ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Ciência Rural |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150 |
Resumo: | This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats. |
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ω-conotoxin MVIIA intralesional injection in spinal cord injury in ratsMVIIAcone snailcell viabilityglutamatereactive oxygen specieslipid peroxidationThis study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats.Universidade Federal de Santa Maria2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150Ciência Rural v.46 n.1 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20141203info:eu-repo/semantics/openAccessOliveira,Karen Maciel deBinda,Nancy ScarduaLavor,Mário Sérgio LimaSilva,Carla Maria OsórioRosado,Isabel RodriguesTaguchi,Tatiana MalagoliAlves,Endrigo Gabellini LeonelMelo,Marília MartinsGomez,Marcus ViníciusMelo,Eliane Gonçalves deeng2016-01-20T00:00:00ZRevista |
dc.title.none.fl_str_mv |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
title |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
spellingShingle |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats Oliveira,Karen Maciel de MVIIA cone snail cell viability glutamate reactive oxygen species lipid peroxidation |
title_short |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
title_full |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
title_fullStr |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
title_full_unstemmed |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
title_sort |
ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats |
author |
Oliveira,Karen Maciel de |
author_facet |
Oliveira,Karen Maciel de Binda,Nancy Scardua Lavor,Mário Sérgio Lima Silva,Carla Maria Osório Rosado,Isabel Rodrigues Taguchi,Tatiana Malagoli Alves,Endrigo Gabellini Leonel Melo,Marília Martins Gomez,Marcus Vinícius Melo,Eliane Gonçalves de |
author_role |
author |
author2 |
Binda,Nancy Scardua Lavor,Mário Sérgio Lima Silva,Carla Maria Osório Rosado,Isabel Rodrigues Taguchi,Tatiana Malagoli Alves,Endrigo Gabellini Leonel Melo,Marília Martins Gomez,Marcus Vinícius Melo,Eliane Gonçalves de |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Karen Maciel de Binda,Nancy Scardua Lavor,Mário Sérgio Lima Silva,Carla Maria Osório Rosado,Isabel Rodrigues Taguchi,Tatiana Malagoli Alves,Endrigo Gabellini Leonel Melo,Marília Martins Gomez,Marcus Vinícius Melo,Eliane Gonçalves de |
dc.subject.por.fl_str_mv |
MVIIA cone snail cell viability glutamate reactive oxygen species lipid peroxidation |
topic |
MVIIA cone snail cell viability glutamate reactive oxygen species lipid peroxidation |
description |
This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0103-8478cr20141203 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
Ciência Rural v.46 n.1 2016 reponame:Ciência Rural instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Ciência Rural |
collection |
Ciência Rural |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1749140548592074752 |