Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466 |
Resumo: | Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the Aspergillus flavusand A. parasiticusstrains based on a morphological and molecular characterization and mycotoxigenic ability. The goat dairy diets were collected monthly from 11 goat milk farms, totaling 129 and 106 samples of concentrate and forage, respectively. For the isolation of the mycobiota the surface plating method was used. Aspergillus, Penicillium,and Fusariumwere the main fungi producing mycotoxins isolated. The morphological and molecular characterization and mycotoxigenic ability were used for A. flavusand A. parasiticusidentification. The Aspergillusspp. from feed 39% produced aflatoxins B1 and B2, 17% produced cyclopiazonic acid (CPA), 18% produced both toxins, and 42% had no toxigenic ability. Only 2.0% of the strains produced aflatoxins B1, B2, G1, and G2, but no CPA. The strains from forage were producers of aflatoxins B1 and B2 (37%), CPA (14%), 14% of both mycotoxins, whereas 49% have shown no toxigenic ability. The aflD and aflR genes were used by PCR and PCR-RFLP, respectively. The presence of toxigenic species in samples of feed for lactating goats indicates a potential risk of contamination of dairy products, if they are exposed to environmental conditions favorable to fungal growth and mycotoxin production. |
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Ciência rural (Online) |
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Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approachtoxigenic fungiaflatoxincyclopiazonic acidfeedmolecular characterization.Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the Aspergillus flavusand A. parasiticusstrains based on a morphological and molecular characterization and mycotoxigenic ability. The goat dairy diets were collected monthly from 11 goat milk farms, totaling 129 and 106 samples of concentrate and forage, respectively. For the isolation of the mycobiota the surface plating method was used. Aspergillus, Penicillium,and Fusariumwere the main fungi producing mycotoxins isolated. The morphological and molecular characterization and mycotoxigenic ability were used for A. flavusand A. parasiticusidentification. The Aspergillusspp. from feed 39% produced aflatoxins B1 and B2, 17% produced cyclopiazonic acid (CPA), 18% produced both toxins, and 42% had no toxigenic ability. Only 2.0% of the strains produced aflatoxins B1, B2, G1, and G2, but no CPA. The strains from forage were producers of aflatoxins B1 and B2 (37%), CPA (14%), 14% of both mycotoxins, whereas 49% have shown no toxigenic ability. The aflD and aflR genes were used by PCR and PCR-RFLP, respectively. The presence of toxigenic species in samples of feed for lactating goats indicates a potential risk of contamination of dairy products, if they are exposed to environmental conditions favorable to fungal growth and mycotoxin production.Universidade Federal de Santa Maria2015-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466Ciência Rural v.45 n.8 2015reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20140581info:eu-repo/semantics/openAccessSilva,Janaina Lara daAparecido,Christiane CerianiHansen,DaianePereira,Tacila Alves MunizFelicio,Joana D'arcGonçalez,Edlayneeng2015-08-31T00:00:00ZRevista |
dc.title.none.fl_str_mv |
Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach |
title |
Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach |
spellingShingle |
Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach Silva,Janaina Lara da toxigenic fungi aflatoxin cyclopiazonic acid feed molecular characterization. |
title_short |
Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach |
title_full |
Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach |
title_fullStr |
Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach |
title_full_unstemmed |
Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach |
title_sort |
Identification of toxigenic Aspergillus species from diet dairy goat using a polyphasic approach |
author |
Silva,Janaina Lara da |
author_facet |
Silva,Janaina Lara da Aparecido,Christiane Ceriani Hansen,Daiane Pereira,Tacila Alves Muniz Felicio,Joana D'arc Gonçalez,Edlayne |
author_role |
author |
author2 |
Aparecido,Christiane Ceriani Hansen,Daiane Pereira,Tacila Alves Muniz Felicio,Joana D'arc Gonçalez,Edlayne |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva,Janaina Lara da Aparecido,Christiane Ceriani Hansen,Daiane Pereira,Tacila Alves Muniz Felicio,Joana D'arc Gonçalez,Edlayne |
dc.subject.por.fl_str_mv |
toxigenic fungi aflatoxin cyclopiazonic acid feed molecular characterization. |
topic |
toxigenic fungi aflatoxin cyclopiazonic acid feed molecular characterization. |
description |
Some species of filamentous fungi that infest agricultural commodities are able to produce mycotoxins, contaminating feed and animal products. The aim of this research was to identify the mycoflora present in the feed and forage for dairy goat and to isolate and characterize the Aspergillus flavusand A. parasiticusstrains based on a morphological and molecular characterization and mycotoxigenic ability. The goat dairy diets were collected monthly from 11 goat milk farms, totaling 129 and 106 samples of concentrate and forage, respectively. For the isolation of the mycobiota the surface plating method was used. Aspergillus, Penicillium,and Fusariumwere the main fungi producing mycotoxins isolated. The morphological and molecular characterization and mycotoxigenic ability were used for A. flavusand A. parasiticusidentification. The Aspergillusspp. from feed 39% produced aflatoxins B1 and B2, 17% produced cyclopiazonic acid (CPA), 18% produced both toxins, and 42% had no toxigenic ability. Only 2.0% of the strains produced aflatoxins B1, B2, G1, and G2, but no CPA. The strains from forage were producers of aflatoxins B1 and B2 (37%), CPA (14%), 14% of both mycotoxins, whereas 49% have shown no toxigenic ability. The aflD and aflR genes were used by PCR and PCR-RFLP, respectively. The presence of toxigenic species in samples of feed for lactating goats indicates a potential risk of contamination of dairy products, if they are exposed to environmental conditions favorable to fungal growth and mycotoxin production. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000801466 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20140581 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.45 n.8 2015 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140548145381376 |