Brazilian ground beef authentication by multiplex polymerase chain reaction

• Autor(a) principal: Oliveira,Andrey Carlos do Sacramento de
• Data de Publicação: 2018
• Outros Autores: Pedroso,Silvia Cristina da Silva, Cardilli,Diogo José, Leite,Fábio Pereira Leivas, Ferreira,Gabrielle Virgínia Lopes, Silva,Andréia Silva da, Roos,Talita Bandeira, Moraes,Carina Martins de, Sousa,Roberta Sales, Monteiro,Roseli do Socorro Dias
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Ciência Rural
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000200652
• topic: species identification; Bos taurus; Bubalusbubalis; DNA; adulteration

ABSTRACT: The aim of the present study was to assess the efficacy ofmultiplex PCR in detecting the adulterationof commercially available ground beefvia addition and/orsubstitution ofground buffalo meat. Experimentally adulterated ground beefsamples were prepared in triplicate, and dilutions of DNA from Bos taurus and Bubalusbubalis were prepared to determine the detection limit of the method. Concurrently, 91 ground meatsamples sold as “ground beef” were collected from differentstores in northern Brazil andanalyzed bymultiplex PCR. Buffalo DNA was detected in 17.5% of the collected ground meat samples.Our results showed that multiplex PCR is an efficient method for detectingthe incorporation of groundbuffalo meatatpercentages ranging from 10 to 100% and the incorporation of beef at percentages ranging from0.1 to 100% intoground meat samples.