Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000600021 |
Resumo: | Disorders involving the nervous system have a great importance in veterinary medicine because they have a high incidence and few auxiliary tools for their diagnostic, prognostic and evaluation of the employed therapy. Today, the diagnostic is based, mainly, on the patient history and neurologic examination. Hence, evaluation of the cerebrospinal fluid elements is one of the few alternatives to clinically access the central nervous system (CNS). Even with the great usefullness of the physical-chemical and cytoscopy exams of the CSF in veterinary neurology, there are few studies concerning the stability of its elements under freezing storage. The present study was aimed at verifing the influence of temperature and period of conservation on physical-chemical characteristics of the CSF of healthy dogs. For that purpose, CSF samples were collected by puncture of the cisterna magna of dogs clinically healthy, and then were analysed for density, pH, glucorrhachia, total proteins and activity of the enzymes creatine kinase (CK) and aspartate aminotransferase (AST), after storage in different temperatures (25°C, 4°C and -4°C) and periods (immediately after collection and after 24 hours, 48 hours, a week and a month). Regarding the obtained results it was possible to verify that the parameters studied were stable up to a month of storage, under freezing at -4°C. |
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Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperaturescerebrospinal fluiddogsstoragetemperatureDisorders involving the nervous system have a great importance in veterinary medicine because they have a high incidence and few auxiliary tools for their diagnostic, prognostic and evaluation of the employed therapy. Today, the diagnostic is based, mainly, on the patient history and neurologic examination. Hence, evaluation of the cerebrospinal fluid elements is one of the few alternatives to clinically access the central nervous system (CNS). Even with the great usefullness of the physical-chemical and cytoscopy exams of the CSF in veterinary neurology, there are few studies concerning the stability of its elements under freezing storage. The present study was aimed at verifing the influence of temperature and period of conservation on physical-chemical characteristics of the CSF of healthy dogs. For that purpose, CSF samples were collected by puncture of the cisterna magna of dogs clinically healthy, and then were analysed for density, pH, glucorrhachia, total proteins and activity of the enzymes creatine kinase (CK) and aspartate aminotransferase (AST), after storage in different temperatures (25°C, 4°C and -4°C) and periods (immediately after collection and after 24 hours, 48 hours, a week and a month). Regarding the obtained results it was possible to verify that the parameters studied were stable up to a month of storage, under freezing at -4°C.Universidade Federal de Santa Maria2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000600021Ciência Rural v.36 n.6 2006reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/S0103-84782006000600021info:eu-repo/semantics/openAccessRosato,Paula NunesGama,Fernanda Gomes VelasqueSantana,Aureo Evangelistaeng2006-11-06T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures |
title |
Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures |
spellingShingle |
Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures Rosato,Paula Nunes cerebrospinal fluid dogs storage temperature |
title_short |
Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures |
title_full |
Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures |
title_fullStr |
Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures |
title_full_unstemmed |
Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures |
title_sort |
Physical-chemical analysis of the cerebrospinal fluid of healthy dogs submitted to different storage periods and temperatures |
author |
Rosato,Paula Nunes |
author_facet |
Rosato,Paula Nunes Gama,Fernanda Gomes Velasque Santana,Aureo Evangelista |
author_role |
author |
author2 |
Gama,Fernanda Gomes Velasque Santana,Aureo Evangelista |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Rosato,Paula Nunes Gama,Fernanda Gomes Velasque Santana,Aureo Evangelista |
dc.subject.por.fl_str_mv |
cerebrospinal fluid dogs storage temperature |
topic |
cerebrospinal fluid dogs storage temperature |
description |
Disorders involving the nervous system have a great importance in veterinary medicine because they have a high incidence and few auxiliary tools for their diagnostic, prognostic and evaluation of the employed therapy. Today, the diagnostic is based, mainly, on the patient history and neurologic examination. Hence, evaluation of the cerebrospinal fluid elements is one of the few alternatives to clinically access the central nervous system (CNS). Even with the great usefullness of the physical-chemical and cytoscopy exams of the CSF in veterinary neurology, there are few studies concerning the stability of its elements under freezing storage. The present study was aimed at verifing the influence of temperature and period of conservation on physical-chemical characteristics of the CSF of healthy dogs. For that purpose, CSF samples were collected by puncture of the cisterna magna of dogs clinically healthy, and then were analysed for density, pH, glucorrhachia, total proteins and activity of the enzymes creatine kinase (CK) and aspartate aminotransferase (AST), after storage in different temperatures (25°C, 4°C and -4°C) and periods (immediately after collection and after 24 hours, 48 hours, a week and a month). Regarding the obtained results it was possible to verify that the parameters studied were stable up to a month of storage, under freezing at -4°C. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000600021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782006000600021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0103-84782006000600021 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.36 n.6 2006 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140528344072192 |