Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain

Detalhes bibliográficos
Autor(a) principal: Oliveira Júnior,Carlos Augusto de
Data de Publicação: 2016
Outros Autores: Silva,Rodrigo Otávio Silveira, Diniz,Amanda Nádia, Pires,Prhiscylla Sadanã, Lobato,Francisco Carlos Faria, Assis,Ronie Antunes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500853
Resumo: ABSTRACT: The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain of C. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain of C. difficile (Z31) was able to prevent CDI in all animals that received it.
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spelling Prevention of Clostridium difficile infection in hamsters using a non-toxigenic straindiarrhea Clostridium difficile- associated diseasescolitisABSTRACT: The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain of C. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain of C. difficile (Z31) was able to prevent CDI in all animals that received it.Universidade Federal de Santa Maria2016-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500853Ciência Rural v.46 n.5 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20150454info:eu-repo/semantics/openAccessOliveira Júnior,Carlos Augusto deSilva,Rodrigo Otávio SilveiraDiniz,Amanda NádiaPires,Prhiscylla SadanãLobato,Francisco Carlos FariaAssis,Ronie Antunes deeng2016-10-20T00:00:00ZRevista
dc.title.none.fl_str_mv Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
spellingShingle Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
Oliveira Júnior,Carlos Augusto de
diarrhea
Clostridium difficile- associated diseases
colitis
title_short Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title_full Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title_fullStr Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title_full_unstemmed Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
title_sort Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain
author Oliveira Júnior,Carlos Augusto de
author_facet Oliveira Júnior,Carlos Augusto de
Silva,Rodrigo Otávio Silveira
Diniz,Amanda Nádia
Pires,Prhiscylla Sadanã
Lobato,Francisco Carlos Faria
Assis,Ronie Antunes de
author_role author
author2 Silva,Rodrigo Otávio Silveira
Diniz,Amanda Nádia
Pires,Prhiscylla Sadanã
Lobato,Francisco Carlos Faria
Assis,Ronie Antunes de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira Júnior,Carlos Augusto de
Silva,Rodrigo Otávio Silveira
Diniz,Amanda Nádia
Pires,Prhiscylla Sadanã
Lobato,Francisco Carlos Faria
Assis,Ronie Antunes de
dc.subject.por.fl_str_mv diarrhea
Clostridium difficile- associated diseases
colitis
topic diarrhea
Clostridium difficile- associated diseases
colitis
description ABSTRACT: The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain of C. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain of C. difficile (Z31) was able to prevent CDI in all animals that received it.
publishDate 2016
dc.date.none.fl_str_mv 2016-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500853
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000500853
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20150454
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.46 n.5 2016
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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