ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats

Detalhes bibliográficos
Autor(a) principal: Oliveira,Karen Maciel de
Data de Publicação: 2016
Outros Autores: Binda,Nancy Scardua, Lavor,Mário Sérgio Lima, Silva,Carla Maria Osório, Rosado,Isabel Rodrigues, Taguchi,Tatiana Malagoli, Alves,Endrigo Gabellini Leonel, Melo,Marília Martins, Gomez,Marcus Vinícius, Melo,Eliane Gonçalves de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Ciência Rural
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150
Resumo: This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats.
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spelling ω-conotoxin MVIIA intralesional injection in spinal cord injury in ratsMVIIAcone snailcell viabilityglutamatereactive oxygen specieslipid peroxidationThis study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats.Universidade Federal de Santa Maria2016-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150Ciência Rural v.46 n.1 2016reponame:Ciência Ruralinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSM10.1590/0103-8478cr20141203info:eu-repo/semantics/openAccessOliveira,Karen Maciel deBinda,Nancy ScarduaLavor,Mário Sérgio LimaSilva,Carla Maria OsórioRosado,Isabel RodriguesTaguchi,Tatiana MalagoliAlves,Endrigo Gabellini LeonelMelo,Marília MartinsGomez,Marcus ViníciusMelo,Eliane Gonçalves deeng2016-01-20T00:00:00ZRevista
dc.title.none.fl_str_mv ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
title ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
spellingShingle ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
Oliveira,Karen Maciel de
MVIIA
cone snail
cell viability
glutamate
reactive oxygen species
lipid peroxidation
title_short ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
title_full ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
title_fullStr ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
title_full_unstemmed ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
title_sort ω-conotoxin MVIIA intralesional injection in spinal cord injury in rats
author Oliveira,Karen Maciel de
author_facet Oliveira,Karen Maciel de
Binda,Nancy Scardua
Lavor,Mário Sérgio Lima
Silva,Carla Maria Osório
Rosado,Isabel Rodrigues
Taguchi,Tatiana Malagoli
Alves,Endrigo Gabellini Leonel
Melo,Marília Martins
Gomez,Marcus Vinícius
Melo,Eliane Gonçalves de
author_role author
author2 Binda,Nancy Scardua
Lavor,Mário Sérgio Lima
Silva,Carla Maria Osório
Rosado,Isabel Rodrigues
Taguchi,Tatiana Malagoli
Alves,Endrigo Gabellini Leonel
Melo,Marília Martins
Gomez,Marcus Vinícius
Melo,Eliane Gonçalves de
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Karen Maciel de
Binda,Nancy Scardua
Lavor,Mário Sérgio Lima
Silva,Carla Maria Osório
Rosado,Isabel Rodrigues
Taguchi,Tatiana Malagoli
Alves,Endrigo Gabellini Leonel
Melo,Marília Martins
Gomez,Marcus Vinícius
Melo,Eliane Gonçalves de
dc.subject.por.fl_str_mv MVIIA
cone snail
cell viability
glutamate
reactive oxygen species
lipid peroxidation
topic MVIIA
cone snail
cell viability
glutamate
reactive oxygen species
lipid peroxidation
description This study aimed to investigate the neuroprotective effect of ω-conotoxin MVIIA (MVIIA) intralesional application in rats submitted to spinal cord injury. Male Wistar rats, weighing 300g±23.4, were distributed in five groups: negative control (SHAM), placebo (PLA), 5μM MVIIA, 10μM MVIIA and 20μM MVIIA MVIIA. After laminectomy of the 12th thoracic vertebra (SHAM), the PLA, 5μM MVIIA, 10μM MVIIA and 20μM MVIIA groups were subjected to acute compressive spinal cord trauma for five minutes, and then five minutes later, the animals received specific treatment in a standard total volume of 2µL, by intralesional route, using sterile PBS as placebo. Locomotor activity was assayed using Basso Beattie Bresnahan (BBB) scale to show the patterning of SCI. With 48 hours of injury, the animals were euthanized, the liquor sample was collected in atlantooccipital space, and also the spinal segment, including the epicenter and caudal region to injury. Assays were performed for mitochondrial viability, serum glutamate, production of reactive oxygen species (ROS) and lipid peroxidation (LP) were performed. The study design was randomized and the data submitted to ANOVA and comparison of means by SNK test, and data from BBB scale were evaluated using Kruskal-Wallis test (P<0.05). There was no significant difference between groups in BBB scores. The MVIIA did not promote decrease in the levels of glutamate, ROS, LP, and did not preserve the mitochondria in the intralesional application five minutes after spinal cord injury in rats.
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100150
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0103-8478cr20141203
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Rural v.46 n.1 2016
reponame:Ciência Rural
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Rural
collection Ciência Rural
repository.name.fl_str_mv
repository.mail.fl_str_mv
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