Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach

Detalhes bibliográficos
Autor(a) principal: Silva,Janaina Lara da
Data de Publicação: 2015
Outros Autores: Aparecido,Christiane Ceriani, Hansen,Daiane, Pereira,Tacila Alves Muniz, Felicio,Joana D'arc, Gonçalez,Edlayne
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466
Resumo: Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the Aspergillus flavusand A. parasiticusstrains based on a morphological and molecular characterization and mycotoxigenic ability. The goat dairy diets were collected monthly from 11 goat milk farms, totaling 129 and 106 samples of concentrate and forage, respectively. For the isolation of the mycobiota the surface plating method was used. Aspergillus, Penicillium,and Fusariumwere the main fungi producing mycotoxins isolated. The morphological and molecular characterization and mycotoxigenic ability were used for A. flavusand A. parasiticusidentification. The Aspergillusspp. from feed 39% produced aflatoxins B1 and B2, 17% produced cyclopiazonic acid (CPA), 18% produced both toxins, and 42% had no toxigenic ability. Only 2.0% of the strains produced aflatoxins B1, B2, G1, and G2, but no CPA. The strains from forage were producers of aflatoxins B1 and B2 (37%), CPA (14%), 14% of both mycotoxins, whereas 49% have shown no toxigenic ability. The aflD and aflR genes were used by PCR and PCR-RFLP, respectively. The presence of toxigenic species in samples of feed for lactating goats indicates a potential risk of contamination of dairy products, if they are exposed to environmental conditions favorable to fungal growth and mycotoxin production.
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spelling Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approachtoxigenic fungiaflatoxincyclopiazonic acidfeedmolecular characterization.Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the Aspergillus flavusand A. parasiticusstrains based on a morphological and molecular characterization and mycotoxigenic ability. The goat dairy diets were collected monthly from 11 goat milk farms, totaling 129 and 106 samples of concentrate and forage, respectively. For the isolation of the mycobiota the surface plating method was used. Aspergillus, Penicillium,and Fusariumwere the main fungi producing mycotoxins isolated. The morphological and molecular characterization and mycotoxigenic ability were used for A. flavusand A. parasiticusidentification. The Aspergillusspp. from feed 39% produced aflatoxins B1 and B2, 17% produced cyclopiazonic acid (CPA), 18% produced both toxins, and 42% had no toxigenic ability. Only 2.0% of the strains produced aflatoxins B1, B2, G1, and G2, but no CPA. The strains from forage were producers of aflatoxins B1 and B2 (37%), CPA (14%), 14% of both mycotoxins, whereas 49% have shown no toxigenic ability. The aflD and aflR genes were used by PCR and PCR-RFLP, respectively. The presence of toxigenic species in samples of feed for lactating goats indicates a potential risk of contamination of dairy products, if they are exposed to environmental conditions favorable to fungal growth and mycotoxin production.Universidade Federal de Santa Maria2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466Ciência Rural v.45 n.8 2015reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20140581info:eu-repo/semantics/openAccessSilva,Janaina Lara daAparecido,Christiane CerianiHansen,DaianePereira,Tacila Alves MunizFelicio,Joana D'arcGonçalez,Edlayneeng2015-08-31T00:00:00ZRevista
dc.title.none.fl_str_mv Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach
title Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach
spellingShingle Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach
Silva,Janaina Lara da
toxigenic fungi
aflatoxin
cyclopiazonic acid
feed
molecular characterization.
title_short Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach
title_full Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach
title_fullStr Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach
title_full_unstemmed Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach
title_sort Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach
author Silva,Janaina Lara da
author_facet Silva,Janaina Lara da
Aparecido,Christiane Ceriani
Hansen,Daiane
Pereira,Tacila Alves Muniz
Felicio,Joana D'arc
Gonçalez,Edlayne
author_role author
author2 Aparecido,Christiane Ceriani
Hansen,Daiane
Pereira,Tacila Alves Muniz
Felicio,Joana D'arc
Gonçalez,Edlayne
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silva,Janaina Lara da
Aparecido,Christiane Ceriani
Hansen,Daiane
Pereira,Tacila Alves Muniz
Felicio,Joana D'arc
Gonçalez,Edlayne
dc.subject.por.fl_str_mv toxigenic fungi
aflatoxin
cyclopiazonic acid
feed
molecular characterization.
topic toxigenic fungi
aflatoxin
cyclopiazonic acid
feed
molecular characterization.
description Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the Aspergillus flavusand A. parasiticusstrains based on a morphological and molecular characterization and mycotoxigenic ability. The goat dairy diets were collected monthly from 11 goat milk farms, totaling 129 and 106 samples of concentrate and forage, respectively. For the isolation of the mycobiota the surface plating method was used. Aspergillus, Penicillium,and Fusariumwere the main fungi producing mycotoxins isolated. The morphological and molecular characterization and mycotoxigenic ability were used for A. flavusand A. parasiticusidentification. The Aspergillusspp. from feed 39% produced aflatoxins B1 and B2, 17% produced cyclopiazonic acid (CPA), 18% produced both toxins, and 42% had no toxigenic ability. Only 2.0% of the strains produced aflatoxins B1, B2, G1, and G2, but no CPA. The strains from forage were producers of aflatoxins B1 and B2 (37%), CPA (14%), 14% of both mycotoxins, whereas 49% have shown no toxigenic ability. The aflD and aflR genes were used by PCR and PCR-RFLP, respectively. The presence of toxigenic species in samples of feed for lactating goats indicates a potential risk of contamination of dairy products, if they are exposed to environmental conditions favorable to fungal growth and mycotoxin production.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20140581
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.45 n.8 2015
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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