. The validation was performed in accordance with the Manual of Diagnostic Tests from OIE (2008) and following the requirements present in the ABNT NBR ISO/IEC 17025:2005. The mPCR validated in this study identified the different species of Brucella ( Brucella abortus , B. suis , B. ovis e B. melitensis ) of bacterial suspension obtained from the slaughterhouse samples, as well as distinguished the biovars (1, 2 e 4; 3b, 5, 6 e 9) of B. abortus in grouped form and differentiated the field strains from vaccine strains, as a quick, useful and less expensive technique in diagnosis of brucellosis in Brazil.